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Yao D.,Huazhong Agricultural University | Ji Z.,Huazhong Agricultural University | Wang C.,Tobacco Research Institute of Hubei Province | Qi G.,Huazhong Agricultural University | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012

The strain 3-10 was isolated from soil and identified as B. subtilis according to morphological and physiological characteristics and nucleotide sequence of 16S rRNA. It co-produced anti-fungal iturin A and fertilizer synergist of poly-γ-glutamic acid (γ-PGA) under solid state fermentation (SSF) with rapeseed meal. The co-production of iturin A and γ-PGA reached 5. 3 and 51. 3 g/kg-dry weight culture, respectively, and the number of viable cells reached 1. 9 × 1010 CFU/g-dry weight culture. In pot tests, the shoot length and dry weight of watermelon seedlings treated by the SSF culture improved by 48. 0 and 30. 8%, respectively compared to the control; and its biocontrol effect on watermelon fusarium wilt achieved 89. 6%. These results highlight a novel strategy to exploit the low-cost and widely available rapeseed meal as dual-functional bio-organic fertilizer under SSF by B. subtilis. © 2011 Springer Science+Business Media B.V.


Chen Z.,Huazhong University of Science and Technology | Chen Z.,Tobacco Research Institute of Hubei Province | Wu J.,Huazhong University of Science and Technology | Qiao Y.,Huazhong University of Science and Technology | Xu M.,Huazhong University of Science and Technology
Huazhong Keji Daxue Xuebao (Ziran Kexue Ban)/Journal of Huazhong University of Science and Technology (Natural Science Edition) | Year: 2013

Leaching characteristics of trace element Se of an Enshi stone coal was investigated. The effects of pH value of leaching solution, ratio of liquid to solid and leaching time on the Se concentration in the leachate were not significant. The most content of Se was residual in coal particles. The combustion experiments of the stone coal and a Shenhua bituminous coal were also carried out in a drop-tube furnace in air conditions. The burnout of the Enshi stone coal increased from 90.0% to 94.6% with the temperature increasing from 1000 to 1300°C. It is clearly indicated that the reactivity of the stone coal is much lower than that of the bituminous coal. Computational fluid dynamics (CFD) modeling shows that the blend of stone coal and bituminous coal is feasible in a 600 MW boiler. However, the blending ratio could not be too high due to the possibility of flameout in boiler and the slagging/fouling in the super-heater, re-heater and economizer of the furnace, which will affect the safety and economy of boiler.


Xiong H.,Hubei University | Zhao Y.,Tobacco Research Institute of Hubei Province | Liu P.,Hubei University | Zhang X.,Hubei University | Wang S.,Hubei University
Microchimica Acta | Year: 2010

Electrochemical properties of nicotine at the glassy carbon electrode modified with multi-walled carbon nanotubes were explored. Nicotine underwent irreversible reduction at the modified electrode, which was an adsorption-controlled process with two protons and two electrons. The reductive peak current of nicotine significantly increased at the modified electrode compared with the bare glassy carbon electrode, suggesting that the multi-walled carbon nanotubes can enhance the electron transfer rate. The current was proportional to the concentration of nicotine over two line ranges, and the detection limit was 9.3 μM (at S/N=3). For ten parallel detections of 0.62 mM nicotine, the relative standard deviation was 2.67%, suggesting that the film modified electrode had excellent reproducibility. The modified electrode was applied to the direct determination of nicotine in tobacco samples with good sensitivity, selectivity and stability. © Springer-Verlag 2009.


Wang J.,Chinese Academy of Agricultural Sciences | Li Y.,Tobacco Research Institute of Hubei Province | Shen L.,Chinese Academy of Agricultural Sciences | Qian Y.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Pesticide Biochemistry and Physiology | Year: 2015

The inhibitory effects of sulfated lentinan with different degrees of sulfation against tobacco mosaic virus (TMV) and the underlying mechanism were investigated. The results indicated that plants treated with increasing concentrations of sulfated lentinan, with increasing numbers of treatments and with increasing time after treatment had a decrease in the number of necrotic lesions, indicating a long-term protection against TMV that mimics vaccination. In addition, the levels of TMV-capsid protein (CP) transcripts decreased in distant leaves, indicating that sulfated lentinan induces systemic protection against TMV. The activities of the defense enzymes phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX) and the amounts of several phenylpropanoid compounds (PPCs) were measured in control and treated plants without infection. A progressive increase in PAL activity was observed with increasing time after treatment, together with the accumulation of free and conjugated PPCs. In contrast, LOX activity remained unchanged. Interestingly, the increase in PAL activity showed a linear correlation with the decrease in necrotic lesions and the decrease in TMV-CP transcript level. Thus, sulfated lentinan induced systemic and long-term protection against TMV in tobacco plants that is determined, at least in part, by a sustained activation of PAL and the accumulation of PPCs with potential antiviral activity. © 2014 Elsevier Inc.


Patent
Tobacco Research Institute Of Hubei Province | Date: 2010-05-11

One aspect of the invention provides an agent for reducing nicotine and harmful components in tobacco leaves for use in the field cultivation of tobacco. In one embodiment, the agent contains (in a weight percentage): salicylic acid 0.005-0.16%, naphthaleneacetic acid 0-0.003%, indoleacetic acid 0-0.015%, and clean water added to 100%. The composition can significantly reduce nicotine and harmful components in tobacco leaves by applying it to tobacco plant in top pruning period of tobacco. The combination of salicylic acid, naphthaleneacetic acid and indoleacetic acid provides synergic effect for reducing nicotine. The nicotine-reducing agent also can result in a yield increase and improve the aroma of tobacco.

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