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Wu M.,CAS Kunming Institute of Zoology | Wu M.,University of Chinese Academy of Sciences | Wu M.,Zunyi Medical College | Tu T.,CAS Kunming Institute of Zoology | And 3 more authors.
BMC Cancer | Year: 2013

Background: To understand the carcinogenesis caused by accumulated genetic and epigenetic alterations and seek novel biomarkers for various cancers, studying differentially expressed genes between cancerous and normal tissues is crucial. In the study, two cDNA libraries of lung cancer were constructed and screened for identification of differentially expressed genes.Methods: Two cDNA libraries of differentially expressed genes were constructed using lung adenocarcinoma tissue and adjacent nonmalignant lung tissue by suppression subtractive hybridization. The data of the cDNA libraries were then analyzed and compared using bioinformatics analysis. Levels of mRNA and protein were measured by quantitative real-time polymerase chain reaction (q-RT-PCR) and western blot respectively, as well as expression and localization of proteins were determined by immunostaining. Gene functions were investigated using proliferation and migration assays after gene silencing and gene over-expression.Results: Two libraries of differentially expressed genes were obtained. The forward-subtracted library (FSL) and the reverse-subtracted library (RSL) contained 177 and 59 genes, respectively. Bioinformatic analysis demonstrated that these genes were involved in a wide range of cellular functions. The vast majority of these genes were newly identified to be abnormally expressed in lung cancer. In the first stage of the screening for 16 genes, we compared lung cancer tissues with their adjacent non-malignant tissues at the mRNA level, and found six genes (ERGIC3, DDR1, HSP90B1, SDC1, RPSA, and LPCAT1) from the FSL were significantly up-regulated while two genes (GPX3 and TIMP3) from the RSL were significantly down-regulated (P < 0.05). The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer. The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro.Conclusions: The two libraries of differentially expressed genes may provide the basis for new insights or clues for finding novel lung cancer-related genes; several genes were newly found in lung cancer with ERGIC3 seeming a novel lung cancer-related gene. ERGIC3 may play an active role in the development and progression of lung cancer. © 2013 Wu et al.; licensee BioMed Central Ltd.


Luo Y.-C.,Military General Hospital of Beijing PLA | Zhang H.-T.,Military General Hospital of Beijing PLA | Zhang H.-T.,Southern Medical University | Cheng H.-Y.,Tumor Hospital of Yunnan Province | And 4 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2010

In this study, we examined the phenotypic characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-derived MSCs) differentiated along an oligodendrocyte pathway. We induced human UCB-derived MSCs to form floating neurospheres, and these neurospheres were then induced to differentiate into oligodendrocyte progenitor-like cells using multiple induction factors. Differentiated UCB-derived MSCs showed morphologic characteristics of an oligodendrocyte phenotype. The expression of cell surface markers characteristic of oligodendrocyte progenitor cells or oligodendrocytes was determined by immunocytochemical staining. These results suggest that human UCB-derived MSCs can be induced to differentiate into cells with an oligodendrocyte phenotype and that these cells may have potential in the future cellular therapy of central neurological disorders. © 2010 The Society for In Vitro Biology.


Qin H.,Kunming University of Science and Technology | Li G.F.,Tumor Hospital of Yunnan Province | Chen N.,Tumor Hospital of Yunnan Province | Yang Y.L.,Kunming University of Science and Technology
Journal of the Brazilian Chemical Society | Year: 2012

A novel approach, cosurfactants ultrasonic-thermostatic-assisted cloud point extraction (CUS-CPE) combined with high performance liquid chromatography and ultraviolet detection (HPLC-UV) is developed for the analysis of glucocorticoids (beclometasone dipropionate (BD), hydrocortisone butyrate (HB) and nandrolone phenylpropionate (NPP)) in human urine samples. In this study, four different cloud point extraction (CPE) systems are discussed, including DC-193-nonanoic acid, DC-193-sodium sulfate, DC-193-lauric acid and the classic Triton X-100 sulfate systems. Among them, DC-193-sodium sulfate and the classic Triton X-100 sulfate systems has been studied in the past few years. Comparing with the first two systems, DC-193-nonanoic acid system had a lower cloud point (CP), little UV absorbance and it is less damaging to the column of three glucocorticoids in same surfactant concentration which was required for application as a pre-concentration process prior to HPLC. Phase diagrams were used to study the cavitation and mass transfer behaviors of the two phases on micelles of polyether type organic silicon surfactant, PEG-12 dimethicone (DC-193).The volumes of surfactant-rich phase obtained were very small (the enrichment factor (EF) was 35), which was much smaller and had a quick phase separating speed than that of Triton X-100 in the same surfactant concentration. Linearity was investigated from 1 to 350 ng mL-1. The limits of detection (LOD) thus estimated were 1.29 for BD, 2.67 for HB and 3.33 ng mL -1 for NPP, respectively. In proposed CPE step is rapid and effective to obtain recovery of three glucocorticoids higher than 85%, which is similar or better than literature reported data. The method was shown to be selective, linear, precise and reproducible and successfully applied for the analysis of glucocorticoids in human urine samples. © 2012 Sociedade Brasileira de Química.


Meng Q.,Kunming Medical University | Wan C.-H.,Guangdong Medical College | Luo J.-H.,Kunming Medical University | Tang X.-L.,Tumor Hospital of Yunnan Province | Cun Y.-L.,Tumor Hospital of Yunnan Province
Tumor | Year: 2011

Objective: To analyze the structure, reliability, validity and responsiveness of the six scales in the system of instruments measuring quality of life for patients with cancer (QLICP), which were developed to assess the quality of life for the patients with lung cancer, breast cancer, head and neck cancer, stomach cancer, colorectal cancer and cervical cancer. Methods: By using a stylized method, six QLICP-scales for lung cancer, breast cancer, head & neck cancer, stomach cancer, colorectal cancer and cervical cancer were developed. The quality of life of 692 patients with different tumors including lung cancer (n=85), breast cancer (n=186), head & neck cancer (n=133), stomach cancer (n=86), colorectal cancer (n=110) and cervical cancer (n=92) were measured. The reliability, validity and the responsiveness of the six scales were evaluated by calculating the Cronbach's α coefficient for the overall and each domain of the scale, calculating the test-retest reliability coefficient, and using exploratory factor analysis and the paired t-test. Results: All of the six scales in the system of QLICP have good structure and satisfactory psychometrics characteristics, which means that they have good reliability, validity and the tolerable responsiveness. Conclusion: The six special modules in the system of QLICP, including lung cancer, breast cancer, head and neck cancer, stomach cancer, colorectal cancer and cervical cancer modules, can be used in combination with general module of QLICP as the instrument to assess the quality of life for patients with different cancers. Copyright© 2011 by the Editorial Board of Tumor.

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