Tobacco Research Institute of CAAS

Qingdao, China

Tobacco Research Institute of CAAS

Qingdao, China
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Xiaohua X.,1 Tobacco Research Institute Of Caas | Xinru W.,Tobacco Research Institute of CAAS | Jiangtao C.,Tobacco Research Institute of CAAS | Minglei Y.,1 Tobacco Research Institute Of Caas | And 4 more authors.
Yi chuan = Hereditas | Year: 2016

The coding products of WRKY gene family plays important roles in plant growth and development as well as in various stress responses. They have been identified in various plants, but only few in common tobacco (Nicotiana tabacum L.). In this study, 164 putative WRKY proteins in the common tobacco genome were identified by using the conserved WRKY sequence (PF03106) from the Pfam database. Phylogenetic trees, functional domain analysis, chromosomal localization, subcellular localization and tissue expression patterns were analyzed with the bioinformatics softwares, including DNAMAN 5.0, Weblogo 3, MEGA 5.1, MG2C and MEME. First of all, phylogenetic trees divided all the candidate genes into three subfamilies: Ⅰ, Ⅱ and Ⅲ, respectively, and subfamily Ⅱ could be further divided into five subgroups: group Ⅱ-a, -b, -c, -d and -e. Secondly, the WRKY regions contained a highly conserved heptapeptide stretch WRKYGQK followed by a zinc-finger motif. Most of the NtWRKY genes contained 2-5 exons and a highly conserved gene structure. Thirdly, 154 out of 164 NtWRKY genes were distributed with different densities on 24 chromosomes, and each subfamily with different patterns and frequency. The largest number of NtWRKY genes was found on chromosome VI, and only one on chromosome X. Fourthly, the majority of NtWRKY members located in the nucleus, with 74 percent of subfamily Ⅲ in the extracellular matrix. Lastly, the members in the same subfamily had different spatial and temporal expression profiles, with 11 NtWRKY genes in roots, stems and leaves expressed at various levels. The expression of genes NtWRKY26, NtWRKY30 and NtWRKY32 can be induced by Phytophthora nicotianae. Our research thus provides valuable information for NtWRKY gene cloning and functional characterization in common tobacco.


Wang H.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Luo Z.,Tobacco Research Institute of CAAS | Hu Y.,China Tobacco Guangxi Industrial Co. | And 2 more authors.
Tobacco Science and Technology | Year: 2016

In order to obtain an effective antagonistic microorganism against tobacco black shank (Phytophthora parasitica var. nicotianae), a Penicillium stain QMYCS-2 was isolated by the spread plate method from rhizosphere soil of healthy tobacco plants. This strain was identified to be Penicillium madriti based on its morphological characteristics and ITS-rDNA sequence analysis. The results of confronting culture between QMYCS-2 and P. parasitica var. nicotianae showed that: 1) The inhibition rate of QMYCS-2 against the growth of P. parasitica var. nicotianae reached 71.76%. QMYCS-2 was cultured in five mediums for the fungi of plant pathogen, the inhibition effects of the obtained fermentation filtrate on P. parasitica var. nicotianae were determined. The result indicated that potato glucose liquid medium was the best culture medium. 2) The inhibition effects of QMYCS-2 fermentation filtrate and penicillin on the growth of P. nicotianae var. nicotianae were compared, the inhibition rate of the former was 64.89%-100.00%, while the highest of the latter was 7.33%. The control efficiency of QMYCS-2 fermentation filtrate against tobacco black shank in greenhouse was 73.25%, which was close to that of metalaxyl mancozeb (71.50%), a conventional fungicide used to control black shank in tobacco production. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Xue L.,Anhui Wannan Tobacco Co. | Zhu Q.,Anhui Wannan Tobacco Co. | Ji X.,Anhui Wannan Tobacco Co. | Zhang Z.,Tobacco Research Institute of CAAS | And 3 more authors.
Tobacco Science and Technology | Year: 2016

To establish the relationships of chemical components with sensory quality of flue-cured tobacco from south Anhui tobacco growing areas, 119 tobacco samples were collected from nine counties/districts there, and comprehensive statistical and correlation analyses of the chemical components and sensory quality of the leaf samples were conducted. The results showed that: 1) The average values of chemical components in these flue-cured tobacco were basically within the appropriate ranges required by quality tobacco leaves. 2) The sensory quality and burnt-sweetness character of tobacco positively correlated to total sugar, reducing sugar and the ratios of reducing sugar/total alkaloid, total sugar/total alkaloid and total nitrogen/nicotine in a statistically significant manner, while an extreme negative correlation existed for nicotine, total nitrogen, potassium, chlorine, and Shmuck value. 3) There was significant positive correlation between the burnt-sweetness character and the scores of overall sensory evaluation. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Luo Z.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Wang C.,China Tobacco Zhejiang Industrial Co. | Chen Q.,China Tobacco Zhejiang Industrial Co. | And 3 more authors.
Tobacco Science and Technology | Year: 2015

In order to clarify the resistance of Hubei origin germplasms of air-cured and sun-cured tobaccos to powdery mildew, 88 germplasms of air-cured and sun-cured tobacco were evaluated in greenhouse by spraying inoculation with the fungal isolate kept by Tobacco Research Institute of Chinese Academy of Agricultural Sciences. The results showed that the resistance to powdery mildew differed significantly, 13 were highly resistant (HR), 39 moderately resistant (MR), 18 moderately susceptible (MS), and 18 highly susceptible (HS) among the total 88 germplasms. The highest resistant ones were LC04, ZX06 and WF04, with relative resistant indexes of 0.99. The highest susceptible ones were FJY01 and CY08, with relative resistant indexes of less than 0.1. The results of variance analysis indicated that the differences of infected leaf ratio, disease index and relative resistant index reached significantly levels (p<0.05) between different resistant types. The 88 germplasms were clustered into HR, MR, MS and HS groups by DPS data analyzing software. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Fu Q.,Tobacco Research Institute of CAAS | Yang B.,Shanghai Tobacco Group Co. | Ge J.,Shanghai Tobacco Group Co. | Liu D.,China Tobacco Shandong Industrial Co. | And 2 more authors.
Tobacco Science and Technology | Year: 2014

In order to rapidly detect the major physical indices (weight per unit leaf area, filling value, thickness, equilibrium moisture content and tensile strength) of flue-cured tobacco with near infrared (NIR) spectroscopy technology, NIR mathematical models were developed with near infrared diffuse reflectance spectroscopy and stechiometry. The results showed that the root mean square errors of prediction (RMSEP) of weight per unit leaf area, thickness, tensile strength, filling value and equilibrium moisture content were 0.553, 0.0169, 0.149, 0.234, 0.277, respectively; their average relative deviation were 7.82%, 8.36%, 10.88%, 4.18%, 2.83%, respectively. The results of t-test indicated that there was no significant difference between predictive values and standard values, the developed NIR models could be used in the rapid detection of physical indices of batches of tobacco samples.


Meng K.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Sun L.,Tobacco Research Institute of CAAS | Chang A.,Tobacco Research Institute of CAAS | Zhang J.,China Tobacco Shandong Industrial Co.
Tobacco Science and Technology | Year: 2013

To collect the germplasm materials of tobacco resistant to powdery mildew (Erysiphe cichoracearum DC), 21 tobacco cultivars were selected and inoculated with the fungal isolate, which was kept by Tobacco Research Institute of Chinese Academy of Agricultural Sciences, in greenhouse in 2011. In 2012, 15 tobacco cultivars were selected and inoculated with an isolate of E. cichoracearum DC, which was collected from Sichuan, in an isolation chamber. The results demonstrated that sun-cured tobacco Tangpeng was immune to both isolates. NC89 showed high resistance to the disease. Beihart1000-1, K346 and Jiyan No.9 possessed medium resistance to powdery mildew. Zhongyan90 and CF203 were highly susceptible to the disease. Longjiang911, G140, TI245, Zhongyan100, Yunyan87, Yunyan85, Zhongyan103, Honghuadajinyuan, Cuibi No.1, G80, NC55, Nanjiang No.3 and Jingyehuang were medium or high susceptible to powdery mildew. Hicks showed different resistance to the two isolates with no obvious regularity.


PubMed | University of Chinese Academy of Sciences, Yunnan Tobacco Company Dalizhou Branch and Tobacco Research Institute of CAAS
Type: Journal Article | Journal: Yi chuan = Hereditas | Year: 2016

The coding products of WRKY gene family plays important roles in plant growth and development as well as in various stress responses. They have been identified in various plants, but only few in common tobacco (Nicotiana tabacum L.). In this study, 164 putative WRKY proteins in the common tobacco genome were identified by using the conserved WRKY sequence (PF03106) from the Pfam database. Phylogenetic trees, functional domain analysis, chromosomal localization, subcellular localization and tissue expression patterns were analyzed with the bioinformatics softwares, including DNAMAN 5.0, Weblogo 3, MEGA 5.1, MG2C and MEME. First of all, phylogenetic trees divided all the candidate genes into three subfamilies: , and , respectively, and subfamily could be further divided into five subgroups: group -a, -b, -c, -d and -e. Secondly, the WRKY regions contained a highly conserved heptapeptide stretch WRKYGQK followed by a zinc-finger motif. Most of the NtWRKY genes contained 2-5 exons and a highly conserved gene structure. Thirdly, 154 out of 164 NtWRKY genes were distributed with different densities on 24 chromosomes, and each subfamily with different patterns and frequency. The largest number of NtWRKY genes was found on chromosome VI, and only one on chromosome X. Fourthly, the majority of NtWRKY members located in the nucleus, with 74 percent of subfamily in the extracellular matrix. Lastly, the members in the same subfamily had different spatial and temporal expression profiles, with 11 NtWRKY genes in roots, stems and leaves expressed at various levels. The expression of genes NtWRKY26, NtWRKY30 and NtWRKY32 can be induced by Phytophthora nicotianae. Our research thus provides valuable information for NtWRKY gene cloning and functional characterization in common tobacco.


PubMed | Qingdao Agricultural University and Tobacco Research Institute of CAAS
Type: Journal Article | Journal: Plant cell reports | Year: 2015

Nicotiana sylvestris calcineurin B-like protein NsylCBL10 improves tolerance to high-salt stress through better maintenance of Na (+) balance. The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa) and poplar (Populus trichocarpa), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10, a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris. NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na(+)/K(+) ratio in roots and higher Na(+)/K(+) ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N. sylvestris, by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell.


PubMed | Chinese Academy of Agricultural Sciences, Qingdao Agricultural University and Tobacco Research Institute of CAAS
Type: | Journal: International journal of genomics | Year: 2015

Abiotic stress halts the physiological and developmental process of plant. During stress condition, CBL-CIPK complex is identified as a primary element of calcium sensor to perceive environmental signals. Recent studies established that this complex regulates downstream targets like ion channels and transporters in adverse stages conditions. Crosstalks between the CBL-CIPK complex and different abiotic stresses can extend our research area, which can improve and increase the production of genetically modified crops in response to abiotic stresses. How this complex links with environmental signals and creates adjustable circumstances under unfavorable conditions is now one of the burning issues. Diverse studies are already underway to delineate this signalling mechanism underlying different interactions. Therefore, up to date experimental results should be concisely published, thus paving the way for further research. The present review will concisely recapitulate the recent and ongoing research progress of positive ions (Mg(2+), Na(+), and K(+)), negative ions (NO3 (-), PO4 (-)), and hormonal signalling, which are evolving from accumulating results of analyses of CBL and CIPK loss- or gain-of-function experiments in different species along with some progress and perspectives of our works. In a word, this review will give one step forward direction for more functional studies in this area.


Xue D.,Tobacco Research Institute of CAAS | Chen D.,Tobacco Research Institute of CAAS | Wang X.,Tobacco Research Institute of CAAS | Zhao C.,Tobacco Research Institute of CAAS | Ren G.,Tobacco Research Institute of CAAS
Tobacco Science and Technology | Year: 2013

In order to ascertain the sex ratio of field population of Phthorimaea operculella, a method based on the morphology of abdominal segments of Phthorimaea operculella pupae was studied. The results showed that the female pupa had a long suture in the middle of the 8th abdominal segment, the suture extended to the 7th and 9th abdominal segments, it shaped as letter "Y" at the joint to the 7th abdominal segment. The long suture was formed by connecting the gonopore on the 8th abdominal segment with the oviporus on the 9th abdominal segment. The male pupa had no suture on the 8th abdominal segment, there was only a short longitudinal suture on the 9th abdominal segment. Effective pupa sexing is beneficial to monitoring dynamically the population occurrence of Phthorimaea operculella.

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