Yang Z.,Tumor Hospital of Yunnan Province |
Tan J.,Tumor Hospital of Yunnan Province |
Xu Y.,Kunming General Hospital of Chengdu Military Command |
Sun H.,Tumor Hospital of Yunnan Province |
And 6 more authors.
European Spine Journal | Year: 2012
Purpose To investigate the effect of treatment of multiple myeloma (MM)-associated spinal fracture with percutaneous vertebroplasty (PVP) and chemotherapy. Methods Patients with MM-associated spinal fracture were randomly divided into combined (PVP and chemotherapy) treatment group (n = 38) and single chemotherapy group (n = 38). For the combined treatment group, bone cement was injected into vertebral body via DSA guided-percutaneous puncture. M2 scheme was used for both groups. And a 5-year follow-up was conducted for the study. Results At the 1-year follow-up visits, PVP combined with chemotherapy achieved complete remission (CR) in six patients (15.8%); near complete remission (nCR) in ten patients (26.30%); partial remission (PR) in nine patients (23.7%); minimal response (MR) in three patients (7.9%); no change (NC) in four patients (10.5%), and disease progression (DP) in five patients (13.2%). Only chemotherapy alone resulted in 3 CR (7.9%); 8 nCR (26.30%); 19 PR (77.5%); 4 MR (17.5%); 4 NC (17.5%), and 2 DP (5.0%). While the overall response rate (ORR) in the combined treatment group (65.8%) and the single chemotherapy group (50.0%) were significantly different, their visual analog pain scales (3.01 ± 0.62 and 5.97 ± 0.40, respectively) and Karnofsky performance scores (89.4 ± 6.3 and 80.3 ± 7.2, respectively) were significantly improved after treatment (P = 0.032 and P = 0.002, respectively). And the ORR between the two groups were significantly different (P = 0.001). Conclusion Percutaneous vertebroplasty is a minimally invasive surgery for MM-associated pathologic fracture. PVP had the characteristics of minimal trauma, easy operation and less complication. PVP can achieve longterm analgesic effect, and enhance the spinal stability. © 2011 Springer-Verlag.
Qin H.,Kunming University of Science and Technology |
Li G.F.,Tumor Hospital of Yunnan Province |
Chen N.,Tumor Hospital of Yunnan Province |
Yang Y.L.,Kunming University of Science and Technology
Journal of the Brazilian Chemical Society | Year: 2012
A novel approach, cosurfactants ultrasonic-thermostatic-assisted cloud point extraction (CUS-CPE) combined with high performance liquid chromatography and ultraviolet detection (HPLC-UV) is developed for the analysis of glucocorticoids (beclometasone dipropionate (BD), hydrocortisone butyrate (HB) and nandrolone phenylpropionate (NPP)) in human urine samples. In this study, four different cloud point extraction (CPE) systems are discussed, including DC-193-nonanoic acid, DC-193-sodium sulfate, DC-193-lauric acid and the classic Triton X-100 sulfate systems. Among them, DC-193-sodium sulfate and the classic Triton X-100 sulfate systems has been studied in the past few years. Comparing with the first two systems, DC-193-nonanoic acid system had a lower cloud point (CP), little UV absorbance and it is less damaging to the column of three glucocorticoids in same surfactant concentration which was required for application as a pre-concentration process prior to HPLC. Phase diagrams were used to study the cavitation and mass transfer behaviors of the two phases on micelles of polyether type organic silicon surfactant, PEG-12 dimethicone (DC-193).The volumes of surfactant-rich phase obtained were very small (the enrichment factor (EF) was 35), which was much smaller and had a quick phase separating speed than that of Triton X-100 in the same surfactant concentration. Linearity was investigated from 1 to 350 ng mL-1. The limits of detection (LOD) thus estimated were 1.29 for BD, 2.67 for HB and 3.33 ng mL -1 for NPP, respectively. In proposed CPE step is rapid and effective to obtain recovery of three glucocorticoids higher than 85%, which is similar or better than literature reported data. The method was shown to be selective, linear, precise and reproducible and successfully applied for the analysis of glucocorticoids in human urine samples. © 2012 Sociedade Brasileira de Química.
Huo L.,The Open University of Hong Kong |
Huo L.,University of Houston |
Yao H.,The Open University of Hong Kong |
Yao H.,Chongqing Medical University |
And 6 more authors.
PLoS ONE | Year: 2010
Background: hTERTC27 is a 27 kDa C-terminal polypeptide of human telomerase reverse transcriptase that has previously been shown to reduce tumorigenicity of HeLa cells and suppress growth of xenografted glioblastoma in nude mice. Although ectopic expression of hTERTC27 upregulated genes that are involved in apoptosis, cell cycle, and immune response, the mechanism for hTERTC27-induced tumor suppression has not been completely elucidated. Since hTERT was identified as a universal tumor-associated antigen, we hypothesize that hTERTC27 inhibits tumor growth in vivo through activation of anti-tumor immune response. Methodology/Principal Finding: Immunocopetent C57BL/6 mice were used for mouse B16 melanoma model. Mice bearing B16 melanoma were administered rAAV-/rAdv viral cocktail expressing hTERTC27, and tumor growth was monitored after viral cocktail treatment. Blood and splenocytes were used to determine the level of cytokines and the activity of immune cells, respectively. B16 tumor growth was significantly inhibited by subcutaneous administration of a single dose of 1.5×1011 vg rAAV-hTERTC27 and 2.5×109 pfu rAdv-hTERTC27 viral cocktail (rAAV-/rAdv-hTERTC27). The population and cytotoxicity of NK cells in the mice were significantly augmented by rAAV-/rAdv-hTERTC27 treatment, and selective depletion of the NK cell population in mice by intraperitoneal injection of anti-GM1 antibody abrogated the growth suppression of melanoma induced by rAAV-/rAdv-hTERTC27 administration. Conclusion: Activation of NK cells by administration of rAAV-/rAdv-hTERTC27 is critical for growth suppression of melanoma in mouse model. © 2010 Huo et al.
Wu M.,CAS Kunming Institute of Zoology |
Wu M.,University of Chinese Academy of Sciences |
Wu M.,Zunyi Medical College |
Tu T.,CAS Kunming Institute of Zoology |
And 3 more authors.
BMC Cancer | Year: 2013
Background: To understand the carcinogenesis caused by accumulated genetic and epigenetic alterations and seek novel biomarkers for various cancers, studying differentially expressed genes between cancerous and normal tissues is crucial. In the study, two cDNA libraries of lung cancer were constructed and screened for identification of differentially expressed genes.Methods: Two cDNA libraries of differentially expressed genes were constructed using lung adenocarcinoma tissue and adjacent nonmalignant lung tissue by suppression subtractive hybridization. The data of the cDNA libraries were then analyzed and compared using bioinformatics analysis. Levels of mRNA and protein were measured by quantitative real-time polymerase chain reaction (q-RT-PCR) and western blot respectively, as well as expression and localization of proteins were determined by immunostaining. Gene functions were investigated using proliferation and migration assays after gene silencing and gene over-expression.Results: Two libraries of differentially expressed genes were obtained. The forward-subtracted library (FSL) and the reverse-subtracted library (RSL) contained 177 and 59 genes, respectively. Bioinformatic analysis demonstrated that these genes were involved in a wide range of cellular functions. The vast majority of these genes were newly identified to be abnormally expressed in lung cancer. In the first stage of the screening for 16 genes, we compared lung cancer tissues with their adjacent non-malignant tissues at the mRNA level, and found six genes (ERGIC3, DDR1, HSP90B1, SDC1, RPSA, and LPCAT1) from the FSL were significantly up-regulated while two genes (GPX3 and TIMP3) from the RSL were significantly down-regulated (P < 0.05). The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer. The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro.Conclusions: The two libraries of differentially expressed genes may provide the basis for new insights or clues for finding novel lung cancer-related genes; several genes were newly found in lung cancer with ERGIC3 seeming a novel lung cancer-related gene. ERGIC3 may play an active role in the development and progression of lung cancer. © 2013 Wu et al.; licensee BioMed Central Ltd.
Luo Y.-C.,Military General Hospital of Beijing PLA |
Zhang H.-T.,Military General Hospital of Beijing PLA |
Zhang H.-T.,Southern Medical University |
Cheng H.-Y.,Tumor Hospital of Yunnan Province |
And 4 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2010
In this study, we examined the phenotypic characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-derived MSCs) differentiated along an oligodendrocyte pathway. We induced human UCB-derived MSCs to form floating neurospheres, and these neurospheres were then induced to differentiate into oligodendrocyte progenitor-like cells using multiple induction factors. Differentiated UCB-derived MSCs showed morphologic characteristics of an oligodendrocyte phenotype. The expression of cell surface markers characteristic of oligodendrocyte progenitor cells or oligodendrocytes was determined by immunocytochemical staining. These results suggest that human UCB-derived MSCs can be induced to differentiate into cells with an oligodendrocyte phenotype and that these cells may have potential in the future cellular therapy of central neurological disorders. © 2010 The Society for In Vitro Biology.
Huang Y.-G.,Kunming Medical University |
Huang Y.-G.,University of Chinese Academy of Sciences |
Li Y.-F.,Tumor Hospital of Yunnan Province |
Wang L.-P.,Yan an Hospital of Kunming |
And 2 more authors.
Journal of Cancer Research and Therapeutics | Year: 2013
Background: Recent evidence has indicated that the trefoil factor family possesses pivotal roles in the progression of human cancer. Aberrant expression of trefoil factor 3 (TFF3) has been reported to correlate with an aggressive tumor phenotype. However, the clinical importance of TFF3 expression in colorectal carcinomas (CRCs) has rarely been addressed. Purpose: To investigate the putative role of TFF3 in colorectal carcinogenesis and progress, and to clarify whether TFF3 could be a serum marker for CRCs. Materials and Methods: Fifty-six CRCs were sequenced for TFF3 mutations; subsets of the primary tumors were subjected to real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry analyses and serum TFF3 was detected by enzyme-linked immunosorbent assay (ELISA) for patients with CRCs. Results: No variants were detected in the code area of TFF3; TFF3 mRNA is increased in CRCs but not up to statistic significance when compared with paired normal colonic mucosa; TFF3 staining by immunohistochemistry in primary CRCs showed that increased expression of TFF3 is associated with lymph node metastases(LNM), and no significant differences were found with respect to patient's sex, cancer cell differentiation and stage. Serum TFF3 is significantly elevated in patients with CRCs, especially CRCs with LNM. Conclusion: The results indicate that TFF3 point mutations seem to be a rare event in colorectal carcinogenesis; TFF3 expression may play a role in promoting lymph node metastases of CRCs and serum TFF3 may be a potential useful marker for patients with CRCs and their metastases.
Zhou L.,Tumor Hospital of Yunnan Province |
He M.,Tumor Hospital of Yunnan Province
Chinese-German Journal of Clinical Oncology | Year: 2011
The general chemotherapy for Peripheral T-cell lymphoma (PTCL) featuring an active invasion has less curative effect and worse prognosis in comparison with that for B-cell non-Hodgkin's lymphoma (NHL). Studies in recent years suggest that hematopoietic stem cell transplantation (HSCT) has better curative effect on the PTCL; however, it is significant to do more studies on some aspects such as the methodology, punctuality, preconditioning, and pretreatment intensity of the transplantation, which are crucial to the curative effect. © 2011 Springer-Verlag.
Zuozhang Y.,Tumor Hospital of Yunnan Province |
Lin X.,Tumor Hospital of Yunnan Province |
Hongpu S.,Tumor Hospital of Yunnan Province |
Yunnchao H.,Tumor Hospital of Yunnan Province |
And 4 more authors.
Diagnostic and Interventional Radiology | Year: 2011
Percutaneous vertebroplasty is a minimally invasive surgical technique for the treatment of spinal damage and pathological compression fractures. Iodine-125 (I-125) particles can inhibit the uncontrolled proliferation of tumor cells to achieve anti-tumor effects. We report treating a spinal cord compression and T5 metastatic lung cancer patient through percutaneous vertebroplasty and I-125 seed implantation. Three years of follow-up demonstrated that our surgical plan achieved a satisfactory clinical outcome with good postsurgical recovery of spinal column function and relieved the symptoms of spinal cord compression. © Turkish Society of Radiology 2011.
Xie L.,Tumor Hospital of Yunnan Province |
Shen L.-D.,Tumor Hospital of Yunnan Province |
Qing C.,Kunming Medical College |
Yang Z.-Z.,Tumor Hospital of Yunnan Province |
And 3 more authors.
Medical Oncology | Year: 2012
Vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in primary malignant gastric lymphoma were studied, and their correlation as well as its clinical significance was analyzed. Thirty-five patients diagnosed with primary malignant gastric lymphoma were enrolled in this study. VEGF expression in the tumor tissues was detected by immunohistochemistry. Microvessel density in tumors was counted with Weidner's method and compared with MVD in normal tissues 5 cm away from tumor site. Collected data were analyzed statistically. Our results showed that VEGF expression and MVD in tumor tissues were higher than those in normal tissues, and the difference between these two groups was significant (P < 0.01). As VEGF expression was elevated, MVD was also increased in tumor tissues. Statistical analysis revealed that VEGF expression was positively correlated with MVD (r = 0.392, P < 0.05). VEGF was highly expressed in primary malignant gastric lymphoma and positively correlated with MVD. These results strongly suggest that anti-angiogenesis therapy investigated in gastric lymphoma is a prospective clinical trial. © Springer Science+Business Media, LLC 2011.
PubMed | Tumor Hospital of Yunnan Province
Type: Journal Article | Journal: Medical oncology (Northwood, London, England) | Year: 2012
Vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in primary malignant gastric lymphoma were studied, and their correlation as well as its clinical significance was analyzed. Thirty-five patients diagnosed with primary malignant gastric lymphoma were enrolled in this study. VEGF expression in the tumor tissues was detected by immunohistochemistry. Microvessel density in tumors was counted with Weidners method and compared with MVD in normal tissues 5 cm away from tumor site. Collected data were analyzed statistically. Our results showed that VEGF expression and MVD in tumor tissues were higher than those in normal tissues, and the difference between these two groups was significant (P < 0.01). As VEGF expression was elevated, MVD was also increased in tumor tissues. Statistical analysis revealed that VEGF expression was positively correlated with MVD (r = 0.392, P < 0.05). VEGF was highly expressed in primary malignant gastric lymphoma and positively correlated with MVD. These results strongly suggest that anti-angiogenesis therapy investigated in gastric lymphoma is a prospective clinical trial.