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Luo Z.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Wang C.,China Tobacco Zhejiang Industrial Co. | Chen Q.,China Tobacco Zhejiang Industrial Co. | And 3 more authors.
Tobacco Science and Technology | Year: 2015

In order to clarify the resistance of Hubei origin germplasms of air-cured and sun-cured tobaccos to powdery mildew, 88 germplasms of air-cured and sun-cured tobacco were evaluated in greenhouse by spraying inoculation with the fungal isolate kept by Tobacco Research Institute of Chinese Academy of Agricultural Sciences. The results showed that the resistance to powdery mildew differed significantly, 13 were highly resistant (HR), 39 moderately resistant (MR), 18 moderately susceptible (MS), and 18 highly susceptible (HS) among the total 88 germplasms. The highest resistant ones were LC04, ZX06 and WF04, with relative resistant indexes of 0.99. The highest susceptible ones were FJY01 and CY08, with relative resistant indexes of less than 0.1. The results of variance analysis indicated that the differences of infected leaf ratio, disease index and relative resistant index reached significantly levels (p<0.05) between different resistant types. The 88 germplasms were clustered into HR, MR, MS and HS groups by DPS data analyzing software. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

Fu Q.,Tobacco Research Institute of CAAS | Yang B.,Shanghai Tobacco Group Co. | Ge J.,Shanghai Tobacco Group Co. | Liu D.,China Tobacco Shandong Industrial Co. | And 2 more authors.
Tobacco Science and Technology | Year: 2014

In order to rapidly detect the major physical indices (weight per unit leaf area, filling value, thickness, equilibrium moisture content and tensile strength) of flue-cured tobacco with near infrared (NIR) spectroscopy technology, NIR mathematical models were developed with near infrared diffuse reflectance spectroscopy and stechiometry. The results showed that the root mean square errors of prediction (RMSEP) of weight per unit leaf area, thickness, tensile strength, filling value and equilibrium moisture content were 0.553, 0.0169, 0.149, 0.234, 0.277, respectively; their average relative deviation were 7.82%, 8.36%, 10.88%, 4.18%, 2.83%, respectively. The results of t-test indicated that there was no significant difference between predictive values and standard values, the developed NIR models could be used in the rapid detection of physical indices of batches of tobacco samples.

Meng K.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Sun L.,Tobacco Research Institute of CAAS | Chang A.,Tobacco Research Institute of CAAS | Zhang J.,China Tobacco Shandong Industrial Co.
Tobacco Science and Technology | Year: 2013

To collect the germplasm materials of tobacco resistant to powdery mildew (Erysiphe cichoracearum DC), 21 tobacco cultivars were selected and inoculated with the fungal isolate, which was kept by Tobacco Research Institute of Chinese Academy of Agricultural Sciences, in greenhouse in 2011. In 2012, 15 tobacco cultivars were selected and inoculated with an isolate of E. cichoracearum DC, which was collected from Sichuan, in an isolation chamber. The results demonstrated that sun-cured tobacco Tangpeng was immune to both isolates. NC89 showed high resistance to the disease. Beihart1000-1, K346 and Jiyan No.9 possessed medium resistance to powdery mildew. Zhongyan90 and CF203 were highly susceptible to the disease. Longjiang911, G140, TI245, Zhongyan100, Yunyan87, Yunyan85, Zhongyan103, Honghuadajinyuan, Cuibi No.1, G80, NC55, Nanjiang No.3 and Jingyehuang were medium or high susceptible to powdery mildew. Hicks showed different resistance to the two isolates with no obvious regularity.

Yang J.-G.,Tobacco Research Institute of CAAS | Wang F.-L.,Tobacco Research Institute of CAAS | Chen D.-X.,Tobacco Research Institute of CAAS | Shen L.-L.,Tobacco Research Institute of CAAS | And 4 more authors.
Sensors (Switzerland) | Year: 2012

Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV. In this study we report the development of an immunocapture real-time RT-PCR (IC-real-time RT-PCR) assay for direct detection of TMV in soils without RNA isolation. A series of TMV infected leaf sap dilutions of 1:101, 1:102, 1:103, 1:104, 1:105 and 1:106 (w/v, g/mL) were added to one gram of soil. The reactivity of DAS-ELISA and conventional RT-PCR was in the range of 1:102 and 1:103 dilution in TMV-infested soils, respectively. Meanwhile, the detection limit of IC-real-time RT-PCR sensitivity was up to 1:106 dilution. However, in plant sap infected by TMV, both IC-real-time RT-PCR and real-time RT-PCR were up to 1:106 dilution, DAS-ELISA could detect at least 1:103 dilution. IC-real-time RT-PCR method can use either plant sample extracts or cultivated soils, and show higher sensitivity than RT-PCR and DAS-ELISA for detection of TMV in soils. Therefore, the proposed IC-real-time RT-PCR assay provides an alternative for quick and very sensitive detection of TMV in soils, with the advantage of not requiring a concentration or RNA purification steps while still allowing detection of TMV for disease control. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

PubMed | University of Chinese Academy of Sciences, Yunnan Tobacco Company Dalizhou Branch and Tobacco Research Institute of CAAS
Type: Journal Article | Journal: Yi chuan = Hereditas | Year: 2016

The coding products of WRKY gene family plays important roles in plant growth and development as well as in various stress responses. They have been identified in various plants, but only few in common tobacco (Nicotiana tabacum L.). In this study, 164 putative WRKY proteins in the common tobacco genome were identified by using the conserved WRKY sequence (PF03106) from the Pfam database. Phylogenetic trees, functional domain analysis, chromosomal localization, subcellular localization and tissue expression patterns were analyzed with the bioinformatics softwares, including DNAMAN 5.0, Weblogo 3, MEGA 5.1, MG2C and MEME. First of all, phylogenetic trees divided all the candidate genes into three subfamilies: , and , respectively, and subfamily could be further divided into five subgroups: group -a, -b, -c, -d and -e. Secondly, the WRKY regions contained a highly conserved heptapeptide stretch WRKYGQK followed by a zinc-finger motif. Most of the NtWRKY genes contained 2-5 exons and a highly conserved gene structure. Thirdly, 154 out of 164 NtWRKY genes were distributed with different densities on 24 chromosomes, and each subfamily with different patterns and frequency. The largest number of NtWRKY genes was found on chromosome VI, and only one on chromosome X. Fourthly, the majority of NtWRKY members located in the nucleus, with 74 percent of subfamily in the extracellular matrix. Lastly, the members in the same subfamily had different spatial and temporal expression profiles, with 11 NtWRKY genes in roots, stems and leaves expressed at various levels. The expression of genes NtWRKY26, NtWRKY30 and NtWRKY32 can be induced by Phytophthora nicotianae. Our research thus provides valuable information for NtWRKY gene cloning and functional characterization in common tobacco.

Wang Z.,Liaocheng Hospital | Wang Z.,Liaocheng University | Yan Z.,Tobacco Research Institute of CAAS | Wang M.,Liaocheng Hospital | Zhao J.,Liaocheng Hospital
International Journal of Electrochemical Science | Year: 2015

The high cost and insufficient catalytic activity of carbon-supported Pt nanoparticle catalysts are two main problems in the commercial deployment of microbial fuel cell (MFC). In an effort to address these barriers, three carbon-supported Pt-Ni alloy electrocatalysts with varying Pt:Ni atom ratios (Pt3-Ni/C, Pt2-Ni/C, Pt-Ni/C) were prepared by simple NaBH4 reduction in glycerol at room temperature. All of the prepared Pt-Ni alloy nanoparticles (NPs) are highly dispersed on a carbon support and show a single-phase face-centered cubic structure with a particle size of 2.4-3.0 nm. The electrocatalytic performances of the synthesized Pt-Ni alloy catalysts were compared with that of commercial Pt/C by cyclic voltammetry and linear sweep voltammetry; among these NPs, the Pt2-Ni/C catalyst exhibits the highest activity for oxygen reduction reaction (ORR) in 0.1M HClO4. As the cathode catalyst, the maximum power density produced from MFC with Pt2-Ni/C (1724 mW m-2) was 22% higher than that with commercial Pt/C (1413 mW m-2). © 2015 The Authors.

PubMed | Qingdao Agricultural University and Tobacco Research Institute of CAAS
Type: Journal Article | Journal: Plant cell reports | Year: 2015

Nicotiana sylvestris calcineurin B-like protein NsylCBL10 improves tolerance to high-salt stress through better maintenance of Na (+) balance. The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa) and poplar (Populus trichocarpa), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10, a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris. NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na(+)/K(+) ratio in roots and higher Na(+)/K(+) ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N. sylvestris, by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell.

PubMed | Chinese Academy of Agricultural Sciences, Qingdao Agricultural University and Tobacco Research Institute of CAAS
Type: | Journal: International journal of genomics | Year: 2015

Abiotic stress halts the physiological and developmental process of plant. During stress condition, CBL-CIPK complex is identified as a primary element of calcium sensor to perceive environmental signals. Recent studies established that this complex regulates downstream targets like ion channels and transporters in adverse stages conditions. Crosstalks between the CBL-CIPK complex and different abiotic stresses can extend our research area, which can improve and increase the production of genetically modified crops in response to abiotic stresses. How this complex links with environmental signals and creates adjustable circumstances under unfavorable conditions is now one of the burning issues. Diverse studies are already underway to delineate this signalling mechanism underlying different interactions. Therefore, up to date experimental results should be concisely published, thus paving the way for further research. The present review will concisely recapitulate the recent and ongoing research progress of positive ions (Mg(2+), Na(+), and K(+)), negative ions (NO3 (-), PO4 (-)), and hormonal signalling, which are evolving from accumulating results of analyses of CBL and CIPK loss- or gain-of-function experiments in different species along with some progress and perspectives of our works. In a word, this review will give one step forward direction for more functional studies in this area.

Li Y.,Tobacco Research Institute of CAAS | Li Y.,Chinese Academy of Agricultural Sciences | Luo C.,Tobacco Research Institute of CAAS | Ren M.,Tobacco Research Institute of CAAS | And 4 more authors.
Tobacco Science and Technology | Year: 2014

To screen the genetic resources of flue-cured tobacco with high polyphenol content and investigate the differences of polyphenol content in tobacco of the same genotype under different ecological conditions, the polyphenol contents in flue-cured tobacco of 54 genotypes from Zhucheng in Shandong and Xichang in Sichuan were determined. The results showed that, both genotype and growing area ecological condition affected the polyphenol content in flue-cured tobacco greatly. The maximum absolute polyphenol content were 2 to 3 times as the minimum content in different genotypes, and the maximum relative chlorogenic acid and rutin contents were higher by 20 percentage points than that of the minimum. The varieties with the total polyphenol content higher than the average value in both areas included NC89, Jingyehuang, Zhongyan 102, Qinyan 95, Zhongyan 98, Yunyan 87, Zhongyan 14, Shanyu 2, Gexin 3, which could be used as the parent in high polyphenol content variety breeding. For the same genotype, the absolute contents of chlorogenic acid, rutin and total polyphenol in leaves from Sichuan were 1.5 to 2.0 times of those from Shandong; while the absolute scopoletin content in leaves from Shandong were nearly 2 times of those from Sichuan. Comparing with the leaves from Shandong, the polyphenol content in leaves from Sichuan featured higher absolute contents of chlorogenic acid and rutin and lower absolute content of scopoletin.

Xue D.,Tobacco Research Institute of CAAS | Chen D.,Tobacco Research Institute of CAAS | Wang X.,Tobacco Research Institute of CAAS | Zhao C.,Tobacco Research Institute of CAAS | Ren G.,Tobacco Research Institute of CAAS
Tobacco Science and Technology | Year: 2013

In order to ascertain the sex ratio of field population of Phthorimaea operculella, a method based on the morphology of abdominal segments of Phthorimaea operculella pupae was studied. The results showed that the female pupa had a long suture in the middle of the 8th abdominal segment, the suture extended to the 7th and 9th abdominal segments, it shaped as letter "Y" at the joint to the 7th abdominal segment. The long suture was formed by connecting the gonopore on the 8th abdominal segment with the oviporus on the 9th abdominal segment. The male pupa had no suture on the 8th abdominal segment, there was only a short longitudinal suture on the 9th abdominal segment. Effective pupa sexing is beneficial to monitoring dynamically the population occurrence of Phthorimaea operculella.

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