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Gainesville, FL, United States

Chen J.,Central South University of forestry and Technology | Chen J.,Transpoints Inc | Zhan P.,Central South University of forestry and Technology | Koopman B.,University of Florida | And 2 more authors.
Clean Technologies and Environmental Policy | Year: 2012

The Gordonia strain JW8 was successfully isolated, characterized, and tested for bioaugmentation of pulp and paper wastewater. With significant degradation of alkaline lignin, JW8 has the potential to render pulp and paper wastewater more biodegradable, which is of much interest to wastewater treatment. The sequencing batch reactor (SBR) inoculated with JW8 significantly enhanced the organic pollutants removal of pulp and paper wastewater compared to the control, achieving the best removal rates of 96.4% and 87.8% for biochemical oxygen demand (BOD) and chemical oxygen demand (COD), respectively. A considerable decrease of BOD/COD ratio in wastewater was also achieved in the bioaugmented SBR. However, a drawback caused by JW8 inoculation was the resultant high sludge volume index and biological foaming and bulking, which can be controlled by food/mass adjustment. The denaturing gradient gel electrophoresis analysis suggested that bioaugmentation with JW8 had a slight effect on the microbial dynamics, but more research is needed to understand the relationship between the microbial dynamics and pollutant removal. © US Government 2012. Source


Chen J.,Central South University of forestry and Technology | Chen J.,Transpoints Inc | Wang Q.,Central South University of forestry and Technology | Zhang L.,Central South University of forestry and Technology | Kong Q.,Central South University of forestry and Technology
Journal of Bionanoscience | Year: 2013

A low cost aqueous-based procedure was developed to prepare high quality CdSe QDs/chitosan- DNA complexes. Self-assembling of quantum dots (QDs) were used to label chitosan-DNA nanoparticles via electrostatic interaction. The optimal synthesis scheme was established by Fluorescence spectroscopy and Zetasizer Nano ZS. The nano transgenic system of QDs labeling chitosan-DNA complex was shown to have good fluorescence properties and uniform particle size. Cell suspension cultures of Jatropha curcas were established and optimized in shake flasks. The complex can be used as nano-scale gene carriers into cells of Jatropha curcas. Inverted fluorescent microscope (IFM) confirmed that less than 0.5 mg/L of BA and 2-3 minutes of ultrasound time would enhance the transformation efficiency. Copyright © 2013 American Scientific Publishers. All rights reserved. Source


Wang Q.,Central South University of forestry and Technology | Chen J.,Central South University of forestry and Technology | Chen J.,Transpoints Inc | Zhan P.,Central South University of forestry and Technology | And 2 more authors.
Advanced Materials Research | Year: 2013

Cell suspension cultures of Jatropha curcas were established and optimized in shake flasks. The stem segments of Jatropha curcas were taken as the explants for studying the techniques of callus induction and cell suspension cultures. The result shows that the optimal medium for callus induction is MS+2,4-D0.6mg/L+ BA1.0mg/L + Sucrose 30g/L, in which the callus is humid, loose and colorful. The fine suspension cell system have been established by inoculating the callus in the medium of MS+NAA0.2mg/L+2,4-D1.0mg/L+BA0.5mg/L for 13 days of cultivation, and the rotation speed should be lower than 120rpm in the culture of oscillation. QD-labeled chitosan-DNA complexes as nano transgenic system, using CdSe as bio-labels and chitosan-DNA(CS-DNA) as nano-scale genic carriers, were prepared and shown to have uniform particle sizes and superior fluorescence properties. Confocal laser scanning microscopy(CLSM) confirmed the target DNA from QD-labeled chitosan-DNA complexes was integrated into the plant cell and suggest the possibility of stable transformation in Jatropha curcas. © (2013) Trans Tech Publications, Switzerland. Source


Chen J.,Central South University of forestry and Technology | Chen J.,Transpoints Inc | Zhan P.,Central South University of forestry and Technology | Koopman B.,University of Florida | And 2 more authors.
International Journal of Green Energy | Year: 2015

Simultaneous saccharification and cofermentation (SSCF) experiments were conducted to optimize the conversion of paper sludge to ethanol using co-culture of Saccharomyces cerevisiae and Escherichia coli KO11. The fermentation parameters were optimized at 5 wt.% substrate via orthogonal experiments: 25 FPU/g cellulase, 38°C, 6 wt.% inoculums, and 1:1 S. cerevisiae/E. coli KO11. The ethanol conversion efficiency of co-culture was higher than that of single culture of S. cerevisiae or E. coli KO11 in 72 h fermentation. The maximum ethanol yield achieved by co-culture was 5.69 g/L, which corresponds to a theoretical conversion rate of 63.06 wt.%. The consumption of total reducing sugars reached 90.8%, with higher glucose utilization relative to xylose. © 2015 Taylor & Francis Group, LLC. Source


Chen J.,Central South University of forestry and Technology | Chen J.,Transpoints Inc | Liu B.,Central South University of forestry and Technology | Liu B.,Chinese Academy of Forestry | And 4 more authors.
Journal of Biobased Materials and Bioenergy | Year: 2015

An Agrobacteriuim-mediated Jatropha curcas transformation procedure has been optimized around high frequency leaf disc regeneration using phosphomannose isomerase (pmi) gene as biosafe selectable marker. A mannose to sucrose ratio of (20 g/10 g)·L-1 was found to be optimal as selection pressure. Cephalothin as a bacteriostat was optimized at 500 mg·L-1 for inhibition of Agrobacteriuim overgrowth without destructive effect on callus induction. The effect of acetosyringone concentration, inoculation duration, and co-cultivation time was evaluated and found to be most desirable at 0.02 mM, 10 min and 2 days, respectively. Sonication treatment had a suppressing effect on resistant shoots induction. The transformation efficiency with mannose selection was twice of that with kanamycin selection. PCR amplification identified 6 positive plants from 12 independent mannose-resistant plants. This efficient and biosafe transformation system is of significance to facilitating the genetic improvement of J. curcas as a promising biodiesel plant and also to alleviating public concerns on the wide use of transgenic energy plants. Copyright © 2015 American Scientific Publishers All rights reserved. Source

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