Tsuno Food Industrial Co.

Wakayama-shi, Japan

Tsuno Food Industrial Co.

Wakayama-shi, Japan
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Fukumoto K.,University of Fukui | Tsuno T.,Tsuno Food Industrial Co. | Taniguchi M.,Niigata University | Terada S.,University of Fukui
Cytotechnology | Year: 2013

As rice bran contains various nutrients and other proteins of which a part has biological effects on animal cells, we tested the effect of rice bran extract on rat mesenchymal stem cells (rMSCs) obtained from bone marrow. These rMSCs are pluripotent and can be readily induced to differentiate into a number of cell types, including bone and cartilage. rMSC was aggregated by culturing in serum-free condition with rice bran extract, but was not aggregated by culturing in serum-free condition or in serum-containing medium. Moreover, the longer aggregates of rMSCs were cultured in serum-free condition with rice bran extract, the more the aggregates grew. After two passages in serum-free conditions, rMSCs lost their potency for differentiation into osteogenic cells; however, the addition of rice bran extract to serum-free medium successfully prevented the loss of this ability for differentiation. In addition, MSC makers CD105 and CD166 gene expression in serum-free condition with rice barn extract corresponded to these expressions in serum-containing medium. This result suggests that certain factors in rice bran could be bioactive and contribute toward retaining the ability of MSCs to differentiate into osteogenic cells after passaging. © 2013 Springer Science+Business Media Dordrecht.


Mitsui R.,Okayama University of Science | Hirota M.,Okayama University of Science | Tsuno T.,Tsuno Food Industrial Co. | Tanaka M.,Okayama University of Science
FEMS Microbiology Letters | Year: 2010

Vanillin dehydrogenases (VDHs) were purified and characterized from two bacterial strains that have different pH dependencies for growth. The alkaliphile Micrococcus sp. TA1, isolated from an alkaline spa, can grow on several aromatic compounds such as ferulic acid, vanillin, vanillic acid, and protocatechuic acid under alkaline conditions. The neutrophile Burkholderia cepacia TM1, which was isolated previously, also grew on the above-mentioned compounds because they functioned as the sole carbon source under neutral conditions. Purified VDHs showed activities toward some aromatic aldehydes. These enzymes have the same subunit molecular mass of about 57 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but differed in some of their observed properties. Native molecular masses also differed between the purified enzymes. These were 250 kDa for the enzyme from alkaliphilic strain TA1 and 110 kDa for that from neutrophilic strain TM1, as determined by gel filtration. The enzyme from strain TA1 required NADP+ as a coenzyme for its activity, but that from strain TM1 required NAD+. These results are important because this is the first report of an alkaliphilic bacterium consuming lignin monomers. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.


Sakata K.,Japan National Institute of Advanced Industrial Science and Technology | Sakata K.,University of Tsukuba | Kawasaki H.,Japan National Institute of Advanced Industrial Science and Technology | Suzuki T.,Japan National Institute of Advanced Industrial Science and Technology | And 9 more authors.
Frontiers in Pharmacology | Year: 2015

Accumulating evidence indicates that the molecular circadian clock underlies the mating behavior of Drosophila melanogaster. However, information about which food components affect circadian mating behavior is scant. The ice plant, Mesembryanthemum crystallinum has recently become a popular functional food. Here, we showed that the close-proximity (CP) rhythm of D. melanogaster courtship behavior was damped under low-nutrient conditions, but significantly enhanced by feeding the flies with powdered ice plant. Among various components of ice plants, we found that myo-inositol increased the amplitude and slightly shortened the period of the CP rhythm. Real-time reporter assays showed that myo-inositol and D-pinitol shortened the period of the circadian reporter gene Per2-luc in NIH 3T3 cells. These data suggest that the ice plant is a useful functional food and that the ability of inositols to shorten rhythms is a general phenomenon in insects as well as mammals. © 2015 Sakata, Kawasaki, Suzuki, Ito, Negishi, Tsuno, Tsuno, Yamazaki and Ishida.


Endo Y.,Tokyo University of Technology | Hayashi C.,Tsuno Food Industrial Co. | Yamanaka T.,Tsuno Food Industrial Co. | Takayose K.,Tsuno Food Industrial Co. | And 3 more authors.
JAOCS, Journal of the American Oil Chemists' Society | Year: 2013

Acrolein, which is an irritating and off-flavor compound formed during heating of vegetable oils, was estimated by the gas-liquid chromatography (GLC). Several vegetable oils such as high-oleic sunflower, perilla, rapeseed, rice bran, and soybean oils were heated at 180 C for 480 min and then the concentration of acrolein in the head space gas was determined by GLC. The formation of acrolein was greatest in perilla oil among the tested oils, while it was much lower in rice bran oil and high-oleic sunflower oil. There was a good correlation between the level of acrolein and linolenate (18:3n-3) in the vegetable oils. To investigate the formation of acrolein from linolenate, methyl oleate, methyl linoleate, and methyl linolenate were also heated at 180 C, and the amounts of acrolein formed from them were determined by GLC. The level of acrolein was the greatest in methyl linolenate. Acrolein was also formed from methyl linoleate, but not from methyl oleate. Acrolein in vegetable oils may be formed from polyunsaturated fatty acids, especially linolenic acid but not from glycerol backbone in triacylglycerols. © 2013 AOCS.


PubMed | Tohoku University and Tsuno Food Industrial CO.
Type: Journal Article | Journal: Food & function | Year: 2016

Although the beneficial effects (e.g., lipid-lowering activity) of -oryzanol (OZ), a mixture of ferulic acid esters of plant sterols and triterpene alcohols, have been extensively investigated, few studies have evaluated the absorption and metabolism of OZ. Moreover, it is unclear whether OZ, once ingested, is directly absorbed by the intestine into the bloodstream at a sufficient level to exhibit activity. Here, we prepared OZ concentrate from purified rice bran oil (Rice Oil OZ), determined the concentration of OZ in the preparation (cycloartenyl ferulate equivalent concentration; 52.2%), and then carried out chromatography-mass spectrometry analysis of plasma samples from mice after oral administration of Rice Oil OZ. The OZ concentrations of plasma from the control (vehicle-treated) mice were low (trace levels); however, at 5 h after a single oral administration of the Rice Oil OZ (600 mg per kg body weight), the levels significantly increased, reaching 17.6 ng mL


Patent
Tsuno Food Industrial Co. and Kao Corporation | Date: 2011-08-19

A process for producing triterpene alcohol, comprising sequentially conducting the following steps (A) to (C):


Taiyoji M.,Niigata Agricultural Research Institute | Yamanaka T.,Tsuno Food Industrial Co. | Tsuno T.,Tsuno Food Industrial Co. | Ohtsubo S.,Niigata Agricultural Research Institute
Bioscience, Biotechnology and Biochemistry | Year: 2013

Arg-specific gingipain (Rgp) is a major pathogenic determinant of Porphyromonas gingivalis which is a major pathogen in periodontal disease. We prepared protein extracts with Rgp-inhibitory activity from polished rice (Oryza sativa) and evaluated the effects of these extracts on the growth and pathogenicity of P. gingivalis. The extracts inhibited the proteolytic degradation of human proteins by P. gingivalis proteinases, and repressed the growth and homotypic biofilm formation of P. gingivalis. The disruption of adhesion of epithelial cells by P. gingivalis was also restricted by the rice protein extracts. Our results suggested that the rice protein extracts suppressed the pathogenicity and growth of P. gingivalis by inhibiting the bacterial proteinase activities, implying that the Rgp-inhibitory proteins prepared from rice may be potentially valuable as nutraceutical agents for preventing periodontal diseases.


Kobayashi T.,Kyoto University | Ogino A.,Kyoto University | Miyake Y.,Industrial Technology Center Of Okayama Prefecture | Mori H.,Industrial Technology Center Of Okayama Prefecture | And 4 more authors.
Journal of the American Oil Chemists' Society | Year: 2014

Oleic acid esters of phytosterols (PSs) and triterpene alcohols (TAs), derived from rice bran, were synthesized using lipases under mild conditions. Some lipases, especially from Candida rugosa, type VII, showed very high substrate specificity towards both PSs and TAs, when a mixture of PS and TA (PS/TA mixture) was used as the substrate source. The maximum yield of PS esters was ca. 80 % in each case; however, the maximum yield of TA esters was much lower when the reaction was continued for 7 days. Due to the difficulty in purifying the esters obtained when the PS/TA mixture was used as source of substrate, free PSs and TAs were separated from the PS/TA mixture by silica-gel and reverse-phase chromatography prior to esterification. The pure PSs or TAs were esterified with oleic acid to obtain the corresponding esters with high purity. Differential scanning calorimetric analysis of the resulting esters revealed that their melting points ranged from 7.0 to 42 °C. These values were at least 100 °C lower than those of the free PSs and TAs. © 2014 AOCS.


Taniguchi H.,Industrial Technology Center Of Okayama Prefecture | Hashimoto H.,Tsuno Food Industrial Co. | Hosoda A.,Industrial Technology Center Of Okayama Prefecture | Kometani T.,Kinki University | And 2 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2012

Consuming rice as the staple, 0.95 million tons of rice bran is produced annually in Japan as a by-product from the rice milling process. Bran is used in several applications such as animal feed and fertilizer for mushroom cultivation, but most of it has been discarded as an agricultural waste although it contains various functional substances, such as γ-oryzanol, ferulic acid, sterol, wax, ceramide, phytin, inositol and protein. It could be considered a scarcely used but promising resource. As such, continuous efforts have been dedicated to exploring its effective utilization. In this context, functionalities of the substances contained in the bran are summarized, and our attempts for improving functionality, improving ease of use, and exploring new applications are presented.


Patent
Tsuno Food Industrial Co. | Date: 2013-09-18

The disclosed method for producing a -oryzanol-containing fat or oil, the method comprising the steps of:(A) preparing a processed fat or oil having an oil layer and a soapstock (alkali-foots) layer containing a -oryzanol salt; and(B) adding an acid to the processed fat or oil of step (A) for transfer of -oryzanol from the soapstock layer to the oil layer, and collecting a fat or oil with an increased -oryzanol content, enables inexpensive production of a fat or oil containing -oryzanol at any desired concentration without restriction to the -oryzanol concentration in the raw fat or oil.

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