Tropical Crops Genetic Resources Institute

Danzhou, China

Tropical Crops Genetic Resources Institute

Danzhou, China
SEARCH FILTERS
Time filter
Source Type

Fu N.,Tropical Crops Genetic Resources Institute | Wu J.,Tropical Crops Genetic Resources Institute | Lv L.,Lanzhou Veterinary Research Institute | He J.,Lanzhou Veterinary Research Institute | Jiang S.,Tropical Crops Genetic Resources Institute
Brazilian Journal of Microbiology | Year: 2015

The foot and mouth disease virus (FMDV) is sensitive to acids and can be inactivated by exposure to low pH conditions. Spraying animals at risk of infection with suspensions of acid-forming microorganisms has been identified as a potential strategy for preventing FMD. Kombucha is one of the most strongly acid-forming symbiotic probiotics and could thus be an effective agent with which to implement this strategy. Moreover, certain Chinese herbal extracts are known to have broad-spectrum antiviral effects. Chinese herbal kombucha can be prepared by fermenting Chinese herbal extracts with a kombucha culture. Previous studies demonstrated that Chinese herbal kombucha prepared in this way efficiently inhibits FMDV replication in vitro. To assess the inhibitory effects of Chinese herbal kombucha against FMDV in vitro, swine challenged by intramuscular injection with 1000 SID50 of swine FMDV serotype O strain O/China/99 after treatment with Chinese herbal kombucha were partially protected against infection, as demonstrated by a lack of clinical symptoms and qRT-PCR analysis. In a large scale field trial, spraying cattle in an FMD outbreak zone with kombucha protected against infection. Chinese herbal kombucha may be a useful probiotic agent for managing FMD outbreaks. © 2015, Sociedade Brasileira de Microbiologia.


Niu J.H.,China Agricultural University | Niu J.H.,Tropical Crops Genetic Resources Institute | Jian H.,China Agricultural University | Guo Q.X.,China Agricultural University | And 4 more authors.
Plant Pathology | Year: 2012

A loop-mediated isothermal amplification (LAMP) assay for detection of Meloidogyne enterolobii (Me-LAMP) was developed based on the sequences of the 5S ribosomal DNA (5S rDNA) and intergenic spacer 2 (IGS2) segment. The LAMP amplification was achieved at 65°C isothermal conditions within 1-1·5h. Its amplicons were confirmed using gel electrophoresis, SacI enzyme analysis, lateral flow dipstick (LFD) assay, and visual inspection through SYBR Green I and calcein staining. The results demonstrated that the Me-LAMP was able to specifically detect M. enterolobii populations from different geographical origins, with a detection limit of about 10fg M. enterolobii genomic DNA, which was 10-100 times more sensitive than conventional PCR. In addition, the applicability of LAMP to field detection was confirmed following its successful performance in detecting the pest on root and soil samples. The Me-LAMP assay possessed the characteristics of simplicity, sensitivity and specificity, and is a promising and practical molecular tool for M. enterolobii diagnosis in pest quarantine and field surveys. © 2011 The Authors. Plant Pathology © 2011 BSPP.


Niu J.,China Agricultural University | Niu J.,Tropical Crops Genetic Resources Institute | Jian H.,China Agricultural University | Xu J.,China Agricultural University | And 4 more authors.
European Journal of Plant Pathology | Year: 2012

RNA interference (RNAi) techniques provide a major breakthrough in functional analysis for plant parasitic nematodes (PPNs). It offers the possibility of identifying new essential targets and consequently developing new resistance transgenes. To validate the potential of Mi-Rpn7 as a target for controlling root knot nematode Meloidogyne incognita and to evaluate the feasibility of our modified platform for assessing silencing phenotypes, we knocked down the Rpn7 gene of M. incognita using RNAi in vitro and in vivo. After soaking with 408-bp Rpn7 dsRNA, pre-parasitic second-stage juvenile (J2) nematodes showed specific transcript knockdown, resulting in an interrupted locomotion in an attraction assay with Pluronic gel medium, and consequently in a reduction of nematode infection ranging from 55. 2% to 66. 5%. With in vivo expression of Rpn7 dsRNA in transformed composite plants, the amount of egg mass per gram root tissue was reduced by 34% (P < 0. 05) and the number of eggs per gram root tissue was reduced by 50. 8% (P < 0. 05). Our results demonstrated that the silencing of the Rpn7 gene in M. incognita J2s significantly reduced motility and infectivity. Although it does not confer complete resistance, Mi-Rpn7 RNAi in hairy roots produced significant negative impacts on reproduction and motility of M. incognita. In addition, the presented modified procedure provides technique reference for PPN genes functional analysis or target screening. © 2012 KNPV.


PubMed | Tropical Crops Genetic Resources Institute and China Agricultural University
Type: | Journal: Scientific reports | Year: 2016

Root-knot nematodes (RKNs) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with their hosts. Nematode secretions, some of which have immunosuppressing activity, play essential roles in successful parasitism; however, their mechanisms of action remain largely unknown. Here, we show that the RKN-specific gene MiMsp40, cloned from Meloidogyne incognita, is expressed exclusively in subventral oesophageal gland cells and is strongly upregulated during early parasitic stages. Arabidopsis plants overexpressing MiMsp40 were more susceptible to nematode infection than were wild type plants. Conversely, the host-derived MiMsp40 RNAi suppressed nematode parasitism and/or reproduction. Moreover, overexpression of MiMsp40 in plants suppressed the deposition of callose and the expression of marker genes for bacterial elicitor elf18-triggered immunity. Transient expression of MiMsp40 prevented Bax-triggered defence-related programmed cell death. Co-agroinfiltration assays indicated that MiMsp40 also suppressed macroscopic cell death triggered by MAPK cascades or by the ETI cognate elicitors R3a/Avr3a. Together, these results demonstrate that MiMsp40 is a novel Meloidogyne-specific effector that is injected into plant cells by early parasitic stages of the nematode and that plays a role in suppressing PTI and/or ETI signals to facilitate RKN parasitism.


PubMed | Tropical Crops Genetic Resources Institute and China Agricultural University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott). The cloned sequences showed 99.9% identity and 80-88% similarity to the SPS sequences of other plants. The SPS gene of hybrid Pennisetum had one nucleotide and four amino acid polymorphisms compared to the other four germplasms, and cluster analysis was performed to assess genetic diversity in this species. Additional characterization of the SPS gene product can potentially allow Pennisetum to be exploited as a biofuel source.


Huang M.-Z.,Tropical Crops Genetic Resources Institute | Yin J.-M.,Tropical Crops Genetic Resources Institute | Yang G.-S.,Tropical Crops Genetic Resources Institute | Tan Y.-H.,CAS Xishuangbanna Tropical Botanical Garden
Phytotaxa | Year: 2012

A new species, Panisea moi (Orchidaceae: Epidedroideae: Coelogyninae) from Mount Wuzhi and Mount Jianfengling of Hainan Island is described and illustrated. The most significant differences from the closest species, P. vinhii, are that P. moi has subentire labellum, two longitudinal labellum keels that extend from middle of the hypochile to 2/3 of the length of the epichile and thickened at the ends; the rachis is also straight. © 2012 Magnolia Press.


Hou G.,Tropical Crops Genetic Resources Institute | Wang D.,Tropical Crops Genetic Resources Institute | Guan S.,Tropical Crops Genetic Resources Institute | Zeng H.,Tropical Crops Genetic Resources Institute | And 4 more authors.
Molecular Biology Reports | Year: 2010

A new SNP located in IGF-2 gene of Wuzhishan pig was detected while a C → T silent mutation at position 17 of exon 8 was detected by Single-Strand Conformational Polymorphism (SSCP). The value of polymorphism information content (PIC) indicated that this locus had intermediate polymorphism information content (0.25 < PIC < 0.5). The results of the fitness of Hardy-Weinberg equilibrium was in disequilibrium (P < 0.01). SAS analysis together with the multiple comparisons between polymorphisms and growth traits showed that: the differences of carcass weight (P = 0.024), withers height (P = 0.037) and chest girth (P = 0.025) in 10-month-old generation group were very remarkable. © 2009 Springer Science+Business Media B.V.


Hou G.-Y.,Tropical Crops Genetic Resources Institute | Zhao J.-M.,Chinese Institute of Scientific and Technical Information | Zhou H.-L.,Tropical Crops Genetic Resources Institute | Rong G.,Tropical Crops Genetic Resources Institute
Acta Tropica | Year: 2016

In the present study, the seroprevalence, risk factors and genotyping of Toxoplasma gondii in masked palm civet were investigated in tropical China. A total of 500 serum were collected from five administrative farms in tropical China, and assayed for T. gondii antibodies by modified agglutination test (MAT). The brain samples of 20 aborted fetuses were examined by semi-nested-PCR, and positive aborted fetuses (50%) were necropsied to collect the brain tissue for molecular and bioassay examinations. Genomic DNA was extracted from the 29 brain tissues of infected mice and T. gondii B1 gene was amplified using multilocus PCR-RFLP. Overall, 27.6% (95% CI: 23.682–31.518) of the animals was positive for T. gondii antibodies. Ages of masked palm civet was considered as a main risk factor associated with T. gondii infection. 4 DNA samples (13.8%) were positive for the T. gondii B1 gene. Three samples belong to ToxoDB#9, and one belongs to genotype the type II variant (ToxoDB genotype#3). Our results indicated that ToxoDB Genotype#9 has a distribution in masked palm civet that could be potential reservoirs for T. gondii transmission, which may pose a threat to human health. © 2016


Jun-hai N.,Tropical Crops Genetic Resources Institute | Yue-rong G.,Tropical Crops Genetic Resources Institute | Jun-mei Y.,Tropical Crops Genetic Resources Institute | Qing-yun L.,Tropical Crops Genetic Resources Institute | And 3 more authors.
European Journal of Plant Pathology | Year: 2015

Anthurium bacterial blight caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad) is an important and destructive disease worldwide, and no effective technique has been developed for its control. Detection of infection (latent) in anthurium plants is critical to evaluate disease progress and strengthening management to avoid a serious epidemic in the fields. In this paper, we presented a novel molecular method to detect Xad in anthurium using loop-mediated isothermal amplification (LAMP) technique (Xad-LAMP). The Xad-LAMP reaction could be finished by incubating at 61–65 °C for 1 h, and the amplificons were confirmed through gel electrophoresis, HpaII enzyme analysis, and visually inspected using SYBR Green I/calcein stain and lateral flow dipstick (LFD) assay. The specificity of Xad-LAMP primers set was widely validated on Xad and nontarget strains. In sensitivity testing, Xad-LAMP allowed detection as low as 1–10 f. pure genome DNA or 104 CFU/ml cells, 10–100 times more sensitive than conventional PCR. In addition, combining with the optimized DNA extraction, Xad-LAMP detections were successfully performed on both latent and disease samples, derived from artificially and naturally infected plants respectively. In all, this study provided a promising and practical molecular tool for Xad detecting, will facilitate the forecasting and control of anthurium blight disease. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.


PubMed | Tropical Crops Genetic Resources Institute and Chinese Institute of Scientific and Technical Information
Type: | Journal: Acta tropica | Year: 2016

In the present study, the seroprevalence, risk factors and genotyping of Toxoplasma gondii in masked palm civet were investigated in tropical China. A total of 500 serum were collected from five administrative farms in tropical China, and assayed for T. gondii antibodies by modified agglutination test (MAT). The brain samples of 20 aborted fetuses were examined by semi-nested-PCR, and positive aborted fetuses (50%) were necropsied to collect the brain tissue for molecular and bioassay examinations. Genomic DNA was extracted from the 29 brain tissues of infected mice and T. gondii B1 gene was amplified using multilocus PCR-RFLP. Overall, 27.6% (95% CI: 23.682-31.518) of the animals was positive for T. gondii antibodies. Ages of masked palm civet was considered as a main risk factor associated with T. gondii infection. 4 DNA samples (13.8%) were positive for the T. gondii B1 gene. Three samples belong to ToxoDB#9, and one belongs to genotype the type II variant (ToxoDB genotype#3). Our results indicated that ToxoDB Genotype#9 has a distribution in masked palm civet that could be potential reservoirs for T. gondii transmission, which may pose a threat to human health.

Loading Tropical Crops Genetic Resources Institute collaborators
Loading Tropical Crops Genetic Resources Institute collaborators