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West Lafayette, IN, United States

Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase II | Award Amount: 451.54K | Year: 2015

DESCRIPTION provided by applicant With recent technical advances multiple important signaling pathways that may be the causes of human malignancy have continuously been discovered and dissected The vast majority of these signaling pathways involve reversible protein phosphorylation and the information on the location and dynamics of phosphorylation provides important mechanisms on how the signaling networks function and interact While translational research gradually shifts from lab models to clinical samples with the ultimate goal of identifying cancer biomarkers a simple and reliable phosphorylation assay method is still missing for routine detection of phosphorylation in complex and typically heterogeneous clinical samples Through this NIH SBIR Phase II study we will further develop a novel strategy for phosphorylation assay termed pIMAGO phospho imaging which has recently been introduced and commercialized by us into highly effective products for simple and routine phosphorylation assays This novel design takes advantage of not only the quantum size properties of the soluble nanoparticles but also of the multi functionalized nature of the molecule allowing for highly selective sensitive and simple qualitative and quantitative assessment of protein phosphorylation without the use of either radioactive isotopes or expensive phosphospecific antibodies Due to its size and unique properties it also offers the capability for multiplexed detection of phosphorylation and total protein amount simultaneously We propose to optimize the technology for universal on membrane phosphoprotein detection in routine biological and biomedical research In addition we will develop a set of novel pIMAGO based strategies for multiplexed detection of phosphorylation in antibody microarray and functional reversed phase array formats The new applications will add another dimension to traditional biomedical research and development PUBLIC HEALTH RELEVANCE Protein phosphorylation relates to the onset and development of many cancer types and a highly efficient technology for phosphorylation analysis is critical for cancer research This NIH SBIR Phase II project will support an effort to develop an innovative phosphorylation analysis technology into commercial products that equip researchers with powerful tools and new directions to combat the devastating disease


Grant
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 619.95K | Year: 2013

This Small Business Innovation Research (SBIR) Phase II project addresses the unmet needs for effective analysis of protein phosphorylation, a process where a phosphate group is added to a protein to change its function. Protein phosphorylation is a crucial modification of proteins; its abnormalities have been implicated in many diseases. Therefore, assessing the phosphorylation status of individual proteins or classes of proteins, qualitatively or quantitatively, has become a routine but extremely important step in the majority of life science research labs. Existing technologies have glaring deficiencies, including low reproducibility, poor recovery, high cost, reduced selectivity and prolonged experiment time. The platform technology to be developed during this Phase II project will greatly alleviate these shortcomings by providing lucrative, general approaches for phosphorylation analyses. The technologies will enable general phosphorylation detection, cost-effective cancer inhibitor screenings, and kinase/phosphatase activity quantitation for new drug discovery.

The broader impact/commercial potential of this project is the development of platform technology to improve a set of biochemical assays, thus enabling the discovery of new therapeutic targets and drugs. Protein phosphorylation and kinase inhibitors as drug targets are currently at the peak of research and development (R&D), responsible for over 30% of the total drug discovery expenses. These R&D activities could greatly benefit from the proposed technologies due to their innovative design and versatile features for optimum efficiency, and the ability to reproducibly explore phosphorylation events in unprecedented depth. These should provide invaluable tools and address needs of many bioscience research labs/facilities in academic and industrial settings.


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2015

DESCRIPTION provided by applicant With recent technical advances multiple important signaling pathways that may be the causes of human malignancy have continuously been discovered and dissected The vast majority of these signaling pathways involve reversible protein phosphorylation and the information on the location and dynamics of phosphorylation provides important mechanisms on how the signaling networks function and interact While mass spectrometry has become an exceptionally useful tool for phosphoproteome analyses extensive experiments are very labor intensive and cost prohibitive to most researchers As a result despite many large scale phosphoproteomic studies the critical need for a routine and effective analysis of relevant phosphoproteins has not been addressed Through this NIH SBIR Phase I study we will develop a novel strategy for gel based phosphoproteome analysis called Difference Gel Electrophoresis of Phosphoproteome DiGEP and translate it into commercial products for simpler phosphorylation discovery assays The novel design will take advantage of the small molecule platform functionalized with titanium ions for selective binding to phosphoproteins and a UV based crosslinker to immobilize the reagent onto the bound phosphoprotein In addition two different but structurally similar fluorophores will be used to differentiate the phosphoproteome profiles of two samples ran on a single gel The strategy will allow quantitative measurements of phosphorylation between the two samples and will pin point which contrasted phosphoproteins should be further analyzed by mass spectrometry The proposed approach offers the promise of significant cost reduction and is fully compatible with gel systems and imaging software already developed for Difference Gel Electrophoresis DIGE PUBLIC HEALTH RELEVANCE Protein phosphorylation relates to the onset and development of many cancer types and a highly efficient technology for phosphorylation analysis is critical for cancer research This NIH SBIR will support an effort to develop an innovative technology into commercial products that equip researchers with powerful tools and new directions to combat the devastating disease


Tymora Analytical Operations, Llc | Entity website

Microarray detection Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling ...


Tymora Analytical Operations, Llc | Entity website

About Tymora Tymora Analytical Operationsprovides new research products and reagents to Research and Development (R&D) organizations within the life sciences market. Tymora has developed a set of highly effective technologies for the analysis of protein phosphorylation that relates to the onset of numerous diseases, most notably cancer ...

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