Tochigi Prefectural Museum

Utsunomiya-shi, Japan

Tochigi Prefectural Museum

Utsunomiya-shi, Japan
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Ishii C.,Hokkaido University | Nakayama S.M.M.,Hokkaido University | Ikenaka Y.,Hokkaido University | Ikenaka Y.,North West University South Africa | And 8 more authors.
Chemosphere | Year: 2017

Lead (Pb) poisoning is widespread among raptors and water birds. In Japan, fragments of Pb ammunition are still found in endangered eagles although more than 10 years have passed since legislation regarding use of Pb ammunition was introduced. This study was performed to investigate Pb exposure in raptors from various locations in Japan. We measured hepatic and renal Pb concentrations and hepatic Pb isotope ratios of Steller's sea eagles (Haliaeetus pelagicus), white-tailed sea eagles (Haliaeetus albicilla), golden eagles (Aquila chrysaetos), and 13 other species (total 177 individuals) that were found dead, as well as blood samples from three eagles found in a weakened state during 1993–2015 from Hokkaido (northern part), Honshu (the main island), and Shikoku (a southern island) of Japan. In the present study in Hokkaido, one quarter of the sea eagles showed a high Pb concentration, suggesting exposure to abnormally high Pb levels and Pb poisoning. Pb isotope ratios indicated that endangered Steller's sea eagle and white-tailed sea eagle were poisoned by Pb ammunition that was used illegally in Hokkaido. In other areas of Japan, both surveillance and regulations were less extensive than in Hokkaido, but Pb poisoning in raptors was also noted. Therefore, Pb poisoning is still a serious problem in raptors in various areas of Japan due to accidental ingestion of materials containing Pb, especially Pb ammunition. © 2017


Ando Y.,Mizunami Fossil Museum | Kawano S.,Tochigi Prefectural Museum | Ugai H.,Goshoura Cretaceous Museum
Neues Jahrbuch fur Geologie und Palaontologie - Abhandlungen | Year: 2015

Stomatopod mandibles and raptorial claws and fourteen species of decapods including Paradorippe sp. cf. P. granulata (de Haan, 1841), Hiplyra platycheir (de Haan, 1841), Myra sp. cf. M. celeries Galil, 2001, Halimede fragifer (de Haan, 1835), Eucrate crenata (de Haan, 1835) and Entricoplax vestita (de Haan, 1833) are described from the upper Pleistocene Ogushi Formation in Amakusa City, Kumamoto Prefecture, Kyushu, Japan. Alpheidae gen. et sp. indet. is abundant and Eucrate crenata and Entricoplax vestita are common in the decapod fauna of the Ogushi Formation. The occurrence of these species suggests that the Ogushi Formation was deposited as muddy bottom sediment in shallow marine water. Euplax leptophthalmus H. Milne edwards, 1852, a rare macrophthalmine crab is first reported as a fossil from Kyushu. © 2015 E. Schweizerbart'sche Verlagsbuchhandlung, Stuttgart, Germany.


Mizukawa H.,Ehime University | Nomiyama K.,Ehime University | Nakatsu S.,Nakatsu Veterinary Surgery | Yachimori S.,Shikoku Institute of Natural History | And 4 more authors.
Environmental Pollution | Year: 2013

Residue levels and patterns of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), their hydroxylated metabolites (OH-PCBs, OH-PBDEs), and methoxylated PBDEs (MeO-PBDEs) in the blood of various terrestrial mammals in Japan, including cats, raccoon dogs, dogs, masked palm civets, foxes, raccoons, badgers, and mongooses were determined. Tri- through penta-chlorinated OH-PCBs were predominant in cat blood, whereas hexa- through octa-chlorinated OH-PCBs were found in other species. High proportion of BDE209 was found in all species, suggesting exposure to municipal waste and soil containing higher levels of deca-BDE products. 6OH-/MeO-BDE47 and 2′OH-/MeO-BDE68 were dominant in all terrestrial mammals. This is first report on the detection of OH-/MeO-PBDEs in the blood of terrestrial mammals. High concentrations of OH-/MeO-PBDEs were found in cats, suggesting the intake of these compounds from seafood. Cats exhibited higher accumulation and specific patterns of OH-PCBs, OH-PBDEs, and MeO-PBDEs, they may be at a high risk from these metabolites. © 2012 Elsevier B.V. All rights reserved.


Tominaga A.,University of Ryukyus | Matsui M.,Kyoto University | Yoshikawa N.,Kyoto University | Nishikawa K.,Kyoto University | And 4 more authors.
Molecular Phylogenetics and Evolution | Year: 2013

We investigated the phylogenetic relationships and estimated the historical demography of the Japanese fire-bellied newt, Cynops pyrrhogaster, from Japanese mainlands using 1407-bp sequences of the mitochondrial DNA (NADH6, tRNAglu, cyt b) and 1208-bp sequences of nuclear DNA (Rag-1) genes. Phylogenetic trees based on mitochondrial DNA revealed four major haplotype clades (NORTHERN, CENTRAL, WESTERN, and SOUTHERN clades) within this species. Degree of genetic differentiation among major haplotype clades was very large for intraspecific variation, suggesting this species to be composed of four species lineages that replace each other geographically. Nuclear genetic variation presented no obvious patterns of geographic structure except for the distinctness of populations diagnosed by NORTHERN clade of mitochondrial haplotypes, suggesting results of incomplete lineage sorting. Current distribution and estimated divergence times for the genus Cynops suggest that the common ancestor of two Japanese species (C. pyrrhogaster and C. ensicauda from the Ryukyu Islands) had diverged at the edge of the continent corresponding to the present East China Sea and Central Ryukyus. Subsequent range expansion to Japanese mainland seems to have occurred in the middle Miocene. Population-genetic analyses indicated that all species lineages, except for the SOUTHERN one, experienced geographic population reductions and expansions associated with glacial and postglacial climatic oscillations. © 2012 Elsevier Inc.


PubMed | Ehime University, Tochigi Prefectural Museum, Matsuyama University and Prefectural University of Kumamoto
Type: | Journal: Journal of chromatography. A | Year: 2014

In the present study, a sensitive and accurate isotope dilution method was developed for the simultaneous determination of 17 polar pharmaceutical and personal care product (PPCP) residues (logKow=1.40-5.74), including 14 pharmaceuticals and 3 personal care products, in biological organs and tissues. The proposed method involved enzymatic hydrolysis, followed by sequential clean-up using silica gel chromatography and gel permeation chromatography, and analysis via ultra-high-performance liquid chromatography with tandem mass spectrometry. This method yielded acceptable absolute recoveries (48-88%) and internal standard-corrected recoveries (90-130%) for 17 PPCPs. Method detection limits were between 0.0092 and 3.2ngg(-1) wet weight, and the limits of quantification were between 0.020 and 8.7ngg(-1) wet weight. The method can be used to readily detect the target compounds at trace levels while minimizing the required sample volume. The developed method was applied to the determination of 17 PPCPs in the liver and kidney of 17 birds collected from Japan and also in the plasma, liver, and brain of 7 cyprinoid fish from an effluent-dominated stream in Japan. Triclosan was detected in 5 of 11 fish-eating birds but not in non-fish-eating birds, suggesting the contamination of prey fish by the chemical. Non-steroidal anti-inflammatory drugs, antibacterial agents, and psychotropic agents were frequently detected in the fish tissues. In addition, 7 of the target compounds were found in fish brain. The median brain/plasma ratios of the psychotropic agents ranged from 1.6 (carbamazepine) to 12 (diphenhydramine), indicating high transportability to fish brain.


Nishioka Y.,Waseda University | Hirayama R.,Waseda University | Kawano S.,Tochigi Prefectural Museum | Ogino S.,Tamba Dinosaurs Fossil Studio
Paleontological Research | Year: 2016

Dental fossils of an indeterminate small cervoid (Ruminantia, Artiodactyla, Mammalia) from the lower Miocene Koura Formation of southwestern Japan are described. The examined specimens of Cervoidea fam., gen. et sp. indet. consist of selenodont lower molars with plesiomorphic characteristics of early cervoids: a strong external postprotocristid (or Palaeomeryx-fold), a deep groove on the lingual wall of the internal postmetacristid, and opening posterior cristids between the postentocristid and the posthypocristid. The lower molars are similar in shape and size with those of Miocene Cervidae rather than Moschidae or Palaeomerycidae because the present specimens are comparable to those of Dicrocerus tokunagai, which is one of the lower/ middle Miocene cervids from northeastern Japan, and D. grangeri from the middle Miocene of China. The lower Miocene Nakamura Formation (ca. 19 Ma) of central Japan also yielded a small cervoid species sharing the lower molar characteristics with Cervoidea fam., gen. et sp. indet. from the Koura Formation, suggesting a faunal or biostratigraphical correlation between the two formations. © by the Palaeontological Society of Japan.


Ando Y.,Mizunami Fossil Museum | Kishimoto S.,3 65 3 Komatsu cho | Kawano S.,Tochigi Prefectural Museum
Neues Jahrbuch fur Geologie und Palaontologie - Abhandlungen | Year: 2016

Thalassina tsuyamensis ANDO & KISHIMOTO n. sp. is described from the lower to middle Miocene Yoshino Formation of the Katsuta Group in the Okayama Prefecture, Japan. This species is similar to the extant T. saetichelis SAKAI & TÜRKAY, 2012, but differs in having a lateral subdorsal carina on the dorsal border of the propodus with tubercles extending posterior to the anterior part of pereiopod 1. Thalassina yamato ANDO & KISHIMOTO n. sp. is described from the middle Miocene Kawachi Formation of the Kukinaga Group in the Kagoshima Prefecture, Japan. This new species is closest to the extant T. squamifera DE MAN, 1915. However, it differs from T. squamifera in having a lateral dorsal carina on the dorsal border of the propodus of pereiopod 1 extending to only one- Third length and an unarmed dorsal border of the merus of pereiopods 3 to 5. © 2016 E. Schweizerbart'sche Verlagsbuchhandlung, Stuttgart, Germany.


Irizuki T.,The University of Shimane | Ito H.,The University of Shimane | Sako M.,The University of Shimane | Yoshioka K.,The University of Shimane | And 3 more authors.
Marine Pollution Bulletin | Year: 2015

Two sediment cores were obtained from Kasado Bay, a moderate-polluted enclosed bay in Japan, to examine anthropogenic impacts on Ostracoda over the past ca. 70years. We analyzed ostracode abundance and diversity, grain size, and CHN, and used 210Pb and 137Cs as the dating method. The present study showed that cross-plot comparisons of ostracode abundance and each environmental factor, based on sediment core data, could be used to identify ostracode species as indicators for anthropogenic influences. Ostracode abundance reflected mainly the changes that had occurred in total organic carbon content in sediments related to eutrophication, but heavy metal concentration did not directly influence several ostracode abundance in the bay. Environmental deterioration because of eutrophication started in the 1960s. The regulations regarding the chemical oxygen demand in waters introduced in the 1980s probably influence ostracode abundance for certain species in this period. Currently, Kasado Bay is not experiencing severe degradation. © 2014 Elsevier Ltd.


Tanoue R.,Ehime University | Nomiyama K.,Ehime University | Nakamura H.,Prefectural University of Kumamoto | Hayashi T.,Tochigi Prefectural Museum | And 4 more authors.
Journal of Chromatography A | Year: 2014

In the present study, a sensitive and accurate isotope dilution method was developed for the simultaneous determination of 17 polar pharmaceutical and personal care product (PPCP) residues (logKow=1.40-5.74), including 14 pharmaceuticals and 3 personal care products, in biological organs and tissues. The proposed method involved enzymatic hydrolysis, followed by sequential clean-up using silica gel chromatography and gel permeation chromatography, and analysis via ultra-high-performance liquid chromatography with tandem mass spectrometry. This method yielded acceptable absolute recoveries (48-88%) and internal standard-corrected recoveries (90-130%) for 17 PPCPs. Method detection limits were between 0.0092 and 3.2ngg-1 wet weight, and the limits of quantification were between 0.020 and 8.7ngg-1 wet weight. The method can be used to readily detect the target compounds at trace levels while minimizing the required sample volume. The developed method was applied to the determination of 17 PPCPs in the liver and kidney of 17 birds collected from Japan and also in the plasma, liver, and brain of 7 cyprinoid fish from an effluent-dominated stream in Japan. Triclosan was detected in 5 of 11 fish-eating birds but not in non-fish-eating birds, suggesting the contamination of prey fish by the chemical. Non-steroidal anti-inflammatory drugs, antibacterial agents, and psychotropic agents were frequently detected in the fish tissues. In addition, 7 of the target compounds were found in fish brain. The median brain/plasma ratios of the psychotropic agents ranged from 1.6 (carbamazepine) to 12 (diphenhydramine), indicating high transportability to fish brain. © 2014 Elsevier B.V.

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