Tobacco Research Institute of Hubei Province

Wuhan, China

Tobacco Research Institute of Hubei Province

Wuhan, China
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Cao J.,Tobacco Research Institute of Hubei Province | Yu J.,Tobacco Research Institute of Hubei Province
Tobacco Science and Technology | Year: 2017

To gain knowledge on the utilization of flue-cured tobacco hybrids in Zimbabwe and to take it as a reference for improving the ability of flue-cured tobacco breeding in China, the concept and strategy of flue-cured tobacco breeding, leaf research work and general program of hybrid utilization, the regional test, popularization and application of hybrids in Zimbabwe were introduced and analyzed on the basis of on-site investigation and research. In Zimbabwe, the flue-cured tobacco varieties bred in recent 20 years were all male sterile hybrids. Hybrid varieties had the advantage in speeding up breeding of new multi disease-resistant varieties. However, multi-resistant parents were the basis for the aggregation of resistances to more diseases in hybrids. Therefore, the breeding of superior parents with multi-resistant traits was the key of hybrid utilization, and effective resistant resources to most of major diseases in Zimbabwe tobacco production were screened out from germplasm resources. The resistance genes were successfully transferred into flue-cured tobacco cultivars, and a number of quality flue-cured tobacco parent materials with resistances to several diseases were bred, which built a foundation for the breeding of multi-resistant hybrids. Comparing with Zimbabwe practice, the number of quality multi-resistant hybrids independently bred and popularized in flue-cured tobacco production was less in China; the fundamental cause was the serious lack of quality parent materials with resistances to several diseases. Therefore, the research and utilization of disease-resistant germplasm in flue-cured tobacco breeding should be strengthened in China. At the same time, the breeding of quality multi-resistant parent materials should also be valued for making full use of hybrids. © 2017, Editorial Office of Tobacco Science and Technology. All right reserved.


Sun G.,Tobacco Research Institute of Hubei Province | Sun J.,Tobacco Research Institute of Hubei Province | Wang K.,Hubei Provincial Tobacco Quality Supervision and Test Station | Li J.,Tobacco Research Institute of Hubei Province | And 2 more authors.
Tobacco Science and Technology | Year: 2016

To study the effects of air-curing time on aroma components, TSNA contents and smoking quality of flue-cured tobacco, flue-curing experiments were conducted in an electrothermal barn. Tobacco leaves were flue-cured after harvesting directly or after air-curing for 24, 48, 72, 96 hours, respectively. The contents of neutral aroma components and TSNAs in flue-cured tobacco leaves were determined, and the leaf sensory quality was evaluated. The results showed that with the prolonged air-curing before flue-curing, the contents of neutral aroma compounds increased, while sensory quality increased first then decreased. TSNA contents slowly increased at the early stage of air-curing, then the rate of increase speeded up significantly after 72 hours. Therefore, controlling the air-curing time before flue-curing within 48-72 hours is beneficial to improving the aroma component content and smoking quality and reducing the TSNAs content of flue-cured tobacco. © 2016, Editorial Office of Tobacco Science & Technology. All right reserved.


PubMed | Tobacco Research Institute of Hubei Province, Chinese Academy of Agricultural Sciences and Baoshan Oriental Tobacco Co.
Type: | Journal: Pesticide biochemistry and physiology | Year: 2015

The inhibitory effects of sulfated lentinan with different degrees of sulfation against tobacco mosaic virus (TMV) and the underlying mechanism were investigated. The results indicated that plants treated with increasing concentrations of sulfated lentinan, with increasing numbers of treatments and with increasing time after treatment had a decrease in the number of necrotic lesions, indicating a long-term protection against TMV that mimics vaccination. In addition, the levels of TMV-capsid protein (CP) transcripts decreased in distant leaves, indicating that sulfated lentinan induces systemic protection against TMV. The activities of the defense enzymes phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX) and the amounts of several phenylpropanoid compounds (PPCs) were measured in control and treated plants without infection. A progressive increase in PAL activity was observed with increasing time after treatment, together with the accumulation of free and conjugated PPCs. In contrast, LOX activity remained unchanged. Interestingly, the increase in PAL activity showed a linear correlation with the decrease in necrotic lesions and the decrease in TMV-CP transcript level. Thus, sulfated lentinan induced systemic and long-term protection against TMV in tobacco plants that is determined, at least in part, by a sustained activation of PAL and the accumulation of PPCs with potential antiviral activity.


Cai C.,Tobacco Research Institute of Hubei Province | Yang Y.,Hubei University | Cheng L.,Tobacco Research Institute of Hubei Province | Tong C.,Chinese Academy of Agricultural Sciences | Feng J.,Tobacco Research Institute of Hubei Province
Russian Journal of Genetics | Year: 2015

Because of the advantages of EST-SSR markers, it has been employed as powerful markers for genetic diversity analysis, comparative mapping and phylogenetic studies. In this study, a total of 429,869 tobacco (Nicotiana tabacum L.) ESTs were downloaded from the public databases, which offers an opportunity to identify SSRs in ESTs by data mining, and 38,165 SSRs were identified from 379,967 uni-ESTs with the frequency of one SSR per 5.52 kb. Mono and tri-nucleotide repeat motifs were the dominant repeat types, accounting for 40.53 and 34.51% of all SSRs, respectively. After eliminating mononucleotide -containing sequences, 86 pairs of primers were designed to amplify in four tobacco accessions. Only 15 primers (17.44%) showed polymorphism, and then they were further used to assess genetic diversity of 20 tobacco accessions. Unweighted pair-group method with arithmetic average dendrograms (UPGMA) and principal coordinates analysis plots (PCA) revealed genetic differentiation between N. rustica and N. tabacum, and between oriental tobacco and other accessions of N. tabacum. The present study reported the development of EST-SSR markers in tobacco by exploiting EST databases, and confirmed the effective way to develop mark ers. These EST-SSRs can serve in studies on cultivar identification, genetic diversity analysis, and genetics in tobacco. © Pleiades Publishing, Inc., 2015.


Wei X.,Huazhong Agricultural University | Deng X.,Huazhong Agricultural University | Cai D.,Huazhong Agricultural University | Ji Z.,Huazhong Agricultural University | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Tobacco specific nitrosamines (TSNA) mainly consisting of N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are a group of toxic components threatening human health. To inhibit TSNA formation in tobacco leaves, a high nitrite reductive strain with low nitrate reduction ability was isolated and applied to tobacco leaves in an attempt to lower the nitrite precursor of TSNA. By morphology, physiology, biochemistry, and 16S rDNA sequence analysis, the strain DA9 was identified as Bacillus amyloliquefaciens. Under the optimized fermentation parameters (glucose 40 g/L, NH4Cl 4 g/L, corn steep liquor 8 g/L, MnSO4 0.01 g/L, KH2PO4 1.0 g/L, MgSO4 0.3 g/L, initial pH 7.0, inoculum age 6 h, inoculum size 3%, temperature 37 °C), the maximum cell dentisity of 1.2 × 109 CFU/mL was obtained at 36 h. The DA9 cell suspensions were applied in the air-curing process of the Burley tobacco (Eyan 6) leaves. The treatment by DA9 cells lowered 32% of the nitrite content and 47% of total TSNA content in the tobacco leaves, and the concentrations of the NNN, NNK, and NAT were decreased by 48%, 12%, and 35%, respectively. Collectively, this study provides a promising strain and a novel strategy for decreasing TSNA during the air-curing process. © 2014 American Chemical Society.


Xiong H.,Hubei University | Zhao Y.,Tobacco Research Institute of Hubei Province | Liu P.,Hubei University | Zhang X.,Hubei University | Wang S.,Hubei University
Microchimica Acta | Year: 2010

Electrochemical properties of nicotine at the glassy carbon electrode modified with multi-walled carbon nanotubes were explored. Nicotine underwent irreversible reduction at the modified electrode, which was an adsorption-controlled process with two protons and two electrons. The reductive peak current of nicotine significantly increased at the modified electrode compared with the bare glassy carbon electrode, suggesting that the multi-walled carbon nanotubes can enhance the electron transfer rate. The current was proportional to the concentration of nicotine over two line ranges, and the detection limit was 9.3 μM (at S/N=3). For ten parallel detections of 0.62 mM nicotine, the relative standard deviation was 2.67%, suggesting that the film modified electrode had excellent reproducibility. The modified electrode was applied to the direct determination of nicotine in tobacco samples with good sensitivity, selectivity and stability. © Springer-Verlag 2009.


Cai C.,Tobacco Research Institute of Hubei Province | Deng H.,Wuhan Regional Climate Center | Cheng L.,Tobacco Research Institute of Hubei Province | Wang P.,China Tobacco Hubei Industrial Co. | Feng J.,Tobacco Research Institute of Hubei Province
Tobacco Science and Technology | Year: 2015

In order to breed new flue-cured tobacco lines of harmonious chemical composition via scientific and rational parent selection and combination collocation, thirty complete diallel cross combinations were prepared from six flue-cured tobacco varieties with Griffing method (III). The genetic effects of eight chemical component indexes (the contents of nicotine, total nitrogen, total sugar, potassium, chlorine and the ratios of total nitrogen/nicotine, total sugar/ nicotine, potassium/chlorine) in tobacco leaves were preliminarily analyzed. The results showed that the said chemical indexes differed extremely significantly between combinations, the general combining ability (GCA) between parents and the specific combining ability (SCA) between combinations exhibited extremely significantly differences as well. Moreover, the variance of GCA was greater than that of SCA for all the indexes except for the ratio of total nitrogen/ nicotine. It indicated that for most of the chemical indexes investigated in this experiment, parent selection was more important than combination collocation. For GCA effect, cv. RG17 possessed a potential in increasing potassium content and cv. Nanjiang No.3 might increase potassium/chlorine ratio; 6388 lines presented a potential in decreasing chlorine content. Analyzing SCA effect from the point of chemical quality, the combination of K326 ×Nanjiang No.3, Jingyehuang × Nanjiang No.3 and K8 × Jingyehuang were the best, followed by K326 × Jingyehuang and K8×RG17. There existed obvious heritability differences between the traits of chemical indexes. Potassium content and total sugar content possessed the highest and the lowest special heritability of 33.82% and 1.37%, respectively. The general heritability of chlorine content (51.84% ) and potassium content (50.04%) was the highest, while that of total sugar content (15.08%) was the lowest. Only the special heritability of potassium content accounted for over 50% of general heritability, it indicated that the heritability of potassium content mainly was additive effect, which facilitated to the breeding tobacco cultivars of high-potassium content and was suitable to line selection at earlier generation. For the traits of other chemical indexes, it would be preferable to carry out line selection while the trait became stable. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Yao D.,Huazhong Agricultural University | Ji Z.,Huazhong Agricultural University | Wang C.,Tobacco Research Institute of Hubei Province | Qi G.,Huazhong Agricultural University | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012

The strain 3-10 was isolated from soil and identified as B. subtilis according to morphological and physiological characteristics and nucleotide sequence of 16S rRNA. It co-produced anti-fungal iturin A and fertilizer synergist of poly-γ-glutamic acid (γ-PGA) under solid state fermentation (SSF) with rapeseed meal. The co-production of iturin A and γ-PGA reached 5. 3 and 51. 3 g/kg-dry weight culture, respectively, and the number of viable cells reached 1. 9 × 1010 CFU/g-dry weight culture. In pot tests, the shoot length and dry weight of watermelon seedlings treated by the SSF culture improved by 48. 0 and 30. 8%, respectively compared to the control; and its biocontrol effect on watermelon fusarium wilt achieved 89. 6%. These results highlight a novel strategy to exploit the low-cost and widely available rapeseed meal as dual-functional bio-organic fertilizer under SSF by B. subtilis. © 2011 Springer Science+Business Media B.V.


Li Y.,Tobacco Research Institute of Hubei Province | Li Y.,Huazhong Agricultural University | Li X.,Tobacco Research Institute of Hubei Province | Han D.,Huazhong Agricultural University | And 3 more authors.
Tobacco Science and Technology | Year: 2014

In order to reveal the Se (IV) absorption and utilization rules of flue-cured tobacco, hydroponic experiments were carried out to study the kinetics characteristics of Se (IV) absorption of flue-cured tobacco cv. K326 and cv. Yunyan87, at different pH, accompanying ions and inhibitors by referring to the principles of nutrient absorption kinetics. The results indicated that: 1) At low Se (IV) level (100 g/L), the absorption and accumulation rate and affinity of K326 to Se (IV) was higher than those of Yunyan87. K326 was more adaptable to nutrient absorption at a low Se (IV) level. 2) Weak acidic or neutral condition (pH 5-7) facilitated to Se (IV) absorption and accumulation, while an alkaline condition did not. 3) Accompanying ions, such as SO4 2- and PO4 3-, inhibited the absorption and accumulation rate and decreased the affinity of flue-cured tobacco to Se (IV), especially PO4 3-. 4) Both uncoupling metabolic inhibitor (CCCP) and Ca2+ channel inhibitor (LaCl3) inhibited Se (IV) absorption of flue-cured tobacco, especially CCCP. ©, 2014, Editorial Office of Tobacco Science and Technology. All right reserved.


Patent
Tobacco Research Institute Of Hubei Province | Date: 2010-05-11

One aspect of the invention provides an agent for reducing nicotine and harmful components in tobacco leaves for use in the field cultivation of tobacco. In one embodiment, the agent contains (in a weight percentage): salicylic acid 0.005-0.16%, naphthaleneacetic acid 0-0.003%, indoleacetic acid 0-0.015%, and clean water added to 100%. The composition can significantly reduce nicotine and harmful components in tobacco leaves by applying it to tobacco plant in top pruning period of tobacco. The combination of salicylic acid, naphthaleneacetic acid and indoleacetic acid provides synergic effect for reducing nicotine. The nicotine-reducing agent also can result in a yield increase and improve the aroma of tobacco.

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