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Dassoulas K.,Tzaneion Hospital | Gazouli M.,National and Kapodistrian University of Athens | Theodoropoulos G.,National and Kapodistrian University of Athens | Christoni Z.,National and Kapodistrian University of Athens | And 6 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2010

Purpose and methods: Vascular endothelial growth factor (VEGF) overexpression has been associated with advanced stage and poor survival in several cancers. Additionally, endoglin was proposed as a marker of neovascularization in solid malignancies. The aim of this study was to evaluate the association between the VEGF and endoglin expression in colorectal carcinoma patients, as well as to correlate the VEGF and endoglin expression with standard parameters, to deWne their potential prognostic role. VEGF and endoglin expression were evaluated in 99 unrelated patients with colorectal cancer using immunohistochemistry. Results: Vascular endothelial growth factor and endoglobin expression were positively interrelated. No signiWcant correlation of VEGF and endoglin expression with clinicopathological parameters was observed in our cases. The Kaplan-Meier survival curves have demonstrated a clear association of cancer-speciWc overall survival with high VEGF, as well as high endoglin expression. Conclusion: Our results support that VEGF and endoglin act as two valuable indicators of prognosis.


Dikeakos P.,Tzaneion Hospital | Theodoropoulos G.,Hippocratio General Hospital | Rizos S.,Tzaneion Hospital | Tzanakis N.,Asclepieion Voulas | And 2 more authors.
Molecular Biology Reports | Year: 2014

MicroRNAs (miRNAs) play an important role in regulating gene expression at the post-transcriptional level and are involved in numerous physiological processes. Accumulating evidence suggests that single-nucleotide polymorphisms (SNPs) in human miRNA genes may affect miRNA biogenesis pathway and influence the susceptibility to several diseases such as cancer. The aim of the study was to investigated whether three common miRNA polymorphisms [miR-146a C>G (rs2910164), miR-149 T>C (rs2292832), and miR-196a2 T>C (rs11614913)] are associated with the susceptibility and prognosis of gastric cancer (GC) in the Greek population. The three mRNA SNPs were identified in a case-control study (163 patients; 480 controls) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. We found that the risk for GC was significantly higher for the carriers of miR-149 rs2292832CC (p = 0.009) and miR-196a2 rs11614913CC (p < 0.0001) genotypes, as well as for the carriers of the rs2910164/rs2292832/rs11614913 CCC and GTC haplotype (p < 0.0001 and p = 0.03, respectively). The rs2910164/rs2292832/rs11614913 CTT and CCT haplotypes seems to have a protective role against GC (p = 0.002 and p = 0.001, respectively). Our data demonstrate that specific miRNA SNPs are associated with GC susceptibility in the Greek population. © Springer Science+Business Media 2013.


Liamarkopoulos E.,Tzaneion Hospital | Gazouli M.,National and Kapodistrian University of Athens | Aravantinos G.,Agioi Anargyroi Oncology Hospital | Tzanakis N.,National and Kapodistrian University of Athens | And 3 more authors.
Gastric Cancer | Year: 2011

Background Caspase-8 (CASP8) and caspase-9 (CASP9) play crucial roles in regulating apoptosis, and their functional polymorphisms may alter cancer risk. Our aim was to investigate the association of CASP8 and CASP9 gene polymorphisms with gastric cancer (GC) susceptibility. Methods We undertook a case-control study of 88 GC cases and 480 controls to investigate the association between CASP8 -652 6N ins/del and CASP9 -1263 A>G polymorphisms and GC susceptibility by a polymerase chain reaction (PCR)-restriction fragment length polymorphism method. Results CASP8 -652 6N ins/del polymorphism and CASP9 -1263 GG genotype were observed to be significantly associated with a reduced risk of GC. No significant association was observed between CASP8 -652 6N ins/del and CASP9 -1263 A>G polymorphisms and tumor characteristics. However, both CASP8 del/del and CASP9 -1263 GG genotypes were associated with increased overall survival in GC patients. Conclusions The CASP8 -652 6N ins/del and the CASP9 -1263 A>G polymorphisms were observed to play a protective role in GC predisposition. © The International Gastric Cancer Association and The Japanese Gastric Cancer Association 2011.


Liarmakopoulos E.,Tzaneion Hospital | Theodoropoulos G.,National and Kapodistrian University of Athens | Vaiopoulou A.,National and Kapodistrian University of Athens | Rizos S.,Tzaneion Hospital | And 4 more authors.
Molecular Medicine Reports | Year: 2013

Stromal-cell derived factor-1 (SDF-1), a CXC chemokine, is important for growth, angiogenesis and metastasis of tumor cells. The SDF1-3'A polymorphism has been investigated in various types of cancer; however, no information is currently available on its role in gastric cancer. Survivin is a member of the inhibitor of apoptosis family of proteins and has a genetic polymorphism (-31G/C) located in the CDE/CHR repressor element of its promoter. In this study, 88 gastric cancer patients and 480 normal healthy control subjects were investigated for the genotype and allelic SDF1-3'A and survivin-31G/C frequencies using polymerase chain reaction-restriction fragment length polymorphism. The SDF1-3'A genotype frequencies for GG, GA and AA were 44.32, 48.86 and 6.92% in patients and 42.71, 47.71 and 9.58% in healthy subjects, respectively. GA+AA genotype frequency and A allele distribution were not identified as significantly different between gastric cancer cases and controls. The survivin frequencies for GG, GC and CC were 20.45, 50 and 29.54% in patients and 33.96, 45 and 21.04% in healthy subjects, respectively. The C carriers (GC+CC genotype) and the C allele were over-represented among the gastric cancer cases (P=0.013 and P=0.0083, respectively). Overall, no statistically significant association was identified for SDF-1 and survivin gene examined alleles and genotypes and any parameter investigated, (e.g., stage, differentiation status and survival). The survivin promoter-31G/C polymorphism may confer an increased susceptibility to gastric cancer, while the SDF1-3'A polymorphism may not be a candidate genetic variant to select individuals at higher risk of developing gastric cancer.


Dikaiakos P.,Tzaneion Hospital | Gazouli M.,National and Kapodistrian University of Athens | Rizos S.,Tzaneion Hospital | Zografos G.,National and Kapodistrian University of Athens | Theodoropoulos G.E.,National and Kapodistrian University of Athens
Cancer biomarkers : section A of Disease markers | Year: 2015

BACKGROUND: Aberrant expression and structural alteration of miRNAs are considered to participate in cancer development. It has been suggested that common single-nucleotide polymorphisms (SNPs) in miRNAs are associated with susceptibility to several human diseases including colorectal cancer (CRC).METHODS: A case-control study at 157 CRC patients and 299 healthy controls of Greek origin was undertaken in order to investigate the association between the genotype and allelic frequencies of three common SNPs (rs2910164, rs11614913 and rs3746444) in pre-miRNAs, miR-146a, miR-196a2 and miR-499.RESULTS: The risk for CRC was significantly higher at the carriers of miR-146a rs2910164 CC genotype and C allele (p=0.02 and p< 0.001, respectively). None of the other performed analysis showed any statistically significant results.CONCLUSIONS: Our findings suggest that the rs2910164 polymorphism in pre-miRNA, miR-146a may be associated with the risk of CRC.


Stamatiou K.N.,Tzaneion Hospital
Urology Journal | Year: 2011

Purpose: To discuss the issue of screening for prostate cancer in elderly individuals. The impact of life expectancy on the choice of treatment in both patients and health care providers has been investigated as well. Materials and Methods: We identified studies published from 1990 onwards by searching the MEDLINE database of the National Library of Medicine. Initial search terms were "localized prostate cancer" and "early stage prostate cancer" combined with "elderly patients, life expectancy, palliative, curative, quality of life, watchful waiting, radical prostatectomy, brachytherapy, and external beam radiotherapy". Results: Despite the decrease in prostate carcinoma-specific mortality, the use of prostate-specific antigen (PSA) has been shown to increase the prostate cancer detection rate with a shift to detection at earlier and less invasive pathological stages, overriding concerns about over-diagnosis and overtreating. However, PSA screening is mainly offered to younger individuals, and older patients are more likely to have progressive disease and high-risk prostate cancer at diagnosis. Given that PSA screening diagnoses mainly curable, early prostate cancer, screening decision could be offered to otherwise healthy elderly patients who are likely to benefit from aggressive treatment. Conclusion:Prostate-specific antigen screening is not officially recommended and most scientific associations promote shared decision making. While PSA screening decision is currently based on physician's judgment, it is clear that a strict age cut-off of 75 years reduces over-screening, but also prohibits screening in healthy older men with a long life expectancy.


PubMed | Tzaneion Hospital
Type: Journal Article | Journal: Molecular biology reports | Year: 2014

MicroRNAs (miRNAs) play an important role in regulating gene expression at the post-transcriptional level and are involved in numerous physiological processes. Accumulating evidence suggests that single-nucleotide polymorphisms (SNPs) in human miRNA genes may affect miRNA biogenesis pathway and influence the susceptibility to several diseases such as cancer. The aim of the study was to investigated whether three common miRNA polymorphisms [miR-146a C>G (rs2910164), miR-149 T>C (rs2292832), and miR-196a2 T>C (rs11614913)] are associated with the susceptibility and prognosis of gastric cancer (GC) in the Greek population. The three mRNA SNPs were identified in a case-control study (163 patients; 480 controls) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. We found that the risk for GC was significantly higher for the carriers of miR-149 rs2292832CC (p=0.009) and miR-196a2 rs11614913CC (p<0.0001) genotypes, as well as for the carriers of the rs2910164/rs2292832/rs11614913 CCC and GTC haplotype (p<0.0001 and p=0.03, respectively). The rs2910164/rs2292832/rs11614913 CTT and CCT haplotypes seems to have a protective role against GC (p=0.002 and p=0.001, respectively). Our data demonstrate that specific miRNA SNPs are associated with GC susceptibility in the Greek population.


PubMed | Tzaneion Hospital
Type: Journal Article | Journal: Molecular medicine reports | Year: 2013

Stromal-cell derived factor-1(SDF-1), a CXC chemokine, is important for growth, angiogenesis and metastasis of tumor cells. The SDF1-3A polymorphism has been investigated in various types of cancer; however, no information is currently available on its role in gastric cancer. Survivin is a member of the inhibitor of apoptosis family of proteins and has a genetic polymorphism (-31G/C) located in the CDE/CHR repressor element of its promoter. In this study, 88gastric cancer patients and 480normal healthy control subjects were investigated for the genotype and allelic SDF1-3A and survivin -31G/C frequencies using polymerase chain reactionrestriction fragment length polymorphism. The SDF1-3A genotype frequencies for GG, GA and AA were 44.32, 48.86 and 6.92% in patients and 42.71, 47.71 and 9.58% in healthy subjects, respectively. GA+AA genotype frequency and Aallele distribution were not identified as significantly different between gastric cancer cases and controls. The survivin frequencies for GG, GC and CC were 20.45, 50 and 29.54% in patients and 33.96, 45 and 21.04% in healthy subjects, respectively. The Ccarriers (GC+CC genotype) and the C allele were over-represented among the gastric cancer cases (P=0.013 and P=0.0083, respectively). Overall, no statistically significant association was identified for SDF-1 and survivin gene examined alleles and genotypes and any parameter investigated, (e.g., stage, differentiation status and survival). The survivin promoter -31G/C polymorphism may confer an increased susceptibility to gastric cancer, while the SDF1-3A polymorphism may not be a candidate genetic variant to select individuals at higher risk of developing gastric cancer.


PubMed | Tzaneion Hospital
Type: Journal Article | Journal: Journal of cancer research and clinical oncology | Year: 2010

Vascular endothelial growth factor (VEGF) overexpression has been associated with advanced stage and poor survival in several cancers. Additionally, endoglin was proposed as a marker of neovascularization in solid malignancies. The aim of this study was to evaluate the association between the VEGF and endoglin expression in colorectal carcinoma patients, as well as to correlate the VEGF and endoglin expression with standard parameters, to define their potential prognostic role. VEGF and endoglin expression were evaluated in 99 unrelated patients with colorectal cancer using immunohistochemistry.Vascular endothelial growth factor and endoglobin expression were positively interrelated. No significant correlation of VEGF and endoglin expression with clinicopathological parameters was observed in our cases. The Kaplan-Meier survival curves have demonstrated a clear association of cancer-specific overall survival with high VEGF, as well as high endoglin expression.Our results support that VEGF and endoglin act as two valuable indicators of prognosis.

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