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Wu J.,Tsinghua University | He Z.,Tsinghua University | Wang D.-L.,Tsinghua University | Sun F.-L.,Tsinghua University | Sun F.-L.,Tongji UniversityShanghai
Cell Cycle | Year: 2016

Microtubules play essential roles in mitosis, cell migration, and intracellular trafficking. Drugs that target microtubules have demonstrated great clinical success in cancer treatment due to their capacity to impair microtubule dynamics in both mitotic and interphase stages. In a previous report, we demonstrated that JMJD5 associated with mitotic spindle and was required for proper mitosis. However, it remains elusive whether JMJD5 could regulate the stability of cytoskeletal microtubules and whether it affects the efficacy of microtubule-targeting agents. In this study, we find that JMJD5 localizes not only to the nucleus, a fraction of it also localizes to the cytoplasm. JMJD5 depletion decreases the acetylation and detyrosination of α-tubulin, both of which are markers of microtubule stability. In addition, microtubules in JMJD5-depleted cells are more sensitive to nocodazole-induced depolymerization, whereas JMJD5 overexpression increases α-tubulin detyrosination and enhances the resistance of microtubules to nocodazole. Mechanistic studies revealed that JMJD5 regulates MAP1B protein levels and that MAP1B overexpression rescued the microtubule destabilization induced by JMJD5 depletion. Furthermore, JMJD5 depletion significantly promoted apoptosis in cancer cells treated with the microtubule-targeting anti-cancer drugs vinblastine or colchicine. Together, these findings suggest that JMJD5 is required to regulate the stability of cytoskeletal microtubules and that JMJD5 depletion increases the susceptibility of cancer cells to microtubule-destabilizing agents. © 2016 Taylor & Francis.


Zafari A.,University of MelbourneVictoria | Wei X.S.,University of MelbourneVictoria | Wei X.S.,Tongji UniversityShanghai | Xu W.,University of MelbourneVictoria | And 2 more authors.
Acta Materialia | Year: 2015

Abstract High pressure torsion was conducted to obtain nano-sized β grains in a metastable Ti-5553 alloy. Much finer grains of <50 nm were achieved, compared to >100 nm in a stable Ti-20 wt.% Mo alloy. The more effective grain refinement was attributed to stress induced martensitic transformation in the former, leading to the formation of thin α" plates which divide β grains into smaller domains. Further deformation resulted in a reverse α" to β transformation with decreasing α" sizes, generating a completely nano β grain structure at very large straining. A detailed description of the β grain refinement mechanism is provided. The reverse transformation is shown to be caused by the significantly increased free energy below a critical α" size of ∼10 nm, consistent with experimental observations. It is also calculated that extremely high energies were required for the formation of α" in nano-sized β grains, making further martensitic transformation impossible. It is concluded that the stress induced martensitic transformation and the subsequent reverse transformation are critical to producing nano-grained metastable β Ti alloys. © 2015 Acta Materialia Inc.


Sun L.,Tongji UniversityShanghai | Sun L.,Hong Kong Polytechnic University | Zhu D.,Tongji UniversityShanghai | Chan E.H.W.,Hong Kong Polytechnic University
Land Use Policy | Year: 2016

Public participation is vital for conflict management. Although much practice and best experience of environmental conflict and NIMBY conflict management come from the Western countries, the effectiveness of public participation has seldom been examined in China. Comparative studies in Shanghai and Hong Kong were conducted to examine public participation impact on environment NIMBY conflict and environmental conflict management. The results show that involved stakeholders, the degree of participation, participation approach and timing has impact. There is no public participation during the planning/project decision-making stage in both cases. In Mainland China, manipulation and therapy participation, few participation approach and late participation led negative impacts on public acceptance to NIMBY facilities. Based on the findings in the case studies, involving key stakeholders, enhancing the degree of participation, purposely participation approach and timing participation in the project lifecycle process are suggested for environment NIMBY conflict and environmental conflict management. © 2016 Elsevier Ltd


Donkers S.,Grontmij Nederland BV the Netherlands | Ledoux H.,Technical University of Delft | Zhao J.,Tongji UniversityShanghai | Stoter J.,Technical University of Delft
Transactions in GIS | Year: 2015

Although the international standard CityGML has five levels of detail (LODs), the vast majority of available models are the coarse ones (up to LOD2, i.e. block-shaped buildings with roofs). LOD3 and LOD4 models, which contain architectural details such as balconies, windows and rooms, rarely exist because, unlike coarser LODs, their construction requires several datasets that must be acquired with different technologies, and often extensive manual work is needed. In this article we investigate an alternative to obtaining CityGML LOD3 models: the automatic conversion from already existing architectural models (stored in the IFC format). Existing conversion algorithms mostly focus on the semantic mappings and convert all the geometries, which yields CityGML models having poor usability in practice (spatial analysis, for instance, is not possible). We present a conversion algorithm that accurately applies the correct semantics from IFC models and that constructs valid CityGML LOD3 buildings by performing a series of geometric operations in 3D. We have implemented our algorithm and we demonstrate its effectiveness with several real-world datasets. We also propose specific improvements to both standards to foster their integration in the future. © 2015 John Wiley & Sons Ltd.


Sun D.,Tongji UniversityShanghai | Lin T.,Tongji UniversityShanghai | Zhu X.,Tongji UniversityShanghai | Cao L.,Tongji UniversityShanghai
Construction and Building Materials | Year: 2015

Abstract Self-healing, as a very valuable characteristic, should be considered when selecting and designing constitutions of asphalt materials. It is necessary to develop a fundamental and universal healing evaluation indication based on the healing mechanism. However, most existing healing evaluation indication lacks physical meaning and hardly can predict the time-dependent healing process under different conditions. In this paper, the formulation of activation energy as a self-healing indication is developed based on the Arrhenius law. The procedure to get the activation energy of different asphalt mastic is put forward. Nine samples of different asphalt mastic are prepared. Then, the fatigue-rest-fatigue tests are employed to get the activation energy of nine asphalt mastic. Finally, the potential of the activation energy as the healing evaluation index for asphalt mastic is studied, and the effect of type of asphalt and filler, asphalt-filler ratio, damage degree on the value of activation energy is discussed. It is found that the healing activation energy can obviously distinguish the healing ability of different asphalt mastic. Healing activation energy represents the minimum energy required for the time-dependent strength gain and reflects the time-dependent strength gain rate, also can rank the time-dependent healing capacity of different asphalt mastic. © 2015 Elsevier Ltd.


Xu J.,Tongji UniversityShanghai | Sun X.,Tongji UniversityShanghai
International Journal of Mechanical Sciences | Year: 2015

Abstract The potential beneficial performance of a Multi-Direction Quasi-Zero-Stiffness vibration isolator (MDQZS-VI) with linear time-delayed active control is investigated in this study. From the analysis of vibration property such as natural frequency, it is found that the isolation effectiveness of the MDQZS-VI for different directions can be adjusted respectively. The vertical direction natural frequency and damping property of the MDQZS-VI are dependent on structural parameters while the horizontal direction natural frequency and damping coefficient are related to the active control parameters. Moreover, bifurcation analysis shows that the values of control strength and time-delay have effect on the stability of horizontal vibration. By solving the displacement transmissibility for multi-direction, it is shown that structural parameters and active control coefficients could be designed deliberately so that natural frequencies are reduced and nonlinear damping coefficients are increased which is helpful for vibration suppression. The results provide a useful insight into the analysis and design of multi-direction QZS vibration isolator by exploiting potential nonlinear benefits of the time delay in vibration active control. © 2015 Elsevier Ltd.


Li J.,Glaxosmithkline | Ma J.,Glaxosmithkline | Ma J.,Tongji UniversityShanghai | Meng G.,Glaxosmithkline | And 13 more authors.
Stem Cell Research | Year: 2016

Neural stem cells and progenitor cells (NPCs) are increasingly appreciated to hold great promise for regenerative medicine to treat CNS injuries and neurodegenerative diseases. However, evidence for effective stimulation of neuronal production from endogenous or transplanted NPCs for neuron replacement with small molecules remains limited. To identify novel chemical entities/targets for neurogenesis, we had established a NPC phenotypic screen assay and validated it using known small-molecule neurogenesis inducers. Through screening small molecule libraries with annotated targets, we identified BET bromodomain inhibition as a novel mechanism for enhancing neurogenesis. BET bromodomain proteins, Brd2, Brd3, and Brd4 were found to be downregulated in NPCs upon differentiation, while their levels remain unaltered in proliferating NPCs. Consistent with the pharmacological study using bromodomain selective inhibitor (+)-JQ-1, knockdown of each BET protein resulted in an increase in the number of neurons with simultaneous reduction in both astrocytes and oligodendrocytes. Gene expression profiling analysis demonstrated that BET bromodomain inhibition induced a broad but specific transcription program enhancing directed differentiation of NPCs into neurons while suppressing cell cycle progression and gliogenesis. Together, these results highlight a crucial role of BET proteins as epigenetic regulators in NPC development and suggest a therapeutic potential of BET inhibitors in treating brain injuries and neurodegenerative diseases. © 2016 The Authors


Zhao X.,Tongji UniversityShanghai | Wang H.,Tongji UniversityShanghai | Hu X.,Tongji UniversityShanghai | Liu J.,Tongji UniversityShanghai | Jiang G.,Tongji UniversityShanghai
International Journal of Clinical and Experimental Medicine | Year: 2014

We present the first report of a rare case of typical carcinoid tumor of the lung in a patient with complete situs inversus. The patient was a 65-year-old woman with the chief complaint of productive and mild chest pain. Chest X-ray and computed tomography (CT) scans of the thorax showed a mirror image of the organs and vessels and revealed a tumor 2.5 cm in diameter in the left middle lobe. The patient underwent resection of the left middle lobe through posterolateral incision. Postoperative pathological examination returned a diagnosis of typical carcinoid tumor. The patient made an uneventful recovery. This case highlights the importance of considering malignant tumors in pulmonary nodules in patients with complete situs inversus. © 2014, International Journal of Clinical and Experimental Medicine. All rights reserved.


PubMed | Tongji UniversityShanghai and Tongji University
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2016

To investigate the morphological changes in various tissues of rabbits receiving sciatic nerve block with 0.2% ropivacaine for 48 h.Twenty healthy were randomly assigned to normal saline group (N group) and ropivacaine group (R group). The right sciatic nerve was exposed, and a nerve-blocking trocar cannula embedded. Animals received an injection of 0.5% ropivacaine hydrochloride at a dose of 0.75 ml/kg. Rabbit was then connected to an infusion pump containing 50 ml of normal saline in N group, or to a infusion pump containing 0.2% ropivacaine hydrochloride in R group at 0.25 ml/kgh-1.In both R group and N group, a small number of nerve cells exhibited pyknotic degeneration. More nerve cells with pyknotic degeneration were found in R group than in N group (P<0.001). At 48 h after surgery, there was a significant correlation between the abnormality of right hind limb and the degree of edema in sciatic nerve (P<0.01).Pyknotic degeneration of sciatic nerve increased after an infusion of 0.2% ropivacaine hydrochloride for 48 h, suggesting the neurotoxicity of ropivacaine. An infusion of 0.2% ropivacaine hydrochloride for 48 h may cause necrosis of skeletal muscle cells. The sciatic nerve edema would greatly affect the hindlimb motor while both pyknotic degeneration of sciatic nerve and skeletal muscle have little influence on the hindlimb movement. After an infusion of 0.2% ropivacaine hydrochloride for 48 h, the morphology of right atrium and brain tissues around the ventriculus tertius and medulla oblongata remained unchanged.

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