Tokai Pickling Co.

Toyohashi, Japan

Tokai Pickling Co.

Toyohashi, Japan
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Ono H.,Tokai Pickling Co. | Nishio S.,Tokai Pickling Co. | Tsurii J.,Tokai Pickling Co. | Kawamoto T.,Tokai Pickling Co. | And 2 more authors.
Journal of Bioscience and Bioengineering | Year: 2014

Nukadoko is a fermented rice bran mash traditionally used for pickling vegetables in Japan. To date, the production of both homemade and commercial nukadoko depends on natural fermentation without using starter cultures. Here, we monitored chemical and microbiological changes in the initial batch fermentation of nukadoko. Nukadoko samples were prepared by spontaneous fermentation of four different brands of rice bran, and microbiome dynamics were analyzed for 2 months. In the first week, non-Lactobacillales lactic acid bacteria (LAB) species, which differed among the samples, grew proportionally to pH decrease and lactate increase. Thereafter, Lactobacillus plantarum started growing and consumed residual sugars, causing further lactate increase in nukadoko. Finally, microbial communities in all tested nukadoko samples were dominated by L.plantarum. Taken together, our results suggest that the mixture of the fast-growing LAB species and slow-growing L.plantarum may be used as a suitable starter culture to promote the initial fermentation of nukadoko. © 2014 The Society for Biotechnology, Japan.


Mu F.,Kyushu University | Masuda Y.,Kyushu University | Zendo T.,Kyushu University | Ono H.,Tokai Pickling Co. | And 4 more authors.
Journal of Bioscience and Bioengineering | Year: 2014

Biological functions of a DUF95 superfamily protein in the biosynthesis gene cluster of a novel circular bacteriocin, leucocyclicin Q (LcyQ), were characterized in this paper. Sequence analysis and database search of the regions flanking the LcyQ structural gene lcyQ revealed four open reading frames (lcyR, lcyB, lcyC, and lcyD) related to bacteriocin biosynthesis. LcyD shares some similarity to the DUF95 superfamily proteins, often found in the biosynthetic gene clusters of circular bacteriocins. Mass spectrometry analysis showed accumulation of active mature LcyQ inside lcyD knockout cells. Heterologous expression of lcyD demonstrated that it confers robust immunity against LcyQ. Peptide release/binding assay revealed that the immunity could be attributed to the secretion of LcyQ to the cell exterior. Thus, the DUF95 superfamily protein has a dual function in the biosynthesis of LcyQ, as an immunity-associated transporter and as a secretion-aiding agent. Accumulation of mature LcyQ inside the cell in lcyD knockout strains, further implied that cyclization occurs within the cell. To the best of our knowledge, this is the first report on LcyQ cyclization inside the cell and the dual role of a DUF95 superfamily protein in circular bacteriocin biosynthesis. © 2013 The Society for Biotechnology, Japan.


Mizuno T.,Mie University | Nakagawa M.,Mie University | Nakagawa M.,Ajinomoto Co. | Ono H.,Mie University | And 4 more authors.
Plant Biotechnology | Year: 2010

A multidrug resistance associated protein homolog gene, named FeABCCl, was isolated from a commercial brand of common buckwheat that has high lead tolerance and accumulation ability. The deduced amino acid sequence of FeABCCl had 64% identity with AtMRP3 (AtABCC3) of Arabidopsis thaliana, and was predicted as integral membrane protein with 12 transmembrane domains. FeABCC1 expression was induced in the shoot of common buckwheat treated with lead. The yeast transformed with FeABCCl showed a marked increasing in lead tolerance with the gene expression, and accumulated more lead compared to control. Not similar to AtMRP3, FeABCC1 expression did not effect to cadmium tolerance in yeast, and inhibition of glutathione synthesis by buthionine sulfoximine was not effect lead tolerance. These data suggests that FeABCC1 has some role for lead detoxyfication in common buckwheat independently to glutathione chelating. © 2010 The Japanese Society for Plant Cell and Molecular Biology.


Tirawat D.,Kyushu University | Noma S.,Kyushu University | Kunimoto H.,Kyushu University | Tameda S.,Tokai Pickling Co. | And 3 more authors.
International Food Research Journal | Year: 2013

Decontamination effect of rapid hygrothermal pasteurization using saturated water vapor (RHP) treatment on Chinese cabbage was compared with that of sodium hypochlorite (NaClO) solution. RHP treatment was performed by passing Chinese cabbage sample through a processing chamber saturated with water vapor. NaClO treatment followed by RHP treatment was also performed. RHP treatment showed greater or equal decontamination effect compared to NaClO solution on mesophilic bacteria, coliform bacteria, lactic acid bacteria and yeast. NaClO followed by RHP treatment showed higher decontamination effect compared to RHP treatment or NaClO solution only. No significant decontamination effect on bacterial spores was observed after the all treatments tested. The cutting of Chinese cabbage to smaller size resulted in increasing the effect of RHP treatment. On the other hand, wetting the surface of Chinese cabbage by water reduced the effect of RHP treatment.


Satsukawa M.,Tokai Pickling Co. | Kawamoto T.,Tokai Pickling Co. | Yamanaka N.,Chubu University | Cha B.-Y.,Chubu University | And 2 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2016

Gac (Momordica cochinchinensis) aril is a nutritionally superior food; however, no research has been conducted on its health functions. Therefore, we examined whether gac aril contributes to lipid metabolism in rats fed a highfat diet containing a freeze-dried powder of gac aril for 4 weeks. At the end of the feeding period, the group fed a high-fat diet containing gac aril showed significantly decreased triglyceride and cholesterol levels in the liver, and increased blood high-density lipoprotein-cholesterol compared to the high-fat diet group. Furthermore, there was a significant increase in fecal cholesterol levels in the gac diet group compared to the high-fat diet group. These results suggest that the consumption of gac aril promotes cholesterol excretion and has a suppressive effect on fatty liver. © Copyright 2016, Japanese Society for Food Science and Technology.


Masuda Y.,Kyushu University | Ono H.,Tokai Pickling Co. | Kitagawa H.,Tokai Pickling Co. | Ito H.,Tokai Pickling Co. | And 4 more authors.
Applied and Environmental Microbiology | Year: 2011

The culture supernatant of Leuconostoc mesenteroides TK41401, isolated from Japanese pickles, possessed antimicrobial activity against broad range of a bacterial genera and particularly strong activity against Bacillus coagulans, the major contaminant of pickles. An antimicrobial peptide was purified in three chromatographic steps, and its molecular mass was determined to be 6,115.59 Da by electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS). The primary structure of this peptide was determined by amino acid and DNA sequencing, and these analyses revealed that it was translated as a 63-residue precursor. This precursor showed high similarity to the precursor of lactocyclicin Q, a cyclic bacteriocin produced by Lactococcus sp. strain QU 12. The molecular weight calculated after cyclization, which was presumed to involve the same process as in lactocyclicin Q (between L3 and W63), agreed with that estimated by ESI-TOF MS. This peptide was proved to be a novel cyclic bacteriocin, and it was termed leucocyclicin Q. The antimicrobial spectrum of this bacteriocin clearly differed from that of lactocyclicin Q, even though their primary structures were quite similar. This is the first report of a cyclic bacteriocin produced by a strain of the genus Leuconostoc. © 2011, American Society for Microbiology.


Trademark
Tokai Pickling Co. | Date: 2012-01-31

pickled radish.


pickled radish.


Trademark
Tokai Pickling Co. | Date: 2012-01-31

Pickled radish.


Trademark
Tokai Pickling Co. | Date: 2012-01-31

Pickled radish.

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