Tlajomulco de Zuniga, Mexico

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PubMed | Instituto Tecnológico de Tuxtla Gutierrez and Tlajomulco Jalisco Institute of Technology
Type: Journal Article | Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] | Year: 2016

The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p<0.05) on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.


Gurrola-Diaz C.M.,Centro Universitario Of Ciencias Of La Salud | Garcia-Lopez P.M.,University of Guadalajara | Sanchez-Enriquez S.,Centro Universitario Of Ciencias Of La Salud | Troyo-Sanroman R.,Centro Universitario Of Ciencias Of La Salud | And 2 more authors.
Phytomedicine | Year: 2010

Insulin resistance, obesity, hypertension, and dyslipidemia are strongly associated with metabolic syndrome (MeSy), which is considered to be a reversible clinical stage before its evolution to coronary heart disease and diabetes. Currently, the antihypertensive and hypolipidemic properties of aqueous Hibiscus sabdariffa extracts (HSE) have been demonstrated in clinical trials and in vivo experiments. The aim of the present study was to evaluate the effects of a Hibiscus sabdariffa extract powder (HSEP) and a recognized preventive treatment (diet) on the lipid profiles of individuals with and without MeSy according to the National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) criteria. The protocol was a follow-up study carried out in a factorial, randomized design (T1=preventive treatment comprises Diet, T2=HSEP, T3=HSEP+preventive treatment (Diet) X MeSy, non-MeSy individuals). A total daily dose of 100 mg HSEP was orally administered in capsules for one month. The preventive treatment (diet) was selected according to NCEP-ATP III recommendations and adjusted individually. Total cholesterol, LDL-c, HDL-c, VLDL-c, triglycerides, glucose, urea, creatinine, AST, and ALT levels in the blood were determined in all individuals pre- and post-treatment. The MeSy patients treated with HSEP had significantly reduced glucose and total cholesterol levels, increased HDL-c levels, and an improved TAG/HDL-c ratio, a marker of insulin resistance (t-test p<0.05). Additionally, a triglyceride-lowering effect was observed in MeSy patients treated with HSEP plus diet, and in individuals without MeSy treated with HSEP. Significant differences in total cholesterol, HDL-c, and the TAG/HDL-c ratio were found when the means of absolute differences among treatments were compared (ANOVA p<0.02). Therefore, in addition to the well documented hypotensive effects of Hibiscus sabdariffa, we suggest the use of HSEP in individuals with dyslipidemia associated with MeSy. © 2009 Elsevier GmbH. All rights reserved.


Vega-Ramos K.L.,Azul Agricultura y Servicios S.A. de C.V. | Uvalle-Bueno J.X.,Azul Agricultura y Servicios S.A. de C.V. | Gomez-Leyva J.F.,Tlajomulco Jalisco Institute of Technology
Biochemical Genetics | Year: 2013

In this study, 115 isolates of Fusarium oxysporum from roots of Agave tequilana Weber cv azul plants and soil in commercial plantations in western Mexico were characterized using morphological and molecular methods. Genetic analyses of monosporic isolates included restriction enzyme analysis of rDNA (ARDRA) using HaeIII and HinfI, and genetic diversity was determined using Box-PCR molecular markers. Box-PCR analysis generated 14 groups. The groups correlated highly with the geographic location of the isolate and sample type. These results demonstrate the usefulness of ARDRA and Box-PCR techniques in the molecular characterization of the Fusarium genus for the discrimination of pathogenic isolates. © 2013 Springer Science+Business Media New York.


Fajardo-Delgado D.,Tlajomulco Jalisco Institute of Technology | Fernandez-Zepeda J.A.,Research Center Cientifica Educacion Superior Of Ensenada | Bourgeois A.G.,Georgia State University
IEEE Transactions on Parallel and Distributed Systems | Year: 2013

In this paper, we introduce the Bodyguard Allocation Problem (BAP) game, that illustrates the behavior of processes with contradictory individual goals in distributed systems. In particular, the game deals with the conflict of interest between two classes of processes that maximize/minimize their distance to a special process called the root. A solution of the BAP game represents a rooted spanning tree in which there exists a condition of equilibrium with maximum social welfare. We analyze the inefficiency of equilibria of the game based on both a completely cooperative and noncooperative approach. Additionally, we design two algorithms, CBAP and DBAP, that provide approximated solutions for the BAP game. We prove that both algorithms always terminate in a configuration with equilibrium and we analyze their running time based on the approach of cooperation used. We perform experimental simulations to compare the overall quality of equilibria obtained by the proposed algorithms. © 1990-2012 IEEE.


PubMed | Research Center Estudios Avanzados Del, Tlajomulco Jalisco Institute of Technology and Instituto Nacional de Investigaciones Nucleares
Type: | Journal: Planta | Year: 2016

An amaranth DGR gene, induced under abiotic stress, modifies cell wall structure and causes hypersensitivity to ABA and salt when overexpressed in Arabidopsis. DUF642 is a highly conserved plant-specific family of unknown cell wall-associated proteins. The AhDGR2 gene, coding for a DUF642 protein, was significantly induced in grain amaranth (Amaranthus hypochondriacus) plants subjected to water-deficit and salinity stress, thereby suggesting its participation in abiotic stress tolerance in this plant. A role in development was also inferred from the higher AhDGR2 expression rates detected in young tissues. Subsequent overexpression of AhDGR2 in transgenic Arabidopsis plants (OE-AhDGR2) supported its possible role in development processes. Thus, OE-AhDGR2 plants generated significantly longer roots when grown in normal MS medium. However, they showed a hypersensitivity to increasing concentrations of abscisic acid or NaCl in the medium, as manifested by shorter root length, smaller and slightly chlorotic rosettes, as well as highly reduced germination rates. Contrary to expectations, OE-AhDGR2 plants were intolerant to abiotic stress. Moreover, cell walls in transgenic plants were thinner, in leaves, and more disorganized, in roots, and had significantly modified pectin levels. Lower pectin methylesterase activity detected in leaves of OE-AhDGR2 plants, but not in roots, was contrary to previous reports associating DUF642 proteins and decreased pectin esterification levels in cell walls. Nonetheless, microarray data identified candidate genes whose expression levels explained the phenotypes observed in leaves of OE-AhDGR2 plants, including several involved in cell wall integrity and extension, growth and development, and resistance to abiotic stress. These results support the role of DUF642 proteins in cell wall-related processes and offer novel insights into their possible role(s) in plants.


Valenzuela-Zapata A.G.,Signo Tequila Foundation | Lopez-Muraira I.,Tlajomulco Jalisco Institute of Technology | Gaytan M.S.,University of Utah
Ethnobiology Letters | Year: 2011

The Mexican sport of charrería, or Mexican rodeo, developed in post-conquest Mexico as a way of preserving and celebrating traditional cowboy riding and livestock handling skills. Today, charrería is considered the national sport of Mexico and the charro (cowboy) is also a celebrated icon of Mexicanness. Special handcrafted ropes used in charrería, known as sogas finas, or charro lariats, are made from the fibers of the Agave inaequidens. The manufacture of charro ropes is an artisinal practice that requires both cultural and botanical knowlege. In the last ten years, there has been a significant decline in the A. inaequidens population in the Cerro Viejo mountain range of the central-western Mexican state of Jalisco, putting the financial wellbeing of local lariat artisans at risk. Drawing on fieldwork and laboratory analysis conducted from 2002 through 2010, we discuss the socio-cultural significance of charro lariats, detail the harvesting of A. inaequidens in relation to lariat craftsmanship, document the physical characteristics of the A. inaequidens from this region, and describe the relationship between traditional knowledge and the local economy. The goal of this research is two-fold: 1) to stimulate feedback between producers and consumers in an attempt to leverage the existing business cluster based on traditional knowledge and 2) to initiate dialogue concerning conservation, domestication, and sustainable management of the wild A. inadequidens population. © 2011 Society of Ethnobiology.


PubMed | National Autonomous University of Mexico, Tlajomulco Jalisco Institute of Technology, University of Colima, Institute Investigacion Lightbourn and San Luis Potosí Institute of Scientific Research and Technology
Type: | Journal: Food chemistry | Year: 2015

Chan (Hyptis suaveolens) is a Mesoamerican crop highly appreciated since the pre-Hispanic cultures. Its proteins are a good source of essential amino acids; however, there are no reports on the properties of its individual proteins. In this study, the 11S globulin (Hs11S) was purified and biochemically characterized. The molecular weight of native Hs11S was about 150-300 kDa with isoelectric points of 5.0-5.3, composed by four monomers of 53.5, 52, 51.1 and 49.5 kDa, each formed by one acidic subunit and one basic subunit linked by a disulfide bond. Dynamic light scattering, size exclusion chromatography and native PAGE show that Hs11S is assembled in different oligomeric forms. LC-MS/MS analysis confirmed its identity. Hs11S presents antigenic determinants in common with lupin 11S globulin. Carbohydrate moieties or phosphate groups linked to Hs11S were not detected. This information is very useful in order to exploit and utilize rationally chan 11S globulin in food systems.


Virgen-Ortiz J.J.,University of Colima | Ibarra-Junquera V.,University of Colima | Osuna-Castro J.A.,University of Colima | Escalante-Minakata P.,University of Colima | And 2 more authors.
Analytical Biochemistry | Year: 2012

One of the recurrent methodological problems in preparative biochemical work is the concentration of dilute protein solutions, including culture supernatants resulting from biotechnological processes. A procedure was developed to concentrate enzymes by a novel cryoconcentration system. This approach includes a new device that facilitates the sample freezing and the subsequent solute elution from the frozen matrix by centrifugation. The optimal centrifugation conditions for this cryoconcentration system were obtained using whey protein solution as a model. The procedure was applied to concentrate dilute solutions of commercial pectinase, measuring the endopolygalacturonase (EPG) activity of this enzyme in the concentrate by a method based on the on-line torque measurement, and of recombinant fructan:fructan 1-fructosyltransferase (1-FFT) protein of Pichia pastoris from a culture in a bioreactor, as an expression system. The optimal centrifugation speed, time, and temperature were 6150 g, 20 min, and 4 °C, respectively. The concentration factors for the dilute protein solutions were 9.2-, 11.2-, and 17.1-fold for 1-FFT, whey, and commercial pectinase, respectively. Recoveries ranged from 87% to 93%. The procedure allowed concentrating proteins efficiently without affecting their enzymatic activity. © 2012 Elsevier Inc. All rights reserved.


Virgen-Ortiz J.J.,University of Colima | Ibarra-Junquera V.,University of Colima | Escalante-Minakata P.,University of Colima | Osuna-Castro J.A.,University of Colima | And 3 more authors.
Analytical Biochemistry | Year: 2013

This work presents a rapid and simple freeze centrifugation method to concentrate dilute protein solutions for detection by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) Coomassie blue staining. Moreover, a simple way to assemble a cryoconcentration device is presented, and its use is discussed. Commercial purified protein standard and an enzyme with high fructosyltransferase (FTase) activity, coming from target fractions obtained by chromatographic separation, were used as an example. FTase, coming directly from the chromatographic fractions, was difficult to view through SDS-PAGE analysis; however, it was easily visualized, and its activity was enhanced, after the application of the freeze centrifugation protocol presented here. © 2013 Elsevier Ltd. All rights reserved.


Suarez-Gonzalez E.M.,Tlajomulco Jalisco Institute of Technology | Lopez M.G.,CINVESTAV | Delano-Frier J.P.,CINVESTAV | Gomez-Leyva J.F.,Tlajomulco Jalisco Institute of Technology
Journal of Plant Physiology | Year: 2014

The expression of genes coding for sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and fructan:fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100), both fructan biosynthesizing enzymes, characterization by TLC and HPAEC-PAD, as well as the quantification of the fructo-oligosaccharides (FOS) accumulating in response to the exogenous application of sucrose, kinetin (cytokinin) or other plant hormones associated with (a)biotic stress responses were determined in two Agave species grown in vitro, domesticated Agave tequilana var. azul and wild A. inaequidens. It was found that elicitors such as salicylic acid (SA), and jasmonic acid methyl ester (MeJA) had the strongest effect on fructo-oligosaccharide (FOS) accumulation. The exogenous application of 1mM SA induced a 36-fold accumulation of FOS of various degrees of polymerization (DP) in stems of A. tequilana. Other treatments, such as 50mM abscisic acid (ABA), 8% Sucrose (Suc), and 1.0mgL-1 kinetin (KIN) also led to a significant accumulation of low and high DP FOS in this species. Conversely, treatment with 200μM MeJA, which was toxic to A. tequilana, induced an 85-fold accumulation of FOS in the stems of A. inaequidens. Significant FOS accumulation in this species also occurred in response to treatments with 1mM SA, 8% Suc, and 10% polyethylene glycol (PEG). Maximum yields of 13.6 and 8.9mg FOS per g FW were obtained in stems of A. tequilana and A. inaequidens, respectively. FOS accumulation in the above treatments was tightly associated with increased expression levels of either the 1-FFT or the 1-SST gene in tissues of both Agave species. © 2013 Elsevier GmbH.

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