Tianjin Sungene Biotech Co.

Tianjin, China

Tianjin Sungene Biotech Co.

Tianjin, China
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Ren L.,Nankai University | Meng M.,Nankai University | Wang P.,Nankai University | Xu Z.,Nankai University | And 4 more authors.
Talanta | Year: 2014

A rapid and sensitive fluorescence polarization immunoassay (FPIA), based on a polyclonal antibody, has been developed for the detection of sodium benzoate in spiked samples. The immunogen and fluorescein-labeled analyte conjugate were successfully synthesized, and the tracer was purified by TLC. Under the optimal assay conditions, the FPIA shows a detection range of 0.3-20.0 μg mL-1 for sodium benzoate with a detection limit of 0.26 μg mL-1 in the borate buffer. In addition, the IC50 value was 2.48 μg mL-1, and the cross-reactivity of the antibodies with ten structurally and functionally related analogs were detected respectively. Four kinds of food samples (energy drink, candy, ice sucker, RIOTM cocktail) were selected to evaluate the application of FPIA in real systems. The recoveries were 96.68-106.55% in energy drink; 95.78-100.80% in candy, 86.97-102.70% in ice sucker, and 103.58-109.87% in benzoate contained sample RIOTM cocktail, and coefficients of variation of this method were all lower than 11.25%. Comparing with the detection results of HPLC, the developed FPIA has comparative performance in the real sample determination. The results suggest that the FPIA developed in this study is a rapid, convenient and simple method, which is suitable to be used as a screening tool for homogeneous detection of sodium benzoate in food products. © 2013 Elsevier B.V.


Song Z.,Nankai University | Wang Y.,Nankai University | Dong Y.,Nankai University | Xu K.,Nankai University | And 7 more authors.
Analytical Methods | Year: 2016

For routine monitoring of the pharmacokinetic behavior of anticancer drug methotrexate (MTX), polyclonal antibodies for MTX were originally produced, and a sensitive chemiluminescence immunoassay (CLIA) was developed for the determination of plasma MTX. Three kinds of coupling reagents (EDC, CDI and isobutyl chloroformate) were utilized to synthesize MTX immunogens. The coupling ratio, titer and sensitivity of polyclonal antibodies for each immunogen were evaluated. Consequently, MTX-EDC-cBSA was found to be the optimal immunogen since it showed the highest coupling ratio and yielded antibodies with the highest sensitivity. Under optimal conditions, the developed CLIA showed a limit of detection (LOD) of 4.3 ng mL-1 in buffer and 9.1 ng mL-1 in plasma with acceptable coefficients of variations (<14.9%). The method exhibited no cross-reaction with the MTX metabolite (7-OH MTX) and structural analogs. When applied in a pharmacokinetic study, the CLIA results were statistically consistent with the HPLC method in measuring key pharmacokinetic parameters (t1/2, Cmax, AUC0-12 and MRT0-12). In conclusion, the CLIA method showed advantages of simple sample preparation, low cost, high sensitivity and good reproducibility. These properties make it a potential tool in the rapid detection of MTX for therapeutic drug monitoring (TDM). © The Royal Society of Chemistry 2016.


Dong Y.,Nankai University | Zhang J.,Tianjin Medical University | Xing Y.,Nankai University | Song Z.,Nankai University | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

As one of the rarely allowable azo dyes, ponceau 4R can be added in some foods in some countries. However, it is necessary to develop a credible and rapid analytical method for its monitoring, because of its potentially harmful risk. The hapten of ponceau 4R was first designed and synthesized by introducing a primary amine group into the structure of ponceau 4R. Based on the well-prepared hapten, the immunogen of ponceau 4R was prepared using glutaraldehyde to link ponceau 4R to the carrier protein. The triggered polyclonal antibody was obtained and tested by ELISA to optimize the proper dilution. An icELISA was developed for ponceau 4R, and the IC50 of the method is 36.82 ng/mL. The limit of detection is 0.80 ng/mL, and the linear range is 1-10000 ng/mL. Five selected structural analogues have no cross-reactivity with the anti-ponceau 4R polyclonal antibody (<0.3). In three food samples (grape juice, carbonated beverage, and RIO cocktail), the assay exhibits good stability and reproducibility with a recovery range of 93.87-103.77%, and the intra- and interassay coefficients of variation were <11.73%. The results indicate that the proposed icELISA is sensitive, accurate, specific, and simple, which provides an alternative for the detection of ponceau 4R in foods. © 2015 American Chemical Society.


Wang P.,Nankai University | Yin Y.,Nankai University | Eremin S.A.,Moscow State University | Rybakov V.B.,Moscow State University | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

An indirect immunoassay for the determination of vitamin B2 in food samples and vitamin tablets was developed. A carbodiimide-modified active ester method was used to synthesize the immunogen for vitamin B2. The coupling ratio of vitamin B2 to carrier protein in immunogen was 19.98:1. The titer of the polyclonal antibody was 1:64000, and the antibody showed high specificity in the presence of vitamin B2 photolytic products and other B group vitamins. The immunoassay showed detection limits (LODs) of 1.07 ng/mL in PBS, 24.6 ng/g in vitamin drink, and 0.50 mg/kg in milk powder. Recovery was 99.58-110.91% in milk powder and 70.20-100.5% in vitamin drink. Vitamin B2 samples were analyzed by high-pressure liquid chromatography (HPLC) and the immunoassay, and results showed good agreement. Finally, this method was applied to detect vitamin B2 in commercial milk powder and vitamin tablets, and the detected amount correlated well with the labeled amount. © 2013 American Chemical Society.


Xu Z.,Nankai University | Zheng L.,Nankai University | Yin Y.,Nankai University | Wang J.,Nankai University | And 6 more authors.
Food Control | Year: 2015

An enzyme immunoassay for erythrosine (Ery) in food products was developed in this study. Anti-Ery polyclonal antibody was obtained by immunizing rabbits with Ery-cationized BSA (cBSA) conjugates. Coupling ratios of erythrosine to carrier proteins were measured to be 16.6:1 in immunogen and 13.5:1 in coating antigen. The developed method showed high sensitivity with the IC50 value up to 29.1±6.79ngmL-1. In food samples (healthy energy drink, breezer, grape juice, coca-cola sugar, fermented bean curd and tomato paste), the limit of detection (LOD) values of the developed method ranged from 2.2ngmL-1 to 8.3ngmL-1. The recoveries ranged from 86.3% to 115.5%. Intra-assay and inter-assay variation were lower than 9.6% and 10.7% respectively. The method could specifically recognize Ery among commonly used food colors, and the results obtained with the proposed immunoassay were well related with that of the reference high performance liquid chromatography (R2>0.9999). Therefore, the proposed method could be selectively used for rapid screening Ery in the mentioned foodstuffs. © 2014 Elsevier Ltd.


Zhang B.,Jiangsu University | Du D.,Jiangsu University | Meng M.,Nankai University | Eremin S.A.,Moscow State University | And 4 more authors.
Analytical Letters | Year: 2014

Fluoroquinolones are effective antimicrobial agents, but their residues in food may cause serious health issues. Hence, it is necessary to develop a rapid and simple assay for fluoroquinolones. In this study, monoclonal antibodies against ciprofloxacin with broad specificity to fluoroquinolones were prepared. The newly developed magnetic particle-based competitive enzyme immunoassay was performed in a homogeneous sample, and ciprofloxacin was quantitatively detected in the range of 0.3-24.3 ng mL-1. The IC50 and LOD values of the method were 2.27 ng mL-1 and 0.25 ng mL-1. In chicken muscle, the recoveries of spiked ciprofloxacin at 8, 20, and 40 ng mL-1 were all higher than 79%. Additionally, the performance of the developed magnetic particle-based immunoassay exhibited an excellent correlation with commercial ELISA kits (r = 0.997). The anti-ciprofloxacin monoclonal antibodies provided high cross-reactivity with eight fluoroquinolones analogues: enrofloxacin (110.1%), sarafloxacin (99.7%), difloxacin (90.2%), danofloxacin (89.8%), norfloxacin (110.3%), lomefloxacin (65.2%), ofloxacin (75.1%), and flumequine (45.0%). This protocol provides an alternative immunoassay for ciprofloxacin with the advantages of simple separation, a homogeneous reaction, rapid detection (within 30 min), and stable results. Furthermore, it may be employed for the rapid general determination of fluoroquinolones in animal products. © 2014 Taylor & Francis Group, LLC.


Zhang L.,Nankai University | Song Z.,Nankai University | Dong Y.,Nankai University | Wang Y.,Nankai University | And 8 more authors.
Journal of Supercritical Fluids | Year: 2016

Chiral separation of seven commonly used 1,4-dihydropyridines (1,4-DHP) was achieved by supercritical fluid chromatography (SFC) on a immobilised polysaccharide chiral selectors coated with cellulose-tris(3,5-dichlorophenylcarbamate) (Chiralpak IC). In this method, isopropanol was used as a modifier and a maximum resolution of 13.38 was resulted. Nimodipine content of actual samples was determined through two-phase hollow fibre-based liquid-phase microextraction (2p-HF-LPME). Under optimal conditions, the limits of detection of the two nimodipine enantiomers were 0.3 and 0.5 μg cm-3. Recoveries of 80.0-99.8% were achieved. The developed SFC technique coupled with 2p-HF-LPME is a rapid, effective and environment-friendly method for separating and quantifying 1,4-DHP enantiomers. © 2015 Elsevier B.V. All rights reserved.


Yang L.,Nankai University | Gu W.,Nankai University | Gu W.,Tianjin Key Laboratory of Metal and Molecule Based Material Chemistry | Tian J.,Nankai University | And 9 more authors.
Inorganic Chemistry Communications | Year: 2013

A series of novel lanthanide-based coordination polymers, [Ln(L)(atpa)(H2O)2]n·1.5nH2O (HL = 4-[3-methyl-5-(pyridin-4-yl)-1,2,4-triazol-4-yl]benzoic acid, H 2atpa = 2-aminoterephthalic acid and Ln = Tb(1), Dy(2), Ho(3), Er(4), Tm(5), Yb(6)) have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction, optical and magnetic measurements. Compounds 1-6 are isomorphous, which can be described as a 2D network based on Ln 2(CO2)2 units. The magnetic properties of compound 1 (Tb) show typical antiferromagnetic interaction, while compound 2 (Dy) has ferromagnetic behavior. The room-temperature photoluminescence spectra of 1 exhibit strong characteristic emissions in the visible region, whereas compounds of Dy(2), Er(4) and Yb(6) display NIR luminescence upon irradiation at the ligand band. © 2013 Elsevier B.V.


Zhang B.,Jiangsu University | Zhang B.,Tianjin Bioradar Biotechnology Co. | Du D.,Jiangsu University | Meng M.,Nankai University | And 5 more authors.
Food Analytical Methods | Year: 2014

Amaranth (E 123) is a member of azo dyes, and it is allowed to use in various foods. The acceptable maximum addition of amaranth is strictly fixed because of its potential risk to physical health. The objective of this study was to prepare a specific anti-amaranth monoclonal antibody and develop an indirect competitive ELISA for amaranth quantification analysis. The immunogen and the coating antigen were designed by introducing a carboxyl group into amaranth for the conjugation with carrier proteins. Based on the immunogen, the monoclonal antibody exhibits satisfactory performances and the proposed ELISA shows an IC50 of 20.33 ng mL-1. The limit of detection is as low as 3.35 ng mL-1, and the linear standard curve of the method ranges from 3.0 to 243.0 ng mL-1. Additionally, the antibody reflects minimal cross-reactivity (<1 %) with six related food dyes (erythrosine, ponceau 4R, allura red, tartrazine, sunset yellow FCF, and brilliant blue). The recoveries of amaranth spiked beverage samples are in the range of 85.8-100.7 % with low coefficient of variation values (<11.5 %). The data shows that the developed ELISA provides a simple, sensitive, specific, and accurate alternative for amaranth determination and monitoring. Furthermore, it is the first time that icELISA of amaranth is developed based on monoclonal antibodies. © 2013 Springer Science+Business Media New York.


Zhang B.,Jiangsu University | Zhang B.,Tianjin Bioradar Biotechnology Co. | Du D.,Jiangsu University | Yin Y.,Nankai University | And 6 more authors.
Food Analytical Methods | Year: 2014

As a synthetic food dye, erythrosine is associated with serious toxicity in inducing thyroid tumors, and the use of erythrosine is strictly regulated in most counties including China. In this study, a direct enzyme-linked immunosorbent assay (ELISA) has been developed for analysis of erythrosine in food products. A highly specific monoclonal antibody (MAb) for erythrosine was produced using erythrosine–bovine serum albumin (BSA) conjugate as an artificial antigen, and horseradish peroxidase (HRP)-labeled MAb for erythrosine was utilized as the detection antibody. Under the optimized condition, the UV absorbance in microplate related well with erythrosine concentration in the range of 0.1–10.0 μg/g. The proposed method could be applied to determine erythrosine in beverage and cookie, with good recoveries (80 –103 %) for the three spiked levels (30, 50, and 100 μg/g), and the relative standard deviations of detected amount were <12.3 %. © 2014, Springer Science+Business Media New York.

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