Tibet Academy of Agriculture and Animal science

Lhasa, China

Tibet Academy of Agriculture and Animal science

Lhasa, China
SEARCH FILTERS
Time filter
Source Type

Liang J.,CAS Chengdu Institute of Biology | Liang J.,University of Chinese Academy of Sciences | Deng G.,CAS Chengdu Institute of Biology | Long H.,CAS Chengdu Institute of Biology | And 9 more authors.
Molecular Breeding | Year: 2012

Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley. © 2011 Springer Science+Business Media B.V.


Wang C.-P.,CAS Chengdu Institute of Biology | Wang C.-P.,University of Chinese Academy of Sciences | Pan Z.-F.,CAS Chengdu Institute of Biology | Nima Z.-X.,Tibet Academy of Agriculture and Animal science | And 8 more authors.
Journal of the Science of Food and Agriculture | Year: 2011

Background: The starch granule-associated proteins (SGAPs) are the minor components of the starch granules and a majority of them are believed to be starch biosynthetic enzymes. The Qinghai-Tibet Plateau in China, one of the centres of origin of cultivated barley, is abundant in hull-less barley resources which exhibit high polymorphism in SGAPs. Results: The SGAPs of hull-less barley from Qinghai-Tibet Plateau were analysed by one-dimensional (1-D) SDS-PAGE, 2-D PAGE and ESI-Q-TOF MS/MS. In the 1-D SDS-PAGE gel, four proteins including a 80 kDa starch synthase, actin, actin 4 and ATP synthase β-subunit were identified as novel SGAPs. A total of six different bands were identified as starch granule-bound starch synthase I (GBSSI) and the segregation of the novel GBSSI bands in F1 and F2 seeds derived from yf127 × yf70 was in accordance with Mendel's law. In the 2-D PAGE gel, 92 spots were identified as 42 protein species which could be classified into 15 functional groups. Thirteen protein species were identified as SGAPs for the first time and multiple spots were identified as GBSSI. Conclusion: This study revealed novel SGAPs in hull-less barley from the Qinghai-Tibet Plateau in China and these will be significant in further studies of starch biosynthesis in barley. Copyright © 2011 Society of Chemical Industry.


Qiu J.,CAS Institute of Botany | Qiu J.,University of Chinese Academy of Sciences | Xue X.,Chinese Academy of Agricultural Sciences | Chen F.,CAS Institute of Botany | And 8 more authors.
Plant Cell Reports | Year: 2010

Snow lotus is commonly used as a medicinal plant and has great pharmacological value. To protect these endangered plants, in vitro propagation and cell cultures have been established in order to meet the growing market demand. The phenolic composition, antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC) from three most commonly used species, in vitro propagated lines and the cell cultures were investigated to qualify their pharmacological value. Quantitative analysis showed that the phenolics varied greatly among different species and the same species at different habitats. From this it can be inferred that the phenolics were influenced by genetic background and the environmental conditions. Significant correlations were observed between the antioxidant activity and several phenolics/TPC/TFC, suggesting that the phenolics are a major contributor of the antioxidant activity and are important for quality evaluation of snow lotus. Based on the abundance of phenolics, TPC, TFC and antioxidant activity, the order of the quality for wild species would be Saussurea involucrata > Saussurea medusa > Saussurea gossypiphora. For S. medusa, its quality judged by origin would be Shigatse > Lhasa > Nagqu. For in vitro propagated plants, the matured plants could be a reliable substitute for wild plants, and the dynamics of phenolics is critical for quality control of this monocarpic species. We provide the first report of quality comparison between the wild plants and the cell cultures. The advantages of developing cell cultures as alternatives for plants collected from the wild are discussed. © 2010 Springer-Verlag.


Yu S.,CAS Chengdu Institute of Biology | Yu S.,University of Chinese Academy of Sciences | Long H.,CAS Chengdu Institute of Biology | Deng G.,CAS Chengdu Institute of Biology | And 6 more authors.
Plant Molecular Biology Reporter | Year: 2015

Whether the caryopsis of barley is naked or covered is controlled by the Nud gene encoding an ethylene response factor (ERF) family transcription factor (TF) of the AP2/ERF TF superfamily that is involved in the lipid biosynthesis pathway. In covered barley, lipids on the surface of caryopses act as a glue for their tight adhesion with hulls. Separation of hulls in naked barley is due to the absence of surface lipids on caryopses. A natural deletion event of Nud was found to be the determinant of naked caryopsis and had been proposed as the monophyletic origin of domesticated naked barley. In the present study, we re-sequenced the nud locus in 162 barleys, comprising 79 covered and 83 naked barleys varieties. Besides the normal deletion mutation of Nud contributing to naked caryopsis, we found a novel allele of Nud, designated as nud1.g, distinctly in three naked barleys collected from Tibet. nud1.g contains a non-synonymous SNP T643A when compared with the functional Nud gene. Genetic analysis indicated that the SNP T643A of nud1.g co-segregates with the naked phenotype. The nud1.g gene was expressed normally in the three naked barleys. Furthermore, in silico prediction of functionally conserved sites and 3D structures showed that the amino acid substitution (valine to aspartate) caused by SNP T643A may lead to a dramatic structural alteration of NUD that may result in loss of function. This study provides evidence of a new origin of the naked phenotype of domesticated barley in Tibet. © 2015 Springer Science+Business Media New York


Chen W.Y.,CAS Chengdu Institute of Biology | Chen W.Y.,China West Normal University | Liu Z.M.,CAS Chengdu Institute of Biology | Liu Z.M.,China West Normal University | And 8 more authors.
Genetics and Molecular Research | Year: 2014

Lodging (LD) is a major constraint limiting the yield and forage quality of barley. Detailed analyses of LD component (LDC) traits were conducted using 246 F2 plants generated from a cross between cultivars ZQ320 and 1277. Genetic relationships between LD and LDC were evaluated by unconditional and conditional quantitative trait locus (QTL) mapping with 117 simple sequence repeat markers. Ultimately, 53 unconditional QTL related to LD were identified on seven barley chromosomes. Up to 15 QTL accounted for over 10% of the phenotypic variation, and up to 20 QTL for culm strength were detected. Six QTL with pleiotropic effects showing significant negative correlations with LD were found between markers Bmag353 and GBM1482 on chromosome 4H. These alleles and alleles of QTL for wall thickness, culm strength, plant height, and plant weight originated from ZQ320. Conditional mapping identified 96 additional QTL for LD. Conditional QTL analysis demonstrated that plant height, plant height center of gravity, and length of the sixth internode had the greatest contribution to LD, whereas culm strength and length of the fourth internode, and culm strength of the second internode were the key factors for LD-resistant. Therefore, lodging resistance in barley can be improved based on selection of alleles affecting culm strength, wall thickness, plant height, and plant weight. The conditional QTL mapping method can be used to evaluate possible genetic relationships between LD and LDC while efficiently and precisely determining counteracting QTL, which will help in understanding the genetic basis of LD in barley.© FUNPEC-RP www.funpecrp.com.br.


Wang K.,CAS Institute of Microbiology | Wang K.,University of Chinese Academy of Sciences | Bao L.,CAS Institute of Microbiology | Xiong W.,Tibet Academy of Agriculture and Animal Science | And 5 more authors.
Journal of Natural Products | Year: 2015

(Chemical Equqation Presented). Sixteen new lanostane triterpenes, ganoleucoins A-P (1-16), together with 10 known tripterpenes (17-26), were isolated from the cultivated fruiting bodies of Ganoderma leucocontextum, a new member of the Ganoderma lucidum complex. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical transformation. The inhibitory effects of 1-26 on HMG-CoA reductase and α-glucosidase were tested in vitro. Compounds 1, 3, 6, 10-14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity against HMG-CoA reductase than the positive control atorvastatin. Compounds 13, 14, and 16 presented potent inhibitory activity against α-glucosidase from yeast with IC50 values of 13.6, 2.5, and 5.9 μM, respectively. In addition, the cytotoxicity of 1-26 was evaluated against the K562 and PC-3 cell lines by the MTT assay. Compounds 1, 2, 6, 7, 10, 12, 16, 18, and 25 exhibited cytotoxicity against K562 cells with IC50 values in the range 10-20 μM. Paclitaxel was used as the positive control with an IC50 value of 0.9 μM. This is the first report of secondary metabolites from this medicinal mushroom. © 2015 The American Chemical Society and American Society of Pharmacognosy.


Waldron S.,University of Queensland | Pubuzhuoma,Tibetan Academy of Agriculture and Animal Husbandry science | Brown C.,University of Queensland | Wujincuomu,Tibetan Academy of Agriculture and Animal Husbandry science | Jin T.,Tibet Academy of Agriculture and Animal science
Chemical and Biochemical Engineering Quarterly | Year: 2016

With small land sizes, precarious food balances, and a changing institutional landscape, farmers in Central Tibet have had to be resilient and adaptive in their livelihood strategies. Rural Tibetans retain a base in semi-subsistence agriculture from which to pursue other major opportunities that have arisen in the 2000s, including off-farm work and caterpillar fungus collection. As reported in this paper, farmers have been given a further boost in recent years through buoyant food markets, and after decades of neglect, increased policy attention to agriculture. This has increased household, wealth and reduced vulnerability, but with very low surpluses has had a limited effect on cash income, the vast majority of which must still be sourced off-farm. Thus, semi-subsistence agriculture provides a 'pathway out of poverty' including into the non-farm sector, but the transition will not be linear and will be influenced by a complex combination of forces. The paper documents the way that these forces have played out at the household level in the case study township of Duopozhang in Shannan Prefecture between 2010 and 2015. Analysis is based on an agricultural-economicbiophysical household model populated by detailed household surveys, and contextualized and cross-verified with detailed primary and secondary data at township up to autonomous region levels. This may shed light on recent developments in agricultural areas of Central Tibet that are not easily accessible or widely reported.


Waldron S.,University of Queensland | Pubuzhuoma,Tibetan Academy of Agriculture and Animal Husbandry science | Brown C.,University of Queensland | Wujincuomu,Tibetan Academy of Agriculture and Animal Husbandry science | And 2 more authors.
Himalaya | Year: 2015

With small land sizes, precarious food balances, and a changing institutional landscape, farmers in Central Tibet have had to be resilient and adaptive in their livelihood strategies. Rural Tibetans retain a base in semi-subsistence agriculture from which to pursue other major opportunities that have arisen in the 2000s, including off-farm work and caterpillar fungus collection. As reported in this paper, farmers have been given a further boost in recent years through buoyant food markets, and after decades of neglect, increased policy attention to agriculture. This has increased household wealth and reduced vulnerability, but with very low surpluses has had a limited effect on cash income, the vast majority of which must still be sourced off-farm. Thus, semi-subsistence agriculture provides a 'pathway out of poverty' including into the non-farm sector, but the transition will not be linear and will be influenced by a complex combination of forces. The paper documents the way that these forces have played out at the household level in the case study township of Duopozhang in Shannan Prefecture between 2010 and 2015. Analysis is based on an agricultural-economicbiophysical household model populated by detailed household surveys, and contextualized and cross-verified with detailed primary and secondary data at township up to autonomous region levels. This may shed light on recent developments in agricultural areas of Central Tibet that are not easily accessible or widely reported.


PubMed | Tibet Academy of Agriculture and Animal Science and Chinese Academy of Agricultural Sciences
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2014

This study investigated geographic and pairwise distances among seven Chinese local and four introduced sheep populations via analysis of 26 microsatellite DNA markers. Genetic polymorphism was rich, and the following was discovered: 348 alleles in total were detected, the average allele number was 13.38, the polymorphism information content (PIC) of loci ranged from 0.717 to 0.788, the number of effective alleles ranged from 7.046 to 7.489, and the observed heterozygosity ranged from 0.700 to 0.768 for the practical sample, and from 0.712 to 0.794 for expected heterozygosity. The Wrights F-statistic of subpopulations within the total (FST) was 0.128, the genetic differentiation coefficient (GST) was 0.115, and the average gene flow (Nm) was 1.703. The phylogenetic trees based on the neighbor-joining method by Neis genetic distance (DA) and Neis standard genetic distance (DS) were similar. Sheep populations clustered into group 1 (Ta, M, L, H, O, G, and Q breeds) and group 2 (PD, WS, B, and T breeds). These results will have an important value applied and directive significance for sheep breeding in the future.


PubMed | Tibet Academy of Agriculture and Animal Science and CAS Institute of Microbiology
Type: Journal Article | Journal: Journal of natural products | Year: 2015

Sixteen new lanostane triterpenes, ganoleucoins A-P (1-16), together with 10 known tripterpenes (17-26), were isolated from the cultivated fruiting bodies of Ganoderma leucocontextum, a new member of the Ganoderma lucidum complex. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical transformation. The inhibitory effects of 1-26 on HMG-CoA reductase and -glucosidase were tested in vitro. Compounds 1, 3, 6, 10-14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity against HMG-CoA reductase than the positive control atorvastatin. Compounds 13, 14, and 16 presented potent inhibitory activity against -glucosidase from yeast with IC values of 13.6, 2.5, and 5.9 M, respectively. In addition, the cytotoxicity of 1-26 was evaluated against the K562 and PC-3 cell lines by the MTT assay. Compounds 1, 2, 6, 7, 10, 12, 16, 18, and 25 exhibited cytotoxicity against K562 cells with IC values in the range 10-20 M. Paclitaxel was used as the positive control with an IC value of 0.9 M. This is the first report of secondary metabolites from this medicinal mushroom.

Loading Tibet Academy of Agriculture and Animal science collaborators
Loading Tibet Academy of Agriculture and Animal science collaborators