Wang C.,Inner Mongolia University for Nationalities |
Li G.-R.,Inner Mongolia University for Nationalities |
Zhang Z.-Y.,Tongliao Academy of Agricultural Science |
Peng M.,Inner Mongolia University for Nationalities |
And 3 more authors.
Biochemical Systematics and Ecology
Inter-Simple Sequence Repeat (ISSR) markers were employed to analyze the genetic diversity of Ricinus communis L. in northeastern China plants. We selected ten primers that produced clear, reproducible and multiple bands for these experiments and 179 bands were obtained across 39 genotypes. Polymorphic band ratios ranged from 100% to a minimum of 78.9% with an average of 96.4% while band numbers were comprised between 13 (UBC823) and 23 (UBC856). The results obtained from UPGMA clustering dendrogram and PCoA lead to 39 distinct castor bean accessions belonging to four major groups. We found that all groups shared a common node with 66% similarity while Jaccard's similarity coefficient ranged from 0.58 to 0.92. Compatible inference was also observed from the highvalues of heterozygosity (Ht=0.3378±0.0218), Nei's genetic diversity (H=0.1765±0.2090), and Shannon's information index (I=0.4942±0.1872). In addition, our data reveal a Nei's genetic differentiation index (GST) of 0.3452 and estimated the gene flow (Nm) at 0.9482. These findings clearly suggest a genetic diversity in castor bean germplasms from various geographic origins and contribute to our understanding of breeding and conservation of castor beans. © 2013 Elsevier Ltd. Source
Li Y.,China Agricultural University |
Zheng C.,Beijing Capital International Airport Entry Exit Inspection and Quarantine Bureau |
Zhao Z.,Dalian Nationalities University |
Lu G.,Dalian Nationalities University |
And 4 more authors.
European Journal of Plant Pathology
Adonis palaestina Boiss. is one of the top three natural sources of red pigment astaxanthin, which has been used as a valuable antioxidant nutraceutical and a feed additive for salmonid fish raising. Since 2004, a blight disease causing significant damage to plants of A. palaestina in Inner Mongolia, China has occurred. The disease caused small, brown lesions on petioles and stems of the host plants. The disease initially appeared in the field as a brown necrosis on lower parts of the plant, and eventually expanded to the whole plant resulting in complete defoliation. The fungus consistently isolated from symptomatic tissues was identified as Phoma adonidicola based on morphological characteristics. Phylogenetic analysis based on LSU (Large Subunit - 28S), ITS (ITS1-5.8S-ITS2 region), and TUB (β-tubulin) showed that the P. adonidicola isolates fall in the same well-supported clade, which is closely related to Stagonosporopsis ajacis. All isolates of P. adonidicola also caused typical spots on inoculated plants, and were successfully reisolated from the symptomatic tissues. The disease of A. palaestina was proposed as spot blight. © 2013 KNPV. Source
Huang F.,Inner Mongolia University for Nationalities |
Bao C.,Tongliao Academy of Agricultural Science |
Bao C.,Inner Mongolia University for Nationalities |
Peng M.,Northeast Forestry University |
And 5 more authors.
Biotechnology and Biotechnological Equipment
The aim of this study was to examine the relationship between oil content and seed size in 32 castor accessions. The oil content and fatty acid composition were determined using the Soxhlet extraction method and capillary gas chromatography. The results indicated that the values of seed characteristics were very variable. The crude fat percentage among the accessions ranged from 36.62% to 49.19%. The content of ricinoleic acid was highest in all fatty acid compositions, from 82.3% to 88.6%. The other fatty acids appeared at a low content (less than 1%). Correlation analysis revealed that seed size was significantly positively correlated with 100-seed weight, the crude fat content and the ricinoleic acid content but showed negative correlation with the content of palmitic and arachidic acids. Hierarchical clustering of castor cultivars separated the 32 accessions into four independent clusters. The obtained results provide useful information for further research in breeding and utilization of castor oil. © 2015 The Author(s). Published by Taylor & Francis. Source
Seed characteristics and fatty acid composition of castor (Ricinus communis L.) varieties in Northeast China [Características y composición de ácidos grasos de la semilla en variedades de Ricinus communis L. en el Noreste de China]
Huang F.L.,Inner Mongolia University for Nationalities |
Zhu G.L.,Tongliao Academy of Agricultural Science |
Chen Y.S.,Inner Mongolia University for Nationalities |
Meng F.J.,Northeast Forestry University |
And 6 more authors.
Oil content and fatty acid composition were investigated on 12 castor varieties and strains by using the soxhlet extraction method and capillary gas chromatography. This was made to provide a reference and theoretical basis for castorbean breeding with high oil content, determine variability of seed compounds for breeding purposes, and broaden chemical material choices. Results revealed that crude fat percentage in seeds ranged from 18.91 to 35.84% with an average of 25.91%; the absolute content of ricinoleic by SSPS based acid varied between 171.65 g/kg and 314.03 g/kg with an average of 222.43 g/kg, and kernel crude fat percentage was between 24.28 and 46.97% with an average of 34.30%. All these study variables were highest in the 2129 strain. The percentage of ricinoleic acid in crude fat was between 83.85 to 87.62%, and the highest value was found in the zhebi4 accession. The other fatty acids appeared in small concentrations, and showed small amplitude: 1.12 to 1.61%, 1.21 to 1.61%, 3.53 to 4.80%, 5.35 to 6.38%, 0.52 to 0.79%, 0.05 to 0.08% and 0.43 to 0.55%, for palmitic, stearic, oleic, linolic, linolenic, arachidic, and arachidonic acids, respectively. One hundred seed weight was determined for each accession. One hundred seed weight ranged from 25.7 g to 34.0 g with an average of 29.9 g. There was a significant correlation between seed weight and oil content, but the correlation value was low (r=0.51). Cluster analysis by SSPS based on the content of fatty acid composition revealed that the accessions were divided into three independent clusters. These findings will clearly provide useful information for further research in breeding and utilization of castor oil. © 2015, Fund Roulo Raggio. All rights reserved. Source