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Liu Y.,Tianjin Medical University | Luo Y.,Tianjin Medical University | Wu J.,Tianjin Third Central Hospital | Wang Y.,Tianjin Medical University | And 5 more authors.
Scientific Reports

Graphene oxide (GO) has attracted enormous interests due to its extraordinary properties. Recent studies have confirmed the cytotoxicity of GO, we further investigate its mutagenic potential in this study. The results showed that GO interfered with DNA replication and induced mutagenesis at molecular level. GO treatments at concentrations of 10 and 100âμg/mL altered gene expression patterns at cellular level, and 101 differentially expressed genes mediated DNA-damage control, cell apoptosis, cell cycle, and metabolism. Intravenous injection of GO at 4âmg/kg for 5 consecutive days clearly induced formation of micronucleated polychromic erythrocytes in mice, and its mutagenesis potential appeared to be comparable to cyclophosphamide, a classic mutagen. In conclusion, GO can induce mutagenesis both in vitro and in vivo, thus extra consideration is required for its biomedical applications. Source

Chen X.-S.,Tianjin Medical University | Zhang Z.-T.,Tianjin Medical University | Du J.,Tianjin Medical University | Bi X.-C.,Tianjin Medical University | And 2 more authors.

Objective: To determine the optimal surgical margins in nephron-sparing surgery (NSS) for T1b renal cell carcinomas (RCC). Materials and Methods: We retrospectively assessed 87 T1b RCC specimens after radical nephrectomy through whole-kidney continuous sections, with 92 T1a RCCs included as controls. The completeness of pseudocapsule (PS) and extra-PS lesions and multifocality were microscopically examined, as was the greatest distance between extra-PS lesions and primary tumors. Results: The rates of incomplete PS (34% [30/87] vs 18% [17/92], P =.015) and positive cancer lesions beyond the PS (39% [34/87] vs 25% [23/92], P =.043) were significantly higher in the T1b than in the T1a group. All extra-PS lesions were located within 3.0 mm of the primary tumor. Multifocal tumors were found in 6% (5/87) of patients with T1b and 5% (5/92) of patients with T1a tumors (P =.928). Conclusion: These results indicate that 4 mm may be the optimal surgical margin for NSS for patients with T1b RCC because all extra-PS lesions were located within 3 mm of the primary tumors. © 2012 Elsevier Inc. All Rights Reserved. Source

Jia X.,Tianjin Medical University | Liu J.,Tianjin Medical University | Gao Y.,Institute of Hepatobiliary Disease | Huang Y.,Tianjin Medical University | Du Z.,Tianjin Third Central Hospital
Archives of Medical Research

Background and Aims: The diagnostic value of serum GPC3 in patients with hepatocellular carcinoma (HCC) remains controversial. Thus, we performed a systematic review and meta-analysis to assess the diagnostic accuracy of serum GPC3 for HCC. Methods: A systematic search was performed for the related studies. Sensitivity, specificity and other measures regarding the accuracy of serum GPC3 and alpha-fetoprotein (AFP) in the diagnosis of HCC were pooled using random-effects models. Summary receiver operating characteristic curve (sROC) analysis was used to summarize the overall test performance. Results: Nineteen studies were included in this meta-analysis. Pooled sensitivity, specificity and 95% confidence interval (CI) of serum GPC3 for the diagnosis of HCC were 55.2% (52.9-57.4%) and 84.2% (82.2-86.0%), respectively. When combining GPC3 with AFP, pooled sensitivity, specificity, and 95% CI were 75.7% (71.8-79.4%) and 83.3% (79.6-86.6%), respectively. The area under sROC (AUC) and 95% CI for AFP combined with GPC3 were 0.762 (0.649-0.875). For diagnosis of early HCC, pooled sensitivity and specificity of serum GPC3 were 55.1% (47.9-66.2%) and 97.0% (95.2-98.2%), respectively. The AUC of GPC3 for early HCC was 0.793 (0.668-0.917). Conclusions: This meta-analysis indicates that serum GPC3 has a comparable accuracy to AFP for the diagnosis of HCC, and there is an elevation in the sensitivity of diagnosis when GPC3 was combined with AFP. Diagnostic accuracy of serum GPC3 for early HCC is still unsatisfactory. © 2014 IMSS. Source

Wang H.,Tianjin Third Central Hospital | Liu D.,Institute of Acute Abdominal Diseases

Baicalin, as the main active ingredient of the root from Scutellaria, is usually used in the treatment of inflammatory diseases. In our study, we found that baicalin improved survival in septic mice in vivo and attenuated high-mobility group box 1 (HMGB1) and cytokine release from macrophages in vitro. The experiments in vitro showed that baicalin inhibited both viability of macrophages and the cell's secretion of HMGB1, tumor necrosis factor α, interleukin 6 (IL-6), and IL-1β induced by lipopolysaccharide. Meanwhile, experiments in vitro also indicated that baicalin did not affect the transcription and translation of HMGB1 but inhibit the cytoplasmic translocation of HMGB1 induced by lipopolysaccharide. We found that baicalin improved survival and tissue injury of septic mice in vivo. It also decreased serum HMGB1, tumor necrosis factor α, IL-6, and IL-1β in septic mice. In conclusion, baicalin inhibits the release of HMGB1 from macrophages and may be a potential therapeutic strategy for sepsis-related diseases. Copyright © 2014 by the Shock Society. Source

Li T.,Tianjin Third Central Hospital | Ma Q.,Tianjin Third Central Hospital | Ma Q.,Tianjin Medical University | Ning M.,Tianjin Third Central Hospital | And 2 more authors.
Molecular and Cellular Biochemistry

The objective of the study is to investigate the effect of hypoxic preconditioning on the immunomodulatory properties of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and the effect of cotransplantation of hUC-MSCs and human umbilical cord blood (hUCB)-derived CD34+ cells in a rabbit model of myocardial infarction. hUC-MSCs with or without hypoxic preconditioning by cobalt chloride were plated in a 24-well plate, and then cocultured with hUCB-CD34+ cells and PBMCs for 96 h at 37 C in a 5 % CO2 incubator. For the negative control, hUC-MSCs were omitted. The groups were divided as follows: A1 = HP-MSCs + hUCB-CD34 + cells + PBMC, A2 = hUC-MSCs + hUCB-CD34+ cells + PBMC, Negative Control = hUCB-CD34+ cells + PBMC. Culture supernatants of each group were collected, and the IL-10 and IFN-γ levels were measured by ELISA. A rabbit model of MI was established using a modified Fujita method. The animals were then randomized into three groups and received intramyocardial injections of 0.4 ml of PBS alone (n = 8, PBS group), hUC-MSCs in PBS (n = 8, hUC-MSCs group), or hUC-MSCs + CD34+ cells in PBS (n = 8, Cotrans group), at four points in the infarct border zone. Echocardiography was performed at baseline, 4 weeks after MI induction, and 4 weeks after cell transplantation, respectively. Stem cell differentiation and neovascularization in the infracted area were characterized for the presence of cardiac Troponin I (cTnI) and CD31 by immunohistochemical staining, and the extent of myocardial fibrosis was evaluated by hematoxylin and eosin (H&E) and Masson's trichrome. IFN-γ was 27.00 ± 1.11, 14.20 ± 0.81, and 7.22 ± 0.14 pg/ml, and IL-10 was 31.68 ± 3.08, 61.42 ± 1.08, and 85.85 ± 1.80 pg/ml for the Control, A1 and A2 groups, respectively, which indicated that hUCB-CD34+ cells induced immune reaction of peripheral blood mononuclear cells, whereas both hUC-MSCs and HP-MSCs showed an immunosuppressive effect, which, however, was attenuated by hypoxic preconditioning. The Cotrans group had less collagen deposition in the infarcted myocardium and better heart function than the hUC-MSCs or PBS group. The presence of cTnI-positive cells and CD31-positive tubular structures indicated the differentiation of stem cells into cardiomyocytes and neovascularization. The microvessel density was 12.19 ± 3.05/HP for the hUC-MSCs group and 31.63 ± 2.45/HP for the Cotrans group, respectively (P < 0.01). As a conclusion, both hUC-MSCs and HP-MSCs have an immunosuppressive effect on lymphocytes, which, however, can be attenuated by hypoxic preconditioning. Cotransplantation of hUC-MSCs and hUCB-CD34+ cells can improve heart function and decrease collagen deposition in post-MI rabbits. Thus, a combined regimen of hUC-MSCs and hUCB-CD34+ cells would be more desirable than either cells administered alone. This is most likely due to the increase of cardiomyocytes and enhanced angiogenesis in the infarcted myocardium. © 2013 Springer Science+Business Media New York. Source

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