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Saprina T.V.,Siberian State Medical University | Timokhina E.S.,Siberian State Medical University | Goncharevich O.K.,Tomsk Regional Perinatal Center | Budeeva S.V.,Tomsk Regional Perinatal Center | And 4 more authors.
Diabetes Mellitus | Year: 2016

Background: Enteropancreatic hormonal system disorder is a possible reason for β-cell dysfunction and carbohydrate metabolism disorder among pregnant women. However, no information is available about the state of enteroinsulin hormones [glucagon, glucose-dependent insulinotropic peptide (GIP), glucagon-like peptide1 (GLP-1) and GLP-2] during pregnancy. The role of enteroinsulin hormones in the development of carbohydrate metabolism disorder during pregnancy is poorly understood. Aim: To quantify and compare incretin hormone secretion in groups of pregnant women with and without gestational diabetes mellitus (GDM). Materials and methods: The study included 80 patients, 50 of whom had GDM, and the control group consisted of 30 pregnant women without GDM. All patients underwent an oral glucose tolerance test; glycated haemoglobin (HbA1c) estimation; ferritin, transferrin, basal and postprandial glucagon estimation; GLP-1 and GLP-2 estimation. Results: Basal glucagon and GLP-1 levels were significantly higher (p <0.05) in the group of women with GDM than in the control group. The most significant differences in GLP-1, basal and postprandial glucagon levels were observed during the first trimester of pregnancy. Conclusion: High GLP-1 levels in the group of women with GDM may reflect a state of 'incretin resistance', which is similar to hyperinsulinemia in the early stages of type 2 diabetes mellitus. © Russian Association of Endocrinologists, 2016. Source

Pankova O.V.,Tomsk Cancer Research Institute | Denisov E.V.,Tomsk State University | Ponomaryova A.A.,Tomsk Polytechnic University | Gerashchenko T.S.,Tomsk State University | And 2 more authors.
Tumor Biology | Year: 2015

Recurrences occur in 30 % of lung cancer patients after radical therapy; however, known prognostic factors are not always effective. In this study, we investigated whether the frequency of squamous non-small cell lung cancer (NSCLC) recurrence depends on the presence of reactive lesions in tumor-adjacent bronchial epithelium. Specimens of adjacent lung tissue from 104 patients with squamous NSCLC were used for the determination of basal cell hyperplasia (BCH) and squamous metaplasia (SM) and for the analysis of the expression of Ki-67, p53, Bcl-2, and CD138. We found that recurrence was observed in 36.7 % of patients with BCH combined with SM (BCH + SM+) in the same bronchus, compared with 1.8 % in patients with isolated BCH (BCH + SM−; odds ratio (OR) 31.26, 95 % confidence interval (CI) 3.77–258.60; p = 0.00002). The percentage of Ki-67-positive cells was significantly higher in BCH + SM+ than in BCH + SM− (34.9 vs. 18.3 %; effect size 2.86, 95 % CI 2.23–3.47; p = 0.003). P53 expression was also more significant in BCH + SM+ than in BCH + SM− (14.4 vs. 9.6 %; effect size 1.22, 95 % CI 0.69–1.76; p = 0.0008). In contrast, CD138 expression was lower in BCH + SM+ than in BCH + SM− (21.8 vs. 38.5 %; effect size −6.26, 95 % CI −7.31 to −5.22; p = 0.003). Based on our results, we concluded that the co-presence of reactive bronchial lesions is associated with the development of recurrent squamous NSCLC and may be a negative prognostic indicator. In addition, significant differences in Ki-67, p53, and CD138 expression exist between isolated BCH and BCH combined with SM that probably reflect part of biological differences, which could relate to the mechanism of lung cancer recurrence. © 2015 International Society of Oncology and BioMarkers (ISOBM) Source

Kharchenko S.S.,Tomsk State University of Control Systems and Radioelectronics | Mescheryakov R.V.,Tomsk State University of Control Systems and Radioelectronics | Volf D.A.,Tomsk State University of Control Systems and Radioelectronics | Balatskaya L.N.,Tomsk Cancer Research Institute | Choinzonov E.L.,Tomsk Cancer Research Institute
Proceedings - 2015 International Conference on Biomedical Engineering and Computational Technologies, SIBIRCON 2015 | Year: 2015

This article describes the speech signal fundamental frequency evaluation algorithm in view of speech formation and speech perception features connected with human anatomy and physiology, using mathematical apparatus of singular spectrum analysis for speech signal processing, for further implementation of the speech signal fundamental frequency evaluation subsystem, designed for speech rehabilitation software calculation module for cancer patients after larynx resection, used in patients rehabilitation trainings process after their full or partial loss of sonorous speech arising from laryngectomy. The researcher's results of functional testing are described. Also the results of initial testing on a group of healthy speakers are presented. © 2015 IEEE. Source

Tashireva L.A.,Tomsk Cancer Research Institute | Denisov E.V.,Tomsk State University | Savelieva O.E.,Tomsk State University | Gerashchenko T.S.,Tomsk State University | And 2 more authors.
Biopolymers and Cell | Year: 2015

Aim. To investigate the expression of the TGFB1, TNF, CSF1, CSF2, VEGFA and HIF1A genes in the patients with invasive breast carcinoma of no special type considering the intratumoral morphological heterogeneity. Methods. The technology of laser capture microdissection PALM was used to isolate five types of morphological tumor structures from three patients with invasive carcinoma of no special type (IC NST), luminal A subtype, T1-2NxMx. The level of expression of the cytokine (TNF), growth factors (TGFB1, CSF1, CSF2, VEGFA) and the HIF1A genes was assessed in the samples obtained using quantitative real-time PCR. Results. The study demonstrated the absence of the expression of the growth factor CSF2 gene in tumor cells of IC NST, and the expression of the CSF1 gene, independent from the metastasis status and tumor structure type. The prevalence of the expression of the VEGFA and TGFB1 genes was revealed in the alveolar and solid structures along with the rare expression of the TNF gene. Conclusions. The expression of pre-metastatic niche genes in the tumors of patients with IC NST is heterogeneous. The hypoxia-mediated change in the cytokine gene expression may be expected in the alveolar and solid structures, which ultimately results in the formation of microenvironment, facilitating tumor growth and the formation of tumor metastatic potential. © 2015 L. A. Tashireva et al. Source

Kaigorodova E.V.,Siberian State Medical University | Zavyalova M.V.,Siberian State Medical University | Bogatyuk M.V.,Tomsk Cancer Research Institute | Tarabanovskaya N.A.,Tomsk Cancer Research Institute | And 2 more authors.
Cancer Biomarkers | Year: 2015

BACKGROUND: Heat shock protein beta-1 (HspB1) is a chaperone of the sHsp (small heat shock protein). The common functions of sHsps are chaperone activity, inhibition of apoptosis, regulation of cell development, and cell differentiation, take part in signal transduction. OBJECTIVE: To study the intracellular localization of phosphorylated features and non-phosphorylated forms of HspB1 in primary breast cancer cells and to evaluate their relationship with regional lymphatic metastasis. Material and Methods: Tumor biopsies of breast tissue were collected from 100 patients with a confirmed diagnosis of invasive carcinoma, nonspecific type, between the ages of 31-80 years. Immunohistochemistry was used to determine the intracellular localization of phosphorylated and non-phosphorylated forms of HspB1. RESULTS: The result of this study showed that biopsies from patients with lymph node metastasis exhibited significantly higher levels of phosphorylated forms of HspB1 in the nucleus and cytoplasm compared with the group without lymph node metastasis. Analysis showed that the expression of phosphorylated forms of the chaperone HspB1 correlates with the amount and percentage of lymph node metastases affected. CONCLUSION: The nuclear expression of phosphorylated and non-phosphorylated forms of the chaperone HspB1 is a marker of tumor cells associated with lymphatic metastasis of breast cancer. © 2015 - IOS Press and the authors. All rights reserved. Source

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