Toronto General Hospital Toronto

Toronto, Canada

Toronto General Hospital Toronto

Toronto, Canada

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Hatta K.,Toronto General Hospital Toronto | Hatta K.,University of Toronto | Macleod R.J.,Queen's University | Gerber S.A.,University of Rochester | Croy B.A.,Queen's University
American Journal of Reproductive Immunology | Year: 2012

Problem: Understanding of uterine natural killer (uNK) cell functions during normal pregnancy remains incomplete. Method of study: Cloud tag analysis of literature was used to document themes addressed experimentally for uNK cells. Immunohistochemistry, including whole-mount staining of early implantation sites, separation of uNK cells into molecularly distinct subsets, and physiologic measurements in normal and mutant mice, are further advancing understanding of uNK cell biology. Results: Literature analyses revealed three key, current uNK cell research themes: angiogenesis, spiral arterial remodeling/pre-eclampsia/hypertension and infertility. UNK cells are being defined as cells potentially regulated by Wnt signaling that are heterogeneous in progenitor source and function and make unique contributions to implantation site development prior to spiral arterial remodeling. Conclusion: Future studies are poised to define uNK cell progenitor cells, identify the signaling pathways supporting established uNK cell functions and move current understanding of mouse uNK cells to clinical research questions. © 2012 John Wiley & Sons A/S.


Balasundaram K.,Ryerson University | Masse S.,Toronto General Hospital Toronto | Nair K.,Toronto General Hospital Toronto | Umapathy K.,Ryerson University
Medical and Biological Engineering and Computing | Year: 2013

Identification and classification of ventricular arrhythmias such as rhythmic ventricular tachycardia (VT) and disorganized ventricular fibrillation (VF) are vital tasks in guiding implantable devices to deliver appropriate therapy in preventing sudden cardiac deaths. Recent studies have shown VF can exhibit strong regional organizations, which makes the overlap zone between the fast paced rhythmic VT and VF even more ambiguous. Considering that implantable cardioverter-defibrillator (ICD) are primarily rate dependent detectors of arrhythmias and that there may be patients who suffer from arrhythmias that fall in the overlap zone, it is essential to identify the degree of affinity of the arrhythmia toward VT or organized/disorganized VF. The method proposed in this work better categorizes the overlap zone using Wavelet analysis of surface ECGs. Sixty-three surface ECG signal segments from the MIT-BIH database were used to classify between VT, organized VF (OVF), and disorganized VF (DVF). A two-level binary classifier was used to first extract VT with an overall accuracy of 93.7 % and then the separation between OVF and DVF with an accuracy of 80.0 %. The proposed approach could assist clinicians to provide optimal therapeutic solutions for patients in the overlap zone of VT and VF. © 2012 International Federation for Medical and Biological Engineering.


PubMed | Toronto General Hospital Toronto
Type: Journal Article | Journal: American journal of reproductive immunology (New York, N.Y. : 1989) | Year: 2012

Understanding of uterine natural killer (uNK) cell functions during normal pregnancy remains incomplete.Cloud tag analysis of literature was used to document themes addressed experimentally for uNK cells. Immunohistochemistry, including whole-mount staining of early implantation sites, separation of uNK cells into molecularly distinct subsets, and physiologic measurements in normal and mutant mice, are further advancing understanding of uNK cell biology.Literature analyses revealed three key, current uNK cell research themes: angiogenesis, spiral arterial remodeling/pre-eclampsia/hypertension and infertility. UNK cells are being defined as cells potentially regulated by Wnt signaling that are heterogeneous in progenitor source and function and make unique contributions to implantation site development prior to spiral arterial remodeling.Future studies are poised to define uNK cell progenitor cells, identify the signaling pathways supporting established uNK cell functions and move current understanding of mouse uNK cells to clinical research questions.

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