Tianjin Tasly Group Co.

Tianjin, China

Tianjin Tasly Group Co.

Tianjin, China
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Jing Y.-R.,Shenyang Pharmaceutical University | Zhou W.,Tianjin Tasly Group Co. | Li W.-L.,Tianjin Tasly Group Co. | Zhao L.-X.,Shenyang Pharmaceutical University | And 2 more authors.
Bioorganic and Medicinal Chemistry | Year: 2014

A group of novel taxoids, with modifications at C-7, C-10, C-3′ and C-14 positions of paclitaxel, was synthesized in order to improve their biological profile by decreasing their affinity with P-glycoprotein (P-gp) and increasing cellular permeability. Most of the new taxoids demonstrated the similar potent cytotoxic activities in MCF-7 human tumor cell line as paclitaxel in vitro. In the permeability assay with monolayers of Caco-2 cells, most of the compounds demonstrated an increased trans-cellular transport in A-to-B direction in comparison with paclitaxel. Among them the compounds T-13, T-15 and T-26 showed the highest permeability, and with efflux ratios better than that of ortataxel. The interaction of the compounds T-13 and T-26 with P-gp was evaluated using Madin-Darby canine kidney (MDCK)-multidrug resistance-1(MDR1) and MDCK-wild-type (WT). The results indicated that T-13 and T-26 were poor substrates for P-gp and possessed inhibiting effects of P-gp mediated efflux. It was thus clear that simultaneous modifications at the C-7, C-10 and C-3′ positions of paclitaxel significantly impaired its interactions with P-gp and interfered with P-gp mediated efflux. © 2013 Published by Elsevier Ltd. All rights reserved.


Li T.,Guangzhou University | Zhang L.,Guangzhou University | Tong L.,Tianjin Tasly Group Co. | Liao Q.,Guangzhou University
Biomedical Chromatography | Year: 2014

Baicalin is the main indicator for qualitative and quantitative analysis of Scutellaria baicalensis Georgi and its prescription in vivo and in vitro. Owing to its insolubility and instability, the analysis of baicalin in biological samples is analytically challenging. Although there have been many pharmacokinetic or metabolism studies on baicalin, the current reported sample pretreatment methods are not the optimal choice with regard to absolute recovery and operation procedure. Here we report a high-throughput salting-out-assisted homogeneous liquid-liquid extraction method with acetonitrile and ammonium sulfate. Eight kinds of commonly used salts, preferred salt concentration and auxiliary solvents were investigated. The extraction efficiency in the presence of ammonium salt and auxiliary solvent (methanol) in comparison to that from the salt-free aqueous increased to above 90%. The performance of the developed pretreatment method was further evaluated through testing specificity, linearity, precision, accuracy, extraction recovery and stability. In particular, the stability investigation results proved that the operation at low temperature would no longer necessary be for salting-out-assisted homogeneous liquid-liquid extraction compared with protein precipitation, and the pretreatment method would be valuable if the compounds were unstable within matrices. © 2013 John Wiley & Sons, Ltd.


Chen X.-P.,Tianjin University | Chen X.-P.,Tianjin Institute of Pharmaceutical Research | Chen X.-P.,Tianjin Tasly Group Co. | Li W.,Tianjin Tasly Group Co. | And 3 more authors.
Chinese Journal of Natural Medicines | Year: 2013

The roots of Angelica sinensis (RAS), are a Chinese herbal medicine traditionally used in prescriptions for replenishing blood, treating abnormal menstruation, and other women's diseases. It has also been widely marketed as health food for women's care in Asia, and as a dietary supplement in Europe and America. RAS is well-known for its hematopoietic, antioxidant, and immunoregulatory activities. RAS also possesses anti-cancer, memory, radioprotective, and neuroprotective effects. Phytochemical investigations on this plant led to organic acids, phthalides, polysaccharides, and other metabiolites. Based on recent animal studies and clinical trials, RAS has been used in the treatment of gynecologic diseases, cardio-cerebrovascular disease, nervous system diseases, and nephrotic syndrome. In this review, the recent phytochemical and pharmacological studies, drug-drug interactions, clinical applications, and toxicity of RAS are summarized. © 2013 China Pharmaceutical University.


Zhu Y.,Tianjin Tasly Group Co. | Tong L.,Tianjin Tasly Group Co. | Tong L.,Shenyang Pharmaceutical University | Zhou S.,Tianjin Tasly Group Co. | And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012

A rapid, sensitive and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of five active flavonoids (wogonin, chrysin, oroxylin A, naringenin, hesperetin) and four major alkaloids (berberine, coptisine, jatrorrhizine, palmatine) from Tang-Min-Ling-Pill in rat plasma. Plasma samples (100μL) were spiked with internal standards daidzein (for flavonoids) and tetrahydropalmatine (for alkaloids), acidified with HCl and extracted by liquid-liquid extraction with acetone. Chromatographic separation was performed on a Zorbax SB-C18 column with the mobile phase of water (containing 0.1% of formic acid)-acetonitrile (30:70, v/v) at a flow rate of 0.3mL/min in a run time of 7.0min. Detection was performed by multiple reaction monitoring mode using electrospray ionization in the positive ion mode. All analytes showed good linearity over the investigated concentration range (r>0.9900). The validated lower limit of quantification was 1.01ng/mL for wogonin and oroxylin A, 0.238ng/mL for chrysin, 1.01ng/mL for naringenin, 0.998ng/mL for hesperetin, 0.0505ng/mL for berberine, 0.0996ng/mL for coptisine, 0.0501ng/mL for jatrorrhizine, 0.0889ng/mL for palmatine, respectively. Intra- and inter-day precision (RSD%) was less than 15% and accuracy (RE%) ranged from -7.5% to 4.5%. The validated method was successfully applied to investigate the pharmacokinetics of the major flavonoids and alkaloids of Tang-Min-Ling-Pill after oral administration to rats. © 2012 Published by Elsevier B.V.


Tong L.,Shenyang Pharmaceutical University | Tong L.,Tianjin Tasly Group Co. | Zhou D.,Tianjin Tasly Group Co. | Zhou D.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of naringin, hesperidin, neohesperidin, naringenin and hesperetin in rat plasma, using liquiritin as the internal standard. Plasma samples extracted with a solid-phase extraction procedure were separated on a Zorbax SB-C18 analytical column (2.1 mm × 150 mm, 5 μm) and detected by electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The calibration curves were linear over the range of 3.0-600. ng/ml for naringin, 0.5-100. ng/ml for hesperidin, 3.5-700. ng/ml for neohesperidin, 5.0-1000. ng/ml for naringenin and hesperetin, respectively. The lower limits of quantification were 0.5. ng/ml for naringin, hesperidin, naringenin and hesperetin, and 0.35. ng/ml for neohesperidin. Intra- and inter-day precision (RSD%) was less than 15% and accuracy (RE%) ranged from -3.3% to 4.8%. The validated method was successfully applied to investigate the pharmacokinetics of the major flavanones of Fructus aurantii extract after oral administration to rats. © 2011.


Tong L.,Shenyang Pharmaceutical University | Tong L.,Tianjin Tasly Group Co. | Wan M.,Tianjin Tasly Group Co. | Zhang L.,Tianjin Tasly Group Co. | And 3 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of baicalin, wogonoside, baicalein, wogonin, oroxylin A and chrysin in rat plasma, using naringin as an internal standard. After acidifying with HCl, plasma samples were pretreated by liquid-liquid extraction with acetone. Chromatographic separation was accomplished on a Hypersil Gold-C18 analytical column (2.1×150mm, 5μm) utilizing a gradient elution profile and a mobile phase consisting of (A) 0.1% formic acid in water and (B) acetonitrile. Detection was performed by multiple reaction monitoring (MRM) mode using electrospray ionization in the positive ion mode. All analytes showed good linearity over the investigated concentration range (r>0.9900). The lower limit of quantification was 0.5ng/ml for baicalin, wogonoside, wogonin and oroxylin A, and 1.0ng/ml for baicalein and chrysin. Intra-day and inter-day precisions (RSD%) were less than 15% and accuracy (RE%) ranged from -6.7% to 5.8%. The validated method was successfully applied to investigate the pharmacokinetics of the major flavonoids of Radix scutellariae extract after oral administration to rats. © 2012 Elsevier B.V..


A pharmaceutical solution of taxanes, its preparation method, a composition comprising said solution and its pharmaceutical combination package are disclosed. Said pharmaceutical solution comprises taxanes, a pH regulator and a solvent, wherein the pH regulator is a water-soluble acid.


A pharmaceutical solution of taxanes, its preparation method, a composition comprising said solution and its pharmaceutical combination package are disclosed. Said pharmaceutical solution comprises taxanes, a pH regulator and a solvent, wherein the pH regulator is a water-soluble acid.


Patent
Tianjin Tasly Group Co. | Date: 2011-03-30

The present invention disclosed a crystalline of S-zopiclone having a powder X-Ray diffraction spectrum using Cu-Ka radiation with characteristic peaks expressed in terms of 2 at about 11.08, about 12.38, about 15.86, about 17.88, about 19.98 and about 20.58; a DSC thermogram with a peak at about 207.7C and an infrared absorption spectrum (IR) with characteristic peaks at about 3078cm^(-1), about 2942~2838cm^(-1), about 2790cm^(-1), about 1716cm^(-1), about 1463cm^(-1), about 1372cm^(-1) and about 757cm^(-1). The present invention also disclosed a method for preparing the crystalline of eszopiclone, its pharmaceutical composition and its use in preparing a medicament for treating sleep disorder.


The present invention relates to the field of medical technology. More specifically, the present invention relates to a preparation of taxanes for intravenous administration, which consists of two parts: a drug solution and an emulsion. Said drug solution consists of paclitaxel or docetaxel, a pH regulator and a solvent for injection, wherein said solvent for injection is an organic solvent. Said emulsion includes a fat emulsion and is composed of oil for injection, an emulsifier, an antioxidant, an isotonic regulator, a stabilizer, a pH regulator and water for injection. When used, the drug solution at the clinical dosage can be added and mixed homogeneously in the emulsion to perform intravenous drip directly; or the drug solution at the clinical dosage can also be firstly added into the emulsion with no less than 5 times volume of the drug solution and then a predetermined amount of normal saline or glucose solution for injection is added to perform intravenous drip. The preparation of the present invention does not contain solubilizer and has advantages of little toxicity, safety, effectiveness, stability and economy. The fat emulsion is also used as a nutritional replenishment, thus achieving a better therapeutic effect. In addition, the normal saline or glucose solution for injection can be used to replace a considerable amount of the emulsion, which makes the preparation, therefore, not only cost-efficient, but also convenient for transportation and storage in practice.

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