Tibet Agriculture and Animal Husbandry College
Tibet Agriculture and Animal Husbandry College
Chen S.,Henan University of Science and Technology |
Chen S.,Tibet Agriculture and Animal Husbandry College |
Zhao H.,Henan University of Science and Technology |
Wang M.,Henan University of Science and Technology |
And 5 more authors.
Frontiers in Plant Science | Year: 2017
Ubiquitination is a common regulatory mechanism, playing a critical role in diverse cellular and developmental processes in eukaryotes. However, a few reports on the functional correlation between E3 ubiquitin ligases and reactive oxygen species (ROS) or reactive nitrogen species (RNS) metabolism in response to stress are currently available in plants. In the present study, the E3 ubiquitin ligase gene AdBiL (Adi3 Binding E3 Ligase) was introduced into tomato line Ailsa Craig via Agrobacterium-mediated method. Transgenic lines were confirmed for integration into the tomato genome using PCR. Transcription of AdBiL in various transgenic lines was determined using real-time PCR. Evaluation of stress tolerance showed that T1 generation of transgenic tomato lines showed only mild symptoms of chilling injury as evident by higher biomass accumulation and chlorophyll content than those of non-transformed plants. Compared with wild-type plants, the contents of AsA, AsA/DHA, GSH and the activity of GaILDH, γ-GCS and GSNOR were increased, while H2 O2, O.− 2, MDA, NO, SNOs, and GSNO accumulations were significantly decreased in AdBiL overexpressing plants in response to chilling stress. Furthermore, transgenic tomato plants overexpressing AdBiL showed higher activities of enzymes such as G6PDH, 6PGDH, NADP-ICDH, and NADP-ME involved in pentose phosphate pathway (PPP). The transgenic tomato plants also exhibited an enhanced tolerance against the necrotrophic fungus Cladosporium fulvum. Tyrosine nitration protein was activated in the plants infected with leaf mold disease, while the inhibition could be recovered in AdBiL gene overexpressing lines. Taken together, our results revealed a possible physiological role of AdBiL in the activation of the key enzymes of AsA–GSH cycle, PPP and down-regulation of GSNO reductase, thereby reducing oxidative and nitrosative stress in plants. This study demonstrates an optimized transgenic strategy using AdBiL gene for crop improvement against biotic and abiotic stress factors. © 2017 Chen, Zhao, Wang, Li, Wang, Wang, Liu and Ahammed.
Wei X.-H.,Tibet Agriculture and Animal Husbandry College |
Jin L.,Tibet Peoples Hospital in Linzhi
Chinese Pharmacological Bulletin | Year: 2010
Oxytropis falcata is a perennial acaulescent herb of Oxytropis leguminosae. It is an impointan traditional tibetan medicinal material of high pharmaceutical value, which grows widely in Tibet. In recent years, there has been an extensive study of this plant. This article is to summarize the knowledge of Oxytropis flacanta: its chemical composition, pharmacological and clinical application, and recommendation for furthur research.
Zong Y.,Tibet Agriculture and Animal Husbandry College |
Bai Q.,Tibet Agriculture and Animal Husbandry College |
Chen X.,Tibet Agriculture and Animal Husbandry College
International Journal of Earth Sciences and Engineering | Year: 2016
In this paper, Correlation analysis is used to analyze Fractal dimension to valuation of eutrophication. The results are showed that correlation dimension of five index with the corresponding index are the same direction. In the fact, correlation dimension of four indexes with the corresponding index are the same direction and correlation dimension of one index with the corresponding index is the different direction. The diversification of the different direction will cause an adverse change in the evaluation results and is the opposite of the same direction. So we provide a method for pre-processing of data that is normalization method and conducted a correlation analysis and verification of accuracy. The results are showed that correlation dimension of five indexes with the corresponding index are the same direction, and that the correlation between correlation dimension and the corresponding index is very high, and that the accuracy of evaluation results is very high. © 2016 CAFET-INNOVA TECHNICAL SOCIETY. All rights reserved.
Dorji T.,Norwegian University of Life Sciences |
Dorji T.,Tibet Agriculture and Animal Husbandry College |
Totland o,Norwegian University of Life Sciences |
Moe S.R.,Norwegian University of Life Sciences |
And 3 more authors.
Global Change Biology | Year: 2013
Global climate change is predicted to have large impacts on the phenology and reproduction of alpine plants, which will have important implications for plant demography and community interactions, trophic dynamics, ecosystem energy balance, and human livelihoods. In this article we report results of a 3-year, fully factorial experimental study exploring how warming, snow addition, and their combination affect reproductive phenology, effort, and success of four alpine plant species belonging to three different life forms in a semiarid, alpine meadow ecosystem on the central Tibetan Plateau. Our results indicate that warming and snow addition change reproductive phenology and success, but responses are not uniform across species. Moreover, traits associated with resource acquisition, such as rooting depth and life history (early vs. late flowering), mediate plant phenology, and reproductive responses to changing climatic conditions. Specifically, we found that warming delayed the reproductive phenology and decreased number of inflorescences of Kobresia pygmaea C. B. Clarke, a shallow-rooted, early-flowering plant, which may be mainly constrained by upper-soil moisture availability. Because K. pygmaea is the dominant species in the alpine meadow ecosystem, these results may have important implications for ecosystem dynamics and for pastoralists and wildlife in the region. © 2012 Blackwell Publishing Ltd.
Zhang Z.-Y.,Tianjin University |
Zhang Z.-Y.,Tibet Agriculture and Animal Husbandry College |
Zhang Y.-P.,Tianjin University
Proceedings of the 2012 International Conference on Industrial Control and Electronics Engineering, ICICEE 2012 | Year: 2012
Mining frequent item sets is an important issue in association rules community. This paper proposes a parallel algorithm for mining frequent item sets based on bit matrix. The algorithm reduces the memory space and the I/O overhead, for it scans database only once and builds a compressed bit matrix. It combines both top-down approach and bottom-up approach to improve the efficiency of pruning, and uses dynamic scheduling parallel multi-threaded of OpenMP to mine frequent item sets. The experiments show that this algorithm has higher computing efficiency than Apriori algorithm. © 2012 IEEE.
Dorji T.,Norwegian University of Life Sciences |
Totland O.,Tibet Agriculture and Animal Husbandry College |
Moe S.R.,Tibet Agriculture and Animal Husbandry College
Rangeland Ecology and Management | Year: 2013
Grazing is regarded as one of the most important factors influencing rangeland ecosystems. Many previous studies have used indirect measures (proxies) of grazing intensity, such as the distance from congregation areas and the number of herbivore excreta, to estimate effects of grazing on vegetation parameters in rangeland ecosystems. However, little is known about how such proxies reflect the true grazing intensity, and if their suitability is consistent across landscape conditions. We measured grazing intensity based on the amount of plant ramets being grazed in 75 plots positioned across a rangeland landscape in central Tibet. Several grazing intensity proxies (distance from pastoral camp sites, number of yak dung patches (old and fresh), sheep and goat dropping piles, and pika (Ochotona curzoniae) burrows (active and abandoned)) were also measured in each plot. Regression models were used to examine the suitability of these proxies in predicting our measure of real grazing intensity. None of the examined proxies satisfactorily predicted real grazing intensity in our study system. The distance from camp sites was the best proxy for grazing intensity (P<0.001), but it explained only 30% of its variation. Fresh yak dung and active pika burrows explained only 10% and 3% of the variation in grazing intensity, respectively. The suitability of using proxies for grazing intensity did not significantly differ along landscape parameters (elevation, aspect directions, and slope angle) (P>0.05), except that the number of animal excreta and pika burrows functioned better as proxies at low, compared to high, elevation (P<0.05). Our results should be of concern since many studies use proxies to estimate effects of grazing on vegetation dynamics and ecosystem properties. We recommend awareness of potential limitations and suitability of proxies, when real grazing intensity is not measured. © 2013 The Society for Range Management.
Li K.,Huazhong Agricultural University |
Li R.,Huazhong Agricultural University |
Li R.,Nanjing University |
Li J.,Huazhong Agricultural University |
Li J.,Tibet Agriculture and Animal Husbandry College
Indian Journal of Animal Research | Year: 2016
Present study was an epidemiological survey for seroprevalence of Toxoplasma gondii (T. gondii) infection in pet dogs (n= 279) in Wuhan, Huazhong area of China, using indirect hemagglutination test (IHA). Overall seroprevalence of T. gondii infection in this area was 13.3 per cent. The prevalence in males and females was 13.4 and 13.0%, there was no significant difference (P≥0.05) between the genders. The prevalence of T. gondii infection was 12.7, 14.0, 13.3 and 12.7% in spring, summer, autumn and winter, respectively. There was a significant difference (P<0.05) in seroprevalence in age of pet dogs. Highest prevalence was found in older age as compared to young age pet dogs, With a prevelance rate of 7.6% in less than 1 year age dogs, and 16.7, 21.2 and 20.8%, between 1-3, 3-5 and 5 years and above, respectively. In different food spectrum (commercial dog food, combined dog food and homemade food), the prevalence of T. gondii infection is 11.4%, 13.7% and 14.5%, respectively. The seroprevalence in different breeds is ranged from 0 to 100.00%. We found T. gondii infection in pet dogs in Wuhan, which can adversely affect community health in this region. To the best of our knowledge, this is the first report of the prevalence of T. gondii infection in pet dogs in Wuhan. © 2016, Agricultural Research Communication Centre. All rights reserved.
Lu J.,Institute of Plateau Ecology |
Lu J.,National Forest Ecosystem Observation and Research Station of Tibet Linzhi |
Lan X.-Z.,Tibet Agriculture and Animal Husbandry College
Zhongguo Zhongyao Zazhi | Year: 2013
Objective: To investigate and study the endangered Tibetan medicinal plant species, their moisture content, bio-mass and resources reserves in Lhasa region. Method: The rare and endangered Tibetan medicinal plant resources were investigated by plot-quadrat method, walking and inquiry ways, sampling and drying method. Result: There were 37 species of rare and endangered plants, belonging to 22 families and 34 genera in Lhasa region. The moisture content of aerial part was higher than that of underground part in many plants. The moisture content of Przewalskia tangutica was the highest (91.97%), and the lowest one was Fritillaria delavayi (only 25.99%). The mean biomass of Rubus biflorus was the highest (1830.480 g), that of Cordyceps sinensis was the lowest (0.291 g). The root-shoot ratio of Asparagus filicinus was the maximum (5.313), the minimum was Aconitum gymnandrum (0.286). The largest output was 18.000 kg · hm-2 for Berberis agricola, the output of Saxifraga pasumensis was the lowest (0.007 kg · hm-2). The resources reserves of the rare and endangered plants were 15683.697 t in Lhasa region, the maximum was 7690.230 t for B. agricola, 49.03% of the total reserves, the minimum was 2.393 t for S. pasumensis, only 0.015%. Conclusion: The characteristics of rare and endangered plants were as follows: abundant species and complex habitats, widely distribution but uneven, rich reserves and high economic value. We suggested to update the endangered level of medicinal plants, strengthen the scientific research on these plants, maintain sustainable utilization of the rare and endangered plants in Lhasa region.
Tian W.-M.,Chinese Academy of Sciences |
Zhang H.,Tibet Agriculture and Animal Husbandry College |
Yang S.-G.,Chinese Academy of Sciences |
Shi M.-J.,Chinese Academy of Sciences |
And 3 more authors.
Journal of Plant Physiology | Year: 2013
Tapping causes the loss of large amounts of latex from laticifers and subsequently enhances latex regeneration, a high carbon- and nitrogen-cost activity in rubber tree. It is suggested that a 67. kDa protein associated with protein-storing cells in the inner bark tissues of rubber tree plays an important role in meeting the nitrogen demand for latex regeneration. Here, the 67. kDa protein was further characterized by a combination of cell biological, molecular biological and biochemical techniques. Immunogold labeling showed that the 67. kDa protein was specifically localized in the central vacuole of protein-storing cells. A full-length cDNA, referred to as HbVSP1, was cloned. The HbVSP1 contained a 1584. bp open reading frame encoding a protein of 527 amino acids. The putative protein HbVSP1 shared high identity with the P66 protein from rubber tree and proteins of the linamarase, and bg1A from cassava (Manihot esculenta). HbVSP1 contained the active site sequences of β-glucosidase, TFNEP and I/VTENG. In vitro analysis showed that the 67. kDa protein exhibited the activity of both β-glucosidase and linamarase and was thus characterized as a cyanogenic β-glucosidase. Proteins immuno-related to the 67. kDa protein were present in leaves and lutoids of laticifers. Tapping down-regulated the expression of HbVSP1, but up-regulated the expression of genes encoding the key enzymes for rubber biosynthesis, while the effect of resting from tapping was the reverse. Taken together, the results suggest that the 67. kDa protein is a vacuole-localized cyanogenic β-glucosidase encoded by HbVSP1 and may have a role in nitrogen storage in inner bark tissues of trunk during the leafless periods when rubber tree is rested from tapping. © 2013 Elsevier GmbH.
Ma H.-P.,Research Institute of Plateau Ecology |
Yang X.-L.,Tibet Agriculture and Animal Husbandry College |
Guo Q.-Q.,Research Institute of Plateau Ecology |
Zhang X.-J.,Research Institute of Plateau Ecology |
Zhou C.-N.,Research Institute of Plateau Ecology
Journal of Mountain Science | Year: 2016
Labile organic carbon (LOC) is one of the most important indicators of soil organic matter quality and dynamics elevation and plays important function in the Tibetan Plateau climate. However, it is unknown what the sources and causes of LOC contamination are. In this study, soil organic carbon (SOC), total nitrogen (TN), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) and LOC were analyzed based on different soil horizons and elevations using turnover time in an experimental site (3700 m to 4300 m area) in Sygera. SOC and LOC in higher-elevation vegetation types were higher than that of in lower-elevation vegetation types. Our results presented that the soil microbial biomass carbon (SMBC) and soil microbial biomass nitrogen (SMBN) were positively correlated with SOC. The content of easily oxidized carbon (EOC), particulate organic carbon (POC) and light fraction organic carbon (LFOC) decreased with depth increasing and the content were the lowest in the 60 cm to 100 cm depth. The total SOC, ROC and POC contents decreased with increasing soil horizons. The SOC, TN, MBC and MBN contents increased with increasing altitude in the Sygera Mountains. The MBC and MBN contents were different with the changes of SOC (p<0.05), meanwhile, both LFOC and POC were related to total SOC (p<0.05). The physical and chemical properties of soil, including temperature, humidity, and altitude, were involved in the regulation of SOC, TN, MBC, MBN and LFOC contents in the Sygera Mountains, Tibetan Plateau. © 2016, Science Press, Institute of Mountain Hazards and Environment, CAS and Springer-Verlag Berlin Heidelberg.