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Chiyoda-ku, Japan

Tokyo Kasei Gakuin University is a private university in Chiyoda, Tokyo, Japan, established in 1963. The predecessor of the school was founded in 1925.Though the two share the same historical roots , this school and Tokyo Kasei-Gakuin Junior College are distinct institutions.In the 1990s, the Tsukuba Gakuin University was a women's university named as a branch of the Tokyo Kasei Gakuin. Wikipedia.

Takashima M.,Japan National Institute of Advanced Industrial Science and Technology | Nara K.,Tokyo Kasei-Gakuin University | Niki E.,Japan National Institute of Advanced Industrial Science and Technology | Yoshida Y.,Japan National Institute of Advanced Industrial Science and Technology | And 4 more authors.
Food Chemistry | Year: 2013

Groundnut (Apios americana Medik) contains a novel isoflavone, genistein-7-O-gentiobioside. In the present study, we examined the biological activities of an alcohol extract of groundnut containing genistein-7-O- gentiobioside as the main component. Although the groundnut extract by itself did not show antioxidative activity, it drove the antioxidative system in cells. Pretreatment of human breast carcinoma MCF-7 cells for 24 h with the groundnut extract and soybean isoflavone increased gene expression of heme oxygenase-1 (HO-1), a major antioxidative stress enzyme. These groundnut extract-treated cells showed antioxidative activity against free radicals derived from a radical initiator. Pretreatment of cells with 100 μg/mL groundnut extract prevented the depletion of glutathione by the radical initiator; however, treatment with 100 μg/mL of soybean isoflavone injured the cell membrane, indicating that glutathione might be released to the extracellular environment. These results suggest that the groundnut extract had isoflavone-like activity. Like soybean, groundnuts are a good source of isoflavones. © 2012 Elsevier Ltd. All rights reserved.

Araki S.,University of Occupational and Environmental Health Japan | Yamamoto Y.,University of Occupational and Environmental Health Japan | Dobashi K.,Showa University | Asayama K.,Tokyo Kasei-Gakuin University | Kusuhara K.,University of Occupational and Environmental Health Japan
Obesity Research and Clinical Practice | Year: 2014

Background: Brain-derived neurotrophic factor (BDNF) plays important roles in the central regulation of food intake and body weight control. However, little is known about the role of BDNF in childhood obesity. Objective: To investigate the relationship between plasma levels of BDNF and anthropometric factors, metabolic derangements due to obesity, adipocytokine levels and birth weight in obese Japanese children. Subjects and methods: Sixty-six obese Japanese children aged from 5 to 15 years old were enrolled. The age-matched control group consisted of 32 non-obese healthy children. The plasma levels of BDNF and adipocytokines (leptin and adiponectin) were assayed using ELISA techniques. Results: The mean BMI Z-scores were -0.67, +2.15 and +3.39 for the non-obese control children, obese (BMI ≥ 90th percentile, <99th percentile) and morbidly obese (BMI ≥ 99th percentile), respectively. The plasma levels of BDNF were significantly decreased in the morbidly obese children compared with the levels in the obese and non-obese control children (507 ± 33 pg/ml vs. 626 ± 46 pg/ml, 621 ± 35 pg/ml, p < 0.05). Univariate linear regression analysis showed that the plasma level of BDNF was positively correlated with birth weight (r = 0.264, p < 0.05) and inversely correlated with the BMI Z-score (r = -0.314, p < 0.05). Multivariate forward stepwise linear regression analysis revealed that the birth weight and BMI Z-score are independent predictors of the plasma BDNF level. Conclusion: The plasma level of BDNF, which is decreased in morbidly obese children, is associated with birth weight and the BMI Z-score. Our results suggest that BDNF may play important roles in the development and pathophysiology of childhood obesity. © 2012 Asian Oceanian Association for the Study of Obesity.

Itoh R.,Tokyo Kasei-Gakuin University
Current Medicinal Chemistry | Year: 2013

Cytosolic 5'-nucleotidase II (cN-II) is an intracellular 5'-nucleotidase characterized by substrate specificity. It preferentially hydrolyzes 6-hydroxypurine nucleotides such as IMP and GMP over AMP or UMP. cN-II is allosterically activated by ATP and inhibited by inorganic phosphate. It also has phosphotransferase activity and transfers phosphate moieties from IMP or GMP to nonphysiological nucleoside analogues used to treat some viral infections or malignancies. The cN-II gene has a strikingly conserved primary structure from humans to nematodes and its activity has been detected in various animals including snails. Its activity is highest in the livers of birds, crocodiles, lizards and snakes. The activity in chicken liver increases 2-fold by feeding a high-protein diet. These results suggest that cN-II participates, through IMP dephosphorylation, in production of uric acid as the main end product of aminonitrogen in these animals. Some studies suggest that cN-II participates in dephosphorylation of IMP accumulated in cells of some tissues to diffusible inosine for reutilization by other tissues. It has also been proposed that cN-II, together with purine nucleoside phosphorylase and hypoxanthine- guanine phosphoribosyltransferase, constitutes the "oxypurine cycle", thus regulating intracellular phosphoribosyl pyrophosphate (PRPP) concentrations. As for intracellular dephosphorylation of AMP, another intracellular 5'- nucleotidase, cN-I, is supposed to participate, because it hydrolyzes AMP more preferentially than IMP or GMP. However, for the tissues, in which the expression of cN-I is very low or undetectable, e.g. liver or brain tissues, results have been obtained that suggest the participation of cN-II in intracellular dephosphorylation of AMP. © 2013 Bentham Science Publishers.

Nara K.,Tokyo Kasei-Gakuin University | Nihei K.-I.,Utsunomiya University | Ogasawara Y.,Hirosaki University | Koga H.,Calbee Foods Co. | Kato Y.,Hirosaki University
Food Chemistry | Year: 2011

In the present HPLC-based analysis of the isoflavone profile of groundnut (Apios americana Medik) tubers, we identified a major peak that did not correspond to any known isoflavones. A water extract of groundnut tubers prepared at 4 °C showed the major and genistin peaks, whereas that prepared at 45 °C did not yield these peaks but showed a peak for genistein on HPLC analysis. When the extract was treated with β-glucosidase after the inactivation of endogenous enzymes, the major peak decreased over time and the genistin peak also decreased after a transient increase, changes which were accompanied by the appearance of a genistein peak. The HPLC elution pattern of the β-glucosidase-treated extract was markedly similar to that observed for the extract prepared at 45 °C. The major peak was then isolated and purified through HP-20 and ODS columns, and the chemical structure of the resultant component was analysed and identified as genistein-7-O-genitiobioside. The component was degraded by β-glucosidase to produce genistein, suggesting that the groundnut isoflavone is converted to genistein, similarly to soybean isoflavones, by enterobacterial β-glucosidase, a deglycosylation process which has a significant impact on the absorption efficiency and bioactivity of isoflavones. The genistein-7-O-genitiobioside content of groundnut tubers ranged from 106.2 to 352.5. mg/100. g fresh weight, depending on their cultivation area. Additionally, genistein-7-O-genitiobioside showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity in a concentration-dependent manner, implying its potential as an antioxidative component. Soybeans have been recognised as the major or only food source of isoflavones; however, the present study suggests that groundnut tubers can serve as potential functional foods that provide a sufficient amount of isoflavones and significant health benefits. © 2010.

Unno T.,Tokyo Kasei-Gakuin University | Hisada T.,TechnoSuruga Laboratory Co. | Takahashi S.,TechnoSuruga Laboratory Co.
Journal of Agricultural and Food Chemistry | Year: 2015

There has been particular interest in the prebiotic-like effects of commonly consumed polyphenols. This study aimed to evaluate the effects of hesperidin (HD) and its aglycone hesperetin (HT), major flavonoids in citrus fruits, on the structure and activity of gut microbiota in rats. Rats ingested an assigned diet (a control diet, a 0.5% HT diet, or a 1.0% HD diet) for 3 weeks. Terminal restriction fragment length polymorphism analysis revealed that the proportion of Clostridium subcluster XIVa in the feces collected at the third week of feeding was significantly reduced by the HT diet: 19.8 ± 4.3% for the control diet versus 5.3 ± 1.5% for the HT diet (P < 0.01). There was a significant difference in the cecal pool of short-chain fatty acids (SCFA), the sum of acetic, propionic, and butyric acids, between the control diet (212 ± 71 μmol) and the HT diet (310 ± 51 μmol) (P < 0.05), whereas the HD diet exhibited no effects (245 ± 51 μmol). Interestingly, dietary HT resulted in a significant increase in the excretion of starch in the feces. HT, but not HD, might reduce starch digestion, and parts of undigested starch were utilized to produce SCFA by microbial fermentation in the large intestine. © 2015 American Chemical Society.

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