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Yang J.,Tianjin Medical University | Wang L.-L.,Tianjin Institute of Pharmaceutical Research | Zhou X.-T.,Tianjin University of Traditional Chinese Medicine | Yuan J.-Z.,Shenyang Pharmaceutical University | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2014

Objective To study the chemical constituents of Melilotus officinalis. Methods The compounds were isolated and purified by silica gel column chromatography, gel chromatography, and ODS column chromatography. Their structures were elucidated by chemical and spectral analyses. Results Twelve compounds were obtained from the 70% ethanol extract of M officinalis and their structures were identified as β-sitosterol (1), coumarin (2), methyl 3-(4-hydroxyphenyl)propionate (3), kaempferol-3-O-α-L-rhamnoside (4), rutin (5), apigenin-7-O-β-D-lutinoside (6), dibutyl phthalate (7), robinin (8) clovin (9), kaempferol-3-O-rutinoside (10), isoquerecitrin (11), and medicarpin 3-O-β-glucopyranoside (12). Conclusion Compounds 3, 4, 6, 7, 11, and 12 are isolated from plants from Melilotus Miller for the first time.

Wang L.,Tianjin University | Wang L.,Tianjin Zhongxin Pharmaceutical Group Co. | Zhang J.-Z.,Tianjin University | Liu Z.,Tianjin University | Gao W.-Y.,Tianjin University
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To study the active constituents in the methanol extract of Weichang'an Pill (MEWP) and their anti-inflammatory activity. Methods: The HPLC method was used to anylyze the constituents in MEWP. The anti-inflammatory effects of MEWP were observed on the acute anti-inflammatory models of xylene-induced ear edema, carrageenan-induced paw edema, and acetic acid-induced vascular permeability; Meanwhile the chronic anti-inflammatory model of granuloma was induced by cotton pellet implantation. Results: Twelve active constituents in MEWP were analyzed, including costunolide and dehydro-α-curcumene from Aucklandiae Radix; naringin, hesperedin, and neohesperedin from Aurantii Fructus; magnolol and honokiol from Magnoliae Officinalis Cortex; aloe-emodin, rhein, emodin, chrysophanol, and physcion from Rhei. Radix et Rhizoma. At the dose of 800 mg/kg, the MEWP showed the inhibitory rate of 39.46% on the ear edema and 35.37% on the increasing of capillary permeability induced by acetic acid in mice. It could decrease the paw edema caused by carrageenan after 1 h and inhibit the granuloma induced by cotton pellet implantation. Conclusion: The MEWP could antagonize the reaction of inflammation.

Tianjin Zhongxin Pharmaceutical Group Corporation Ltd., Tianjin Traditional Chinese Medicine Manufactory and Sos United States Company | Date: 2003-10-28


Chen Y.-L.,Tianjin University of Traditional Chinese Medicine | Wang S.-P.,Chinese Peoples Armed Police forces Academy | Wang L.,Tianjin Zhongxin Pharmaceutical Group Co. | Jinn Z.-X.,Tianjin Zhongxin Pharmaceutical Group Co. | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016

To conduct multiple-reaction monitoring ( MRM) quantitative analysis with high-performance liquid chromatography coupled with mass spectrometry method , establish the quantification method of magnolol and honokiol in blood sample under negative ion mode with ibuprofen as internal standard , investigate the pharmacokinetic process of lignans constituents after oral administration of Weichang'an piil(WCA) at different doses , ad provide theoretical basis to further reveal the material basis of WCA's anti-diarrhea effect. In the plasma samples , the linear relationship was good over the concentration range of 5. 25 to 1 344. 00 |ig • L 1 for magnolol and 10. 08 to 2 580. 00 |ig • L 1 for honokiol. The results of precision , stability , and extraction recovery tests showed that the determination method of plasma concentration for such compositions was stable and reliable. Dose-dependence was shown for magnolol and honokiol in the plasma concentration-time profile. The results indicated that the time to reach the maximum plasma concentration ( 7max) for lignanoids was 0. 55-1. 42 h, when the maximum plasma concentration( cmax) could reach 996. 36-2 330. 96,189. 87-1 469. 43 |ig • L 1 respectively for magnolol and honokiol. The lignanoids could be absorbed rapidly in the blood after oral administration of WAC piils, providing experimental basis to prove rapid and long-acting anti-diarrhea effect of WAC piils after oral administration.,.

Chen Y.,Tianjin University of Traditional Chinese Medicine | Zheng H.,Tianjin University | Zhang J.,Tianjin University | Wang L.,Tianjin Zhongxin Pharmaceutical Group Co. | And 2 more authors.
RSC Advances | Year: 2016

The aim of the study was to investigate the protective effects of costunolide (Co) and dehydrocostus (De) in 5-fluorouracil (5-FU)-induced intestinal mucositis (IM) as well as the potential mechanisms involved. Male Kunming mice were given 5-FU (60 mg kg-1 per day) by intraperitoneal injections for 5 consecutive days and IM was evaluated histochemically. Co (5, 20 mg kg-1) and De (5, 20 mg kg-1) were orally administered once daily for 8 days. Repeated 5-FU treatment caused severe IM including morphological damage, which was accompanied by feeding reduction, body weight loss and diarrhea. Daily intragastric administration of Co or De significantly relieved the severity of IM through promoting intestinal mucosal recovery, inhibiting reactive oxygen species and ameliorating the inflammatory responses. Accordingly, Co and De may be promising therapeutic candidates and clinically used for the prevention of IM during cancer chemotherapy. © 2016 The Royal Society of Chemistry.

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