Tumor Hospital of Shandong Province

Jinan, China

Tumor Hospital of Shandong Province

Jinan, China

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Chen W.,Linyi Tumor Hospital | Liu J.,Linyi Tumor Hospital | Zhao L.,Linyi Traditional Chinese Medicine Hospital | Wang C.,Linyi Peoples Hospital | And 2 more authors.
Genetic Testing and Molecular Biomarkers | Year: 2016

Aims: To explore the associations between two endoplasmic reticulum (ER) stress proteins, protein disulfide isomerase (PDI), binding immunoglobulin protein (BIP), and the development and progression of pressure ulcers (PUs) in spinal cord injury (SCI) paraplegia patients. Methods: ELISA kits were used to measure the levels of serum PDI and BIP in 67 SCI paraplegia patients with PUs and 61 SCI paraplegia patients without PUs. The associations between PDI and BIP, PU formation, PU staging, and pressure ulcer scale for healing (PUSH) score were analyzed. Results: The patients in the PU group had higher levels of PDI and BIP than those in the non-PU group (both p < 0.05). Furthermore, the levels of PDI were positively correlated with those of BIP (r = 0.707, p < 0.0001). There were significant differences in the PDI and BIP levels among the different stages of PU (all p < 0.05). As the PU stages progressed, the levels of PDI and BIP first increased, then decreased, and finally peaked at stage III of the PUs. The PUSH scores significantly declined 7 days after debridement for the PU stage II (p < 0.01) but showed no significant difference between stages III and IV at 7 days after debridement (p > 0.05). The PUSH scores also decreased at 28 days after debridement for stages II, III, and IV (all p < 0.01). Higher PUSH scores indicated a longer time of debridement accompanied by a longer wound surface healing time (p < 0.05). Conclusion: ER stress proteins may be involved in the process of PU formation and healing; moreover, the levels of PDI and BIP were also associated with the severity of the PUs. Finally, we found that the PUSH scores can be used as a reference to evaluate PU severity and healing. © Copyright 2016, Mary Ann Liebert, Inc.


PubMed | Linyi Tumor Hospital, Linyi Peoples Hospital, Linyi Traditional Chinese Medicine Hospital and Tumor Hospital of Shandong Province
Type: Journal Article | Journal: Genetic testing and molecular biomarkers | Year: 2016

To explore the associations between two endoplasmic reticulum (ER) stress proteins, protein disulfide isomerase (PDI), binding immunoglobulin protein (BIP), and the development and progression of pressure ulcers (PUs) in spinal cord injury (SCI) paraplegia patients.ELISA kits were used to measure the levels of serum PDI and BIP in 67 SCI paraplegia patients with PUs and 61 SCI paraplegia patients without PUs. The associations between PDI and BIP, PU formation, PU staging, and pressure ulcer scale for healing (PUSH) score were analyzed.The patients in the PU group had higher levels of PDI and BIP than those in the non-PU group (both p<0.05). Furthermore, the levels of PDI were positively correlated with those of BIP (r=0.707, p<0.0001). There were significant differences in the PDI and BIP levels among the different stages of PU (all p<0.05). As the PU stages progressed, the levels of PDI and BIP first increased, then decreased, and finally peaked at stage III of the PUs. The PUSH scores significantly declined 7 days after debridement for the PU stage II (p<0.01) but showed no significant difference between stages III and IV at 7 days after debridement (p>0.05). The PUSH scores also decreased at 28 days after debridement for stages II, III, and IV (all p<0.01). Higher PUSH scores indicated a longer time of debridement accompanied by a longer wound surface healing time (p<0.05).ER stress proteins may be involved in the process of PU formation and healing; moreover, the levels of PDI and BIP were also associated with the severity of the PUs. Finally, we found that the PUSH scores can be used as a reference to evaluate PU severity and healing.


Sui J.-P.,Tumor Hospital of Shandong Province | Sui J.-P.,University of Jinan | Sui J.-P.,Jining Medical University | Ge B.-R.,Shandong Jining No1 Peoples Hospital | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: The abnormality of some parameters such as congruence angle has statistical significance on anterior knee pain has been confirmed under CT measurement, but has not been confirmed through clinical operations. Objective: To analyzes the relationship between the parameter of knee joint and anterior knee pain through measuring the parameters such as congruence angle, patellofemoral index, lateral patellofemoral angle and tilt angle. Methods: Fifty patients with anterior knee pain caused by varying degrees of atellofemoral cartilage damage were included, and the parameters such as congruence angle, patellofemoral index, lateral patellofemoral angle and tilt angle were measured on the patellar X-ray axial film. The parameters were compared with those of the other 50 patients without anterior knee pain and only with meniscus injury. Results and Conclusion: There were significant differences in congruence angle, patellofemoral index and tilt angle on the patellar X-ray axial film, and there was no significant difference in lateral patellofemoral angle. The congruence angle and patellofemoral index have important diagnosis value for the patients with anterior knee pain caused by patellofemoral joint disorders and are considered as the most effective parameters which has clinical efficacy for the patients with patellofemoral joint disorders.


Zhai G.,Tumor Hospital of Shandong Province | Zhai G.,Central Hospital of Zibo | Li G.,Shandong University | Xu B.,Central Hospital of Zibo | And 4 more authors.
Bioscience Reports | Year: 2016

Synopsis Radioresistance represents a major obstacle in cancer treatment, the underlying mechanism of which is complex and not well understood. miR-148b has been reported to be implicated regulating radioresistance in lymphoma cells. However, this function has not been investigated in lung cancer cells. Microarray analysis was performed in A549 cells 48 h after exposure to 8 Gy of γ-irradiation or sham irradiation to identify differentially expressed miRNAs. miR-148b mimic and inhibitor were transfected, followed by clonogenic survival assay to examine response to irradiation in A549 cells. Western Blot and luciferase assay were performed to investigate the direct target of miR-148b. Xenograft mouse models were used to examine in vivo function of miR-148b. Our data showed that expression of miR-148b was significantly down-regulated in both serum and cancerous tissues of radioresistant lung cancer patients compared with radiosensitive patients. Overexpression of miR-148b reversed radioresistance in A549 cells. MutL homologue 1 (MLH1) is the direct target of miR-148b which is required for the regulatory role of miR-148b in radioresistance. miR-148b mimic sensitized A549 xenografts to irradiation in vivo. Our study demonstrated that miR-148b regulates radioresistance of lung cancer cells by modulating MLH1 expression level. miR-148b may represent a new therapeutic target for the intervention of lung cancer. © 2016 Authors. This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0.


Wang Z.,Tumor Hospital of Shandong Province
International Journal of Clinical and Experimental Medicine | Year: 2014

Nowadays, advanced non-small cell lung cancer is still an incurable disease. Recent researches have led to considerable progress in the treatment of non-small cell lung cancer. This article reviews the main studies on chemotherapy on non-small cell lung cancer and discusses the new therapeutic strategies available to date. Stable disease (SD) is necessary in chemotherapy for tumor. The proportion of population with responders or SD basically maintained similar regardless of regimens. The overall survival after chemotherapy for patients with SD was lower than patients with responders, and higher than patients with progressive disease. Greater benefits could be achieved in patients with effective induction chemotherapy using chemotherapeutic agents for maintenance therapy, whereas the benefits were relatively small for patients with SD. It has been found that epidermal growth factor receptor (EGFR) mutation status had certain correlation with the efficacy of chemotherapy. First-line chemotherapy has shown advantages in effective rate and progression free survival on EGFR mutant. EGFR mutation produced significant effects on the efficacy of postoperative adjuvant chemotherapy. Patients with EGFR mutation had a higher effective rate than wild-type EGFR patients, and patients with responders had a greater benefit in progression free survival from maintenance therapy. However, it is still necessary to carry out more careful and deeper studies and analyses on traditional cytotoxic chemotherapy, to further optimize cytotoxic chemotherapy and to use molecular targeted agents with different mechanisms. © 2014, Int J Clin Exp Med. All Right Reserved.


Li M.,Tumor Hospital of Shandong Province | Yu J.,Tumor Hospital of Shandong Province
Chinese Journal of Clinical Oncology | Year: 2012

Kanglaite injection is a new, broad-spectrum, antitumor diphase emulsion, which can be intravenously administered. In recent years, studies have demonstrated that the antitumor activities of Kanglaite include the redistribution of tumor cell cycle, apoptosis induction in tumor cells, tumor angiogenesis and tumor growth inhibition, and COX-2 expression downregulation. In addition, the treatment can prolong the repair time of radiation injuries. Moreover, Kanglaite injection improved the patients' immunity and their quality of life when combined with radiotherapy for the treatment of lung cancer, nasopharyngeal cancer, esophageal cancer, and metastatic tumors.


Wang B.,Tumor Hospital of Shandong Province | Qiao L.,Capital Medical University | Shi Y.,Capital Medical University | Feng X.,Tumor Hospital of Shandong Province | And 2 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2015

OBJECTIVE: To investigate the role of apoptosis stimulating p53 binding protein 2 (ASPP2)-induced p53-dependent and p53-independent autophagy inhibition in apoptosis-promoting function of oxaliplatin (OXA).METHODS: According to different treatments, HCT116(p53(-/-)) cells were divided into 6 groups: rapamycin combined with ASPP2 group, ASPP2 group, p53 group, ASPP2 combined with p53 group, OXA combined with 3-methyladenine (3-MA) group, control group (OXA treatment or starvation without OXA treatment). When the level of apoptosis was detected, green fluorescent protein-advirus (GFP-Ad) group and rapamycin group were supplemented as controls. Cells were transfected with GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) plasmid, and LC3-expressing cells were calculated under a fluorescent microscope. Expressions of autophagy-related molecules were detected by Western blotting. Cells were subjected to annexin V-FITC/PI staining and apoptosis was assessed by flow cytometry.RESULTS: The 3-MA group showed the same inhibitory ability on autophagy with the ASPP2 group, and both of them were able to promote OXA or starvation-induced apoptosis, but the cell apoptosis rate in the 3-MA group was lower than that of the ASPP2 group. Rapamycin combined with ASPP2 still promoted OXA or starvation-induced apoptosis, and the apoptosis rate was also lower than that of the ASPP2 group. However, rapamycin counteracted effectively the inhibitory effect of ASPP2 on autophagy.CONCLUSION: OXA can induce autophagy of colorectal cancer cells, while ASPP2 over-expression can suppress the OXA-induced autophagy. ASPP2 can promote apoptosis through p53-dependent and p53-independent pathways. The function of ASPP2 promoting cell apoptosis through p53-dependent and p53-independent pathways is not entirely achieved by inhibiting cell autophagy.


Wang R.,Xinjiang Medical University | Wu R.,Xinjiang Medical University | Huang L.,Xinjiang Medical University | Yu J.,Tumor Hospital of Shandong Province
Chinese Journal of Clinical Oncology | Year: 2013

Objective: This study aims to investigate spontaneous cell apoptosis in the prediction of the radio-sensitivity of nasopharyngeal carcinoma (NPC) cells and the molecular foundation of differential spontaneous apoptosis rate. Methods: The surviving fractions and radiation biological parameters of well and poorly differentiated NPC cell lines (CNE-1/CNE-2) were determined through colony-forming assay and radiation dose-survival curve using a single-hit multi-target model and a linear quadratic model. Early- and late-stage apoptotic events were detected by flow cytometry on days 2, 4, 6, and 8. The mRNA and protein expression levels of apoptosis-correlated genes (Bcl-2, Bcl-xl, Bcl-w, Bax, Bad, Bid, and Bak) were detected using RT-PCR and western blot. Results: CNE-2 was more radio-sensitive than CNE-1, and CNE-2 exhibited higher rates of early- and late-stage apoptotic events than CNE-1 on the same culture days (P<0.05). The mRNA and protein expression levels of Bcl-2, Bcl-xl, Bcl-w, Bax, Bad, Bid, and Bak were significantly higher in CNE-2 than in CNE-1(P<0.05). Conclusion: The radio-sensitivity of NPC cells can be detected based on their spontaneous apoptosis rate, which is related to the different expression levels of apoptosis-correlated genes.


PubMed | Capital Medical University, Shandong University and Tumor Hospital of Shandong Province
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016

Tamoxifen (TAM) and fulvestrant (FUL) represent the major adjuvant therapy to estrogen receptor-alpha positive (ER(+)) breast cancer patients. However, endocrine resistance to TAM and FUL is a great impediment for successful treatment. We hypothesized that miR-21 might alter the sensitivity of breast cancer cells to TAM or FUL by regulating cell autophagy. Using the ER(+) breast cancer cells, we knockdown miR-21.by transfection with miR-21 inhibitor, then the cells were exposed to TAM or FUL and the percentages of apoptosis and autophagy were determined. Knockdown of miR-21 significantly increased the TAM or FUL-induced apoptosis in ER(+) breast cancer cells. Further, silencing of miR-21 in MCF-7 cells enhanced cell autophagy at both basal and TAM or FUL-induced level. The increase of autophagy in miR-21-knockdown MCF-7 cells was also indicated by increase of beclin-1, LC3-II and increased GFP-LC3 dots. Importantly, knockdown of miR-21 contributed to autophagic cell death, which is responsible for part of TAM induced cell death in miR-21 inhibitor-transfected cells. Further analysis suggested that miR-21 inhibitor enhance autophagic cell death through inhibition of PI3K-AKT-mTOR pathway. MiR-21 coordinated the function of autophagy and apoptosis by targeting Phosphatase and tensin homolog (PTEN) through inhibition of PI3K-AKT-mTOR pathway. In conclusion, silencing of miR-21 increased the sensitivity of ER(+) breast cancer cells to TAM or FUL by increasing autophagic cell death. Targeting autophagy-related miRNAs is a potential strategy for overcoming endocrine resistance to TAM and FUL.

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