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Shimomura N.,Tottori University | Matsuda M.,Tottori University | Ariyoshi K.,Tottori Prefectural Agriculture and Forest Research Institute | Aimi T.,Tottori University

Rhizopogon roseolus (Corda) Th. M. Fr., known as shoro in Japanese, is an edible ectomycorrhizal mushroom. To successfully cultivate ectomycorrhizal mushrooms, inoculation and propagation methods must be developed, and isolates with superior traits must be selected. Pinus thunbergii Parl. seedlings were inoculated with several isolates of R. roseolus using the Petri dish technique and their mycorrhizal abilities were assessed. We selected a superior isolate that rapidly colon- ized and produced a lot of ectomycorrhizas in the roots of P. thunbergii. The selected strain was cultured in liquid medium, and the resultant mycelia were homogenized in saline solution to make a slurry. Adding surfactant to the mycelial slurry stimulated mycorrhizal formation in host roots. We investigated the effects of mycelial slurry containing surfactant on ecto- mycorrhizal formation of P. thunbergii and fruiting body formation in mother plant systems. Stimulatory effects of the slurry were observed not only on ectomycorrhizal formation of the seedlings but also on fruiting body formation. These results suggest that the mycelial slurries containing surfactant could serve as mycelial spawns for the cultivation of shoro under greenhouse conditions. Source

Kimura R.,Tottori University | Moriyama M.,Nagasaki University | Bandou S.,Tottori Prefectural Agriculture and Forest Research Institute
International Journal of Remote Sensing

Numerous studies have suggested that rice quality in Japan is affected by high temperatures during the ripening period, especially in summer. On the assumption that land-surface temperature (LST) can be substituted for air temperature, we examined rice quality using LST satellite data from the Moderate Resolution Imaging Spectroradiometer (MODIS) during the ripening stage (August) in Tottori prefecture. Rice quality in Tottori was very low compared with Japan as a whole and to neighbouring prefectures. LST was correlated with minimum and average air temperatures in August at six meteorological stations in Tottori. Rice quality decreased with increasing LST, and the threshold LST value when the quality of rice was less than 50% was 307 K (33.9 °C). The spatial distribution of LST in August indicated that LST values over 307 K were widespread, especially in coastal and lowland areas, and areas with the highest rice quality corresponded with intermountain regions that had LSTs less than 307 K. © 2015 Taylor & Francis. Source

Nishikawa T.,Kyoto University | Nishikawa T.,Tottori Prefectural Agriculture and Forest Research Institute | Kido K.,Kyoto University | Kido K.,Rengo Co. | And 4 more authors.
Plant Production Science

A field experiment assessing the effect of the annual application of anaerobically-digested cattle manure (ADM), produced at a biogas plant, on paddy rice was conducted. In plots with ADM (MF), the early growth of rice plants, from transplanting to the active tillering stage, was inhibited compared to the plots with chemical fertilizers (CF). This phenomenon was observed over many growing seasons and was especially obvious in nitrogen uptake and leaf area index (LAI). However, after panicle initiation, the growth of MF-treated plants was equal to or superior to CF-treated plants. The grain yield in all the MF plots was 96 - 105% of that in the CF plots. The inorganic nitrogen content of the soil in the MF plots was higher than that in the CF plots, which was contradictory to the growth inhibition observed in the initial growth of plants in the MF plots. In contrast, the oxidation/reduction potential and pH of the surface soil in MF plots were within the normal range, indicating that these soil factors were not associated with growth inhibition observed in MF plots. Our results implied that rice cultivars with a long growing period that are able to recover from the initial growth inhibition, such as medium or late maturing cultivars, are suitable for paddy rice production fertilized with ADM. Source

Yoshimura Y.,Tottori University | Ido A.,Tottori Prefectural Agriculture and Forest Research Institute | Iwase K.,Teikyo University | Matsumoto T.,Tottori University | Yamato M.,Tottori University
Microbes and Environments

We examined the colonization rate and communities of arbuscular mycorrhizal fungi (AMF) in the roots of Pyrus pyrifolia var. culta (Japanese pear) in orchards to investigate the effect of phosphorus (P) fertilization on AMF. Soil cores containing the roots of Japanese pear were collected from 13 orchards in Tottori Prefecture, Japan. Soil-available P in the examined orchards was 75.7 to 1,200 mg kg-1, showing the extreme accumulation of soil P in many orchards. The AMF colonization rate was negatively correlated with soil-available P (P <0.01). AMF communities were examined on the basis of the partial fungal DNA sequences of the nuclear small-subunit ribosomal RNA gene (SSU rDNA) amplified by AMF-specific primers AML1 and AML2. The obtained AMF sequences were divided into 14 phylotypes, and the number of phylotypes (species richness) was also negatively correlated with soil-available P (P <0.05). It was also suggested that some AM fungi may be adapted to high soil-available P conditions. Redundancy analysis showed the significant effects of soil pH, available P in soil, and P content in leaves of P. pyrifolia var. culta trees on AMF distribution. These results suggested that the accumulation of soil-available P affected AMF communities in the roots of Japanese pear in the orchard environment. Source

Naka H.,Tottori University | Mochizuki M.,National Institute of Fruit Tree Science | Nakada K.,Tottori Prefectural Agriculture and Forest Research Institute | Due Do N.,Tokyo University of Agriculture and Technology | And 3 more authors.
Bioscience, Biotechnology and Biochemistry

In field screening tests of synthetic pheromone candidates for Japanese sesiid species, a mixture of (3Z,13Z)-octadecadien-l-ol and (3Z,13Z)- octadecadienyl acetate successfully attracted male moths of Glossosphecia romanovi, a harmful pest of vine trees. The GC-EAD and GC-MS analyses of the pheromone gland extract revealed that the female moths produced the alcohol and acetate in a ratio of about 20:1, in addition to three other minor structure-related components. Source

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