Treatment and Research Center for Infectious Diseases

Beijing, China

Treatment and Research Center for Infectious Diseases

Beijing, China

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Chen W.W.,Treatment and Research Center for Infectious Diseases
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To investigate the characteristics of γδ T cell subsets in peripheral blood and Vδ1 and Vδ2 T cell subsets proliferation after induction in vitro, and to provide experimental basis for γδ T cells expansion methods in vitro. Percentages of γδ T cells, Vδ1 and Vδ2 T cells in CD3 T cells from 25 cases of HIV/AIDS patients (HIV group) and 31 cases of healthy adults as control (HC group) were investigated with flow cytometry (FCM); and 10 cases peripheral blood mononuclear cells (PBMCs) from each group were induced and cultured for 14 d by using anti-γδ TCR mAb and IL-2, then the cell amounts were counted, γδ T cells and Vδ1, Vδ2 T cells were detected and analyzed with flow cytometry (FCM) at 0 d, 7 d and 14 d. The percentages and absolute counts of γδ T cells and V δ2 T cells in HIV group were significantly lower than those in HC group, but those of Vδ1 subsets were significantly higher than those of HC group. After culture for 14 day, the total cell amount of HC group expanded almost 3 times, but those of HIV group expanded a little; The percentages of γδ T cells in HC group were above 80%, but those in HIV group were only about 35%; the percentages of Vδ2 T cells in HC group were about 65%, but those in HIV group were only about 17%. The proportion of γδ T cells in peripheral blood of HIV/AIDS patients decreased significantly, and Vδ1/Vδ2 ratio was inversed; the expansion effect was not so good to use anti-γδ TCR mAb and IL-2 to induce γδ T cells and Vδ2 subsets of HIV/AIDS, and the Vδ1/Vδ2 ratio inversion could not reverse. More efficient culture methods should be explored.


PubMed | Treatment and Research Center for Infectious Diseases
Type: Journal Article | Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To investigate the characteristics of T cell subsets in peripheral blood and V1 and V2 T cell subsets proliferation after induction in vitro, and to provide experimental basis for T cells expansion methods in vitro.Percentages of T cells, V1 and V2 T cells in CD3 T cells from 25 cases of HIV/AIDS patients (HIV group) and 31 cases of healthy adults as control (HC group) were investigated with flow cytometry (FCM); and 10 cases peripheral blood mononuclear cells (PBMCs) from each group were induced and cultured for 14 d by using anti- TCR mAb and IL-2, then the cell amounts were counted, T cells and V1, V2 T cells were detected and analyzed with flow cytometry (FCM) at 0 d, 7 d and 14 d.The percentages and absolute counts of T cells and V 2 T cells in HIV group were significantly lower than those in HC group, but those of V1 subsets were significantly higher than those of HC group. After culture for 14 day, the total cell amount of HC group expanded almost 3 times, but those of HIV group expanded a little; The percentages of T cells in HC group were above 80%, but those in HIV group were only about 35%; the percentages of V2 T cells in HC group were about 65%, but those in HIV group were only about 17%.The proportion of T cells in peripheral blood of HIV/AIDS patients decreased significantly, and V1/V2 ratio was inversed; the expansion effect was not so good to use anti- TCR mAb and IL-2 to induce T cells and V2 subsets of HIV/AIDS, and the V1/V2 ratio inversion could not reverse. More efficient culture methods should be explored.

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