Peoples Hospital of Tongling
Peoples Hospital of Tongling
Mou X.-Z.,Zhejiang University |
Mou X.-Z.,Institute for Cell Based Drug Development of Zhejiang Province |
Lin J.,Zhejiang University |
Lin J.,Peoples Hospital of Tongling |
And 9 more authors.
Journal of Zhejiang University: Science B | Year: 2013
Orthotopic liver transplantation (OLT) is the only proven effective treatment for both end-stage and metabolic liver diseases. Hepatocyte transplantation is a promising alternative for OLT, but the lack of available donor livers has hampered its clinical application. Hepatocyte-like cells (HLCs) differentiated from many multi-potential stem cells can help repair damaged liver tissue. Yet almost suitable cells currently identified for human use are difficult to harvest and involve invasive procedures. Recently, a novel mesenchymal stem cell derived from human menstrual blood (MenSC) has been discovered and obtained easily and repeatedly. In this study, we examined whether the MenSCs are able to differentiate into functional HLCs in vitro. After three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor (HGF), fibroblast growth factor-4 (FGF-4), and oncostain M (OSM), cuboidal HLCs were observed, and cells also expressed hepatocyte-specific marker genes including albumin (ALB), α-fetoprotein (AFP), cytokeratin 18/19 (CK18/19), and cytochrome P450 1A1/3A4 (CYP1A1/3A4). Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis, glycogen storage, and indocyanine green (ICG) uptake. After intrasplenic transplantation into mice with 2/3 partial hepatectomy, the MenSC-derived HLCs were detected in recipient livers and expressed human ALB protein. We also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury animals. In conclusion, MenSCs may serve as an ideal, easily accessible source of material for tissue engineering and cell therapy of liver tissues. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.
Zhan S.,Peoples Hospital of Tongling |
Zhou D.,Peoples Hospital of Tongling
Pharmaceutical Care and Research | Year: 2010
Objective: To investigate and analyze the distribution of Staphylococcus aureus(SA) strains isolated from clinic and their susceptibility to antibiotics in the People's Hospital of Tongling, in order to provide a reference for rational choice of antibiotics in clinic. Methods: The distribution of microbials and the results of susceptibility test by filter paper method of SA in the hospital during 2005 to 2008 were analyzed. Results: In the isolated 294 SA strains, clinical departments of orthopedics (28.59%), burns (11.90%), and general surgery (7.48%) had high detection rates, which showed apparent characteristics of clinical distribution. Submitted specimens were mainly wound secretions (49.66%), sputum (29.59%), pus (18.50%), etc. The detection rate of methicillin-resistant Staphylococcus aureus (MRSA) was 27.21% (80/294). MRSA showed characteristics of multi-drug resistance to macrolides, quinolones and lincomycin, while methicillin-sensitive Staphylococcus aureus (MSSA) was relatively susceptible to them. All SA strains showed high drug resistance rates to β-lactams, aminoglycosides, while they showed low drug resistance rates (less than 20%) to sulfamethoxazole-trimethoprim, nitrofurantoin, rifampicin and fosfomycin. No teicoplanin, vancomycin-resistant strains were found. Conclusion: Regular epidemiological investigation of bacteria in hospital and analysis of bacterial resistance can provide guidance for clinical rational drug use.
Yuan K.X.,Peoples Hospital of Tongling |
Ling Y.,Peoples Hospital of Tongling |
Zhou F.,Peoples Hospital of Tongling |
Zhan S.H.,Peoples Hospital of Tongling
Pharmaceutical Care and Research | Year: 2013
Objective;To investigate the frequency of medication (DDDs) of antibacterials in the People's Hospital of Tongling, Anhui Province and the distribution of bacteria, and also to analyze and evaluate the composition of antibacterials used in the hospital, so as to provide evidence for rational application of drugs clinically. Methods: The medical data about the amount of antibacterials used by inpatients of the hospital in 2011 were retrieved by using the Information System of the Pharmacy Department. The utilization of antibacterials was analyzed with defined daily dose (DDD). The rationality of the composition of antibacterials was analyzed in accordance with the results of bacterial culture, drug sensitivity test and the characteristics of bacterial drug resistance. Results: The most commonly used antibacterials in the hospital were cephalosporins and compound preparation, including [3-lactamase inhibitors. Conclusion: The composition of antibacterials was basically rational in the hospital. The survey of the utilization of antibacterials could promote rational use of drugs.