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Fukumoto K.,University of Fukui | Tsuno T.,Tsuno Food Industrial Co. | Taniguchi M.,Niigata University | Terada S.,University of Fukui
Cytotechnology | Year: 2013

As rice bran contains various nutrients and other proteins of which a part has biological effects on animal cells, we tested the effect of rice bran extract on rat mesenchymal stem cells (rMSCs) obtained from bone marrow. These rMSCs are pluripotent and can be readily induced to differentiate into a number of cell types, including bone and cartilage. rMSC was aggregated by culturing in serum-free condition with rice bran extract, but was not aggregated by culturing in serum-free condition or in serum-containing medium. Moreover, the longer aggregates of rMSCs were cultured in serum-free condition with rice bran extract, the more the aggregates grew. After two passages in serum-free conditions, rMSCs lost their potency for differentiation into osteogenic cells; however, the addition of rice bran extract to serum-free medium successfully prevented the loss of this ability for differentiation. In addition, MSC makers CD105 and CD166 gene expression in serum-free condition with rice barn extract corresponded to these expressions in serum-containing medium. This result suggests that certain factors in rice bran could be bioactive and contribute toward retaining the ability of MSCs to differentiate into osteogenic cells after passaging. © 2013 Springer Science+Business Media Dordrecht.

Mitsui R.,Okayama University of Science | Hirota M.,Okayama University of Science | Tsuno T.,Tsuno Food Industrial Co. | Tanaka M.,Okayama University of Science
FEMS Microbiology Letters | Year: 2010

Vanillin dehydrogenases (VDHs) were purified and characterized from two bacterial strains that have different pH dependencies for growth. The alkaliphile Micrococcus sp. TA1, isolated from an alkaline spa, can grow on several aromatic compounds such as ferulic acid, vanillin, vanillic acid, and protocatechuic acid under alkaline conditions. The neutrophile Burkholderia cepacia TM1, which was isolated previously, also grew on the above-mentioned compounds because they functioned as the sole carbon source under neutral conditions. Purified VDHs showed activities toward some aromatic aldehydes. These enzymes have the same subunit molecular mass of about 57 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but differed in some of their observed properties. Native molecular masses also differed between the purified enzymes. These were 250 kDa for the enzyme from alkaliphilic strain TA1 and 110 kDa for that from neutrophilic strain TM1, as determined by gel filtration. The enzyme from strain TA1 required NADP+ as a coenzyme for its activity, but that from strain TM1 required NAD+. These results are important because this is the first report of an alkaliphilic bacterium consuming lignin monomers. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Tsuno Food Industrial Co. and Kao Corporation | Date: 2011-08-19

A process for producing triterpene alcohol, comprising sequentially conducting the following steps (A) to (C):

Sakata K.,Japan National Institute of Advanced Industrial Science and Technology | Sakata K.,University of Tsukuba | Kawasaki H.,Japan National Institute of Advanced Industrial Science and Technology | Suzuki T.,Japan National Institute of Advanced Industrial Science and Technology | And 9 more authors.
Frontiers in Pharmacology | Year: 2015

Accumulating evidence indicates that the molecular circadian clock underlies the mating behavior of Drosophila melanogaster. However, information about which food components affect circadian mating behavior is scant. The ice plant, Mesembryanthemum crystallinum has recently become a popular functional food. Here, we showed that the close-proximity (CP) rhythm of D. melanogaster courtship behavior was damped under low-nutrient conditions, but significantly enhanced by feeding the flies with powdered ice plant. Among various components of ice plants, we found that myo-inositol increased the amplitude and slightly shortened the period of the CP rhythm. Real-time reporter assays showed that myo-inositol and D-pinitol shortened the period of the circadian reporter gene Per2-luc in NIH 3T3 cells. These data suggest that the ice plant is a useful functional food and that the ability of inositols to shorten rhythms is a general phenomenon in insects as well as mammals. © 2015 Sakata, Kawasaki, Suzuki, Ito, Negishi, Tsuno, Tsuno, Yamazaki and Ishida.

Endo Y.,Tokyo University of Technology | Hayashi C.,Tsuno Food Industrial Co. | Yamanaka T.,Tsuno Food Industrial Co. | Takayose K.,Tsuno Food Industrial Co. | And 3 more authors.
JAOCS, Journal of the American Oil Chemists' Society | Year: 2013

Acrolein, which is an irritating and off-flavor compound formed during heating of vegetable oils, was estimated by the gas-liquid chromatography (GLC). Several vegetable oils such as high-oleic sunflower, perilla, rapeseed, rice bran, and soybean oils were heated at 180 C for 480 min and then the concentration of acrolein in the head space gas was determined by GLC. The formation of acrolein was greatest in perilla oil among the tested oils, while it was much lower in rice bran oil and high-oleic sunflower oil. There was a good correlation between the level of acrolein and linolenate (18:3n-3) in the vegetable oils. To investigate the formation of acrolein from linolenate, methyl oleate, methyl linoleate, and methyl linolenate were also heated at 180 C, and the amounts of acrolein formed from them were determined by GLC. The level of acrolein was the greatest in methyl linolenate. Acrolein was also formed from methyl linoleate, but not from methyl oleate. Acrolein in vegetable oils may be formed from polyunsaturated fatty acids, especially linolenic acid but not from glycerol backbone in triacylglycerols. © 2013 AOCS.

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