Bhagalpur, India

Tilka Manjhi Bhagalpur University, also known as Bhagalpur University, is a public university in Bhagalpur, Bihar, India. The university is named after famous freedom fighter, Tilka Manjhi. The university was established on 12 July 1960, having local colleges initially associated to Patna University. Its campus is around 264 acres of lush green area. The university has more than 36 teaching departments, around 24 affiliated colleges, and four research centers. The university is fully accredited to National Assessment and Accreditation Council and has tied with major international universities such as University of Vienna, the University of Bristol, the University of Kent, University of Lyon, University of Notre Dame, etc. Wikipedia.


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Jha A.K.,Aryabhatta Knowledge University | Prasad K.,Tilka Manjhi Bhagalpur University
Journal of Bionanoscience | Year: 2013

The process of development of reliable and green method for the synthesis of metallic nanoparticles is an important step in the field of nanotechnology. A novel rose (Rosa sp.) petals negotiated synthesis of gold nanoparticles is reported. X-ray, selected area electron diffraction pattern and lattice fringes indicated that the gold nanoparticles have face centered cubic crystalline structure. Transmission electron microscopy and energy dispersive X-ray analyses are performed to ascertain the formation of gold nanoparticles. Nanoparticles almost spherical in shape having a size of 3-15 nm are found. The reaction protocol was monitored using UV-visible spectroscopy. Accordingly, the entire span of experimentation has been proposed in the form of a mathematical model in the light optical dependence of maturing gold nanoparticles. Also, UV-visible study revealed the surface plasmon resonance at 539 nm. A possible involved mechanism for the biosynthesis of gold nanoparticles has also been discussed. Moreover, present biosynthetic method for the synthesis of gold nanoparticles is simple, easy to perform, pollutant free and inexpensive. Copyright © 2013 American Scientific Publishers. All rights reserved.


Jha A.K.,Tilka Manjhi Bhagalpur University | Prasad K.,Central University of Jharkhand
Digest Journal of Nanomaterials and Biostructures | Year: 2011

A rapid way of synthesizing silver nanoparticles by treating silver ions with a green Capsicum annum L. alcoholic fruit extract containing principally polyphenols, pigments, capsaicin and ascorbic acid is reported. The reaction protocol was monitored using UVvisible spectroscopy. Accordingly, the entire span of experimentation has been proposed in the form of a mathematical model in the light optical dependence of maturing Ag nanoparticles. The crystalline morphology and phase of the nanoparticles were determined with the help of transmission electron microscopy, selected area electron diffraction and X-ray diffraction studies. Nanoparticles almost spherical in shape having a size of 2-6 nm are found. The results indicated that the extract, which has capsaicin containing amine groups along with other candidate polyphenols and ascorbic acid, played a reducing and controlling role during the formation of silver nanoparticles in the solutions of green Capsicum annuum L. fruit.


Shahbaz Ullah M.,Tilka Manjhi Bhagalpur University | Hassan M.R.,Sm College
Astrophysics and Space Science | Year: 2014

In this manuscript we have established averaged equation of motion of the Sitnikov restricted three- body and four-body problem when all the primaries are point masses, by applying the Van der Pol transformation and averaging technique of J. Guckenheimer and P. Holmes (in Nonlinear Oscillations, Dynamical System Bifurcations of Vector Fields, Springer, Berlin, 1983). In addition to the resonance criterion at the 3/2 commensurability we have chosen ω=2n/3,n=4, ω is the angular velocity of the coordinate system. Further we established the Series solution of the three-body and four-body problem in the sense of Sitnikov. Lastly the periodicities of the solutions have been examined by the Poincare section and four-body and three-body problem have been compared by different comparative graphs and surfaces. © 2014 Springer Science+Business Media Dordrecht.


Jha A.K.,Tilka Manjhi Bhagalpur University | Prasad K.,Tilka Manjhi Bhagalpur University
Biotechnology Journal | Year: 2010

Green, low-cost, and reproducible Lactobacillus-mediated biosynthesis of metal and oxide nanoparticles are reported. Silver and titanium dioxide nanoparticles are synthesized using Lactobacillus sp. procured from yoghurt and probiotic tablets. The synthesis is performed akin to room temperature in the laboratory ambience. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of metallic and oxide nanoparticles. Individual nanoparticles having the dimensions of 10-25 nm (n-Ag) and 10-70 nm (n-TiO2) are found. The mechanism involved for the synthesis of metallic and oxide nanoparticles has also been discussed. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Bhagat S.,Tilka Manjhi Bhagalpur University | Prasad K.,Tilka Manjhi Bhagalpur University
Physica Status Solidi (A) Applications and Materials Science | Year: 2010

Lead-free perovskite Ba(Fe 1/2Nb 1/2)O 3 (BFN) was prepared by conventional ceramic fabrication technique at 1200 °C/5 h in air atmosphere. The crystal symmetry, space group, and unit cell dimensions were determined from the experimental results using FullProf software whereas crystallite size and lattice strain were estimated from Williamson-Hall approach. X-ray diffraction (XRD) analysis of the compound indicated the formation of a single-phase monoclinic structure with the space group P2/m. EDAX and SEM studies were carried out in order to evaluate the quality and purity of the compound. To find a correlation between the response of the real system and idealized model circuit composed of discrete electrical components, the model fittings were presented using the impedance data. Complex impedance analyses suggested the dielectric relaxation to be of non-Debye type. The correlated barrier hopping (CBH) model was employed to successfully explain the mechanism of charge transport in BFN. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Jha A.K.,Tilka Manjhi Bhagalpur University | Prasad K.,Tilka Manjhi Bhagalpur University
Colloids and Surfaces B: Biointerfaces | Year: 2010

A new low-cost, green and reproducible Lactobacillus sp. assisted biosynthesis of BaTiO3 nanoparticles is reported. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of BaTiO3 nanoparticles. The apparent crystallite size and lattice strain are estimated from Williamson-Hall approach. XRD analysis of the compound indicated the formation of a single-phase tetragonal structure. Individual nanoparticles as well as a few aggregate having the size of 20-80 nm are found. A possible involved mechanism for the biosynthesis of nano-BaTiO3 has also been proposed in which ROS as well as partial pressure of gaseous hydrogen (rH2) of the culture solution seems to play an important role in the process. Remarkable enhancement in dielectric properties was observed in BaTiO3/polyvinylidene fluoride (PVDF) nanocomposite. © 2009 Elsevier B.V. All rights reserved.


Prasad K.,Tilka Manjhi Bhagalpur University | Jha A.K.,Tilka Manjhi Bhagalpur University
Journal of Colloid and Interface Science | Year: 2010

A low-cost green and reproducible microbes (Lactobacillus sp. and Sachharomyces cerevisiae) mediated biosynthesis of CdS nanoparticles is reported. The synthesis is performed akin to room temperature in the laboratory ambience. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of CdS nanoparticles. Individual nanoparticles as well as a few aggregate having the size of 2.5-5.5 nm are found. UV-vis spectroscopy study revealed the surface plasmon resonance at 393 and 369 nm respectively for Lactobacillus and yeast assisted synthesis of CdS nanoparticles. The absorbance spectra were used to estimate the values of optical band gap and particle size of CdS nanoparticles. A possible involved mechanism for the biosynthesis of CdS nanoparticles has also been proposed. © 2009 Elsevier Inc. All rights reserved.


Jha A.K.,Tilka Manjhi Bhagalpur University | Prasad K.,Tilka Manjhi Bhagalpur University
International Journal of Green Nanotechnology: Physics and Chemistry | Year: 2010

A green, low-cost, and reproducible Cycas leaf-negotiated synthesis of silver nanoparticles is reported. X-ray and transmission electron microscopy (TEM) analyses are performed to ascertain the formation of Ag nanoparticles. Nanoparticles almost spherical in shape having the size of 2-6 nm are found. Rietveld analysis to the X-ray data indicated that Ag nanoparticles have fcc unit cell structure. Ultraviolet (UV)-visible study revealed the surface plasmon resonance at 449 nm. An effort has been made to understand the possible involved mechanism for the biosynthesis of Ag nanoparticles. Present procedure offers the benefit of eco-friendliness and amenability for large-scale production through scaling up. © 2009 Copyright Taylor and Francis Group, LLC.


Das D.K.,Tilka Manjhi Bhagalpur University | Rahman A.,Tilka Manjhi Bhagalpur University
Plant Cell, Tissue and Organ Culture | Year: 2012

To enhance the antifungal response of litchi (Litchi chinensis Sonn.), transgenic plants were generated by transferring rice chitinase gene driven by a maize-ubiquitin promoter along with its first intron into the zygotic embryos via Agrobacterium tumefaciens-mediated transformation. After co-cultivation for 2 days, zygotic embryos were transferred onto Murashige and Skoog (MS) modified media supplemented with 25 mgl -1 hygromycin and 400 mgl -1 cefotaxime. Consequently embryos were selected and the antibiotic resistant transgenic plantlets were regenerated. The culture time from zygotic embryo to transgenic plants was 14 months. The integration of the transgene was confirmed by PCR, RT-PCR, Southern and western blot analyses. The transgenic plants exhibited higher chitinase activity than the non-transformed plants. The chitinase activity when examined using the native polyacrylamide in-gel assay, indicated that the foreign gene expression resulting in the protein of expected molecular weight that showed chitinase activity. The transgenic plants showed delayed onset of the disease and smaller lesions following in vitro inoculation of die-back, leaf spots and blight pathogen (Phomopsis sp.). The transgenic plants were adapted to the greenhouse and did not show any phenotypic alterations. © 2012 Springer Science+Business Media B.V.


To enhance the antifungal response of blackgram (Vigna mungo L.), transgenic plants were generated by transferring bacterial chitinase gene with a CaMV 35S promoter. The chopped multiple shoot cells developed on the cotyledonary node were transformed by Particle gun method. The calli were raised on the Murashige and Skoog (MS) modified media supplemented with 50mgl−1 kanamycin. The transformation efficiency was 13 %. The resultant shoot buds were selected and the antibiotic resistant transgenic plantlets were regenerated. The development of the transgenic plants from the shoot buds took about four to six months. The transgenic status was confirmed by PCR, RT-PCR, Southern and western blot analyses. The transgenic plants exhibited higher chitinase activity than the non-transformed plants. The chitinase activity was examined using the native polyacrylamide in-gel assay. The transgenic plants showed enhanced resistance as evidenced by the delayed onset of the disease and smaller lesions following an in vitro inoculation of the powdery mildew pathogen (E. polygoni DC). The transgenic plants adapted well to the greenhouse and did not show any phenotypic alterations. © 2016 Prof. H.S. Srivastava Foundation for Science and Society

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