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Brownsburg, IN, United States

Kim Y.-K.,Seoul National University | Kim S.-G.,Chosun University | Um I.-W.,Tooth Bank | Kim K.-W.,Dankook University
Implant Dentistry

OBJECTIVE:: A case study was conducted to examine the clinical results and histologic healing of bone grafts performed using an autogenous tooth block (AutoBT block), which was developed recently and proprietary. STUDY DESIGN:: Guided bone regeneration, extraction socket graft, sinus bone graft, and ridge augmentation were performed using autogenous tooth block graft material in 12 patients from March 2009 to June 2010. The clinical outcomes of each case were examined, and tissue specimens were collected from 1 case 2.5 months after the bone graft for histopathological analysis. RESULTS:: All of the cases had successful bone graft results. One patient developed wound dehiscence after surgery, although favorable secondary healing was achieved. One implant resulted in osseointegration failure. A histopathologic examination was performed after 2.5 months and showed excellent bone healing due to osteoconduction. The AutoBT block was incorporated into the upper soft tissue, aponeurosis, and lower recipient bone. CONCLUSION:: There were no notable complications associated with the bone transplant materials. The AutoBT block is clinically useful for a variety of bone grafts. Copyright © 2013 by Lippincott Williams & Wilkins. Source

Kim Y.-K.,Seoul National University | Um I.-W.,Tooth Bank | Cho W.-J.,Tooth Bank | Murata M.,Health Sciences University of Hokkaido | And 2 more authors.
Journal of Hard Tissue Biology

The purpose of this study is to evaluate an ability of a moldable autogenous tooth bone graft material (M-AutoBT), which is made of demineralized dentin matrix (DDM) and hydroxypropylmethyl cellulose (HPMC) to repair sinus-related defects in the implant dentistry. Sinus-related alveolar defects were classified into three types depending on the remnant of socket wall as follows; Class I: Only alveolar bone repair is required even if sinus cavity is involved (remained socket wall > 5mm). Class II: Both alveolar bone and sinus repair are required (3mm < remained socket wall < 5mm). Class III: Only sinus repair is required without any alveolar bone (remained socket wall < 3mm). Sinus cavity should always be involved during the implant placement regardless of defect types. The histological examination of three case reports, which represented class I, II and III defect respectively, showed osteoconductive and osteoinductive functions of M-AutoBT that is similar to the effect of the conventional AutoBT. Therefore, HPMC might be the suitable base material for AutoBT. © 2015 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved. Source

Kim Y.-K.,Seoul National University | Um I.-W.,Tooth Bank | Murata M.,Health Sciences University of Hokkaido
Journal of Hard Tissue Biology

The use of auto-tooth bone grafts fabricated from patients' own extracted teeth has become possible due to the development of tooth banking procedures. The Korea Tooth Bank (KTB), established in Seoul in 2009, is one such tooth-banking facility that can procure and store teeth, and then process them into bone graft substitutes. Another is the Hospital Tooth Bank (HTB) at Seoul National University Bundang Hospital (SNUBH), established in 2010 for performing storage and grafting of auto-tooth bone grafts based on experimental and clinical research. Extracted teeth are sent to the above-mentioned facility units and then delivered back to the patients for clinical use, and thus the safety of the auto-tooth bone graft materials as well as the clinical effectiveness must be guaranteed through proper quality assurance (QA) procedures. For the purpose of this investigation, we analyzed written documents for QA at KTB, and we performed histopathologic and microbiologic examinations against the banked tooth materials at the HTB. The results suggest that the tooth banking systems at both KTB and HTB sufficiently ensure patient safety. © 2014 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved. Source

Petchdee S.,Kasetsart University | Pattanapon N.,Kasetsart University | Bootcha R.,Kasetsart University | Srivattanakul P.,Tooth Bank | Songserm T.,Kasetsart University
Cardiology (Pakistan)

Coronary artery disease is a common precursor to sudden cardiac death worldwide. Advanced symptoms usually include Myocardial Infarction (MI) due to atherosclerosis of coronary arteries. To repair or regenerate lost myocardium and coronary vasculature, stem cell transplantation is a promising therapeutic approach for the treatment of coronary heart diseases. In this study, the therapeutic effects of multipotent Stem Cells from Human Exfoliated Deciduous teeth (SHED) were examined. The 30 adult male New Zealand White rabbits underwent a left thoracotomy approach for producing chronic infarcted heart. The marginal branch of the left circumflex coronary artery was ligated over 8 weeks to produce an ischemic area of 20-25% of the Left Ventricle (LV). SHEDs were freshly prepared and 1.0 mL of 106 cells were injected to each of eight rabbits via the marginal ear vein. Echocardiography and Heart Rate Variability (HRV) were measured to reflect cardiac function. The infarcted size measurements were performed at the end of each experiment. The SHED treatment groups show significant improvement in cardiac autonomic tone and reduction in infarcted size. Dental tissue derived stem cell transplantation confirmed a restoration of the heart. The results suggest that SHED could provide an alternative selection of the precursor cells for cardiac repair. © Medwell Journals, 2014. Source

Nam H.,Dental Research Institute | Kim J.,Dental Research Institute | Park J.,Dental Research Institute | Park J.-C.,Seoul National University | And 4 more authors.
Molecules and Cells

Hertwig's epithelial root sheath/Epithelial rests of Malassez (HERS/ERM) cells are unique epithelial cells in the periodontal ligament. They remain in periodontal tissues throughout the adult life, and it is expected that their functional role is to maintain the homeostasis of the periodontium through reciprocal interactions with other periodontal cells. In this study, we investigated whether HERS/ERM cells have primitive stem cell characteristics: those of embryonic stem cells as well as of epithelial stem cells. Primary HERS/ERM cells had typical epithelial cell morphology and characteristics and they maintained for more than five passages. They expressed epithelial stem cell-related genes: ABCG2, ΔNp63, p75, EpCAM, and Bmi-1. Moreover, the expression of embryonic stem cell markers such as Oct-4, Nanog, and SSEA-4 were detected. Next, we investigated whether the expression of these stem cell markers was maintained during the sub-culture process. HERS/ERM cells showed different expression levels of these stemness genes at each passage, but their expression was maintained throughout the passages. Taken together, our data suggest that a primary culture of HERS/ERM cells contains a population of primitive stem cells that express epithelial stem cell markers and embryonic stem cell markers. Furthermore, these cell populations were maintained during the sub-culturing process in our culture conditions. Therefore, our findings suggest that there is a strong possibility of accomplishing cementum tissue engineering with HERS/ERM cells. Source

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