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Arica, Chile

Huanca-Mamani W.,U. Tarapaca | Salvatierra Martinez R.,U. Tarapaca | Sepulveda-Chavera G.,U. Tarapaca
Idesia | Year: 2014

Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

The chemical quality of humus produced by the Californian red worm drawn from four different preparations compost organic waste was evaluated. We worked with four treatments: T0 (100% soil); T1 (compost tomato: capsicum) (1:1 w / w); T2 (fresh tomato, capsicum residues: soil) (1:1 w / w); T3 (compost ornamental plants Ficus: Hibiscus: Cynodon) (1:1:3 w / w) and T4 (olive compost: tomato) (1:1 w / w). Each treatment was chemically characterized to start the process and after three months. The results were statistically analyzed and the means were compared with the multiple range test of Tukey (p <0.05). The humus obtained in T4 (mixture of herbaceous and woody) presented the best chemical indicators such as reduction of 51.32% EC, 50% reduction in acidity, C / N ratio of 16, among others.

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