Tooth Bank

Brownsburg, IN, United States

Tooth Bank

Brownsburg, IN, United States

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Kim Y.-K.,Seoul National University | Um I.-W.,Tooth Bank | Murata M.,Health Sciences University of Hokkaido
Journal of Hard Tissue Biology | Year: 2014

The use of auto-tooth bone grafts fabricated from patients' own extracted teeth has become possible due to the development of tooth banking procedures. The Korea Tooth Bank (KTB), established in Seoul in 2009, is one such tooth-banking facility that can procure and store teeth, and then process them into bone graft substitutes. Another is the Hospital Tooth Bank (HTB) at Seoul National University Bundang Hospital (SNUBH), established in 2010 for performing storage and grafting of auto-tooth bone grafts based on experimental and clinical research. Extracted teeth are sent to the above-mentioned facility units and then delivered back to the patients for clinical use, and thus the safety of the auto-tooth bone graft materials as well as the clinical effectiveness must be guaranteed through proper quality assurance (QA) procedures. For the purpose of this investigation, we analyzed written documents for QA at KTB, and we performed histopathologic and microbiologic examinations against the banked tooth materials at the HTB. The results suggest that the tooth banking systems at both KTB and HTB sufficiently ensure patient safety. © 2014 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.


Kim Y.-K.,Seoul National University | Lee J.-H.,Seoul National University | Um I.-W.,Tooth Bank | Cho W.-J.,Tooth Bank
Journal of Oral and Maxillofacial Surgery | Year: 2016

Purpose This case report reviews the long-term clinical outcomes of using demineralized dentin matrix (autogenous tooth bone graft material [AutoBT]) in 5 cases that were first reported in 2010. Materials and Methods Cone-beam computerized tomography was used to measure the height and width of the graft to determine the change in bone area from immediately after surgery to final follow-up (average, 5 yr 5.8 months). Corticocancellous bone formation and marginal bone resorption also were evaluated histologically 3 to 6 months after grafting, which focused mainly on remodeling capacities. Results Decreases in buccal height and alveolar ridge width ranged from -0.4 to -3.3 mm and from -0.4 to -4.2 mm, respectively. The change in bone area ranged from -8.1 to -36.2%. Corticocancellous bone had formed and was maintained successfully except for 1 mm of buccal marginal bone resorption in 1 case followed for 6 years 7 months. Conclusion AutoBT, which was first reported for guided bone regeneration, showed that the corticocancellous bone that had formed had been maintained successfully with an implant after an average follow-up of 5 years. Although the number of samples was small, the results were consistent with those of other short-term follow-up studies on AutoBT. © 2016 American Association of Oral and Maxillofacial Surgeons.


Kim Y.-K.,Seoul National University | Lee J.-H.,Seoul National University | Um I.-W.,Tooth Bank | Cho W.-J.,Tooth Bank
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons | Year: 2016

PURPOSE: This case report reviews the long-term clinical outcomes of using demineralized dentin matrix (autogenous tooth bone graft material [AutoBT]) in 5 cases that were first reported in 2010.MATERIALS AND METHODS: Cone-beam computerized tomography was used to measure the height and width of the graft to determine the change in bone area from immediately after surgery to final follow-up (average, 5 yr 5.8 months). Corticocancellous bone formation and marginal bone resorption also were evaluated histologically 3 to 6 months after grafting, which focused mainly on remodeling capacities.RESULTS: Decreases in buccal height and alveolar ridge width ranged from -0.4 to -3.3 mm and from -0.4 to -4.2 mm, respectively. The change in bone area ranged from -8.1 to -36.2%. Corticocancellous bone had formed and was maintained successfully except for 1 mm of buccal marginal bone resorption in 1 case followed for 6 years 7 months.CONCLUSION: AutoBT, which was first reported for guided bone regeneration, showed that the corticocancellous bone that had formed had been maintained successfully with an implant after an average follow-up of 5 years. Although the number of samples was small, the results were consistent with those of other short-term follow-up studies on AutoBT. Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.


Kim Y.-K.,Seoul National University | Kim S.-G.,Chosun University | Um I.-W.,Tooth Bank | Kim K.-W.,Dankook University
Implant Dentistry | Year: 2013

OBJECTIVE:: A case study was conducted to examine the clinical results and histologic healing of bone grafts performed using an autogenous tooth block (AutoBT block), which was developed recently and proprietary. STUDY DESIGN:: Guided bone regeneration, extraction socket graft, sinus bone graft, and ridge augmentation were performed using autogenous tooth block graft material in 12 patients from March 2009 to June 2010. The clinical outcomes of each case were examined, and tissue specimens were collected from 1 case 2.5 months after the bone graft for histopathological analysis. RESULTS:: All of the cases had successful bone graft results. One patient developed wound dehiscence after surgery, although favorable secondary healing was achieved. One implant resulted in osseointegration failure. A histopathologic examination was performed after 2.5 months and showed excellent bone healing due to osteoconduction. The AutoBT block was incorporated into the upper soft tissue, aponeurosis, and lower recipient bone. CONCLUSION:: There were no notable complications associated with the bone transplant materials. The AutoBT block is clinically useful for a variety of bone grafts. Copyright © 2013 by Lippincott Williams & Wilkins.


Nam H.,Dental Research Institute | Kim J.,Dental Research Institute | Park J.,Dental Research Institute | Park J.-C.,Seoul National University | And 4 more authors.
Molecules and Cells | Year: 2011

Hertwig's epithelial root sheath/Epithelial rests of Malassez (HERS/ERM) cells are unique epithelial cells in the periodontal ligament. They remain in periodontal tissues throughout the adult life, and it is expected that their functional role is to maintain the homeostasis of the periodontium through reciprocal interactions with other periodontal cells. In this study, we investigated whether HERS/ERM cells have primitive stem cell characteristics: those of embryonic stem cells as well as of epithelial stem cells. Primary HERS/ERM cells had typical epithelial cell morphology and characteristics and they maintained for more than five passages. They expressed epithelial stem cell-related genes: ABCG2, ΔNp63, p75, EpCAM, and Bmi-1. Moreover, the expression of embryonic stem cell markers such as Oct-4, Nanog, and SSEA-4 were detected. Next, we investigated whether the expression of these stem cell markers was maintained during the sub-culture process. HERS/ERM cells showed different expression levels of these stemness genes at each passage, but their expression was maintained throughout the passages. Taken together, our data suggest that a primary culture of HERS/ERM cells contains a population of primitive stem cells that express epithelial stem cell markers and embryonic stem cell markers. Furthermore, these cell populations were maintained during the sub-culturing process in our culture conditions. Therefore, our findings suggest that there is a strong possibility of accomplishing cementum tissue engineering with HERS/ERM cells.


Kim Y.-K.,Seoul National University | Kim S.-G.,Chosun University | Yun P.-Y.,Seoul National University | Yeo I.-S.,Seoul National University | And 9 more authors.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology | Year: 2014

Objectives: This study evaluated the surface structures and physicochemical characteristics of a novel autogenous tooth bone graft material currently in clinical use. Study Design: The material's surface structure was compared with a variety of other bone graft materials via scanning electron microscope (SEM). The crystalline structure of the autogenous tooth bone graft material from the crown (AutoBT crown) and root (AutoBT root), xenograft (BioOss), alloplastic material (MBCP), allograft (ICB), and autogenous mandibular cortical bone were compared using x-ray diffraction (XRD) analysis. The solubility of each material was measured with the Ca/P dissolution test. Results: The results of the SEM analysis showed that the pattern associated with AutoBT was similar to that from autogenous cortical bones. In the XRD analysis, AutoBT root and allograft showed a low crystalline structure similar to that of autogenous cortical bones. In the CaP dissolution test, the amount of calcium and phosphorus dissolution in AutoBT was significant from the beginning, while displaying a pattern similar to that of autogenous cortical bones. Conclusions: In conclusion, autogenous tooth bone graft materials can be considered to have physicochemical characteristics similar to those of autogenous bones. © 2014 Elsevier Inc.


PubMed | Dankook University, Korea University, Tooth Bank and Seoul National University
Type: Journal Article | Journal: Journal of the Korean Association of Oral and Maxillofacial Surgeons | Year: 2016

The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2.Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (CT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals.Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The CT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks.Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.


The aim of this study was to prospectively evaluate the clinical efficacy and histological outcome of the autogenous tooth graft material (AutoBT) compared to that of anorganic bovine bone (Bio-OssA total of 33 graft sites in 24 patients were included in this study. AutoBT was used in 21 sites of 15 patients and Bio-OssInfection of graft material or graft bed was not observed and graft sites healed without any notable complications in both groups. The vertical dimensions of alveolar bone increased by 5.382.65mm in AutoBT group and 6.563.54mm in Bio-OssAutogenous demineralized dentin matrix from extracted tooth grafted to extraction sockets for the augmentation of vertical dimension was as effective as augmentation using anorganic bovine bone. Both groups showed favorable wound healing, similar amount of implant stability, and histologically confirmed new bone formation. Thus, the results of this study suggest that autogenous tooth graft material is a viable option for alveolar bone augmentation following dental extraction.


PubMed | Ajou University, Kure Kyosai Hospital, Health Sciences University of Hokkaido, Tooth Bank and Seoul National University
Type: Journal Article | Journal: Journal of the Korean Association of Oral and Maxillofacial Surgeons | Year: 2015

In cases of severe alveolar bone atrophy in the posterior maxillary area, which has only a thin sinus floor, the autogenous tooth bone graft block (ABTB) was used to wrap the implant to enhance its primary stability and osseointegration in the sinus. These cases with four years of clinical follow-up demonstrate the applicability of the ABTB in maxillary sinus membrane elevation to improve the outcomes of implant placement.


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