Tibet Vocational Technical College

Lhasa, China

Tibet Vocational Technical College

Lhasa, China

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Guo C.,Sun Yat Sen University | Huang X.-Y.,Sun Yat Sen University | Yang M.-J.,Sun Yat Sen University | Yang M.-J.,Tibet Vocational Technical College | And 4 more authors.
Fish and Shellfish Immunology | Year: 2014

Microbial disease problems constitute the largest single cause of economic losses in aquaculture. An understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. In the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by Edwardsiella tarda EIB202. We establish the metabolic profile of crucian carps and then compare the metabolic difference between survivals and dead fish by self-control. We identify elevating unsaturated fatty acid biosynthesis and decreasing fructose and mannose metabolism as the most key pathways and increasing palmitic acid and decreasing d-mannose as the most crucial metabolites differentiating survivals from death in these fish infected by E.tarda. Our findings highlight the importance of metabolic strategy against bacterial infections. © 2014 Elsevier Ltd.


Ma Y.-M.,Sun Yat Sen University | Yang M.-J.,Tibet Vocational Technical College | Wang S.,Xiamen University | Li H.,Sun Yat Sen University | Peng X.-X.,Sun Yat Sen University
Fish and Shellfish Immunology | Year: 2015

Streptococcus iniae seriously affects the intensive farming of tilapias. Much work has been conducted on prevention and control of S. iniae infection, but little published information on the metabolic response is available in tilapias against the bacterial infection, and no metabolic modulation way may be adopted to control this disease. The present study used GC/MS based metabolomics to characterize the metabolic profiling of tilapias infected by a lethal dose (LD50) of S. iniae and determined two characteristic metabolomes separately responsible for the survival and dying fishes. A reversal changed metabolite, decreased and increased l-leucine in the dying and survival groups, respectively, was identified as a biomarker which featured the difference between the two metabolomes. More importantly, exogenous l-leucine could be used as a metabolic modulator to elevate survival ability of tilapias infected by S. iniae. These results indicate that tilapias mount metabolic strategies to deal with bacterial infection, which can be regulated by exogenous metabolites such as l-leucine. The present study establishes an alternative way, metabolic modulation, to cope with bacterial infections. © 2015 Elsevier Ltd.


PubMed | National Engineering Research Center for Miniaturized Detection Systems, Northwest University, China, Minzu University of China and Tibet Vocational Technical College
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Gout is the most common form of inflammatory arthritis affecting men, and current evidence suggests that genetic factors contribute to its progression. As a previous study identified that WD40 repeat protein 1 (WDR1) is associated with gout in populations of European descent, we sought to investigate its relationship with this disease in the Han Chinese population. We genotyped six WDR1 single nucleotide polymorphisms in 143 gout cases and 310 controls using Sequenom MassARRAY technology. The SPSS 16.0 software was used to perform statistical analyses. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression, with adjustments for age and gender. In an analysis using an allelic model, we identified that the minor alleles of rs3756230 (OR = 0.64, 95%CI = 0.450-0.911, P = 0.013) and rs12498927 (OR = 1.377, 95%CI = 1.037-1.831, P = 0.027) were associated with gout risk. In addition, we found that the A/A genotype of rs12498927 was associated with increased risk of gout under codominant (OR = 2.22, 95%CI = 1.12- 4.40, P = 0.042) and recessive models (OR = 2.24, 95%CI = 1.20-4.17, P = 0.012). We also determined the A/G genotype of rs12498927 to be significantly associated with higher urea levels in gout patients (P = 0.017). Our data shed new light on the association between genetic variations in the WDR1 gene and gout susceptibility in the Han Chinese population.


PubMed | National Engineering Research Center for Miniaturized Detection Systems, Northwest University, China, Minzu University of China and Tibet Vocational Technical College
Type: Journal Article | Journal: Clinical rheumatology | Year: 2016

Gout is a common type of arthritis that is characterized by hyperuricemia, tophi, and joint inflammation. Current evidence suggests that heredity contributes to the progression of gout. Previous studies have shown that regulation of the ATP-binding cassette subfamily G member 2 (ABCG2) pathways plays a role in gout occurrence. To investigate and validate potential genetic associations with the risk of gout, we conducted a case-control study. We conducted 143 cases and 310 controls and genotyped seven single-nucleotide polymorphisms (SNPs) in ABCG2 gene. ABCG2 SNP association analyses were performed using SPSS 17.0 Statistical Package, PLINK Software, HaploView software package, and SHEsis software platform. We identified that four susceptibility SNPs were potentially associated with occurrence of gout. Rs2622621 and rs3114018 in ABCG2 can actually increase the risk of gout in log-additive model (rs2622621, odds ratio (OR)=1.90, 95% confidence interval (CI) 1.39-2.61, p<0.001; rs3114018, OR=1.55, 95% CI 1.13-2.13, p=0.006). We found that rs17731799G/T-G/G and rs3114020 T/C-T/T in ABCG2 can actually increase the risk of gout in dominant model (rs17731799, OR=1.67, 95% CI 1.05-2.66, p=0.028; rs3114020, OR=1.58, 95% CI 1.00-2.51, p=0.048). The ABCG2 haplotype GGCTCTC (OR=0.46, 95% CI 0.28-0.75, p=0.0019) decreased the gout risk. Our results, combined with those from previous studies, suggest that genetic variation in ABCG2 may influence gout susceptibility in the Han population.


PubMed | National Engineering Research Center for Miniaturized Detection Systems, Northwest University, China, Minzu University of China and Tibet Vocational Technical College
Type: Journal Article | Journal: Drug metabolism and pharmacokinetics | Year: 2016

Polymorphic distributions of pharmacogenes among some ethnicities are under-represented in current pharmacogenetic research. Particularly, there is a paucity of pharmacogenetic information in the Sherpa population in Tibet. We used the Sequenom MassARRAY single nucleotide polymorphism (SNP) genotyping technology to detect 86 very important pharmacogene (VIP) variants in Sherpas and compared the genotypic frequencies of these variants with HapMap populations. Overall, 59 of the 60 previously reported variants in the HapMap populations were found in our study. We found minimal differences between populations of Sherpas and Chinese Han in Beijing (CHB), Chinese in Metropolitan Denver, Colorado (CHD), Japanese in Tokyo, Japan (JPT), and Mexicans in Los Angeles, California (MEX) after a strict Bonferroni correction. Only 8, 4, 5, 4 VIP genotypes, respectively, were different in these groups. Additionally, pairwise FST values and clustering analyses showed that the VIP variants in the Sherpa population exhibited a close genetic affinity with the CHB and JPT populations, but they were most similar to the CHD population. Our results contribute to a better understanding of the molecular basis underlying ethnic differences in drug response, which may potentially benefit the development of personalized medicine for the Sherpa population.


Dou X.J.,Tibet Vocational Technical College | Zhang Y.,Shenzhen University | Wu Y.H.,Shenzhen University
Toxicology and Environmental Health Sciences | Year: 2012

48 Balb/C mice were exposed to different concentrations of gaseity formaldehyde. The neurotoxicity of formaldehyde was revealed at six aspects including step-down test, passive avoidance test, glial fibrillary acidic protein (GFAP) immunohistochemistry, activity of superoxide dismulase (SOD), content of malondialdehyde (MDA) and cell gel electrophoresis technology (SCGE). In step-down test and passive avoidance test, the mice exposed to formaldehyde at high dose and moderate dose has made notable errors, compared with control group (P<0. 05). The GFAPpositive cells were increased with the increasing of doses. Activity of SOD and content of MDA were significant reduced after exposures at moderate dose and high dose (P<0. 05). Formaldehyde can also induce to DNA tail length, tail moments and tail DNA changes with the increased dose. The data suggested the necessity for a systematic observance of precautions in case of occupational and dwellingrelated formaldehyde exposure. © 2012 Korean Society of Environmental Risk Assessment and Health Science and Springer.


Dou X.,Tibet Vocational Technical College | Zhang Y.,Shenzhen University | Sun N.,Shenzhen Pass Environmental Testing and Technology Co.Ltd | Wu Y.,Shenzhen University | Li L.,Shenzhen University
Cytotechnology | Year: 2014

Aqueous extract obtained from Mikania micrantha (MMAE) is commonly used as traditional medicine in some countries. We hypothesized that MMAE may inhibit tumor cell growth, both in an in vitro and in vivo setting. In in vitro experiments, two kinds of human cancer cell lines, K562 and Hela were used to test the anti-tumor activity. Inhibitory concentrations (IC50) were obtained from the inhibition curves fitted by regression analysis, inhibitory rates (%) were calculated by MTT assay, morphological changes were observed by transmission electron microscope (TEM), cell cycles were analyzed by flow cytometry (FCM), and DNA ladders were determined by agarose gel electrophoresis. The in vivo anti-tumor activity was evaluated by calculating the tumor inhibitory rates, thymus index and spleen index of S180-bearing mice. Paraffin-embedded sections were used to test the pathologic changes. The result displayed that the growth of K562 and Hela were enhanced when treated with MMAE at 20 μg/mL after 48 h. Other concentrations of MMAE (50, 100, 200, 400 μg/mL) inhibited the proliferation of both kinds of cells. The IC 50 values of K562 and Hela at 48 h were 167.16 and 196.27 μg/mL and at 72 h 98.07 and 131.56 μg/mL, respectively. The effects showed time-dose dependence. MMAE led to damages of organelles and induced apoptosis. These results were confirmed by ladder DNA fragmentation profile. MMAE also increased the percentage of cells in G2/M phase and decreased the percentage of cells undergoing G0/G1 and S phase in in vivo tests using S180 cells. MMAE showed antitummor activity in vivo, with its tumor inhibitory rate ranging from 12.1 to 46.9 %. MMAE also induced necrosis, as shown by pathological examination of Hematoxilin-Eosin stained tumor sections. Meanwhile, compared with the control group, the changes of thymus index and spleen index in MMAE treated group were not obvious. This study suggests that MMAE may be an effective agent for cancer therapy with low toxicity. © 2013 Springer Science+Business Media Dordrecht.


Xiao Y.J.,Tibet Vocational Technical College
Applied Mechanics and Materials | Year: 2014

Green Construction occupies an increasingly important position in the future construction of our country, but also the construction of an important direction of construction. Implement a, green construction and green construction management has a close relationship. I mainly green construction management problems in the analysis, put forward some suggestions and countermeasures on the basis of the hope that they carry out a comprehensive analysis of the construction works have some help. © (2014) Trans Tech Publications, Switzerland.


Xiao Y.J.,Tibet Vocational Technical College
Applied Mechanics and Materials | Year: 2013

The use of construction work performance and service life depends directly on the quality of construction and building construction technology management level. If the building construction unit is unable to ensure the improvement of the quality of construction, it is bound to waste more physical, human and financial resources, and competition in the market is always in a passive position. Quality construction has not reached the demand, the consequences will be self-evident, that is it will shorten the life of the building, slowing the progress of the construction works, construction works explicit and hidden disease prevalence. This paper analyzes the common construction quality problems in the building construction process and related technology management issues, and it proposes some practical improvements. © (2013) Trans Tech Publications, Switzerland.


Zhuoma Q.D.,Tibet Vocational technical college
Advanced Materials Research | Year: 2014

Since the first hydraulic mechanical erosion discovered in the later stage of 19th century, researchers from both abroad and home have devoted a large amount of time and energy, ranging from fluid dynamics of multiphase systems and dynamics of materials to design of impeller, processing craft and material application with multi-subject research. © (2014) Trans Tech Publications, Switzerland.

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