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Nishi-Tokyo-shi, Japan

Sillapakong P.,Iwate University | Yang P.,Iwate University | Yang P.,Nihon Preventive Medical Laboratory Co. | Sato Y.,Iwate University | And 9 more authors.
Journal of Insect Biotechnology and Sericology | Year: 2012

We separated an aqueous extract of Morus alba stem bark by gel filtration chromatography, ammonium sulfate fractionation, and chromatography on Superdex 200 10/300 GL and Mono Q 5/50 GL columns. The resultant water-soluble fraction significantly increased total lymphocyte proliferation up to 3-fold (at 10 μg/ml) and stimulated the proliferation of isolated B cells (at 5, 10, and 20 μg/ml), similar to the positive control (lipopolysaccharide). This fraction, with an apparent high molecular weight of ≥ 200 k, consisted of 55.3% proteins and 9.6% sugars. The bioactive fraction treated with trypsin and pre-incubated either alone or with 2-mercaptoethanol and glycopeptitase F significantly reduced lymphocyte proliferation. These results suggest that a water-soluble glycoprotein-like complex of M. alba stem bark has therapeutic potential to boost immunity.

Sillapakong P.,Iwate University | Yamamoto H.,Iwate University | Mangetsu M.,Toyotama Healthy Food Co. | Noda S.,Toyotama Healthy Food Co. | And 7 more authors.
Journal of Insect Biotechnology and Sericology | Year: 2012

All parts of Morus alba offer great therapeutic potential, but are they beneficial to the whole organism, particularly with respect to aging? We evaluated the effect of an ethanol extract of mulberry leaves on the model organism Caenorhabditis elegans. The extract significantly extended mean lifespan by 17% from 17.6 to 20.6 days. This compares well to the effect of ethosuximide, a drug used to treat seizures in humans, which increased adult lifespan by 35%. This novel activity suggests that mulberry leaves have the potential to retard the aging process.

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