Time filter

Source Type

Yue Y.,Nantong University | Zhang D.,Nantong University | Zhang D.,Medial Research | Jiang S.,Nantong University | And 8 more authors.
Neurochemical Research | Year: 2014

LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NFκB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism. © The Author(s) 2014.


Xia X.-P.,Traditional Chinese Medical Hospital of Nantong City | Chen H.-L.,Nantong University | Cheng H.-B.,Traditional Chinese Medical Hospital of Nantong City
Spine | Year: 2013

STUDY DESIGN.: Systematic review of published prevalence of adjacent segment degeneration (ASD) after spine surgery. OBJECTIVE.: To evaluate the prevalence of ASD in patients after cervical and lumbar spine surgery. SUMMARY OF BACKGROUND DATA.: ASD is a common complication after spine surgery in long-term follow-up. A large body of literature has been reported on the topic, but no meta-analysis of the epidemiological data on ASD has been published METHODS.: We searched the MEDLINE until March 2012 published in English language that reported the prevalence of ASD after spine surgery. We determined the ASD rates by calculating proportions and 95% confidence interval (CI) for each study and then pooled the data to derive a pooled proportion and 95% CI. RESULTS.: A total of 94 studies with 34,716 patients from 19 countries were included. The occurrence of radiograph ASD ranged from 4.8% to 92.2%, and the pooled prevalence was 29.3% (95% CI, 22.7%-35.8%) by the random-effects model. The occurrence of symptoms ASD ranged from 0.0% to 30.3%, and the pooled prevalence was 7.4% (95% CI, 6.4%-8.5%). In cervical position, the occurrence of radiograph ASD and symptoms ASD was 32.8% (95% CI, 17.8%-47.9%) and 6.3% (95% CI, 4.8%-7.8%); in lumbar position, the occurrence of radiograph ASD and symptoms ASD was 26.6% (95% CI, 21.3%-31.9%) and 8.5% (95% CI, 6.4%-10.7%). In the 0.5- to 2- or less, more than 2- to 5- or less, and more than 5- to 20- or less year diagnosis time, the radiograph ASD prevalence was 21.8% (16.0%-27.6%), 33.6% (21.8%-45.4%), and 37.4% (10.7%-64.1%), respectively; and the symptoms ASD prevalence was 6.5% (4.8%-8.1%), 12.1% (8.2%-16.0%), and 3.2% (2.5%-4.0%), respectively. CONCLUSION.: Spine surgery is associated with significant risk of ASD. These figures may be useful in the estimation of the burden of the ASD after spine surgery. © 2013, Lippincott Williams & Wilkins.


Wang L.,Nanjing University | Wang L.,Nantong University | Yuan D.,Nantong University | Zhang D.,Nantong University | And 5 more authors.
Cellular and Molecular Neurobiology | Year: 2015

Exploring effective drugs that are capable of promoting nerve regeneration has gained much attention. Ginsenoside Re (Re) is the main ingredient of ginseng berries and roots. Research in the area has shown that ginsenoside Re exhibits multiple pharmacological activities via different mechanisms both in vivo and in vitro. But the potential therapeutic effects of Re on sciatic nerve crush injury (SNC) have been little investigated. Herein, we investigated the protect effect of Re on peripheral nerve regeneration in a rat SNC model. Walking track analysis revealed that Re treatment significantly promoted functional recovery of crushed sciatic nerve in rats. The expression of PCNA in rat sciatic nerve was up-regulated by Re treatment, and peaked when the concentration of Re was 2.0 mg/kg. Using immunofluorescent staining, we found that Re greatly increased the expression of GAP-43 and S100 in injured rat sciatic nerve. Furthermore, we evaluated the effects of Re on proliferation, differentiation, and migration of Schwann cells in SNC rat models. Our studies reveal that Re promotes nerve regeneration is depend on ERK1/2 and JNK1/2 signaling pathway. Elevated Oct-6 expression and featured morphological changes indicated that Re facilitated the differentiation of Schwann cells following SNC. Also, transwell and wound-healing assay demonstrated that the migration capabilities of Schwann cell were significantly enhanced after Re treatment. © 2015, Springer Science+Business Media New York.


Liu Z.,Nantong University | Zhang D.,Nantong University | Sun C.,Nantong University | Tao R.,Nantong University | And 5 more authors.
Inflammation | Year: 2015

Karyopherin-α2 (KPNA2) functions as an adaptor that transports several proteins to the nucleus. We investigated the function and possible mechanisms of KPNA2 involved in rheumatoid arthritis (RA). Western blotting and immunohistochemistry showed the protein expression of KPNA2 increased in synovial tissue of RA patients compared with the healthy controls. Double immunofluorescent staining indicated that KPNA2 co-localized with T cells, macrophage-like synoviocytes, fibroblast-like synoviocytes, and neutrophils in synovial tissue of RA patients. Moreover, the expression of KPNA2 in SW982 cells was increased in a time-dependent manner in response to TNFα stimulation. Both Western blotting and immunofluorescent staining assay revealed the co-localization of KPNA2 and P65 and their translocation from cytoplasma in TNFα-treated SW982 cells. Furthermore, knocking down the expression of KPNA2 by siRNA inhibited TNFα-induced expression of IL-6, MMP-1, and MMP-13 and, more importantly, decreased the P65 phosphorylation in SW982 cells. We therefore suggested that KPNA2 may play a key role in the inflammation process of RA via NF-κB P65 signal transduction pathway. © 2015, Springer Science+Business Media New York.


Zhang D.,Nantong University | Yue Y.,Nantong University | Jiang S.,Nantong University | Li A.,Nantong University | And 8 more authors.
Brain Research | Year: 2014

The glycinamide ribonucleotide transformylase (GART) gene, a trifunctional polypeptide, has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, and phosphoribosylaminoimidazole synthetase activity, and is required for de novo purine biosynthesis. GART is highly conserved in vertebrates. Alternative splicing of GART results in two transcript variants encoding different isoforms. However, the expression and function of GART in the central nervous system lesion are still unclear. In this study, we used a traumatic spinal cord injury (SCI) model in adult Sprague-Dawley rats and investigated the dynamic changes of GART protein expression in the spinal cord. Western blot analysis revealed that GART was present in sham-operated spinal cord. It gradually increased, reached a peak at day 3 after SCI, and then declined during the following days. Double immunofluorescence staining revealed a widespread of GART, and the majority of GARTs are detected in astrocytes. After injury, GART expression was increased predominantly in astrocytes, positively correlated with the highly expressed proliferating cell nuclear antigen (PCNA). Knockdown of GART expression in cultured primary astrocytes by siRNA revealed that expression of GART in astrocytes plays a role in the LPS-induced release of pro-inflammatory factors, such as TNF-α and IL-6. These results showed that GART may participate in the pathophysiology of SCI, and more research is needed to have a good understanding of its function and mechanism. © 2014 Elsevier B.V.


Xia X.,Traditional Chinese Medical Hospital of Nantong City | Zhou B.,Traditional Chinese Medical Hospital of Nantong City | Chen X.,Traditional Chinese Medical Hospital of Nantong City | Shen H.,Traditional Chinese Medical Hospital of Nantong City | And 3 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2016

Objective: Osteosarcoma is the most common primary malignant bone tumor. Conventional osteosarcoma is a primary intramedullary high grade malignant tumor, which occurred largely in children and young adult. Although combined treatment including adjuvant and neoadjuvant chemocherapy plus surgery has greatly improved survival of patients, the prognosis is poor with an initial mortality of 80%. There are abundant studies exploring the pathogenesis of osteosarcoma, however, the molecular mechanisms involved in the carcinogenesis, progression and prognosis are still unknown. Numbl, as a conserved homolog of Drosophila Numb, has been implicated in early development of the nervous system. Recently, its role in migration and invasion of tumors has been concerned. Methods: In this study, we investigated the expression of Numbl protein in a set of formalin fixed and paraffin embedded osteosarcoma tissue samples by using a specific anti-Numbl antibody. The relationship between the expression of Numbl and clinicopathologic parameters was analyzed. Results: We found that the expression level of Numbl protein reduced in osteosarcoma cells and down-regulation of Numbl was associated with the metastasis and a poor survival. Conclusions: Our data indicate that Numbl may be a molecular marker for prognosis and a potential therapeutic target in osteosarcoma.


PubMed | Traditional Chinese Medical Hospital of Nantong City and Nantong University
Type: Journal Article | Journal: Inflammation | Year: 2016

Pyrroloquinoline quinone (PQQ) is a naturally occurring redox cofactor that acts as an essential nutrient and antioxidant and has been reported to exert potent immunosuppressive effects. However, the therapeutically potential of PQQ on rheumatoid arthritis (RA) has not been explored. In the present study, the anti-inflammatory effects of PQQ were investigated in interleukin (IL)-1-treated SW982 cells, a RA-like fibroblast-like synoviocytes (FLSs) injury model. Our observations showed that pretreatment with PQQ significantly inhibited the expression of matrix metalloproteinase (MMP)-1 and MMP-3 and suppressed the production of proinflammatory mediators such as TNF- and IL-6 in IL-1-treated SW982 cells. The nuclear translocation of nuclear factor kappa B (NF-B) and the phosphorylation level of p65, p38, and JNK MAP kinase pathways were also inhibited by PQQ in IL-1-stimulated SW982 cells. To further confirm the therapeutic effects of PQQ on RA in vivo, a collagen-induced arthritis (CIA) model was used. Mice treated with PQQ demonstrated marked attenuation of arthritic symptoms based on histopathology and clinical arthritis scores. These results collectively suggested that PQQ might be a promising therapeutic agent for alleviating the progress of RA.


PubMed | Traditional Chinese Medical Hospital of Nantong City, Nantong University and Shandong University
Type: Journal Article | Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] | Year: 2015

To investigate the expression of Sam68 in articular cartilage of knee osteoarthritis (OA) and the relationship between Sam68 and NF-B activation and apoptosis signaling in OA articular chondrocytes.Sam68 expression in normal and osteoarthritic cartilage was assessed by immunohistochemistry and RT-PCR on both meniscal/ligamentous injury (MLI)-induced OA rat model and the clinical human OA cartilage tissues. Sam68 expression in chondrocytes under tumor necrosis factor-alpha (TNF-) stimuli was also assessed by immunoblot. Inhibiting Sam68 in chondrocytes under TNF- stimuli was conducted using small interfering RNA (siRNA) and its influence on the expression of apoptotic marker and catabolic genes was examined by immunoblot. The mechanism of how Sam68 stimulates NF-B activity was determined by co-immunoprecipitation and immunoblot analysis of nuclear and cytoplasmic fractions of TNF--treated chondrocytes for p65 and Sam68.Sam68 expression was increased in OA cartilage tissues and chondrocytes under TNF- stimuli. Inhibition of Sam68 by siRNA significantly decreased the expression of apoptotic markers (cleaved caspase-3 and cleaved PARP) in chondrocytes following TNF--stimulation. Sam68 knockdown suppressed I-B degradation and p65 nuclear transportation in TNF--treated chondrocytes, indicating a suppressed NF-B activation. Upon TNF- exposure, the nuclear transportation of Sam68 and its interaction with p65 was detected in chondrocytes. Furthermore, Sam68 knockdown also alleviated the TNF--induced catabolic marker (MMP13, ADAMTS5, iNOS and IL-6) expression.The highly expressed Sam68 promotes NF-B signaling activation, catabolic gene expression and cellular apoptosis in TNF--treated chondrocytes, which may provide better insights into the pathophysiology of OA and a potential target for its treatment.


PubMed | Traditional Chinese Medical Hospital of Nantong City
Type: Journal Article | Journal: Spine | Year: 2013

Systematic review of published prevalence of adjacent segment degeneration (ASD) after spine surgery.To evaluate the prevalence of ASD in patients after cervical and lumbar spine surgery.ASD is a common complication after spine surgery in long-term follow-up. A large body of literature has been reported on the topic, but no meta-analysis of the epidemiological data on ASD has been publishedWe searched the MEDLINE until March 2012 published in English language that reported the prevalence of ASD after spine surgery. We determined the ASD rates by calculating proportions and 95% confidence interval (CI) for each study and then pooled the data to derive a pooled proportion and 95% CI.A total of 94 studies with 34,716 patients from 19 countries were included. The occurrence of radiograph ASD ranged from 4.8% to 92.2%, and the pooled prevalence was 29.3% (95% CI, 22.7%-35.8%) by the random-effects model. The occurrence of symptoms ASD ranged from 0.0% to 30.3%, and the pooled prevalence was 7.4% (95% CI, 6.4%-8.5%). In cervical position, the occurrence of radiograph ASD and symptoms ASD was 32.8% (95% CI, 17.8%-47.9%) and 6.3% (95% CI, 4.8%-7.8%); in lumbar position, the occurrence of radiograph ASD and symptoms ASD was 26.6% (95% CI, 21.3%-31.9%) and 8.5% (95% CI, 6.4%-10.7%). In the 0.5- to 2- or less, more than 2- to 5- or less, and more than 5- to 20- or less year diagnosis time, the radiograph ASD prevalence was 21.8% (16.0%-27.6%), 33.6% (21.8%-45.4%), and 37.4% (10.7%-64.1%), respectively; and the symptoms ASD prevalence was 6.5% (4.8%-8.1%), 12.1% (8.2%-16.0%), and 3.2% (2.5%-4.0%), respectively.Spine surgery is associated with significant risk of ASD. These figures may be useful in the estimation of the burden of the ASD after spine surgery.


PubMed | Traditional Chinese Medical Hospital of Nantong City
Type: Journal Article | Journal: The Journal of sports medicine and physical fitness | Year: 2015

The aim of the paper is to systematically review the diagnostic accuracy of ultrasonography for meniscal injuries in knee joint.A search of PubMed and Web of Science was performed up to January 2015. Only studies for diagnosis meniscal injuries by ultrasonography were included. The results were meta-analyzed by pooling estimates of sensitivity, specificity, diagnostic odds ratio (DOR), and constructing the summary receiver operating characteristic (SROC) curves.Twenty-one studies met the selection criteria for inclusion in the analysis. The pooled estimates for sensitivity, specificity, and area under curve (AUC) of ultrasonography diagnosis were 0.775 (95% CI: 0.747-0.801), 0.838 (95% CI: 0.818-0.857), and 0.9107 (95% CI: 0.8625-0.9589), respectively. For lateral meniscus diagnosis and medial meniscus diagnosis, the AUC was 0.9812 (95% CI: 0.9592-1.000) and 0.8997 (95% CI: 0.7313-1.000), respectively. However, in diagnosis by magnetic resonance imaging (MRI), the AUC was 0.9421 (95% CI: 0.8760-1.000). The ultrasonography inter-observer agreement resulted in a moderate reliability, with the value ranged from 0.381 to 0.482.We found the diagnostic accuracy of ultrasonography for meniscal injuries was good. No evidence showed MRI had better accuracy than ultrasonography diagnosis. We recommend ultrasonography should be routinely used for evaluation of meniscal injuries in knee joint.

Loading Traditional Chinese Medical Hospital of Nantong City collaborators
Loading Traditional Chinese Medical Hospital of Nantong City collaborators