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Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Kulkarni S.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Chemical and Pharmaceutical Research | Year: 2011

A novel, safe, accurate, sensitive and economic Spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate and 8 M urea solution (50:50% V/V) as hydrotropic solubilizing agent for the quantitative determination of poorly water-soluble pramipexole dihydrochloride (solubility:- 1.4 × 10-01 mg/ml in water) in tablet dosage form. The solubility of Pramipexole Dihydrochloride increases more than 46 times in mixed hydrotropic solution as compared to solubility in distilled water. Pramipexole dihydrochloride shows maximum absorbance at 262 nm. Sodium acetate, urea and other tablets excipents did not show any absorbance above 250 nm and thus no interference in the estimation was seen. Pramipexole dihydrochloride was obeyed Lambert Beer's law in the concentration range of 15 to 75 μg/ml (r2= 0.9997) in mixed hydrotropic agent with mean recovery was found 98.35±0.55%. The percent concentration in marketed tablet formulation was found 97.52±1.65%. Parameters such as linearity, precision, accuracy, specificity and robustness were studied as reported in the International Conference on Harmonization guidelines. The relative standard deviations for three replicate measurements in five concentrations of samples were always less than 2%. So this method can successfully employ in the routine analysis of pramipexole dihydrochloride in bulk drug and tablet dosage forms.


Bhatnagar P.,Guru Ramdas Khalsa Institute of Science and Technology | Dhote V.,Truba Institute of Pharmacy | Mahajan S.C.,Mahakal Institute of Pharmaceutical Studies | Mishra D.K.,P.A. College
Current Drug Delivery | Year: 2014

The solubility of drugs is one of the most challenging aspects in developing formulations for novel drug discovery. Myriad of approaches have been developed and tested to overcome the associated intricacies involved with poor water soluble drugs. Out of the available technologies, solid dispersion (SD) method that significantly enhances the solubility and bioavailability by reducing particle size to a micro-molecular level is often viewed as a promising strategy. Although conceptual basis of manufacturing processes involved in SD method have been reported, formulation characteristics addressing solubility issues remains yet elusive. The current review portray the historical milestones, classification, probable mechanisms for enhancement of solubility, manufacturing processes at commercial level along with pioneer breakthroughs in field that enunciates the versatile pharmaceutical application for categories including anti-cancer and anti-retroviral drugs. Besides, our article also highlights the translational implications of drug development by SD method hitherto unreported. © 2014 Bentham Science Publishers.


Patel N.S.,Truba Institute of Pharmacy
Indian Journal of Pharmacology | Year: 2013

Objectives: The present study was undertaken to evaluate the radioprotective and cytoprotective potential of cordifolioside-A, a primary active constituent of n-butanol fraction of Tinospora Cordifolia (NBTC) against 4 Gy-γ radiation in mice and cyclophosphamide induced genotoxicity. Materials and Methods: Presence of cordifolioside-A in NBTC stem ethanolic extract was confirmed by high performance thin layer chromatography (HPTLC) analysis. Radioprotective activity was evaluated at 80 and 120 mg/kg, intraperitoneal (i.p.) dose of NBTC administered 15 days prior to whole body radiation exposure by observing survival rate, change in body weight, hematology, spleen colony forming unit (CFU), and micronucleus (MN) expression. Cytoprotective activity of NBTC was evaluated at 5, 10, and 15 mg/ml concentrations on Allium cepa root meristem growth against cyclophosphamide. Results: HPTLC analysis of standard cordifolioside A, and NBTC confirmed the presence of cordifolioside-A in NBTC with the retention factor value of 0.86. Administration of NBTC (120 mg/kg, i.p.) produced significant protection against radiation in terms of increased survival rate, body weight retention, hematological parameters, spleen CFU assay (P < 0.01), and decreased MN expression (P < 0.01). Cytoprotectivity was observed maximally at 10 mg/ml NBTC concentration with significant increase in root growth (P < 0.01), non-toxic mitotic index (MI) (65.9%) and lesser chromosomal aberrations (15.4%). NBTC at 10 mg/ml concentration showed very few C-anaphase compared to aberrations like fragmentation, C-anaphase, multipolarity and sticky chromosome in cyclophosphamide alone. Conclusion: The results suggest that enriched NBTC containing cordifolioside-A has a potential in vivo radioprotective effect as well as in vitro cytoprotective activity.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Chandel H.S.,Truba Institute of Pharmacy
Natural Product Research | Year: 2010

The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. calamus extract may have therapeutic benefits in wound healing. © 2010 Taylor & Francis.


Jain D.K.,Truba Institute of Pharmacy
Natural Product Research | Year: 2010

The aim of the present study was to assess the anti-fertility activity of ethanolic extracts of Tabernaemontana divaricata (TD) leaves in oestrogenic activity models in immature female rats. Mature green leaves of TD were collected and authenticated. Extractions of the dried leaves were carried out with ethanol in a Soxhlet's apparatus. For oestrogenic activity, the extracts were administered orally once daily at a dose of 200 and 400 mg kg-1, and the activity was compared with the standard drug ethinyl oestradiol (0.02 mg). The extracts caused significant increase in uterine weight compared to the control. The ethanolic extract exhibited oestrogenic activity. The histological study of epithelium tissues with the 400 mg of TD extract-treated animals showed increases in the height of the luminal epithelium and loose edematous stroma when compared with the 200 mg of TD extract-treated group of animals. However, this was better than the control group of animals. Enhanced uterine weight and increase in the height of luminal epithelium and histological characteristics suggest that TD extract may be useful in anti-fertility therapy. © 2010 Taylor & Francis.


PubMed | Dr Hari Singh Gour University, Truba Institute of Pharmacy and IPS Academy
Type: Journal Article | Journal: Drug delivery and translational research | Year: 2015

Solid dispersion has emerged as a method of choice and has been extensively investigated to ascertain the in vivo improved performance of many drug formulations. It generally involves dispersion of drug in amorphous particles (clusters) or in crystalline particles. Comparatively, in the last decade, amorphous drug-polymer solid dispersion has evolved into a platform technology for delivering poorly water-soluble small molecules. However, the success of this technique in the pharmaceutical industry mainly relies on different drug-polymer attributes like physico-chemical stability, bioavailability and manufacturability. The present review showcases the efficacy of amorphous solid dispersion technique in the research and evolution of different drug formulations particularly for those with poor water soluble properties. Apart from the numerous mechanisms of action involved, a comprehensive summary of different key parameters required for the solubility enhancement and their translational efficacy to clinics is also emphasized.


PubMed | Truba Institute of Pharmacy
Type: Journal Article | Journal: Indian journal of pharmacology | Year: 2016

The aim of this study was to investigate the antipsoriatic activity of ethanolic extract of For induction of psoriasis, 0.1 ml of prepared complete Freunds adjuvant (CFA) and formaldehyde mixture (1:10 ratio) was topically applied for 7 days on the dorsum surface of the skin of Swiss albino mice. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Therapeutic effect of 0.05% and 0.1% (w/w) ointments of EEWF was evaluated after the induction of psoriasis. Ointments of EEWF flowers were applied once daily for 3 weeks, and antipsoriatic activity was evaluated by scoring the PSI and histological examination.We observed the phenotypic and histological features and found a progressive reduction (The results showed that 0.05% and 0.1% (w/w) ointments of EEWF have dose-dependent beneficial effects in CFA and formaldehyde-induced psoriasis. The present investigation revealed that


Srivastava A.K.,Truba Institute of Pharmacy | Khare P.,Sri Aurobindo Institute of Pharmacy | Nagar H.K.,Truba Institute of Pharmacy | Raghuwanshi N.,Indian Institute of Technology Roorkee | Srivastava R.,Moradabad Educational Trust Group of Instituions
Current Protein and Peptide Science | Year: 2016

Hydroxyproline is a non-essential amino acid found in collagen and few other extracellular animal proteins. It’s two isomeric forms trans-4-hydroxy-L-proline and trans-3-hydroxy-L-proline play a crucial role in collagen synthesis and thermodynamic stability of the triple-helical conformation of collagen and associated tissues. Various abnormalities in hydroxyproline metabolism have been shown to play key roles in the pathophysiology and pathogenesis of different diseases. The elevated level of hydroxyproline is observed in several disorders, e.g., graft versus host disease, keloids, and vitiligo while its decreased level is a marker of poor wound-healing. This review explores the potential of using hydroxyproline as a biochemical marker to understand the pathogenesis, molecular pathophysiology and treatment of these diseases. The review concludes with an outlook on the scope and challenges in the clinical implementation of hydroxyproline as a biomarker. © 2016 Bentham Science Publishers.


PubMed | Truba Institute of Pharmacy
Type: | Journal: Current stem cell research & therapy | Year: 2016

Stem cell research is a rapidly developing field that offers effective treatment for a variety of malignant and non-malignant diseases. Stem cell is a regenerative medicine associated with the replacement, repair, and restoration of injured tissue. Stem cell research is a promising field having maximum therapeutic potential. Cancer stem cells (CSCs) are the cells within the tumor that posses capacity of self-renewal and have root cause for the failure of traditional therapies leading to re-occurrence of cancer. CSCs have been identified in blood, breast, brain, and colon cancer. Traditional therapies target only fast growing tumor mass but not slow-dividing cancer stem cells. It has been shown that embryonic pathways such as Wnt, Hedgehog and Notch, control self-renewal capacity and involved in cancer stem cells maintenance. Targeting of these pathways may be effective in eradicating cancer stem cells and preventing chemotherapy and radiotherapy resistance. Targeting CSCs has become a most effective approach to improve the cancer survival by eradicating the main root cause of cancer. The present review will addresses, in brief, the importance of cancer stem cells in targeting cancer as better and effective treatment along with a concluding outlook on the scope and challenges in the implication cancer stem cells in translational oncology.


PubMed | Truba Institute of Pharmacy
Type: Journal Article | Journal: Current protein & peptide science | Year: 2016

Hydroxyproline is a non-essential amino acid found in collagen and few other extracellular animal proteins. Its two isomeric forms trans-4-hydroxy-L-proline and trans-3-hydroxy-L-proline play a crucial role in collagen synthesis and thermodynamic stability of the triple-helical conformation of collagen and associated tissues. Various abnormalities in hydroxyproline metabolism have been shown to play key roles in the pathophysiology and pathogenesis of different diseases. The elevated level of hydroxyproline is observed in several disorders, e.g., graft versus host disease, keloids, and vitiligo while its decreased level is a marker of poor wound-healing. This review explores the potential of using hydroxyproline as a biochemical marker to understand the pathogenesis, molecular pathophysiology and treatment of these diseases. The review concludes with an outlook on the scope and challenges in the clinical implementation of hydroxyproline as a biomarker.

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