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Shinagawa-ku, Japan

Ito F.,Tokyo Metroplitan University | Takahashi T.,TTI Ellebeau Inc. | Kanamura K.,Tokyo Metroplitan University | Kawakami H.,Tokyo Metroplitan University
Drug Development and Industrial Pharmacy | Year: 2013

The optimized preparation of Poly-(lactide-co-glycolic acid) (PLGA) nanospheres containing ubiquinone (UQ) for cosmetic products was pursued. By investigating various conditions for the preparation of UQ/PLGA nanospheres such as the molecular weight of PLGA, PLGA concentration, and UQ concentration, UQ/PLGA nanospheres with increased stability and slower drug release at a higher drug loading efficiency were prepared. Permeation tests on the prepared nanospheres using iontophoresis via electric dermal administration on membrane filters (200 nm pore size) and hairless mouse skin samples were also carried out. After iontophoresis, the nanospheres choked the membrane filter and remained on the horny layer of the hairless mouse skin, even after washing. Therefore, the prepared UQ/PLGA nanospheres and the established iontophoresis technique with the PLGA nanospheres in the present study can be applied to the future development of cosmetics. © 2013 Informa Healthcare USA, Inc. Source


Hama S.,Kyoto Pharmaceutical University | Kimura Y.,Kyoto Pharmaceutical University | Mikami A.,Kyoto Pharmaceutical University | Shiota K.,Kyoto Pharmaceutical University | And 6 more authors.
Journal of Biological Chemistry | Year: 2014

Background: Although skin is a tight barrier, transdermal liposome delivery is achievable by faint electric stimulus (ES). Results: ES caused rigid nanoparticle penetration into the epidermis, and induced connexin 43 phosphorylation, actin fiber depolymerization and Ca2 -influx. Conclusion: Our data indicate that ES opens epidermis intercellular spaces via intracellular signaling activation. Significance: Skin barrier permeability could be controlled by ES via changes in cutaneous physiological properties. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Source


Haida H.,Tokyo Medical and Dental University | Ando S.,Saitama Social Welfare Agency Asaka Kouyouen Dental Clinic for Disability | Ogami S.,Aichi University | Wakita R.,Tokyo Medical and Dental University | And 7 more authors.
Journal of Medical and Dental Sciences | Year: 2012

Calcium alginate gel has some unique properties, such as the capability to keep the drugs, bioadhesiveness, safety, and low cost. The purpose of this study is to determine whether calcium alginate gel can be used as a matrix of electrodes for iontophoresis (IOP). We measured the concentration of lidocaine transported from calcium alginate gels with various concentrations of alginic acid using an in vitro experimental cell with square-wave alternating current (AC) application. Temperature and pH changes were also determined during AC-IOP. The results revealed that lidocaine was released from calcium alginate gels at concentrations nearly 1.71-fold larger at 5 V, 60 min after AC application than in the case of passive diffusion. Lidocaine transport depended on the alginic acid concentration in the gels. Although there were slight increases in temperature and pH, chemical and thermal burns were not severe enough to be a concern. In conclusion, the calcium alginate gel can be used as a possible matrix for IOP electrodes. Source


Trademark
Tti Ellebeau Inc. and Transcutaneous Technologies Inc. | Date: 2006-06-30

compositions and chemicals used in association with medical devices to facilitate the non-invasive delivery of medication.


Yamamoto R.,TTI Ellebeau Inc. | Yamamoto R.,Hoshi University | Takasuga S.,TTI Ellebeau Inc. | Kominami K.,TTI Ellebeau Inc. | And 4 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2013

The aim of the present study was to evaluate the feasibility of transdermal delivery of glycyrrhizin, an agent used in the treatment of chronic hepatitis C, by cathodal iontophoresis using Ag/AgCl electrodes in vitro. The effects of donor pH (pH 4-7), concentration of drug (0.025-0.2% (w/v)), concentration of external chloride ions (Cl-) (0-133 mM), current strength (0-0.5 mA/cm2), and permeation enhancers (urea and Tween 80) on the skin permeability of glycyrrhizin were examined in in vitro skin permeation studies using porcine ear skin as the membrane. The cumulative amount of permeated glycyrrhizin and the steady-state skin permeation flux of glycyrrhizin across porcine skin increased in a pH-dependent manner. The skin permeability of glycyrrhizin was independent of the concentration of drug and competed only with a high external Cl-concentration. The skin permeation flux of glycyrrhizin increased with the current (R2=0.8955). The combination of iontophoresis and enhancers provided an additive or synergistic effect, and a skin permeation flux of about 60 μg/ h/cm2 was achieved. The plasma concentration of glycyrrhizin in humans, extrapolated from the in vitro steady-state permeation flux across porcine skin, was within the therapeutic level. These results suggest that cathodal iontophoresis can be used as a transdermal drug delivery system for glycyrrhizin using reasonable patch sizes and acceptable levels of current intensity. © 2013 The Pharmaceutical Society of Japan. Source

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