Lin P.-C.,National Dong Hwa University |
Lin S.-Z.,China Medical University at Taichung |
Chen Y.-L.,National Ilan University |
Chang J.-S.,China Medical University at Taichung |
And 14 more authors.
Annals of Surgical Oncology | Year: 2011
Background: Telomerase is widely expressed in most human cancers, but is almost undetectable in normal somatic cells and is therefore a potential drug target. Using the human telomerase promoter platform, the naturally occurring compound butylidenephthalide (BP) was selected for subsequent investigation of antitumor activity in vitro and in vivo. Methods: We treated human glioblastoma cells with BP and found a dose-dependent decrease in human telomerase reverse transcriptase (hTERT) mRNA expression and a concomitant increase in p16 and p21 expression. Because c-Myc and Sp1 are involved in transcriptional regulation of hTERT, the effect of BP on c-Myc and Sp1 expression was examined. Results: Using electrophoretic mobility shift assays and western blotting, we showed that BP represses hTERT transcriptional activity via downregulation of Sp1 expression. Using the telomerase repeat amplification protocol, an association between BP concentration and suppression of telomerase activity, induction of human glioblastoma senescence, and inhibition of cellular proliferation was identified. This was supported by a mouse xenograft model, in which BP repressed telomerase and inhibited tumor proliferation, resulting in tumor senescence. Overexpression of hTERT restored telomerase activity in human glioblastoma cells and overcame replicative senescence. Conclusions: These findings suggest that BP inhibits proliferation and induces senescence in human glioblastomas by downregulating hTERT expression and consequently telomerase activity. This is the first study to describe regulation of telomerase activity by BP in human glioblastomas. © Society of Surgical Oncology 2011.
Yang K.-L.,Buddhist Tzu Chi Stem Cells Center |
Yang K.-L.,Tzu Chi University |
Lee J.-T.,Tzu Chi University |
Pang C.-Y.,Buddhist Tzu Chi Medical Center |
And 7 more authors.
Cellular and Molecular Biology Letters | Year: 2012
Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke. © 2012 Versita Warsaw and Springer-Verlag Wien.
Chen S.-Y.,Buddhist Tzu Chi General Hospital |
Chen S.-Y.,Tzu Chi University |
Tsai S.-T.,Buddhist Tzu Chi General Hospital |
Tsai S.-T.,Tzu Chi University |
And 8 more authors.
Stereotactic and Functional Neurosurgery | Year: 2011
Background: The efficacy and feasibility of bilateral subthalamic deep brain stimulation (STN-DBS) for Parkinson's disease (PD) under general anesthesia (GA) has not been evaluated. Objective: We compared the outcome of patients under GA with those who were operated on under local anesthesia (LA). Material and Methods: Thirty-three patients were assigned to the GA group (desflurane) and 19 patients were assigned to the LA group. Microelectrode recording (MER) was performed in both groups. The surgical outcomes of the patients were evaluated using the Unified Parkinson's Disease Rating Scale (UPDRS) after at least 12 months after surgery. Results: Postoperatively, there was no significant difference on the UPDRS scores in either groups. A significant deterioration in cognitive function in the GA group was observed (p = 0.017). The recorded electrode coordinates, the average tracts for the MER, and STN depth were comparable in both groups. The overall incidence of adverse effects did not show any difference except that the in-cidence of sialorrhea and dysarthria was significantly higher in the GA group. Conclusion: Desflurane GA was shown to be a good alternative anesthetic method for PD patients undergoing DBS. Although the motor outcomes were comparable, a significant cognitive decline may be seen in the GA group with a higher occurrence of stimulation side effects. © 2011 S. Karger AG, Basel.
Chang Y.-H.,Buddhist Tzu Chi General Hospital |
Chang Y.-H.,Tzu Chi University |
Yang S.-H.,Buddhist Tzu Chi General Hospital |
Wang T.-F.,Buddhist Tzu Chi General Hospital |
And 5 more authors.
Pediatrics and Neonatology | Year: 2011
Background: Cord blood banking has become more popular in recent years. Checking cord blood complete blood count (CBC) and white blood cell (WBC) differential counts (DCs) is essential before cryopreserving the cord blood units. Therefore, establishing the normal reference values of cord blood CBC and WBC DC is important in clinical practice and research. Objectives: To obtain a large-scale population-based normal CBC and WBC DC reference values of healthy neonates' cord blood from a public cord blood bank and to investigate the influence of the gender and delivery route. Methods: From September 2001 to November 2006, the cord blood of healthy Taiwanese neonates with gestational age 36 weeks and more were collected by Tzu Chi Cord Blood Bank with written informed consents. All cord blood samples were analyzed by Sysmex XE2100 automated hematology analyzer (Sysmex Corporation, Kobe, Japan) to obtain the CBC. The WBC DC was calculated by manual method. We used Student's t test and Mann-Whitney U test for investigating the influences of gender and delivery route on the CBC and WBC DC reference values. The results were presented by mean ± standard deviation or 2.5-97.5th percentiles. Results: In the study period, totally 5602 cord blood samples were collected eligibly for analysis. The cord blood CBC and WBC DC normal reference values were calculated. The female neonates had significantly higher mean corpuscular volume, platelet count, and WBC count, but lower red blood cell (RBC) count, hemoglobin (Hb), hematocrit, and mean corpuscular Hb concentration values (p < 0.001). Newborns through vaginal delivery had significantly higher RBC count, Hb, hematocrit, platelet count, and WBC count (p < 0.001). The percentages of some different types WBC were significantly influenced by gender and delivery routes. Male babies had higher lymphocyte, monocyte, eosinophil, basophil, and nucleated RBC ratios than the female neonates. Newborns through cesarean section had significantly lower neutrophil, monocyte, and nucleated RBC ratios, but higher lymphocyte and eosinophil ratios, than newborns through vaginal delivery. Conclusion: We successfully obtained the normal CBC and WBC DC reference values of the cord blood in Taiwan. Gender and delivery routes were important confounding factors that influenced the cord blood CBC and WBC DC values. Copyright © 2011, Taiwan Pediatric Association.
Lin H.-Z.,Buddhist Tzu Chi General Hospital |
Pang C.-Y.,Buddhist Tzu Chi General Hospital |
Pang C.-Y.,Tzu Chi University |
Chen S.-P.,Buddhist Tzu Chi Stem Cells Center |
And 2 more authors.
Kaohsiung Journal of Medical Sciences | Year: 2012
In this report, we describe a Taiwanese (Han Chinese) family with Leber's hereditary optic neuropathy. The family carried a mitochondrial DNA mutation (mtDNA m.14484T>C) associated with spontaneous visual improvement. A 15-year-old boy from this family was diagnosed with Leber's hereditary optic neuropathy 6 months after losing his vision. His vision recovered after 8 months of supportive treatment. His mother, older brother, and two sisters also had the same mutation and had previously experienced vision loss. In this family, there was no male predominance. © 2012, Elsevier Taiwan LLC. All rights reserved.
Wu Y.-F.,Tzu Chi General Hospital |
Chiu Y.-W.,Buddhist Tzu Chi Stem Cells Center |
Wang T.-F.,Tzu Chi General Hospital |
Wang T.-F.,Buddhist Tzu Chi Stem Cells Center |
Wang T.-F.,Tzu Chi University
Journal of Internal Medicine of Taiwan | Year: 2012
Allogeneic hematopoietic stem cell transplantation is now an important treatment for numerous hematologic diseases. Donors must be able to provide informed consent without coercion or pressure. Donors and graft products should be evaluated for the potential to transmit infections and other diseases that jeopardized the donor's and recipient's health during stem cell collection. Understanding of the potential physical and psychological complications of donation and the factors that may increase these risks is important. About the stem cell from bone marrow harvest, general anaesthesia is necessary, and the bone marrow will be aspirated from posterior crest area. The common side effect includes back pain and fatigue. If using the peripheral stem cell collection, 5-days G-CSF injection is necessary. The commone side effect includes bone pain, fatigue and headache. Donation of hematopoietic stem cells, either bone marrow or peripheral blood collection, is a well-established and safe procedure. Serious adverse events are uncommon for healthy donors. Nevertheless, all donors must be carefully evaluated and fully informed before donation.
Chen S.-H.,Tzu Chi General Hospital |
Chen S.-H.,Tzu Chi University |
Chen S.-H.,Buddhist Tzu Chi Stem Cells Center |
Yang S.-H.,Tzu Chi General Hospital |
And 14 more authors.
International Journal of Hematology | Year: 2011
Granulocyte colony-stimulating factor (G-CSF) is now widely used for stem cell mobilization. We evaluated the role of post-G-CSF white blood cell (WBC) counts and donor factors in predicting adverse events and yields associated with mobilization. WBC counts were determined at baseline, after the third and the fifth dose of G-CSF in 476 healthy donors. Donors with WBC ≥ 50 × 103/μL post the third dose of G-CSF experienced more fatigue, myalgia/arthralgia, and chills, but final post-G-CSF CD34+ cell counts were similar. Although the final CD34+ cell count was higher in donors with WBC ≥ 50 × 103/μL post the fifth G-CSF, the incidence of side effects was similar. Females more frequently experienced headache, nausea/anorexia, vomiting, fever, and lower final CD34+ cell count than did males. Donors with body mass index (BMI) ≥ 25 showed higher incidences of sweat and insomnia as well as higher final CD34+ cell counts. Donor receiving G-CSF ≥ 10 μg/kg tended to experience bone pain, headache and chills more frequently. Multivariate analysis indicated that female gender is an independent factor predictive of the occurrence of most side effects, except for ECOG > 1 and chills. Higher BMI was also an independent predictor for fatigue, myalgia/arthralgia, and sweat. Higher G-CSF dose was associated with bone pain, while the WBC count post the third G-CSF was associated with fatigue only. In addition, one donor in the study period did not complete the mobilization due to suspected anaphylactoid reaction. Observation for 1 h after the first injection of G-CSF is required to prevent complications from unpredictable side effects. © 2011 The Japanese Society of Hematology.
PubMed | Buddhist Tzu Chi Stem Cells Center
Type: Journal Article | Journal: Cellular & molecular biology letters | Year: 2012
Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and -III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.
PubMed | Buddhist Tzu Chi Stem Cells Center
Type: Journal Article | Journal: International journal of immunogenetics | Year: 2010
HLA-B*5214, a novel rare allele of HLA-B*52 variant, was found in a Taiwanese volunteer bone marrow donor by sequence-based typing method. The sequence of B*5214 is identical to that of B*520101 in exon 2 but differs from B*520101 in exon 3 at nucleotide positions 419 A-->T and 435 A-->G. Alteration of these two nucleotides resulted an amino acid substitution at amino acid residue 116 Y-->F ( TAC-->TTC) and a silent exchange at residue 121 K-->K (AAA-->AAG).