Wang Y.-G.,Tianjin Medical University |
Zhang C.,Tianjin Medical University |
Shen W.,Tianjin Taida Hospital |
Zheng C.,Tianjin Medical University |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Laminectomy for decompression can effectively play an effect on spinal nerve decompression. However, this operation can induce epidural fibrosis and fibers broke into the spinal canal, result in iatrogenic spinal stenosis, persistent or recurrent leg pain. Chlorogenic acid is one of the effective pharmacological ingredient of honeysuckle, and has anti-inflammatory effects. OBJECTIVE: To observe the effects of topical application of chlorogenic acid on epidural fibrosis and dural adhesion in rat models of laminectomy. METHODS: A total of 72 healthy adult Wistar rats were selected to prepare models of laminectomy, and randomly assigned to three groups (n=24). In the chlorogenic acid group, rats were administered chlorogenic acid 2 mL/rat before skin suture in the laminectomy area. Rats in the physiological saline group were given an equal volume of physiological saline. Rats in the blank control group were not given any treatment. Rats in each group were sacrificed at 4 weeks after model establishment. The general assessment and histological analysis were conducted. Hydroxyproline contents, interleukin-6 levels, transforming growth factor-β1 expression were measured. RESULTS AND CONCLUSION: Rats in each group were involved in the final analysis, dissected and sampled. In the chlorogenic acid group, less epidural collagen fibers proliferated and the appearance of fibers was normal. In the physiological saline and blank control groups, many epidural collagen fibers proliferated, and their appearance showed obvious scar tissue. Histological evaluation exhibited that the density of fibroblasts was lower in the chlorogenic acid group than in other two groups. Hydroxyproline contents of epidural fibrous scar were significantly lower in the chlorogenic acid group than in the physiological saline and blank control group (P < 0.01). RT-PCR results demonstrated that interleukin-6 levels and transforming growth factor-β1 expression were lower in the chlorogenic acid group than in the other two groups. These data indicated that the topical application of chlorogenic acid in rat models of laminectomy could inhibit fibroblast proliferation, reduce interleukin 6 and transforming growth factor-β1 expression, and prevent epidural scar adhesion. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.