Trisaia Research Center

Rotondella, Italy

Trisaia Research Center

Rotondella, Italy
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Xu X.,Key Laboratory of Genomics | Pan S.,Key Laboratory of Genomics | Cheng S.,Key Laboratory of Genomics | Zhang B.,Key Laboratory of Genomics | And 111 more authors.
Nature | Year: 2011

Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and is central to global food security. It is clonally propagated, highly heterozygous, autotetraploid, and suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone to sequence and assemble 86% of the 844-megabase genome. We predict 39,031 protein-coding genes and present evidence for at least two genome duplication events indicative of a palaeopolyploid origin. As the first genome sequence of an asterid, the potato genome reveals 2,642 genes specific to this large angiosperm clade. We also sequenced a heterozygous diploid clone and show that gene presence/absence variants and other potentially deleterious mutations occur frequently and are a likely cause of inbreeding depression. Gene family expansion, tissue-specific expression and recruitment of genes to new pathways contributed to the evolution of tuber development. The potato genome sequence provides a platform for genetic improvement of this vital crop. © 2011 Macmillan Publishers Limited. All rights reserved.


Zappa G.,ENEA | Zappa G.,Trisaia Research Center | Gatti R.,ENEA | Gatti R.,Trisaia Research Center | And 10 more authors.
XXI IMEKO World Congress "Measurement in Research and Industry" | Year: 2015

This paper reports a feasibility study for the development of new Reference Materials (RMs) for mycotoxin analysis in cereals. In particular, different batches were prepared starting from lots of un-contaminated durum wheat grains, durum wheat flour, soft wheat grains, and soft wheat flour. Then, an aliquot of each raw material was artificially inoculated with Fusarium graminearum, so as to obtain DON and Ac-DON contamination. By mixing in different ratio the mycotoxin contaminated materials and the un-contaminated materials, test batches of (durum and soft) wheat grains and flour at three different levels of DON and Ac-DON (zero level, low level, high level) have been developed. Afterwards the characterization of the materials was carried out and stability and homogeneity studies have been started.

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