Kakamigahara, Japan
Kakamigahara, Japan

Tokai Gakuin University is a private university in Kakamigahara, Gifu Prefecture, Japan. The predecessor of the school, founded in 1961, was chartered as Tokai Women's College in 1981. In 2007, the school adopted the present name. Wikipedia.


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Kimura K.,Kwansei Gakuin University | Izawa S.,Japanese National Institute for Occupational Safety and Health | Sugaya N.,Tokyo Metropolitan Institute of Medical Science | Ogawa N.,Waseda University | And 6 more authors.
Psychoneuroendocrinology | Year: 2013

Organisms prefer to receive rewards sooner rather than later because they excessively discount the subjective value of future rewards, a phenomenon called delay discounting. Recent studies have reported an association between cortisol-which is secreted by the hypothalamic-pituitary-adrenal (HPA) axis-and delay discounting. However, no study has examined whether acutely induced psychosocial stress modulates delay discounting. Thus, the present study examined the effect of acute psychosocial stress and its hormonal and inflammatory correlates on the rate of delay discounting. To accomplish this purpose, we assessed the participants' discounting rates using the questionnaire version with inter-temporal choice before and after an acute psychosocial stress task (the Trier Social Stress Test; TSST). The results demonstrated that TSST increased rates of delay discounting in only cortisol responders (not in non-responders), indicating the possible influence of the pathway from the HPA axis to the dopaminergic systems under acute stress. Furthermore, the findings of correlation analysis indicated a U-shaped relationship between baseline level of C-reactive protein and delay discounting rate, suggesting a complex relationship between inflammatory markers and delay discounting rate. © 2013 Elsevier Ltd.


Nakagawa T.,Gifu University | Mitsui R.,Okayama University of Science | Tani A.,Okayama University | Sasa K.,Gifu University | And 4 more authors.
PLoS ONE | Year: 2012

In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1. © 2012 Nakagawa et al.


Inada N.,National Institute of Mental Health | Koyama T.,National Institute of Mental Health | Inokuchi E.,National Institute of Mental Health | Kuroda M.,National Institute of Mental Health | And 2 more authors.
Research in Autism Spectrum Disorders | Year: 2011

Early detection and intervention is essential for children with autism spectrum disorders (ASD). Therefore, we examined the reliability and validity of the Japanese version of the Modified Checklist for autism in toddlers (M-CHAT), a 23-item, yes-no questionnaire regarding early autistic symptoms completed by parents of children at 18-24 months of age. Herein, the reliability of the M-CHAT was investigated for children 4-20 months of age. The M-CHAT score (the number of failed items) was found to be significantly correlated among 24 mother-father pairs (Pearson's r = .933), representing good inter-rater reliability. The test-retest reliability was satisfactory, with 22 mothers providing almost equal M-CHAT scores on two different occasions (r = .990). Significant correlations were observed between the M-CHAT score and the Childhood Autism Rating Scale-Tokyo version score in 25 two-year-old children (r = .581), indicating good concurrent validity. The M-CHAT score was significantly higher in 20 children later diagnosed with ASD compared with reference children (n = 1167), revealing sufficient discriminant validity. A short version of the M-CHAT using 9 items was proposed and effectively differentiated children with ASD from reference children. The efficacy of the Japanese version of the M-CHAT was demonstrated for first-level screening in the general population. © 2011 Elsevier Ltd.


Arakaki N.,Okinawa Prefectural Agricultural Research Center | Yamazawa H.,Japan National Institute of Agrobiological Science | Yamazawa H.,Tokai Gakuin University | Wakamura S.,Japan National Institute of Agrobiological Science | Wakamura S.,Kyoto Gakuen University
Applied Entomology and Zoology | Year: 2011

Egg parasitoids Telenomus euproctidis Wilcox (Hymenoptera: Scelionidae) were attracted to egg masses laid by wingless immobile female Orgyia postica (Lepidoptera: Lymantriidae). Virgin females, a solvent extract of pheromone glands, and a synthetic sex pheromone, (6Z,9Z,11S,12S)-11,12-epoxyhenicosa-6,9-diene (posticlure), also attracted this parasitoid in the field, demonstrating that T. euproctidis uses the sex pheromone of female Org. postica as a kairomone to locate host eggs. © 2011 The Japanese Society of Applied Entomology and Zoology.


Kato T.,Gifu University | Fujita Y.,Tokyo Metropolitan University | Nakane K.,Kakegawa Municipal General Hospital | Mizutani K.,Gifu University | And 7 more authors.
Cytokine | Year: 2013

Castration-refractory prostate cancer (CRPC) is treated with taxane-based chemotherapy, but eventually becomes drug resistant. It is thus essential to identify novel therapeutic targets for taxane resistance in CRPC patients. We investigated the role of the chemokine (C-C motif) receptor 1 (CCR1) and its ligand, chemokine (C-C motif) ligand 5 (CCL5), in taxane-resistant CRPC using paclitaxel-resistant prostate cancer cells (PC3PR) established from PC3 cells. We found that the expression levels of CCR1 mRNA and protein were up-regulated in PC3PR cells compared to PC3 cells. In order to investigate the role of increased CCR1 in PC3PR cells, we stimulated cells with CCL5, one of the chemokine ligands of CCR1. In CCL5-stimulated PC3PR cells, siRNA-mediated knockdown of CCR1 expression reduced phosphorylation of ERK1/2 and Rac1/cdc42. Furthermore, CCR1 knockdown and MEK1/2 inhibition decreased CCL5-stimulated secretion of MMPs 2 and 9, which play important roles in cancer cell invasion and metastasis. In the Matrigel invasion assay, knockdown of CCR1 and inhibition of the ERK and Rac signaling pathways significantly decreased the number of invading cells. Finally, the serum CCL5 protein level as measured by ELISA was not different among the three groups of patients: those with negative prostate biopsy, those at initial diagnosis of prostate cancer, and those with taxane-resistant prostate cancer. These results demonstrated for the first time that the interaction of CCR1 with CCL5 caused by increased expression of CCR1 promotes invasion of PC3PR cells by increasing secretion of MMPs 2 and 9 and by activating ERK and Rac signaling. Our findings suggest that CCR1 could be a novel therapeutic target for taxane-resistant CRPC. © 2013 Elsevier Ltd.


Kojima K.,Gifu University | Kojima K.,Gifu International Institute of Biotechnology | Fujita Y.,Gifu International Institute of Biotechnology | Nozawa Y.,Gifu International Institute of Biotechnology | And 3 more authors.
Prostate | Year: 2010

BACKGROUND Patients with hormone-refractory prostate cancer are treated with taxane drugs, but eventually become drug resistant. We aimed to elucidate the molecular mechanisms underlying paclitaxel resistance of hormone-refractory prostate cancer with a special focus on the roles of miR-34a and SIRT1. METHODS Paclitaxel-resistant cells (PC3PR) were generated from hormone-refractory PC3 cells. The expression levels of mRNA and miRNA were determined by reverse transcriptase PCR and those of protein were by Western blot analysis. Transfection of miRNA precursor or siRNA was performed using the liposome-mediated method. RESULTS MiR-34a over-expression and SIRT1 knockdown attenuated paclitaxel resistance of PC3PR cells. MiR-34a expression was reduced in PC3PR cells compared with PC3 cells, while the expression levels of HuR and Bcl2 as well as SIRT1 were elevated in PC3PR cells. Luciferase reporter assays revealed that both SIRT1 3′-UTR and promoter activities were higher in PC3PR cells than in PC3 cells. Introduction of miR-34a precursor into PC3PR cells resulted in decreases in HuR, Bcl2, and SIRT1 expression and inhibition of the SIRT1 3′-UTR activity. HuR knockdown reduced SIRT1 and Bcl2 expression. These results suggest that miR-34a not only directly but also indirectly via regulating HuR expression acts on the 3′-UTR of SIRT1 and Bcl2 mRNAs, thereby controlling their expression. Thus, in PC3PR cells, reduced expression of miR-34a confers paclitaxel resistance via up-regulating SIRT1 and Bcl2 expression. CONCLUSIONS MiR-34a and its downstream targets SIRT1 and Bcl2 play important roles in the development of paclitaxel resistance, all of which can be useful biomarkers and promising therapeutic targets for the drug resistance in hormone-refractory prostate cancer. © 2010 Wiley-Liss, Inc.


Fujita Y.,Gifu International Institute of Biotechnology | Kojima K.,Gifu International Institute of Biotechnology | Kojima K.,Gifu University | Ohhashi R.,Gifu International Institute of Biotechnology | And 9 more authors.
Journal of Biological Chemistry | Year: 2010

MicroRNAs are involved in cancer pathogenesis and act as tumor suppressors or oncogenes. It has been recently reported that miR-148a expression is down-regulated in several types of cancer. The functional roles and target genes of miR-148a in prostate cancer, however, remain unknown. In this report, we showed that miR-148a expression levels were lower in PC3 and DU145 hormone-refractory prostate cancer cells in comparison to PrEC normal human prostate epithelial cells and LNCaP hormone-sensitive prostate cancer cells. Transfection with miR-148a precursor inhibited cell growth, and cell migration and invasion, and increased the sensitivity to anti-cancer drug paclitaxel in PC3 cells. Computer-aided algorithms predicted mitogen- and stress-activated protein kinase, MSK1, as a potential target of miR-148a. Indeed, miR-148a overexpression decreased expression ofMSK1.Using luciferase reporter assays,weidentified MSK1 as a direct target of miR-148a. Suppression of MSK1 expression by siRNA, however, showed little or no effects on malignant phenotypes of PC3 cells. In PC3PR cells, a paclitaxel-resistant cell line established from PC3 cells, miR-148a inhibited cell growth, and cell migration and invasion, and also attenuated the resistance to paclitaxel. MiR-148a reduced MSK1 expression by directly targeting its 3′-UTR in PC3PR cells. Furthermore, MSK1 knockdown reduced paclitaxel-resistance of PC3PR cells, indicating that miR-148a attenuates paclitaxel-resistance of hormone-refractory, drug-resistant PC3PR cells in part by regulating MSK1 expression. Our findings suggest that miR-148a plays multiple roles as a tumor suppressor and can be a promising therapeutic target for hormone-refractory prostate cancer especially for drug-resistant prostate cancer. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.


Izawa S.,Japanese National Institute for Occupational Safety and Health | Izawa S.,Waseda University | Saito K.,Tokai University | Shirotsuki K.,Tokai Gakuin University | And 2 more authors.
Psychoneuroendocrinology | Year: 2012

This study investigated variations in salivary levels of cortisol and dehydroepiandrosterone (DHEA) in a prolonged stressful situation (a two-week teaching practice). Thirty-three women for whom a two-week teaching practice at a kindergarten was scheduled were asked to collect saliva samples at awakening, 30. min after awakening, and bedtime at four time points: two weeks before the practice, the first week of the practice, the second week of the practice, and a few days after the practice. In addition, they completed questionnaires for assessing perceived stress and subjective moods on each day. A linear mixed model indicated that cortisol levels significantly increased during the first and second week of the practice compared with those before and after the practice period, and that DHEA levels significantly decreased after the practice period compared with those at the other time points. Further, cortisol awakening response after the practice period significantly reduced compared with that at the other time points. Scores of perceived stress and negative moods were also higher during the practice period. This study showed that prolonged stress affected cortisol and DHEA secretion during as well as after the stress period. © 2011 Elsevier Ltd.


Hasegawa A.,Tokai Gakuin University | Hattori Y.,Kyoto Gakuen University | Nishimura H.,University of Tsukuba | Tanno Y.,University of Tokyo
Psychological Reports | Year: 2015

The main purpose of this study was to examine whether depressive rumination and social problem solving are prospectively associated with depressive symptoms. Nonclinical university students (N = 161, 64 men, 97 women; M age = 19.7 yr., SD = 3.6, range = 18–61) recruited from three universities in Japan completed the Beck Depression Inventory–Second Edition (BDI–II), the Ruminative Responses Scale, Social Problem-Solving Inventory–Revised Short Version (SPSI– R:S), and the Means–Ends Problem-Solving Procedure at baseline, and the BDI–II again at 6 mo. later. A stepwise multiple regression analysis with the BDI–II and all subscales of the rumination and social problem solving measures as independent variables indicated that only the BDI–II scores and the Impulsivity/carelessness style subscale of the SPSI–R:S at Time 1 were signifi cantly associated with BDI–II scores at Time 2 (β = 0.73, 0.12, respectively; independent variables accounted for 58.8% of the variance). These fi ndings suggest that in Japan an impulsive and careless problem-solving style was prospectively associated with depressive symptomatology 6 mo. later, as contrasted with previous fi ndings of a cycle of rumination and avoidance problem-solving style. © Psychological Reports 2015.


Iio A.,Gifu International Institute of Biotechnology | Ohguchi K.,Gifu International Institute of Biotechnology | Ohguchi K.,Sugiyama Jogakuen University | Iinuma M.,Gifu Pharmaceutical University | And 3 more authors.
Journal of Natural Products | Year: 2012

ABCA1, a member of the ATP-binding cassette transporter family, regulates high-density lipoprotein (HDL) metabolism and cholesterol transport. Its expression is upregulated mainly by the activation of the liver X receptor (LXR). Since ABCA1 plays a pivotal role in cholesterol and HDL metabolism, identification of a compound capable of increasing its expression may be beneficial for the prevention and therapy of atherosclerosis. Firefly luciferase reporter assays were developed for human ABCA1 promoters and LXR enhancers, and an in-house phytochemical library was screened. It was found that a citrus flavonoid, hesperetin (1), increased ABCA1 promoter and LXR enhancer activities in THP-1 macrophages. It was also found that this flavonoid promoted PPAR-enhancing activity. In accordance with these findings, 1 increased mRNA and protein expression of ABCA1 and consequently upregulated ApoA-I-mediated cholesterol efflux. These results provide evidence that 1 promotes ApoA-I-mediated cholesterol efflux from macrophages by increasing ABCA1 expression through the activation of LXRα and PPARγ. © 2012 The American Chemical Society and American Society of Pharmacognosy.

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