Entity

Time filter

Source Type


Nmorsi O.P.G.,Tropical Disease Reseach Unit | Isaac C.,Tropical Disease Reseach Unit | Ukwandu N.C.D.,Ambrose Alli University | Ohaneme B.A.,Tropical Disease Reseach Unit
Asian Pacific Journal of Tropical Medicine | Year: 2010

Objective: To examine array of some pro-and anti-inflammatory cytokines, namely, interleukin-4 (IL-4), interleukin-10 (IL-10), interferon-γ (IFN-γ), interleukin-5(IL-5), interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α) concentrations in some Nigerians with falciparum malaria. Methods: Sera were obtained from the blood samples of 96 Nigerian children with Plasmodium falciparum infection. The sera were subjected to cytokine evaluation using commercial standard enzyme linked immunosorbent assay kits (Abcam, UK). Results: Mean pro-inflammatory cytokines in serum of children with uncomplicated and complicated malaria were IL-5 482.2 pg/mL versus 526.7 pg/mL, IL-6 98.8 pg/mL versus 82.6 pg/mL, IL-12 24.1 pg/mL versus 15.9 pg/mL, TNF-α 107 pg/mL versus 511.7 pg/mL and IFN-γ 2.1 pg/mL versus 2.5 pg/mL. The anti-inflammatory cytokines status of IL-4 were 4.7 pg/mL versus 20.3 pg/mL, and IL-10 were 216 pg/mL versus 143.8 pg/mL in uncomplicated versus complicated/severe malaria cases. Participants with uncomplicated malaria had mean parasitaemia level of 3 158.9 parasites/μL while mean parasitaemia level for participants with complicated malaria was 12 550.5 parasite/μL and this difference was statistically significant (χ2 = 5 614.6, P < 0.05). The difference between mean haemoglobin level for uncomplicated malaria (9.6 g/dL) and severe malaria (3.9 g/dL) was statistically significant (χ2 = 2.3, P < 0.05). The relationship between serum level of IL-6, IL-12, IFN-γ, IL-10 and IL-4 and ages showed positive correlation at r=0.92, 0.99, 0.86, 0.95 and 0.85, respectively; while IL-5 and TNF-α had negative correlation at r=-0.99 and -0.99, respectively. Conclusion: IL-4, IL-5, IL-6, IL-10, IL-12, TNF-α and IFN-γ are involved in the immunopathology and immunoregulation of uncomplicated and complicated malaria infections. IL-6, IL-12, IFN-γ and IL-10 depressed in complicated/severe malaria may not provide any protective immunity and may be indicators of poor prognosis in Plasmodium falciparum infected Nigerian children. © 2010 Hainan Medical College. Source

Discover hidden collaborations