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Shanghai, China

Mei X.,Shanghai First Peoples Hospital | Shi W.,Shanghai First Peoples Hospital | Piao Y.,Tongji Hospital
Photodermatology Photoimmunology and Photomedicine | Year: 2013

Summary: Background: The success rates of conventional treatments to acne vulgaris are limited because of intolerance and resistance. Photodynamic therapy with topical 5-aminolevulinic acid (ALA) and red light has been introduced. However, the side effects especially pigmentation are common. Objective: To study the efficacy and safety of ALA-photodynamic therapy (PDT) with 420-950nm intense pulsed light (IPL) in Chinese patients with acne vulgaris. Methods: Forty-one patients with moderate to severe facial acne were randomly assigned to ALA-IPL-PDT group and IPL group. Ten percent topical ALA was applied to patients in the ALA-IPL-PDT group, while placeboes were applied to patients in the IPL group. After 1h occlusion, all patients were illuminated with 420-950nm IPL. The patients in both groups had four treatment sessions with 1-week intervals. One week after each treatment and 4, 8, and 12 weeks after four sessions, acne lesion counts and adverse events were observed. Results: Twelve weeks after treatments, mean reductions of global lesion counts of ALA-IPL-PDT group and IPL group were 75.2% and 51.0%, respectively. Mean reductions of inflammatory and non-inflammatory lesion counts in ALA-IPL-PDT group were (83.6±4.1)% and (57.5±6.8)%, respectively. No severe adverse events were observed. Conclusion: ALA-IPL-PDT is an effective treatment for moderate to severe acne vulgaris, and side effects are mild and reversible. © 2013 John Wiley & Sons A/S. Source

Zhang X.,Emory University | Gu L.,Emory University | Li J.,Emory University | Shah N.,Emory University | And 4 more authors.
Cancer Research | Year: 2010

Berberine, a natural product derived from a plant used in Chinese herbal medicine, is reported to exhibit anticancer effects; however, its mechanism of action is not clearly defined. Herein, we demonstrate that berberine induces apoptosis in acute lymphoblastic leukemia (ALL) cells by downregulating the MDM2 oncoprotein. The proapoptotic effects of berberine were closely associated with both the MDM2 expression levels and p53 status of a set of ALL cell lines. The most potent apoptosis was induced by berberine in ALL cells with both MDM2 overexpression and a wild-type (wt)-p53, whereas no proapoptotic effect was detected in ALL cells that were negative for MDM2 and wt-p53. In contrast to the conventional chemotherapeutic drug doxorubicin, which induces p53 activation and a subsequent upregulation of MDM2, berberine strongly induced persistent downregulation of MDM2 followed by a steady-state activation of p53. We discovered that downregulation of MDM2 in ALL cells by berberine occurred at a posttranslational level through modulation of death domain-associated protein (DAXX), which disrupted the MDM2-DAXX-HAUSP interactions and thereby promoted MDM2 self-ubiquitination and degradation. Given that MDM2-overexpressing cancer cells are commonly chemoresistant, our findings suggest that this naturally derived agent may have a highly useful role in the treatment of cancer patients with refractory disease. ©2010 AACR. Source

Wang Y.,Tongji Medical College | Yan L.,Tongji Hospital | Jin Z.,Tongji Medical College | Xin X.,Tongji Medical College
PLoS ONE | Year: 2011

Background: Sleeve gastrectomy (SG) has emerged recently as a stand-alone bariatric procedure to treat morbid obesity and enhance glucose homeostasis. The aim of the study was to evaluate its effects in neonatally streptozotocin (STZ)- induced diabetic rats (n-STZ diabetic rats). Methodology and Principal Findings: To induce diabetes, STZ (90 mg/kg) was administered intraperitoneally to 2-day-old male pups. When 12 weeks old, diabetic rats were randomized into sleeve operation group (SLG, n = 6) and sham operation group (SOG, n = 6). Body weights were monitored weekly, and daily consumption of water and food were followed for eight consecutive weeks postoperatively. Serum glucose levels were measured periodically at the 4th and 8th week after surgery. Insulin, ghrelin, glucose-dependent insulinotropic polypeptide (GIP) and Glucagon-like peptide-1 (GLP-1) levels were assayed at the end of the study. Our data showed that SLG rats exhibited significantly lower body weight gain in addition to reduced food and water intakes postoperatively compared to their sham-operation counterparts. However, resolution of diabetes was not observed in our study. Correspondingly, there were no significant differences between SOG rats and SLG rats in glucose metabolism-associated hormones, including insulin, GIP and GLP-1. In contrast, ghrelin level significantly decreased (P,0.01) in SLG group (58.01±3.75 pg/ml) after SG surgery compared to SOG group (76.36±3.51 pg/ml). Conclusions: These observations strongly suggest that SG is effective in controlling body weight. However, SG did not achieve resolution or improvement of diabetes in n-STZ diabetic rats. © 2011 Bai et al. Source

Juric M.,Hannover Medical School | Xiao F.,Tongji Hospital | Amasheh S.,Institute of Clinical Physiology | May O.,Hannover Medical School | And 5 more authors.
Inflammatory Bowel Diseases | Year: 2013

Background: Bicarbonate loss into the lumen occurs during intestinal inflammation in different species. However, candidate pathways like CFTR or DRA are inhibited in the inflamed gut. This study addressed the question whether and how inflammation-associated increased intestinal permeability may result in epithelial HCO- 3 loss. Methods: Murine proximal colon was studied because it does not express functional DRA but is inflamed in the tumor necrosis factor a overexpressing mouse model (TNFδARE). Luminal alkalization, 3H-mannitol fluxes, impedance spectroscopy, and dilution potentials were measured in Ussing chambers, whereas expression and localization of tight junction-associated proteins were analyzed by Western blots and immunohistochemistry. Results: Luminal alkalization rates and 3H-mannitol fluxes were increased in TNF+/δARE proximal colon, whereas forskolin-stimulated Isc was not altered. Epithelial resistance was reduced, but subepithelial resistance increased. The epithelial lining was intact, and enterocyte apoptosis rate was not increased despite massively increased Th1 cytokine levels and lymphoplasmacellular infiltration. Measurement of dilution potentials suggested a loss of cation selectivity with increased anion permeability. Western analysis revealed a downregulation of occludin expression and an upregulation of both claudin-2 and claudin-5, with no change in ZO-1, E-cadherin, claudin-4, and claudin-8. Immunohistochemistry suggested correct occludin localization but reduced tight junction density in TNF +/δARE surface epithelium. Conclusions: Inflammation during TNF-α overexpression leads to increased epithelial permeability in murine proximal colon, decreased tight junctional cation selectivity, and increased HCO- 3 loss into the lumen. Inflammation-associated colonic HCO- 3loss may occur through leaky tight junctions rather than through HCO23 secreting ion transporters. Copyright © 2013 Crohn's & Colitis Foundation of America, Inc. Source

Liang W.,Tongji Hospital
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2012

To investigate the apoptosis effect of diffuse large B-cell lymphoma cell line (DLBCL) SUDHL-4 induced by IL-21 and its related mechanism. SUDHL-4 cells were treated with IL-21 at different concentration (1000 ng/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml) for 24 h, 48 h, 72 h, respectively. The inhibitory rate of cell proliferation was detected by CCK-8 assay. The cell growth curves were drawn and half inhibitory concentration (IC(50)) values were calculated. The cell apoptosis were detected by flow cytometry (FCM), the expression of the caspase-9, caspase-3, cleaved caspase-3, Bcl-2, Bcl-XL, Bid, Bax and c-myc protein in SUDHL-4 cells treated with IL-21 by western blot, the mRNA expression of Bcl-2, Bcl-XL, Bid, Bax, c-myc by Survivin gene with RT-PCR. IL-21 markedly inhibited SUDHL-4 cell growth in a time- and dose-dependent manner. The 48 hIC(50) was 140.9ng/ml; The FCM showed that the apoptosis proportion of SUDHL-4 cells treated with 100 ng/ml of IL-21 apoptosis (AnnexinV-FITC(+) positive cells) gradually increased (48 h: 19.7 ± 2.3%). The protein expression of caspase-9, caspase-3, Bcl-2 and Bcl-XL decreased in a time-dependent manner. The Bax and c-myc protein markedly increased, but the Bid protein level did not change. IL-21 up regulated c-myc and Bax gene expression, however down regulated Bcl-2 and BCL-XL gene expression, but the gene expression of Bid and Survivin hadn't been changed significantly. IL-21 can inhibit proliferation and induce apoptosis of SUDHL-4 cell. The mechanism may involve in endogenous mitochondrial pathway mediated by the c-myc and the Bcl-2 genes. Source

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