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Wan Y.,TuoHua Biological Technology Company | Ben L.,TuoHua Biological Technology Company | Guan Z.-Q.,Corning Inc. | Li C.,TuoHua Biological Technology Company | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Stem cells expansion technology in vitro is affected by the microenvironment of the culture system. So, to find an effective method is particularly important to promote cell adherent and growth. Objective: To compare the effects of different culture media on cell expansion. Methods: Human umbilical cord mesenchymal stem cells at a density of 5.0×104 cells/cm2 were inoculated into Corning® polystyrene culture dishes coated with or without poly-L-ornithine and Corning® CellBIND culture dishes. Cell adhesion and proliferation were observed, and expressions of cell adhesion proteins and cell markers were detected. Results and Conclusion: Cell adhesion was promoted when cells were cultured in Corning® CellBIND® Surface medium coated with poly-L-ornithine for 24 hours,and the cultured cells grew at the logarithmic phase. The cell proliferation was also enhanced, and the cells expressed cell adhesion protein but not the cell markers of CD73, CD90, CD105. Corning® CellBIND® Surface medium was superior to Corning® polystyrene culture medium in the improvement of cell adhesion and proliferation. Additionally, both of these two media showed no influence on cell phenotype. These findings indicate that Corning® CellBIND® Surface medium can promote cell adhesion and proliferation, but shows no effects on cyclin and cell phenotype. This medium coated with poly-L-ornithine can further accelerate cell adhesion and proliferation, and stably express cell phenotype of human umbilical cord mesenchymal stem cells. Source


Li Z.-G.,Siping Center Hospital | Ben L.,TuoHua Biological Technology Company | Han Y.,TuoHua Biological Technology Company | Bi W.-W.,TuoHua Biological Technology Company
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: The relationship between placing time after stem cell preparation and cell survival is the basis of safety and effectiveness for the clinical application. OBJECTIVE: To observe effects of different preservation solutions and different storage time on survival rate of umbilical cord-derived mesenchymal stem cells, and to provide important evidence for identifying effectiveness of stem cells. METHODS: Umbilical cord-derived mesenchymal stem cells were selected to prepare stem cell preparation, which was preserved in physiological saline, medium, medium+physiological saline, physiological saline containing epidermal growth factor, and medium containing low molecular heparin calcium suspension. Cold closet was selected for imitating cellular transport conditions. Samples were obtained at 0, 6, 12, 13, 24, 30, 36, 42 and 43 hours. Total cell number and cell survival rate were detected. RESULTS AND CONCLUSION: The difference in cell number and survival rate was not great within 24 hours in each group. Twenty-four hours later, total cell number and survival rate were better in the medium and medium containing low molecular heparin calcium groups than in the physiological saline containing epidermal growth factor, physiological saline, nnd medium+physiological saline groups. These findings suggest that nfter stem cell preparation, the cell survival rate can reach more than 90% within 24 hours under refriierated transport conditions. Nutritional ingredients and proper pH value of preservation solution can make the cell survival rate increased greatly. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved. Source

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