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Zhao B.-Y.,Northwest University, China | Liu Z.-Y.,Tibet Agriculture and Animal Husbandry College | Lu H.,Northwest University, China | Wang Z.-X.,Northwest University, China | And 6 more authors.
Agricultural Sciences in China | Year: 2010

Western grassland is the main source of living and means of production of western inhabitants. For many years, desertification and poisonous-weeds growth in grassland were resulted from over-grazing, over-reclaiming, over-spading and population growth. Western natural ecological environment is destroyed severly. Meanwhile, it has restricted the sustainable development of animal husbandry. The fast spreading poisonous-weeds, which caused grassland ecology unbalance, is one of the considerable bioecology problems and an important index of grassland degeneration. Based on analysis and induction of previous data, this article introduced the situation of poisonous-weeds disaster of western grassland in recent decades, category and distribution of poisonous-weeds, integrated control and reasonable utilization. © 2010 Chinese Academy of Agricultural Sciences.

Dorji T.,Norwegian University of Life Sciences | Dorji T.,Tibet Agriculture and Animal Husbandry College | Totland o,Norwegian University of Life Sciences | Moe S.R.,Norwegian University of Life Sciences | And 3 more authors.
Global Change Biology | Year: 2013

Global climate change is predicted to have large impacts on the phenology and reproduction of alpine plants, which will have important implications for plant demography and community interactions, trophic dynamics, ecosystem energy balance, and human livelihoods. In this article we report results of a 3-year, fully factorial experimental study exploring how warming, snow addition, and their combination affect reproductive phenology, effort, and success of four alpine plant species belonging to three different life forms in a semiarid, alpine meadow ecosystem on the central Tibetan Plateau. Our results indicate that warming and snow addition change reproductive phenology and success, but responses are not uniform across species. Moreover, traits associated with resource acquisition, such as rooting depth and life history (early vs. late flowering), mediate plant phenology, and reproductive responses to changing climatic conditions. Specifically, we found that warming delayed the reproductive phenology and decreased number of inflorescences of Kobresia pygmaea C. B. Clarke, a shallow-rooted, early-flowering plant, which may be mainly constrained by upper-soil moisture availability. Because K. pygmaea is the dominant species in the alpine meadow ecosystem, these results may have important implications for ecosystem dynamics and for pastoralists and wildlife in the region. © 2012 Blackwell Publishing Ltd.

Li K.,Huazhong Agricultural University | Li R.,Huazhong Agricultural University | Li R.,Nanjing University | Li J.,Huazhong Agricultural University | Li J.,Tibet Agriculture and Animal Husbandry College
Indian Journal of Animal Research | Year: 2016

Present study was an epidemiological survey for seroprevalence of Toxoplasma gondii (T. gondii) infection in pet dogs (n= 279) in Wuhan, Huazhong area of China, using indirect hemagglutination test (IHA). Overall seroprevalence of T. gondii infection in this area was 13.3 per cent. The prevalence in males and females was 13.4 and 13.0%, there was no significant difference (P≥0.05) between the genders. The prevalence of T. gondii infection was 12.7, 14.0, 13.3 and 12.7% in spring, summer, autumn and winter, respectively. There was a significant difference (P<0.05) in seroprevalence in age of pet dogs. Highest prevalence was found in older age as compared to young age pet dogs, With a prevelance rate of 7.6% in less than 1 year age dogs, and 16.7, 21.2 and 20.8%, between 1-3, 3-5 and 5 years and above, respectively. In different food spectrum (commercial dog food, combined dog food and homemade food), the prevalence of T. gondii infection is 11.4%, 13.7% and 14.5%, respectively. The seroprevalence in different breeds is ranged from 0 to 100.00%. We found T. gondii infection in pet dogs in Wuhan, which can adversely affect community health in this region. To the best of our knowledge, this is the first report of the prevalence of T. gondii infection in pet dogs in Wuhan. © 2016, Agricultural Research Communication Centre. All rights reserved.

Tian W.-M.,Chinese Academy of Sciences | Zhang H.,Tibet Agriculture and Animal Husbandry College | Yang S.-G.,Chinese Academy of Sciences | Shi M.-J.,Chinese Academy of Sciences | And 3 more authors.
Journal of Plant Physiology | Year: 2013

Tapping causes the loss of large amounts of latex from laticifers and subsequently enhances latex regeneration, a high carbon- and nitrogen-cost activity in rubber tree. It is suggested that a 67. kDa protein associated with protein-storing cells in the inner bark tissues of rubber tree plays an important role in meeting the nitrogen demand for latex regeneration. Here, the 67. kDa protein was further characterized by a combination of cell biological, molecular biological and biochemical techniques. Immunogold labeling showed that the 67. kDa protein was specifically localized in the central vacuole of protein-storing cells. A full-length cDNA, referred to as HbVSP1, was cloned. The HbVSP1 contained a 1584. bp open reading frame encoding a protein of 527 amino acids. The putative protein HbVSP1 shared high identity with the P66 protein from rubber tree and proteins of the linamarase, and bg1A from cassava (Manihot esculenta). HbVSP1 contained the active site sequences of β-glucosidase, TFNEP and I/VTENG. In vitro analysis showed that the 67. kDa protein exhibited the activity of both β-glucosidase and linamarase and was thus characterized as a cyanogenic β-glucosidase. Proteins immuno-related to the 67. kDa protein were present in leaves and lutoids of laticifers. Tapping down-regulated the expression of HbVSP1, but up-regulated the expression of genes encoding the key enzymes for rubber biosynthesis, while the effect of resting from tapping was the reverse. Taken together, the results suggest that the 67. kDa protein is a vacuole-localized cyanogenic β-glucosidase encoded by HbVSP1 and may have a role in nitrogen storage in inner bark tissues of trunk during the leafless periods when rubber tree is rested from tapping. © 2013 Elsevier GmbH.

Lu J.,Institute of Plateau Ecology | Lu J.,National Forest Ecosystem Observation and Research Station of Tibet Linzhi | Lan X.-Z.,Tibet Agriculture and Animal Husbandry College
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To investigate and study the endangered Tibetan medicinal plant species, their moisture content, bio-mass and resources reserves in Lhasa region. Method: The rare and endangered Tibetan medicinal plant resources were investigated by plot-quadrat method, walking and inquiry ways, sampling and drying method. Result: There were 37 species of rare and endangered plants, belonging to 22 families and 34 genera in Lhasa region. The moisture content of aerial part was higher than that of underground part in many plants. The moisture content of Przewalskia tangutica was the highest (91.97%), and the lowest one was Fritillaria delavayi (only 25.99%). The mean biomass of Rubus biflorus was the highest (1830.480 g), that of Cordyceps sinensis was the lowest (0.291 g). The root-shoot ratio of Asparagus filicinus was the maximum (5.313), the minimum was Aconitum gymnandrum (0.286). The largest output was 18.000 kg · hm-2 for Berberis agricola, the output of Saxifraga pasumensis was the lowest (0.007 kg · hm-2). The resources reserves of the rare and endangered plants were 15683.697 t in Lhasa region, the maximum was 7690.230 t for B. agricola, 49.03% of the total reserves, the minimum was 2.393 t for S. pasumensis, only 0.015%. Conclusion: The characteristics of rare and endangered plants were as follows: abundant species and complex habitats, widely distribution but uneven, rich reserves and high economic value. We suggested to update the endangered level of medicinal plants, strengthen the scientific research on these plants, maintain sustainable utilization of the rare and endangered plants in Lhasa region.

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