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Xie H.,Karolinska Institutet | Zhao Y.,Karolinska Institutet | Zhao Y.,Tianjin University of Sport | Caramuta S.,Karolinska Institutet | And 2 more authors.
PLoS ONE | Year: 2012

MicroRNAs (miRNAs) are short non-coding RNA regulators that control gene expression mainly through post-transcriptional silencing. We previously identified miR-205 in a signature for human cervical cancer using a deep sequencing approach. In this study, we confirmed that miR-205 expression was frequently higher in human cervical cancer than their matched normal tissue samples. Functionally, we demonstrate that miR-205 promotes cell proliferation and migration in human cervical cancer cells. To further understand the biological roles of miR-205, we performed in vivo crosslinking and Argonaute 2 immunoprecipitation of miRNA ribonucleoprotein complexes followed by microarray analysis (CLIP-Chip) to identify its potential mRNA targets. Applying CLIP-Chip on gain- and loss-of-function experiments, we identified a set of transcripts as potential targets of miR-205. Several targets are functionally involved in cellular proliferation and migration. Two of them, CYR61 and CTGF, were further validated by Western blot analysis and quantification of mRNA enrichment in the Ago2 immunoprecipitates using qRT-PCR. Furthermore, both CYR61 and CTGF were downregulated in cervical cancer tissues. In summary, our findings reveal novel functional roles and targets of miR-205 in human cervical cancer, which may provide new insights about its role in cervical carcinogenesis and its potential value for clinical diagnosis. © 2012 Xie et al. Source


Liu S.-S.,CAS Institute of Zoology | Liu S.-S.,Tianjin University of Sport
Annals of the New York Academy of Sciences | Year: 2010

We examined the intrinsic relation between two interdependent and interacted processes, namely, chemiosmotic energy coupling partition and redox signaling involved in mitochondrial respiration. The following aspects of research were conducted and discussed: generation sites and release sidedness of superoxide from the Q cycle of complex III of the mitochondrial respiratory chain; the different physiological roles of PMF components, ΔΨ and ΔpH (ΔpHS), of the Q cycle in mitochondrial superoxide generating and partitioning; and direct feedback effects of Q cycle-derived O2 •- on PMF energy partition through its interaction with protons in ΔpHS to form HO2 •, leading to decreasing ΔpHS and ATP synthesis due to its increasing effects of basic proton leak of mitochondria. The present experimental data give new evidence for our hypothesis of reactive oxygen species cycle cooperation with Q cycle and H+ cycle in respiratory chain in keeping PMF energy partition and its equilibrium with redox signaling regulation of mitochondrial respiration. © 2010 New York Academy of Sciences. Source


Ji L.L.,University of Minnesota | Zhang Y.,Tianjin University of Sport
Free Radical Research | Year: 2014

Contraction-induced production of reactive oxygen species (ROS) has been implicated in oxidative stress to skeletal muscle for the past few decades. As research advances more evidence has revealed a more complete role of ROS under both physiological and pathological conditions. The current review postulated that moderate intensity of physical exercise has antioxidant and anti-inflammatory effects due to the operation and cross-talks of several redox-sensitive signal transduction pathways. The functional roles and mechanisms of action of the nuclear factor κB, mitogen-activated protein kinase, and peroxisome proliferator-activated receptor γ co-activator 1α are highlighted. © 2013 Informa UK, Ltd. Source


Tan S.,Tianjin University of Sport | Li W.,Tianjin University of Finance and Economy | Wang J.,University of Southern Queensland
Journal of Sports Science and Medicine | Year: 2012

This study evaluated the effects of a 6-month combined aerobic and resistance training program on the body composition, glycemic control, lipid profile, and functional capacity of older patients with a long history of type 2 diabetes. 25 subjects (65.9 ± 4.2 yrs; M/F: 13/12) with a long history of type 2 diabetes (16.7 ± 6.7 yrs) were randomly allocated into either the exercise or control groups. The exercise group trained three sessions a week. Each session consisted of a warm-up period, 30 minutes of moderate aerobic exercise, 10 minutes of resistance training with five leg muscle exercises (two sets of 10-12 repetitions at 50-70% of 1RM for each activity), and a cool-down period. The variables of body composition, glycemic control, lipid profile, and functional capacity were measured before and after the study period. Exercise training decreased waist-hip ratio and body fat of the trained subjects. Concentrations of fasting and 2- hour post-glucose challenge plasma glucose and serum insulin, and glycosylated hemoglobin decreased significantly in the exercise group. Exercise training improved the lipid profile and also increased the leg muscle strength and 6-minute walking distance of the trained subjects. The control group, however, increased their body fat and fasting plasma glucose, while other variables were not changed during the study period. The current results demonstrate that elderly patients with a long history of type 2 diabetes can benefit from the 6-month combined aerobic and resistance training program. © Journal of Sports Science and Medicine (2012). Source


Liu W.,Tianjin University of Sport | Zhou C.,Shanghai University of Sport
Psychoneuroendocrinology | Year: 2012

Both chronic mild stress and an injection of corticosterone induce depression-like states in rodents. To further link mitochondrial dysfunction to the pathophysiology of major depression, here we describe two rat models of a depressive-like state induced by chronic unpredictable mild stress (CUMS) or corticosterone treatment (CORT). It is also a model that allows the simultaneous study of effects of exercise preconditioning on behavioral, electrophysiological, biochemical and molecular markers in the same animal. Exercise preconditioning ahead of CUMS and CORT treatment prevents many behavioral abnormalities resulted from CUMS. The changes in mitochondrial activity in brain and reduced expressions of superoxide dismutase (SOD1, SOD2), mitofusin (Mfn1, Mfn2) as well as brain-derived neurotrophic factor (BDNF) suggest that both CORT and CUMS may impair mitochondrial function and/or expressions of mitofusion and antioxidant enzymes that, in turn, may increase oxidative stress and reduce energy production in brain with depression-like behaviors. These findings suggest an underlying mechanism by which CORT, as well as CUMS, induces brain mitochondrial dysfunction that is associated with depressive-like states. Remarkably, physical exercise is identified as a helpful and preventive measure to promote mitochondrial function and expressions of mitofusin, BDNF and antioxidant enzymes in brain, so as to protect brain energy metabolism against CUMS, rather than the compound of corticosterone. © 2011 Elsevier Ltd. Source

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