Traffic Hospital of Shandong Province

Jinan, China

Traffic Hospital of Shandong Province

Jinan, China
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Guan Y.-Y.,Traffic Hospital of Shandong Province | Song C.,Traffic Hospital of Shandong Province | Lei P.-S.,Peking Union Medical College
Journal of Asian Natural Products Research | Year: 2014

In order to simplify the synthesis of OSW-1's disaccharide side chain and explore the structure-activity relationship of OSW-1, three 16α-O-maltose OSW-1 analogs carrying three maltose side chains bearing different protections were designed and synthesized. © 2013 Taylor & Francis.


Guan Y.-Y.,Traffic Hospital of Shandong Province | Li S.-Z.,Traffic Hospital of Shandong Province | Lei P.-S.,Peking Union Medical College
Journal of Asian Natural Products Research | Year: 2016

During the process of icogenin analog research, we obtained two cytotoxic steroids: compound 4 and compound 6 casually. Their in vitro antitumor activities were tested by the standard MTT assay. The results disclosed that compound 4 (IC50 = 3.65–6.90 μM) showed potential antitumor activities against HELA, KB cell lines and compound 6 (IC50 = 2.40–9.05 μM) showed potential antitumor activities against HELA, BGC-823, KB, A549, HCT-8 cell lines. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Huang J.,Tianmen First Peoples Hospital | Ma X.-J.,Traffic Hospital of Shandong Province | Li Q.,Central Hospital of Zibo | Nie G.-J.,Central Hospital of Zibo
International Journal of Clinical and Experimental Medicine | Year: 2016

Background: The purpose of this study was to investigate the serum levels of mannose-binding lectin (MBL) in the Chinese patients with acute ischemic stroke (AIS). Method: We conducted a case-control study at the emergency department of our hospital. Serum MBL levels and routine test were examined. Its value for biomarker diagnosis was appreciated through receiver operating curve (ROC). The National Institutes of Health Stroke Scale (NIHSS) score was assessed on admission blinded to MBL levels. Results: The results indicated that the serum MBL levels were significantly (P<0.0001) higher in AIS patients as compared to normal controls (1452; IQR, 1030-2232 ug/L VS. 903; IQR, 678-1193 ug/L, respectively). There was a modest correlation between levels of serum MBL and NIHSS score (r=0.704, P<0.0001). The median MBL levels in patients with artery arteriosclerosis were significantly higher than other stroke subtype (1689[IQR, 1260-2785] vs. 1109[IQR, 915-1614] ug/L, respectively; P<0.0001). Based on the ROC curve, the optimal serum concentration of MBL as a surrogate marker to support the diagnosis of ischemic cerebral injury was found to be 1080 ug/L, which yielded a sensitivity of 79.4% and a specificity of 71.3%, the area under the curve was 0.764 (95% CI, 0.707-0.826). Conclusion: The discovery study presented here show serum MBL at admission are related with stroke severity and subtype. MBL levels also could be considered as an independent stroke diagnosis marker in Chinese population. © 2016, E-Century Publishing Corporation. All rights reserved.


Yang H.,Shandong University | Yang H.,Xintai People Hospital Affiliated to Taian Medical University | Lu Y.,Traffic Hospital of Shandong Province | Ma S.,Shandong University | And 4 more authors.
Journal of Stroke and Cerebrovascular Diseases | Year: 2015

Ischemic stroke is one of the leading causes of morbidity and mortality worldwide and characterized by defective angiogenesis. The functional sequences (RGDs, GRGDSPASSPISC) derived from fibronectin have been confirmed to augment angiogenesis in vivo and in vitro. However, delivery of peptides into the brain parenchyma has been hampered by the presence of the blood-brain barrier (BBB). We fused RGDs with penetratin (Antp) derived from Drosophila antennapedia homeodomain protein to improve the penetration of peptides through BBB into ischemic hemisphere. We found Antp-RGDs successfully not only penetrate the SH-SY5Y cells but also penetrated through BBB into ischemic hemisphere by intraperitoneal injection. In addition, application of Antp-RGDs to the focal cerebral ischemic reperfusion injury in rats resulted in the reduction of cerebral ischemic volume and the improvement of neurologic score according to the 21-point score. We further demonstrated that activation of phosphorylation-extracellular-signal related kinase 1/2 (p-ERK 1/2) and upregulation of gene VEGF resulted from post-treatment with Antp-RGDs 2 hours after reperfusion onset might at least partly contribute to the benefic changes after focal cerebral ischemic reperfusion injury in rats. Our data suggested that Antp-RGDs may serve as an attractive therapeutic intervention for treating ischemic stroke. © 2015 National Stroke Association.


Zhang Y.,Nanjing Medical University | Sun W.,Traffic Hospital of Shandong Province | Zhang F.,Nanjing Medical University | Huang J.,Nanjing Medical University | Fan Z.,Nanjing Medical University
Experimental and Therapeutic Medicine | Year: 2013

Pancreaticobiliary maljunction (PBM) is an unusual anomalous condition in which the pancreatic duct and bile duct merge outside the duodenal wall and form a long common channel. Pancreas divisum (PD) is a congenital anomaly in which the dorsal and ventral pancreatic ducts fail to fuse. Endoscopic retrograde cholangiopancreatography (ERCP) is the gold standard for diagnosing PD and magnetic resonance cholangiopancreatography (MRCP) is the non-invasive choice. In this study, four cases of patients with unusual PBM in addition to PD are described. The patients presented with abdominal pain, which was caused by distal biliary stricture diagnosed by MRCP. The patients received ERCP and had a good prognosis.


PubMed | Traffic Hospital of Shandong Province and Nanjing Medical University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2013

Pancreaticobiliary maljunction (PBM) is an unusual anomalous condition in which the pancreatic duct and bile duct merge outside the duodenal wall and form a long common channel. Pancreas divisum (PD) is a congenital anomaly in which the dorsal and ventral pancreatic ducts fail to fuse. Endoscopic retrograde cholangiopancreatography (ERCP) is the gold standard for diagnosing PD and magnetic resonance cholangiopancreatography (MRCP) is the non-invasive choice. In this study, four cases of patients with unusual PBM in addition to PD are described. The patients presented with abdominal pain, which was caused by distal biliary stricture diagnosed by MRCP. The patients received ERCP and had a good prognosis.


Zhang X.,Shandong University | Zhang Y.,Traffic Hospital of Shandong Province | Liu X.,Traffic Hospital of Shandong Province | Fang A.,Traffic Hospital of Shandong Province | And 5 more authors.
Oncotarget | Year: 2016

High recurrence rates of non-muscle invasive bladder cancer (NMIBC) in patients require lifelong testing and monitoring. The aim of this study is to develop a simplified RT-qPCR method (RT-qPCR-D) which directly quantifies cell-free miR-155 in urine without RNA extraction, and assess it as a potential tool in NMIBC detection. A pilot study including 60 urine samples was used to investigate the feasibility of RTqPCR- D in detecting cell-free miR-155. Then, miR-155 levels were quantified in a large independent cohort of urine from 162 NIMBC patients, 76 cystitis patients, and 86 healthy donors using the RT-qPCR-D method. Changes of cell-free miR-155 before and after operation were also analyzed in 32 NIMBC patients. In pilot study, we found a significant linear association between RT-qPCR and RT-qPCR-D in urinary miR-155 detection. Both methods showed cell-free miR-155 were significantly increased in NMIBC patients, and could reflect their expression in tissues. Then, the increased expression of cell-free miR-155 was successfully validated in 162 NIMBC patients when compared with cystitis patients and healthy donors. Moreover, it distinguished NMIBC patients from others with 80.2% sensitivity and 84.6% specificity, which was superior to urine cytology. Cell-free miR-155 correlated with NMIBC stage and grade, and was an independent factor for predicting recurrence and progression to muscle invasion. In addition, cell-free miR-155 was significantly decreased after NMIBC patients underwent transurethral bladder resection. In conclusion, detection of cell-free miR-155 in urine using RT-qPCR-D is a simple and noninvasive approach which may be used for NMIBC diagnosis and prognosis prediction.


Zhang X.,Shandong University | Yang X.,Shandong University | Zhang Y.,Traffic Hospital of Shandong Province | Liu X.,Traffic Hospital of Shandong Province | And 5 more authors.
Clinical Cancer Research | Year: 2015

Purpose: Cell-free Bmi-1 mRNA is stably detectable in the serum/plasma and is associated with the development and progression of some tumors. Previous methods detecting extracellular Bmi-1 mRNA with RNA extraction are inefficient. This study developed a novel reverse transcription quantitative PCR (RT-qPCR) approach directly applied in serum (RT-qPCR-D) to quantify Bmi-1 mRNA, and assessed its diagnostic and prognostic potential in colorectal cancer. Experimental Design: The feasibility of the RT-qPCR-D method was first analyzed in 50 serum samples. Then, using the RTqPCR- D method, Bmi-1 mRNA expression was validated in serum from an independent cohort of patients with 87 normal colonoscopy, 76 hyperplastic polyp, 82 inflammatory bowel disease, 68 adenoma, and 158 colorectal cancer. Receiver operating characteristic (ROC) curves and Cox analyses were used to evaluate its diagnosis and prognosis value, respectively. Results: In a pilot study, levels of Bmi-1 mRNA were increased in colorectal cancer serum samples detected by RT-qPCR-D and significantly associated with results obtained by RT-qPCR. In a validation cohort, serum Bmi-1 mRNA levels were significantly elevated in the colorectal cancer group and the adenoma group when compared with other groups. The area under ROC curve distinguishing colorectal cancer from benign colorectal diseases was 0.888, with 72.2% sensitivity and 94.9% specificity, which was superior to carcinoembryogenic antigen. Bmi-1 mRNA levels were significantly associated with survival. Cox analysis indicated Bmi-1 mRNA was an independent prognostic factor for overall survival. Conclusions: Detection of cell-free Bmi-1 mRNA in serum by RT-qPCR-D is a simple and noninvasive approach and may be used for colorectal cancer diagnosis and prognosis. © 2014 American Association for Cancer Research.


PubMed | Traffic Hospital of Shandong Province and Shandong University
Type: Journal Article | Journal: International journal of cancer | Year: 2016

EZH2 is overexpressed in bladder cancer (BC) and plays important roles in tumor development and progression. Recent studies show cell free (cf) RNAs released from cancer cells can reflect tissues changes and are stable and detectable in urine. Although conventional quantitative real-time PCR (qPCR) is highly sensitive, low abundances of urinary cf-RNAs usually result in false-negatives. Thus, this study develops a nested qPCR (nqPCR) approach to quantify cf-EZH2 mRNA in urine and further assess its clinical significance for BC. Forty urine samples were first selected to evaluate feasibility of nqPCR. Then, levels of urinary cf-EZH2 mRNA were detected using developed method in an independent cohort of subjects with 91 healthy, 81 cystitis, 169 nonmuscle invasive BC (NMIBC) and 103 muscle-invasive BC (MIBC). In cf-EZH2 mRNA detection, nqPCR method was significantly associated with qPCR, but it could detect more urine samples and increase detection limit three orders of magnitude. Based on nqPCR method, cf-EZH2 mRNA levels have been found to be increased in urine of NMIBC and MIBC patients (p<0.001). Compared with cytology, cf-EZH2 mRNA showed higher diagnostic ability for MIBC (p<0.001) while not for NMIBC (p>0.05). Moreover, it also could distinguish MIBC from NMIBC, with AUC of 0.787. For MIBC patients, high expression of cf-EZH2 mRNA associated with advanced stage and was an independent predictor of reduced disease free survival or overall survival. In conclusion, detection of cf-EZH2 mRNA in urine by nqPCR is a sensitive and noninvasive approach and may be used for diagnosis and prognosis prediction of MIBC.


PubMed | Traffic Hospital of Shandong Province and Shandong University
Type: Journal Article | Journal: PloS one | Year: 2015

Resistance to cisplatin-based chemotherapy is a major cause of treatment failure in advanced bladder cancer (BC) patients. There is increasing evidence that microRNAs are involved in the development and progression of BC. However, little is known about the function of microRNAs in predicting the effect of adjuvant chemotherapy on BC survival and regulating response to cisplatin. To address this issue, we employed RT-qPCR to evaluate the clinical significance of miR-203 expression in 108 tissues of BC patients receiving cisplatin-based adjuvant chemotherapy, and performed in vitro studies to explore chemotherapeutic sensitivity to cisplatin in miR-203 overexpressing BC cells. We found miR-203 levels were significantly lower in BC progression group than non-progression group (P<0.001). ROC curve analysis illustrated miR-203 could significantly distinguish progressed patients from those without progression (P<0.001), yielding an area under the ROC curve of 0.839 (95% CI, 0.756-0.903). Moreover, low miR-203 expression correlated with shortened progression free survival (PFS) and overall survival (OS) of BC patients, and was an independent prognostic factor. Overexpression of miR-203 in 5637 and T24 BC cells could decrease cell viability, enhance cisplatin cytotoxicity, and promote apoptosis. Western blotting and luciferase reporter assay showed Bcl-w and Survivin were direct downstream targets of miR-203. There was also a significant inverse association between miR-203 and Bcl-w or Survivin expression in BC tissues (r = -0.781, -0.740, both P<0.001). In conclusion, decreased miR-203 predicts progression and poor prognosis for BC patients treated with cisplatin-based chemotherapy while miR-203 overexpression can enhance cisplatin sensitization by promoting apoptosis via directly targeting Bcl-w and Survivin.

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