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Zhu L.-J.,Nanjing Agricultural University | Ye Z.-L.,Tianjin Tasly Pride Pharmaceutical Co. | Guo Q.-S.,Nanjing Agricultural University | Lu Z.-M.,Jian Ginseng Research Institute
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To compare the quality of rust rot red ginseng and injured red ginseng, and thus provide the basis for ginseng cultivation and processing. Methods: Based on Chinese Pharmacopoeia and literature related to red ginseng, ten ginsenosides, total ash, acid-insoluble ash, ether extract, carbohydrate, and total protein of red ginseng in different transplanting systems were studied or determined. Results: The contents of total ash, acid-insoluble ash, volatile ether extract, and sucrose in rust rot red ginseng are significantly higher than those in normal red ginseng. The contents of ginsenoside Rg1, Rb1, Rc, Rb2, Re, Rf, Rb3, Rd, Rg2, and Rg3 in rust rot red ginseng and injured red gingseng are significantly lower than those in normal red ginseng. Conclusion: The raw materials of rust rot red ginseng and injured red ginseng have a significant effect on the quality of the processed red ginseng. ©, 2015, Editorial Office of Chinese Traditional and. All right reserved.


Zhang L.,Nanjing Agricultural University | Ye Z.,Tianjin Tasly Pride Pharmaceutical Co. | Guo Q.,Nanjing Agricultural University
Zhongguo Zhongyao Zazhi | Year: 2010

Objective: To study the effects of soil factors on the active components in Radix Ophiopogonis. Method: The content of polysaccharide, flavonoids, saponins, water-soluble extract and inorganic elements in Radix Ophiopogonis gathered from 7 different places were compared, physical and chemical properties and inorganic elements of soil were analyzed. The path and grey connection analysis were applied for studying the effects on the active components of Radix Ophiopogonis. Result and Conclusion: The concentrations of mineral elements in plant were mainly adjusted by active absorption. The active components in Radix Ophiopogonis was primarily effected by soil enzyme activity, the other important factors were potassium, pH,and organic matter. K,Fe,Mn,B,Ba,Zn of soil also had much influence on it then others inorganic elements.


Shen W.,Nanjing Agricultural University | Ye Z.,Nanjing Agricultural University | Ye Z.,Tianjin Tasly Pride Pharmaceutical Co. | Guo Q.,Nanjing Agricultural University
Zhongguo Zhongyao Zazhi | Year: 2010

Objective: To investigate the quality of Schisandra chinensis, and thus provide the useful reference data for its quality standard establishment and standardized cultivation. Method: Base on the Chinese Pharmacopoeia and literature relating to S. chinensis, the appearance, water-soluble extractive, total ash, acid-insoluble ash and volatile oil of 10 S. chinensis sampes collected from different producing sites in northease China were studied or determined, and the contents of major active lingans were measured by HPLC. Result: The content of total ash and acid-insoluble ash in S. chinensis was less than 7.0%, 1.0%, respectively. The contents of water-soluble extractive were higher than 45.0%. HPLC analyses showed that the content of schisandrol A was higher than 0.40%. All these results in accordance with the requirements of Chinese Pharmacopoeia were published in 2010. Conclusion: The differences of the appearance and the internal quality are different for the samples collected from different areas. And the establishment of a scientific and comprehensive quality standard system will be very important role in ensuring the quality of S. chinensis.


Zhang L.,Nanjing Agricultural University | Guo Q.,Nanjing Agricultural University | Ye Z.,Tianjin Tasly Pride Pharmaceutical Co.
Zhongguo Zhongyao Zazhi | Year: 2010

Objective: To determine the residues of organochlorine pesticides and heavy metals in Radix Ophiopogonis and Ophiopogon japonicus. Method: The residues of 4 isomers of benzene hexa chloride (BHC) and 4 isomers of dichloro dipheny trichloroethane (DDT) were determined by gas chromatography. The contents of Pb, Cd, Cu, Cr, Hg and As were determined by ICP. Result: The residues of organochlorine pesticides in Radix Ophiopogonis were lower than the permissible maximum limits of the Chinese national standard except hexachloride (BHC) in Radix Ophiopogonis from Cixi as well as Cu in soil of Luojiang. Conclusion: The enrichment capacity of Radix Ophiopogonis for (BHC) and Hg is higher. It is suggested that we should try to select herbs-growing soil for O. japonicus with a particular emphasis on the pesticides residues in soil.


Zhou D.,Shanghai University of Traditional Chinese Medicine | Zhou D.,Chinese National Institute for the Control of Pharmaceutical and Biological Products | Zhou D.,Tianjin Tasly Pride Pharmaceutical Co. | Tong L.,Tianjin Tasly Pride Pharmaceutical Co. | And 6 more authors.
Biomedical Chromatography | Year: 2011

A sensitive and reliable LC-ESI-MS method for simultaneous determination of nine ginsenosides (Rh1, Rg2, Rg1, Rf, Re, Rd, Rc, Rb2 and Rb1) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Hypersil Gold C18 column (100 × 2.1mm, 5μm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra- and inter-day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi-Qi-Fu-Mai injection. © 2010 John Wiley & Sons, Ltd.


Liu C.,China Pharmaceutical University | Ju A.,TianJin Tasly Pride Pharmaceutical Co. | Zhou D.,TianJin Tasly Pride Pharmaceutical Co. | Li D.,TianJin Tasly Pride Pharmaceutical Co. | And 3 more authors.
Molecules | Year: 2016

YiQiFuMai injection (YQFM) is a modern lyophilized powder preparation derived from the traditional Chinese medicine Sheng-mai san (SMS) used for treating cardiovascular diseases, such as chronic heart failure. However, its chemical composition has not been fully elucidated, particularly for the preparation derived from Ophiopogon japonicus. This study aimed to establish a systematic and reliable method to quickly and simultaneously analyze the chemical constituents in YQFM by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Sixty-five compounds in YQFM were tentatively identified by comparison with reference substances or literature data. Furthermore, twenty-one compounds, including three ophiopogonins, fifteen ginsenosides and three lignans were quantified by UFLC-IT-TOF/MS. Notably, this is the first determination of steroidal saponins from O. japonicus in YQFM. The relative standard deviations (RSDs) of intra- and inter-day precision, reproducibility and stability were ≥4.9% and all analytes showed good linearity (R2 ≥ 0.9952) and acceptable recovery of 91.8%-104.2% (RSD ≤ 5.4%), indicating that the methods were reliable. These methods were successfully applied to quantitative analysis of ten batches of YQFM. The developed approach can provide useful and comprehensive information for quality control, further mechanistic studies in vivo and clinical application of YQFM. © 2016 by the authors; licensee MDPI.


PubMed | Tianjin Tasly Group Co., Shenyang Pharmaceutical University and Tianjin Tasly Pride Pharmaceutical Co.
Type: Journal Article | Journal: Bioorganic & medicinal chemistry letters | Year: 2015

The total salvianolic acids are main effective constituents of Salvia miltiorrhiza Bge., a traditional Chinese medicine used for thousands of years. The purpose of present study was to make clear the composition and bioactivities of the minor components of the total salvianolic acids injection. As a result, three new minor phenolic acids (1-3) together with six known compounds (4-9) were characterized from the total salvianolic acids injection. Their structures were elucidated by extensive analysis of the spectral data. The absolute configuration of compounds 1-3 were confirmed by their J7,8 observed in (1)H NMR spectra, absorption band at approximately 250-260nm in their CD spectra as well as chemical shifts of C-8 and C-8 displayed in (13)C NMR spectra. Then DPPH free radical scavenging assay andquinine oxidoreductase 1 (NQO1) inducing activity test were employed to evaluate the antioxidant effect of new minor compounds 1 and 2. Compound 2 showed significant NQO1 inducing activity at 20M with IR value 2.6. Meanwhile, DPPH scavenging assay revealed that the inhibition rates of compounds 1 and 2 were 84.3% and 74.9% at 2mM, respectively.


PubMed | Tianjin University of Traditional Chinese Medicine, Guiyang College of Traditional Chinese Medicine and Tianjin Tasly Pride Pharmaceutical Co.
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

Reports show that, while the mechanism remains unknown, salvianolate lyophilized injection (SLI) improves functional recovery after stroke in diabetic rats. In this study, we investigated the mechanism and effect of SLI on stroke outcome in type 1 diabetic (T1DM) rats. T1DM were induced in adult male Wistar rats by injecting streptozotocin. T1DM rats were then subjected to 90 minutes of middle cerebral artery occlusion (MCAO). SLI (10.5, 21, 42 mg/kg, respectively) was administered by tail vein injection at 24 hours after MCAO, and dayly and last for 14 days. The neurological deficit score and brain infarct volume were assessed after 14 days. Also, VEGF, BDNF, TrkB, CREB and p-CREB levels in the ischemic brain tissue were analyzed with western blot at 14 days after MCAO. SLI significantly reduced neurological deficit scores and cerebral infarct volume, and reduced lesion volumes at all time points. SLI also increased the expression of VEGF, BDNF, TrkB, CREB and p-CREB protein levels in T1DM-MCAO rats. In summary, our results demonstrate that SLI can improve functional recovery after stroke in diabetic rats, and the mechanism of treating cerebral ischemic injury is related to the activation of the VEGF, BDNF-TrkB-CREB signaling pathway.


PubMed | TianJin Tasly Pride Pharmaceutical Co. and China Pharmaceutical University
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

YiQiFuMai injection (YQFM) is a modern lyophilized powder preparation derived from the traditional Chinese medicine Sheng-mai san (SMS) used for treating cardiovascular diseases, such as chronic heart failure. However, its chemical composition has not been fully elucidated, particularly for the preparation derived from Ophiopogon japonicus. This study aimed to establish a systematic and reliable method to quickly and simultaneously analyze the chemical constituents in YQFM by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Sixty-five compounds in YQFM were tentatively identified by comparison with reference substances or literature data. Furthermore, twenty-one compounds, including three ophiopogonins, fifteen ginsenosides and three lignans were quantified by UFLC-IT-TOF/MS. Notably, this is the first determination of steroidal saponins from O. japonicus in YQFM. The relative standard deviations (RSDs) of intra- and inter-day precision, reproducibility and stability were <4.9% and all analytes showed good linearity (R 0.9952) and acceptable recovery of 91.8%-104.2% (RSD 5.4%), indicating that the methods were reliable. These methods were successfully applied to quantitative analysis of ten batches of YQFM. The developed approach can provide useful and comprehensive information for quality control, further mechanistic studies in vivo and clinical application of YQFM.


PubMed | TianJin Tasly Pride Pharmaceutical Co. and China Pharmaceutical University
Type: Journal Article | Journal: Chinese journal of natural medicines | Year: 2016

Yi-Qi-Fu-Mai (YQFM) is extensively used clinically to treat cardiovascular diseases in China. To explore the anti-hypoxia effect of the extract of YQFM preparation (EYQFM), the EYQFM (1.4, 2.8, and 5.5 gkg(-1)d(-1)) was assessed for its heart-protective effect in a chronic intermittent hypoxia (CIH) animal model (oxygen pressure 7%-8%, 20 min per day) for 28 days of treatment. Betaloc (0.151 6 gkg(-1)d(-1)) was used as a positive control. The histopathological analyses of heart in CIH mice were conducted. Several cardiac state parameters, such as left ventricular ejection fractions (EF), stroke volume (SV), expression of creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and malondialdehyde (MDA) were measured. The results showed that treatment with EYQFM markedly reversed swelling of the endothelial cells and vacuolization in the heart when compared with the model group. Further study demonstrated that EYQFM significantly improved ventricular myocardial contractility by increasing EF and SV. In addition, EYQFM inhibited the activity of CK, LDH, decreased the level of MDA and improved SOD activity. The results demonstrated that EYQFM significantly improved the tolerability of myocardium to hypoxia and ameliorated the cardiac damage in the CIH model.

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