Tumor Hospital of Shaanxi Province
Tumor Hospital of Shaanxi Province
Liu Y.-Z.,Tumor Hospital of Shaanxi Province |
Zhao X.-H.,First Affiliated Hospital Of Xian Jiaotong University |
Lv W.-D.,Tumor Hospital of Shaanxi Province |
Chang B.-L.,Tumor Hospital of Shaanxi Province
Chinese Journal of Tissue Engineering Research | Year: 2016
BACKGROUND: Apoptin is known to induce apoptosis of more than 50 kinds of tumor cells. However, efficient systems are required to deliver apoptin to the cancer cells for clinical use. OBJECTIVE: To construct human serum albumin (HSA) and Apoptin complex and transfect it to cancer cells in vitro and in vivo so as to find an efficient approach for apoptin delivery. METHODS: Polyethylenimine was used to react with N-hydroxysuccinimide (NHS)-HSA solution to synthesized HSA-PEI, and then react with pcDNA-Apoptin to construct recombinant HSA-PEI-pcDNA-Apoptin. HSA-PEI-pcDNA-Apoptin complex was transformed into MCF-7 breast cancer cell lines. Then the expression of apoptin was detected by western blot assay and the cell proliferation was detected by MTT assay and flow cytometry. The MCF-7 breast cancer cell xenograft model was used to detect the in vivo performance of HSA-PEI-pcDNA-Apoptin by measuring the tumor volume at 4 weeks, with saline and HSA-PEI-pcDNA as controls. RESULTS AND CONCLUSION: (1) We successfully prepared and confirmed the construction of HSA-PEI-pcDNA-Apoptin complex, which was successfully transformed into MCF-7 cells. (2) MCF-7 cells could express apoptin in the HSA-PEI-pcDNA-Apoptin group at 24 hours, but neither HSA-PEI-pcDNA group nor blank control group could express apoptin. (3) MTT assay for cell viability showed that HSA-PEI-pcDNA-Apoptin group had significantly lower optical density value than that in the other two groups (P < 0.05). (4) The tumor volume in the HSA-PEI-pcDNA-Apoptin group was significantly less than that in the other two groups (P < 0.05). (5) These findings indicate that the HSA-PEI-pcDNA-Apoptin complex markedly inhibits tumor growth in vitro and in vivo. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
PubMed | Xinjiang Medical University, The First Peoples Hospital of Shangqiu, Shantou University, Zhengzhou University and 43 more.
Type: Journal Article | Journal: Nature genetics | Year: 2014
We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) of esophageal squamous cell carcinoma (ESCC) in individuals of Chinese ancestry (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adjusted for age, sex, study and two eigenvectors, two new loci achieved genome-wide significance, marked by rs7447927 at 5q31.2 (per-allele odds ratio (OR) = 0.85, 95% confidence interval (CI) = 0.82-0.88; P = 7.72 10(-20)) and rs1642764 at 17p13.1 (per-allele OR = 0.88, 95% CI = 0.85-0.91; P = 3.10 10(-13)). rs7447927 is a synonymous SNP in TMEM173, and rs1642764 is an intronic SNP in ATP1B2, near TP53. Furthermore, a locus in the HLA class II region at 6p21.32 (rs35597309) achieved genome-wide significance in the two populations at highest risk for ESSC (OR = 1.33, 95% CI = 1.22-1.46; P = 1.99 10(-10)). Our joint analysis identifies new ESCC susceptibility loci overall as well as a new locus unique to the population in the Taihang Mountain region at high risk of ESCC.
Guo L.,Xi'an Jiaotong University |
Liu Z.-G.,Xi'an Jiaotong University |
Han P.-H.,Tumor Hospital of Shaanxi Province |
Yuan Q.,Xi'an Jiaotong University |
And 2 more authors.
Acta Radiologica | Year: 2012
Background: Imaging the perfusion of contrast media in breast tumors may allow improved diagnosing and treating breast cancer. Purpose: To compare the perfusion curve f (t) characteristics of contrast-enhanced ultrasonography in benign and malignant breast tumors. Material and Methods: Patients with breast tumors (n = 87) were evaluated with contrast-enhanced ultrasonography and the perfusion curve f (t) parameters were calculated using Sonoliverw software to compare analysis (tumor) and reference (normal) tissue areas. Differences between breast and breast tumors were assessed. Results: Compared to benign tumors, malignant tumors had faster enhancement time and a shorter mean transit time (all P values , 0.05). The intensity of the signal was also greater for malignant compared with benign tumors. Conclusion: Perfusion curve f (t) parameter measurements can distinguish differences in vascular flow between malignant and benign breast tumors and may provide a new quantitative indicator of breast tumor.
Lu W.-D.,Central South University |
Lu W.-D.,Tumor Hospital of Shaanxi Province |
Zhang M.,Central South University |
Wu Z.-S.,Central South University |
And 4 more authors.
Biomaterials | Year: 2010
In this study, valved photooxidatively crosslinked acellular bovine jugular vein conduits (BJVCs) were implanted in young dogs to reconstruct the connections of pulmonary arteries and right ventricles, with acellular conduits used as controls. All acellular conduits had moderate to severe valvular dysfunction and were explanted at 1-month implantation (n = 5). Histological examination showed inflammatory cell infiltration and intimal hyperplasia in the walls, and severe inflammatory cell infiltration and thrombosis in the valves. The photooxidatively crosslinked acellular conduits were retrieved at 1-month (n = 5) and 6-month (n = 5) implantations respectively. These conduits had excellent valvular function at retrieval. Their walls and valves were still soft and smooth without calcification and hemangioma. Endothelialization in valves and luminal walls was unsatisfied at 1-month retrieval, and was improved at 6-month retrieval. Host cells infiltrated and migrated from outer layer to the middle layer, with tissue remolding and regeneration found in these recellular regions. Histological examination and tissue content assay demonstrated that degeneration and regeneration of collagens and glycosaminoglycans were comparable, but elastic fibers gradually degraded. Photooxidatively crosslinked acellular BJVCs resist calcification and thrombosis and have regeneration patterns, with excellent hemodynamic performance. © 2009 Elsevier Ltd. All rights reserved.
Wang J.,The Fifth Hospital of Xian City |
Zhang H.,Tumor Hospital of Shaanxi Province |
Wang G.-Q.,Tumor Hospital of Shaanxi Province |
Quan Y.,The Fifth Hospital of Xian City
International Journal of Immunogenetics | Year: 2016
Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterized by symmetric polyarthritis. This study was designed to investigate the associations between HLA-DRB1 gene polymorphisms and RA in the Chinese Han population of Shaanxi province, northwest of China. In total, we identified 31 high-resolution HLA-DRB1 alleles in 109 patients with RA. Compared with the controls, the AF of HLA-DRB1*04:05 (P=0.000, Pc=0.007, OR = 3.462, 95%CI: 1.749-6.852) was significantly higher in patients with RA. In addition, the patients with RA had higher allele frequency (AF) of the HLA-DRB1*04:03 (P=0.030, Pc=NS, OR=4.737, 95%CI: 1.012-22.180); DRB1*04:06 (P=0.018, Pc=NS, OR=5.288, 95%CI: 1.145-24.422) and the shared epitope (SE) alleles (P=0.004, Pc=NS, OR=2.166, 95%CI: 1.279-3.667), respectively. Moreover, positive possibilities of RF and anti-CCP were significant higher in SE-positive patients compared to SR-negative patients (OR=4.787, 95%CI: 1.101-20.824; OR=3.775, 95%CI: 1.106-12.876, respectively). Our results indicated that HLA-DRB1*04:05, *04:03, *04:06 and SE alleles might be susceptibility allele for RA in Chinese Han population of Shaanxi province, northwest of China. © 2016 John Wiley & Sons Ltd.
PubMed | Xi'an Jiaotong University and Tumor Hospital of Shaanxi Province
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2015
Paclitaxel can exert therapeutic effects by interacting with microtubules. Stathmin and -III-tubulin, which have impact on microtubule activity, are believed to be involved in the chemotherapy. The purpose of the present study was to evaluate the associations between stathmin and -III-tubulin expression and treatment response and survivals in patients with non-small cell lung cancer (NSCLC). Two hundred thirty-eight patients who were treated by platinum-based chemotherapy were enrolled in this study, among them, 111 patients also received paclitaxel treatment. Formalin-fixed and paraffin-embedded tumor tissues were collected for messenger RNA (mRNA) and protein detection. We assessed the associations of the two molecules with treatment response and survival outcome. High level of stathmin exhibited poor response to chemotherapy (for mRNA, P=0.041; for protein, P=0.017). Overexpression of stathmin was associated with shorter overall survival (for mRNA, P=0.012; for protein, P=0.014) and progression-free survival (for mRNA, P=0.039; for protein, P=0.022). Of note, this association was only observed in patients who were treated by both platinum and paclitaxel. Similar effects were not observed for -III-tubulin. The findings demonstrated that paclitaxel effect may be interfered with stathmin; overexpression of stathmin is a predictive marker for a worse prognosis in patients with NSCLC who were treated by both platinum and paclitaxel chemotherapy.
PubMed | Northwest University, China, Xi'an Jiaotong University and Tumor Hospital of Shaanxi Province
Type: Journal Article | Journal: Journal of human genetics | Year: 2016
Genome-wide association studies (GWAS) have identified common variants associated with breast cancer (BC) risk at multiple genetic loci. Above all, accumulated evidence suggests that inherited risk variants may vary in BC subtypes defined by estrogen receptor (ER) or progesterone receptor (PR) status. However, the underlying susceptibility of some variants for BC subtypes has not been well investigated in the Chinese population. Our objective was to explore the association among 23 GWAS-identified single-nucleotide polymorphisms (SNPs) and overall BC incidence, as well as its subtypes, in Chinese women. An extensive association analysis using the Sequenom MassARRAY
PubMed | Tumor Hospital of Shaanxi Province, Northwest University, China, Inner Mongolia University and Traditional Chinese Medicine Hospital of Xian
Type: Journal Article | Journal: Oncotarget | Year: 2016
We performed a case-control study to investigate the associations between seven single nucleotide polymorphisms (SNPs) in the acylphosphatase 2 (ACYP2) gene and breast cancer (BC) risk in a Han Chinese population. There were 183 BC cases and 195 healthy controls included in the study. The SNPs were genotyped using the Sequenom MassARRAY platform. Logistic regression (adjusted for age group, body mass index [BMI], and menopause status), was used to evaluate the associations between the various genotypes and BC risk. Statistical analysis revealed that rs12621038 was associated with a decreased risk of BC in the allele (T vs. C: odds ratio [OR] = 0.71, 95% confidence interval [95% CI] = 0.52-0.94; p = 0.016), homozygous (TT vs. CC: OR = 0.47, 95% CI = 0.24-0.85; p = 0.014), dominant (OR = 0.62; 95% CI = 0.40-0.96; p = 0.032), and additive (OR = 0.68; 95% CI = 0.50-0.92; p = 0.012) models. In addition, we found that rs1682111 and rs17045754 were associated with the risk of BC and correlated with recurrence, and that rs6713088 correlated with tumor size. In sum, our findings reveal significant associations between SNPs in the ACYP2 gene and BC risk in a Han Chinese population.
PubMed | Yulin First Peoples Hospital, Xi'an Jiaotong University and Tumor Hospital of Shaanxi Province
Type: Journal Article | Journal: Neural regeneration research | Year: 2015
In the present study, a rat model of non-traumatic intracerebral hemorrhage was established by type IV collagenase injection into the right globus pallidus. Bax and Bcl-2 expression in tissues surrounding hematomas was significantly increased within 14 days after injury, and it then gradually decreased. Vascular endothelial growth factor, Flk-1 and Flt-1 mRNA expression gradually increased over time. After intraperitoneal injection with minocycline, Bax expression was decreased 1 day after intracerebral hemorrhage. Flk-1 and Flt-1 mRNA expression was decreased after minocycline injection, but Bcl-2 expression was increased, and vascular endothelial growth factor mRNA expression was decreased between 4-14 days. These results indicated that protective effects of minocycline on nerve tissues were associated with increased Bcl-2 expression and decreased Bax expression in the early stage after intracerebral hemorrhage. In the late stage, minocycline downregulated vascular endothelial growth factor and its receptor expression to inhibit brain tissue self-repair.
PubMed | Tumor Hospital of Shaanxi Province and The Fifth Hospital of Xian City
Type: Journal Article | Journal: International journal of immunogenetics | Year: 2016
Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterized by symmetric polyarthritis. This study was designed to investigate the associations between HLA-DRB1 gene polymorphisms and RA in the Chinese Han population of Shaanxi province, northwest of China. In total, we identified 31 high-resolution HLA-DRB1 alleles in 109 patients with RA. Compared with the controls, the AF of HLA-DRB1*04:05 (P=0.000, Pc=0.007, OR = 3.462, 95%CI: 1.749-6.852) was significantly higher in patients with RA. In addition, the patients with RA had higher allele frequency (AF) of the HLA-DRB1*04:03 (P=0.030, Pc=NS, OR=4.737, 95%CI: 1.012-22.180); DRB1*04:06 (P=0.018, Pc=NS, OR=5.288, 95%CI: 1.145-24.422) and the shared epitope (SE) alleles (P=0.004, Pc=NS, OR=2.166, 95%CI: 1.279-3.667), respectively. Moreover, positive possibilities of RF and anti-CCP were significant higher in SE-positive patients compared to SR-negative patients (OR=4.787, 95%CI: 1.101-20.824; OR=3.775, 95%CI: 1.106-12.876, respectively). Our results indicated that HLA-DRB1*04:05, *04:03, *04:06 and SE alleles might be susceptibility allele for RA in Chinese Han population of Shaanxi province, northwest of China.