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Menoret S.,Transgenic Rats Nantes Ibisa Center National Of Recherche Scientifique | Menoret S.,Nantes University Hospital Center | Tesson L.,Transgenic Rats Nantes Ibisa Center National Of Recherche Scientifique | Tesson L.,Nantes University Hospital Center | And 15 more authors.
Methods | Year: 2014

The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models. © 2014 Elsevier Inc.


PubMed | French Natural History Museum and Transgenic Rats Nantes Ibisa Center National Of Recherche Scientifique
Type: | Journal: Methods in molecular biology (Clifton, N.J.) | Year: 2015

The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.

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