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Chen Y.-L.,Tianjin University of Traditional Chinese Medicine | Wang S.-P.,Chinese People's Armed Police forces Academy | Wang L.,Tianjin Zhongxin Pharmaceutical Group Co. | Jinn Z.-X.,Tianjin Zhongxin Pharmaceutical Group Co. | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016

To conduct multiple-reaction monitoring ( MRM) quantitative analysis with high-performance liquid chromatography coupled with mass spectrometry method , establish the quantification method of magnolol and honokiol in blood sample under negative ion mode with ibuprofen as internal standard , investigate the pharmacokinetic process of lignans constituents after oral administration of Weichang'an piil(WCA) at different doses , ad provide theoretical basis to further reveal the material basis of WCA's anti-diarrhea effect. In the plasma samples , the linear relationship was good over the concentration range of 5. 25 to 1 344. 00 |ig • L 1 for magnolol and 10. 08 to 2 580. 00 |ig • L 1 for honokiol. The results of precision , stability , and extraction recovery tests showed that the determination method of plasma concentration for such compositions was stable and reliable. Dose-dependence was shown for magnolol and honokiol in the plasma concentration-time profile. The results indicated that the time to reach the maximum plasma concentration ( 7max) for lignanoids was 0. 55-1. 42 h, when the maximum plasma concentration( cmax) could reach 996. 36-2 330. 96,189. 87-1 469. 43 |ig • L 1 respectively for magnolol and honokiol. The lignanoids could be absorbed rapidly in the blood after oral administration of WAC piils, providing experimental basis to prove rapid and long-acting anti-diarrhea effect of WAC piils after oral administration.,.


Liu B.,Tianjin Medical University | Liu T.,Tianjin Zhongxin Pharmaceutical Group Corporation LTD. | Wang X.,Tianjin Medical University | Zheng X.,Tianjin Zhongxin Pharmaceutical Group Corporation LTD. | And 2 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2016

Guchang capsule (GC) is a Chinese materia medica standardized product extracted from 15 Chinese traditional medical herbs and it has been clinically used in the treatment of intestinal disease. In this study, in order to extend the research of GC in intestinal disease, we were aiming to evaluate potential effects of GC on dextran sulphate sodium- (DSS-) induced murine experimental colitis and to elucidate the underlying mechanisms. GC treatment attenuated DSS-induced body weight loss and reduced the mortality. Moreover, GC treatment prevented DSS-induced colonic pathological damage; meanwhile it inhibited proinflammatory cytokines production in colon tissues. In vitro, GC significantly reduced LPS-induced proinflammatory cytokines production via inhibiting the activation of NF-κB in macrophage cells, and the expressions of several long noncoding RNAs (lncRNAs) which were reported in regulating NF-κB signaling pathway were obviously affected by adding GC into culture medium. In conclusion, our data suggested that administration of GC exhibits therapeutic effects on DSS-induced colitis partially through regulating the expression of NF-κB related lncRNAs in infiltrating immune cells. © 2016 Baoshan Liu et al.


Yan S.-C.,Tianjin Medical University | Yin Y.-Z.,Tianjin Zhongxin Pharmaceutical Group Co. | Ai L.,Tianjin Zhongxin Pharmaceutical Group Co. | Nie X.-J.,Tianjin Zhongxin Pharmaceutical Group Co. | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2016

Objective: To investigate the clinical efficacy of Wei Chang An Pills combined with Artemether Injection in the treatment of malaria (gastrointestinal type). Methods: Patients (253 cases) with malaria (gastrointestinal type) in Hospital de L'amitie Sino-Congolaise in The Republic of Congo from July 2013 to December 2014 were randomly divided into control and treatment groups. Patients (130 cases) in the treatment group were im administered with Artemether Injection and po administered with Wei Chang An Pills. Patients (123 cases) in the control group were im administered with Artemether Injection and po administered with Vitamin B6 Tablets. Patients in both two groups were treated for 7 d. After the treatment, the efficacy was evaluated, and the improvement of nausea, vomiting, abdominal pain, and diarrhea was observed, white blood cells and red blood cells in feces were simultaneously detected, numbers of plasmodium in peripheral blood were counted. Results: After the treatment for 5 d, the clinical symptoms of all the patients (130 cases) in the treatment group disappeared. After the treatment for 7 d, clinical symptoms of patients (87 cases) in the control group disappeared, and there were differences between two groups (P < 0.05). Compared to the control group, white blood cells and red blood cells in feces of patients in the treatment group decreased significantly (P < 0.05). The number of plasmodium in peripheral blood of patients in the treatment group was significantly decreased. Conclusion: Wei Chang An Pills combined with Artemether Injection have the good effect in the treatment of malaria (gastrointestinal type), and can improve the clinical symptoms of patients and shorten the course, and also have anti-malarial function. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Zhang P.,Tianjin Zhongxin Pharmaceutical Group Co. | Wang X.,Tianjin Zhongxin Pharmaceutical Group Co. | Xiong S.,Tianjin Biochip Corporation | Wen S.,Tianjin Biochip Corporation | And 3 more authors.
Phytotherapy Research | Year: 2011

Zilongjin (ZLJ) tablet, which is a traditional Chinese medicine, has been approved as a new anti-tumor drug by the State Food and Drug Administration of China; however, its anti-cancer mechanisms remain elusive. The goal of this study was to investigate the underlying anti-cancer activities of ZLJ tablet in vitro. In this study, four lung cancer cell lines, A549, H446, H460 and H520, were treated with 2.2 mg/mL of ZLJ solution for 24 h at 37 °C under 5% CO 2. RNA was isolated and a microarray experiment using the Affymetrix Human Genome U133 plus 2.0 Array was employed to differentiate the expression patterns of cancer-related genes after drug treatment. Of 483 genes in 63 functional categories and 25 different pathways that showed at least a 2-fold change of expression level in the four cancer cell lines, 170 genes were upregulated, and 313 genes were downregulated. Eleven of the 483 genes were cancer-related and belong to the three known pathways: apoptosis, cell cycle regulation and mitogen-activated protein kinase (MAPK) cascade. The microarray data were validated by real-time RT-PCR. The results of this investigation suggest possible anti-cancer mechanisms of the ZLJ tablet, and lay a foundation to further analyse its therapeutic roles. Copyright © 2011 John Wiley & Sons, Ltd.


Chen Y.,Tianjin University of Traditional Chinese Medicine | Zheng H.,Tianjin University | Zhang J.,Tianjin University | Wang L.,Tianjin Zhongxin Pharmaceutical Group Co. | And 2 more authors.
RSC Advances | Year: 2016

The aim of the study was to investigate the protective effects of costunolide (Co) and dehydrocostus (De) in 5-fluorouracil (5-FU)-induced intestinal mucositis (IM) as well as the potential mechanisms involved. Male Kunming mice were given 5-FU (60 mg kg-1 per day) by intraperitoneal injections for 5 consecutive days and IM was evaluated histochemically. Co (5, 20 mg kg-1) and De (5, 20 mg kg-1) were orally administered once daily for 8 days. Repeated 5-FU treatment caused severe IM including morphological damage, which was accompanied by feeding reduction, body weight loss and diarrhea. Daily intragastric administration of Co or De significantly relieved the severity of IM through promoting intestinal mucosal recovery, inhibiting reactive oxygen species and ameliorating the inflammatory responses. Accordingly, Co and De may be promising therapeutic candidates and clinically used for the prevention of IM during cancer chemotherapy. © 2016 The Royal Society of Chemistry.


Bai X.-Y.,Hangzhou Normal University | Zhang P.,Tianjin Zhongxin Pharmaceutical Group Co. | Yang Q.,Hangzhou Normal University | Yang Q.,Chinese University of Hong Kong | And 8 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2014

Suxiao Jiuxin Pill, a compound Chinese traditional medicine with main components of tetramethylpyrazine and borneol, is widely used for antiangina treatment in China but its pharmacological effect on human blood vessels is unknown. We investigated the effect and possible mechanism of SJP in the human internal mammary artery (IMA, n = 78) taken from patients undergoing coronary surgery. SJP caused full relaxation in KCl- (99.4 ± 10.5 %, n = 6) and U46619- (99.9 ± 5.6 %, n = 6) contracted IMA. Pretreatment of IMA with plasma concentrations of SJP (1 mg/mL), calculated from the plasma concentration of its major component borneol, significantly depressed the maximal contraction to KCl (from 35.8 ± 6.0 mN to 12.6 ± 5.6 mN, P = 0.03) and U46619 (from 19.4 ± 2.9 mN to 5.7 ± 2.4 mN, P = 0.007) while SJP at 10 mg/mL abolished the subsequent contraction. Endothelium denudation and inhibition of eNOS significantly altered the SJP-induced relaxation without changes of eNOS expression. We conclude that SJP has a potent inhibitory effect on the vasoconstriction mediated by a variety of vasoconstrictors in human arteries. The vasorelaxation involves both endothelium-dependent and -independent mechanisms. Thus, the effect of SJP on human arteries demonstrated in this study may prove to be particularly important in vasorelaxing therapy in cardiovascular disease. © 2014 Xiao-Yan Bai et al.


Yang K.-L.,Tianjin University of Traditional Chinese Medicine | Wang Y.,Tianjin University of Traditional Chinese Medicine | Ai L.,Tianjin Zhongxin Pharmaceutical Group Co. | Yang J.,Tianjin Zhongxin Pharmaceutical Group Co. | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2015

Coronary artery disease (CAD) is a complex, multifactorial, chronic disease and one of the most dangerous diseases to human health due to its high mortality. The single target drug therapy is widely used in the treatment of CAD, but it could not meet the demand of today's treatment of complex disease. However, traditional Chinese medicine (TCM) has a good effect in treating complex disease. Tongmai Yangxin Prescription, based on "Zhigancao Decoction", is used to cure cardiovascular disease. The formulae is developed by Professor Ruan Shi-yi who won the title of "Guo Yi Da Shi (master of national medicine)". The studies on the single herb which consists in Tongmai Yangxin Prescription, such as Rehmanniae Radix and Glycyrrhizae Radix, have been made considerable progress. But systematic research of Tongmai Yangxin Prescription is still unavailable. There is a certain risk when we use Tongmai Yangxin Prescription in clinic, as the molecular basis of the formulae is unclear. The approach of network pharmacology is to set up a model of the compound action of Tongmai Yangxin Prescription. Meanwhile the model is based on the experimental data from single herb or active ingredients. Then, drug-target network, CAD-pathway network, and CAD-symptom network could be built to explain the functional mechanism through the method of literature mining and data mining, combining the theory of TCM. © 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


PubMed | Tianjin Zhongxin Pharmaceutical Group Co.
Type: Journal Article | Journal: Phytotherapy research : PTR | Year: 2011

Zilongjin (ZLJ) tablet, which is a traditional Chinese medicine, has been approved as a new anti-tumor drug by the State Food and Drug Administration of China; however, its anti-cancer mechanisms remain elusive. The goal of this study was to investigate the underlying anti-cancer activities of ZLJ tablet in vitro. In this study, four lung cancer cell lines, A549, H446, H460 and H520, were treated with 2.2mg/mL of ZLJ solution for 24h at 37C under 5% CO(2) . RNA was isolated and a microarray experiment using the Affymetrix Human Genome U133 plus 2.0 Array was employed to differentiate the expression patterns of cancer-related genes after drug treatment. Of 483 genes in 63 functional categories and 25 different pathways that showed at least a 2-fold change of expression level in the four cancer cell lines, 170 genes were upregulated, and 313 genes were downregulated. Eleven of the 483 genes were cancer-related and belong to the three known pathways: apoptosis, cell cycle regulation and mitogen-activated protein kinase (MAPK) cascade. The microarray data were validated by real-time RT-PCR. The results of this investigation suggest possible anti-cancer mechanisms of the ZLJ tablet, and lay a foundation to further analyse its therapeutic roles.


PubMed | Tianjin University of Traditional Chinese Medicine, Tianjin University and Tianjin Zhongxin Pharmaceutical Group Co.
Type: | Journal: Journal of ethnopharmacology | Year: 2016

Wei-Chang-An pill (WCA pill), a traditional Chinese pharmaceutical preparation, possessed potential anti-inflammatory advantages and noteworthy gastrointestinal regulations in digestive diseases, which might represent a promising candidate for the treatment of intestinal mucositis (IM) induced by 5-fluorouracil (5-FU).To analyze the bioactive constituents and investigate the effect of methanol extraction from WCA pill (WCA ext) on 5-FU induced IM with underlying mechanisms.The analysis of serum bioactive constituents after WCA ext administration in rats was carried out by UHPLC-Quadrupole-Time of Flight-Mass Spectrometry. In mice, IM was induced by 5-FU and physical manifestations were measured during the period of drug delivery. Half of mice were assessed with histology, expression of inflammatory cytokines in ileum and plasma via hematoxylin and eosin staining, immunohistochemical staining as well as cytokine enzyme-linked immunosorbent assay test, respectively. Besides, gastric emptying (GE) and gastrointestinal transit (GIT) were further tested in the other half of 5-FU induced mice.Twenty-two compounds were identified or tentatively characterized. IM induced by 5-FU was improved significantly after treatment with WCA ext through reducing the body weight loss, relieving the severe diarrhea, and inhibiting the GE as well as GIT. Further assessments validated that WCA ext promoted the recovery of intestinal mucosa, evaluated the activity of enterocyte proliferation, maintained the integrity of tight junction, and ameliorated the inflammatory disturbances.These results suggested that WCA ext promoted the restoration of intestinal function in 5-FU-induced IM via regulating multiple sites of actions in intestinal homeostasis. Accordingly, WCA pill might be a promising therapeutic candidate for the prevention of IM during cancer chemotherapy.


PubMed | Tianjin Zhongxin Pharmaceutical Group Corporation LTD. and Tianjin Medical University
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2016

Guchang capsule (GC) is a Chinese materia medica standardized product extracted from 15 Chinese traditional medical herbs and it has been clinically used in the treatment of intestinal disease. In this study, in order to extend the research of GC in intestinal disease, we were aiming to evaluate potential effects of GC on dextran sulphate sodium- (DSS-) induced murine experimental colitis and to elucidate the underlying mechanisms. GC treatment attenuated DSS-induced body weight loss and reduced the mortality. Moreover, GC treatment prevented DSS-induced colonic pathological damage; meanwhile it inhibited proinflammatory cytokines production in colon tissues. In vitro, GC significantly reduced LPS-induced proinflammatory cytokines production via inhibiting the activation of NF-B in macrophage cells, and the expressions of several long noncoding RNAs (lncRNAs) which were reported in regulating NF-B signaling pathway were obviously affected by adding GC into culture medium. In conclusion, our data suggested that administration of GC exhibits therapeutic effects on DSS-induced colitis partially through regulating the expression of NF-B related lncRNAs in infiltrating immune cells.

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