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Shen Q.-F.,Tianjin Medical Union Center | Li H.-N.,Tianjin Medical Union Center | Xu T.-T.,Tianjin Medical Union Center | Xia Y.-P.,Tianjin Medical Union Center
National Medical Journal of China | Year: 2012

Objective: To explore the impairment mechanisms of blood brain barrier in spinal cord and observe the changes of matrix metalloproteinase-9 (MMP-9) and functional improvement in rats with chronic fluorosis. Methods: A total of 120 Wistar rats were divided randomly into 4 groups, high fluoride (fed by water with a high concentration of sodium fluoride at 200 mg/L), high fluoride control (fed by distilled water), defluorination (fed by water with a high concentration of sodium fluoride at 200 mg/L for 12 weeks and then distilled water for 12 weeks) and defluorination control (n=30 each). The urinary contents of fluoride were detect for 4 groups at Weeks 4, 8 and 12. The high fluoride and control groups were sacrificed at Week 12 while the defluorination and defluorination control groups at Week 24.Their cervical spinal cords were collected for electron microscope examinations. The expression of MMP-9 protein in thoracic cord was detected by immunohistochemistry and Western blot. Quantitative analysis of function of blood brain cord barrier was performed by the technique of Evans blue. The comparison of measurement data was performed with F test and correlation analysis. The cytological changes of neurons in thoracic spinal cord were detected after chronic fluorosis. Results: Under electron microscope, the pathological manifestations of chronic damage in blood brain barrier could be found. As compared with the high fluoride control group, the content of Evans blue increased markedly in spinal cord of the high fluoride group (29.2 ± 0.1 vs 0.7 ± 0.1 mg/L, P < 0.01). It was higher in the defluorination group than that in the defluorination control group. But there was no significant difference with the high fluoride group (29.2 ± 0.1 vs 28.9 ± 0.2 mg/L, P > 0.01). And the expression of MMP-9 increased in spinal cord of the fluorosis and defluorination groups in comparison with those in the control group. But no difference existed among them. Conclusion: The damage of blood brain barrier of spinal cord occurs probably as a result of a higher expression of MMP-9 in rats with chronic fluorosis. Defluorination for a short time may not recover. Source


Shen Q.,Tianjin Medical Union Center | Tian R.,Tianjin Medical Union Center | Li H.,Tianjin Medical Union Center | Xu T.,Tianjin Medical Union Center | Xia Y.,Tianjin Medical Union Center
National Medical Journal of China | Year: 2014

Objective: To explore the injury mechanism for white matter of spinal cord and the improvement of function after defluoriation. Methods: A total of 120 Wistar rats were separated randomly into 4 groups (n=30 each). High flouriod group received high concentration NaF water (200 mg/L) to establish fluorosis model; control group distilled water; deflourination group high concentration NaF water (200 mg/L) for 12 weeks and then distilled water for 12 weeks; defluorination control group. The urinary contents of fluoride were detected at Weeks 4, 8 and 12. The first two groups were sacrificed at Week 12 while the other two groups at Week 24. The spinal cord functions were detected by BBB scale and incline plate test. Their cervical spinal cord tissues were collected and observed under electron microscope. The expression of myelin basic protein (MBP) in thoracic cord was detected by immunohistochemistry and Western blot. The comparison of measurement data was performed with F test and correlation analysis. Cytological changes of white matter in spinal cord were detected after chronic fluorosis. Results The spinal functions of high flouriod and deflourination groups were inferior to those of the control groups. But no difference existed among the groups. Pathological manifestations of chronic white matter injury of spinal cord could be found in high flouriod and deflourination groups. The MBP expression in spinal cord of fluorosis and deflurination groups decreased in comparison with those in control groups. But no difference existed among them. Conclusion: White matter injury of spinal cord is present in chronic fluorosis rats. Defluoriation for a short time offers no recovery. Copyright © 2014 by the Chinese Medical Association. Source


Shen Q.,Tianjin Medical Union Center
Zhonghua yi xue za zhi | Year: 2014

OBJECTIVE: To explore the injury mechanism for white matter of spinal cord and the improvement of function after defluoridation.METHODS: A total of 120 Wistar rats were separated randomly into 4 groups (n = 30 each). High flouriod group received high concentration NaF water (200 mg/L) to establish fluorosis model; control group distilled water; defluoridation group high concentration NaF water (200 mg/L) for 12 weeks and then distilled water for 12 weeks; defluoridation control group. The urinary contents of fluoride were detected at Weeks 4, 8 and 12. The first two groups were sacrificed at Week 12 while the other two groups at Week 24. The spinal cord functions were detected by BBB scale and incline plate test. Their cervical spinal cord tissues were collected and observed under electron microscope. The expression of myelin basic protein (MBP) in thoracic cord was detected by immunohistochemistry and Western blot. The comparison of measurement data was performed with F test and correlation analysis. Cytological changes of white matter in spinal cord were detected after chronic fluorosis.RESULTS: The spinal functions of high flouriod and defluoridation groups were inferior to those of the control groups. But no difference existed among the groups. Pathological manifestations of chronic white matter injury of spinal cord could be found in high flouriod and defluoridation groups. The MBP expression in spinal cord of fluorosis and defluoridation groups decreased in comparison with those in control groups. But no difference existed among them.CONCLUSION: White matter injury of spinal cord is present in chronic fluorosis rats. Defluoridation for a short time offers no recovery. Source

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