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Yan Y.-B.,Peking University | Yan Y.-B.,Tianjin Stomatological Hospital | Duan D.-H.,Peking University | Zhang Y.,Peking University | Gan Y.-H.,Peking University
Medical Hypotheses | Year: 2012

The traumatic temporomandibular joint (TMJ) bony ankylosis has generated great interest in the cranio-maxillofacial surgeons yet remains an enigma, due to its unknown pathogenesis. Organization and ossification of hematoma is the classical hypothesis concerning the underlying pathophysiology, but it could not explain all the unique characters of TMJ bony ankylosis. The previous imaging descriptions about bony ankylosis tend to over-emphasize the obliteration of joint space and the overgrowth of new bone around the joint. Our recent study has found that the radiolucent zone in the bony fusion area indicating impaired bone healing is one of the most important imaging features of bony ankylosis, and this imaging feature is similar to that of hypertrophic nonunion of long bone. We also observe that there is close relationship between the mouth opening and the degree of calcification of radiolucent zone. Therefore, we hypothesize that the development of traumatic TMJ bony ankylosis may be the course of bone healing of two injured articular surfaces under the interference of opening movement, which is similar to the hypertrophic nonunion. Our hypothesis could help to explain some unintelligible characters of bony ankylosis, and deserves further studies. © 2011 Elsevier Ltd.


Liu H.,Chinese Institute of Microbiology and Epidemiology | Liu H.,No 307 Hospital Of Pla | Liang X.,Tianjin Stomatological Hospital | Wang D.,Chinese PLA General Hospital | And 6 more authors.
Shock | Year: 2015

Acute lung injury (ALI) is still a leading cause of morbidity and mortality in critically ill patients. Inhaled nitric oxide (NO) has been reported to ameliorate ALI. However, reactive nitrogen species produced by NO can cause lung injury. Because hydrogen gas (H2 ) is reported to eliminate peroxynitrite, it is expected to reduce the adverse effects of NO. Moreover, we have found that H2 inhalation can attenuate lung injury. Therefore, we hypothesized that combination therapy with NO and H2 might afford more potent therapeutic strategies for ALI. In the present study, a mouse model of ALI was induced by intratracheal administration of lipopolysaccharide (LPS). The animals were treated with inhaled NO (20 ppm), H2 (2%), or NO + H2 , starting 5 min after LPS administration for 3 h. We found that LPS-challenged mice exhibited significant lung injury characterized by the deterioration of histopathology and histologic scores, wet-to-dry weight ratio, and oxygenation index (ratio of oxygen tension to inspired oxygen fraction [PaO2/FIO2]), as well as total protein in the bronchoalveolar lavage fluid (BALF), which was attenuated by NO or H2 treatment alone. Combination therapy with NO and H2 had a more beneficial effect with significant interaction between the two. While the nitrotyrosine level in lung tissue was prominent after NO inhalation alone, it was significantly eliminated after breathing a mixture of NO with H2. Furthermore, NO or H2 treatment alone markedly attenuated LPS-induced lung neutrophil recruitment and inflammation, as evidenced by downregulation of lung myeloperoxidase activity, total cells, and polymorphonuclear neutrophils in BALF, as well as proinflammatory cytokines (tumor necrosis factor !, interleukins 1" and 6, and high-mobility group box 1) and chemokines (keratinocyte-derived chemokine, macrophage inflammatory proteins 1! and 2, and monocyte chemoattractant protein 1) in BALF. Combination therapy with NO and H2 had a more beneficial effect against lung inflammatory response. Moreover, combination therapy with NO and H2 could more effectively inhibit LPS-induced pulmonary early and late nuclear factor.B activation as well as pulmonary cell apoptosis. In addition, combination treatment with inhaled NO and H2 could also significantly attenuate lung injury in polymicrobial sepsis. Combination therapy with subthreshold concentrations of NO and H2 still had a significantly beneficial effect against lung injury induced by LPS and polymicrobial sepsis. Collectively, these results demonstrate that combination therapy with NO and H2 provides enhanced therapeutic efficacy for ALI. © 2015 by the Shock Society.


Peng P.,China Medical University at Heping | Peng P.,Tianjin Stomatological Hospital | Shen J.,Tianjin Stomatological Hospital | Dong J.-B.,Tianjin Medical University | Zhang Y.,China Medical University at Heping
International Journal of Oral and Maxillofacial Surgery | Year: 2014

The aim of this study was to evaluate the association of preoperative plasma fibrinogen levels with clinico-pathological parameters and disease-free survival in patients with oral tongue squamous cell carcinoma (OTSCC). We retrospectively studied 76 patients with OTSCC who underwent a partial glossectomy only, at a single centre, between 1996 and 2007. Among the 76 patients, 30 eventually developed cervical metastasis. Preoperative plasma fibrinogen levels were determined and correlated with clinico-pathological findings by t-test or analysis of variance methods. Univariate and multivariate analyses were used to determine the association of preoperative plasma fibrinogen levels and disease-free survival. Elevated levels of plasma fibrinogen were positively related with growth type (P < 0.001), differentiation (P < 0.001), thickness (P < 0.001), and the infiltrative growth ratio (P = 0.032). Univariate analysis showed that growth type (P < 0.001), differentiation (P < 0.001), thickness (P < 0.001), and preoperative plasma fibrinogen levels (P < 0.001) were significantly correlated with disease-free survival. Multivariate analysis showed that the plasma fibrinogen level remained an independent factor for disease-free survival after partial glossectomy for OTSCC (P = 0.029). A high preoperative plasma fibrinogen level is an independent predictor of cervical metastasis after partial glossectomy for OTSCC. A conservative supraomohyoid neck dissection is appropriate in patients with stage I/II carcinoma of the tongue whose preoperative plasma fibrinogen is >300 mg/dl. © 2013 International Association of Oral and Maxillofacial Surgeons.


Ji G.,Ninth Peoples Hospital | Liu D.,Shandong University | Liu J.,Qingdao University | Gao H.,Tianjin Stomatological Hospital | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2010

p38 MAPK and nuclear factor-B (NF-B) signaling pathways play an indispensable role in the control of skeletal myogenesis. The specific contribution of these signaling pathways to the response of myoblast to the mechanical stimulation and the molecular mechanisms underlying this response remain unresolved. Using an established in vitro model, we now show that p38 MAP kinase activity regulates the transcriptional activation of NF-κB in response to mechanical stimulation of myoblasts. Furthermore, SB203580 blocked stretch-induced NF-κB activation during myogenesis, not through down-regulation of degradation of IκB-α, and consequent translocation of the p65 subunit of NF-κB to the nucleus. It is likely that stretch-induced NF-κB activation by phosphorylation of p65 NF-κB. Moreover, depletion of p38α using siRNA significantly reduces stretch-induced phosphorylation of RelA and NF-κB activity. These results provides the first evidence of a cross-talk between p38 MAPK and NF-κB signaling pathways during stretch-induced myogenesis, with phosphorylation of RelA being one of the effectors of this promyogenic mechanism. The α isoform of p38MAP kinase regulates the transcriptional activation of NF-κB following stimulation with cyclic stretch. © 2009 Elsevier Inc.


Cao H.,Nihon University | Cao H.,Tianjin Stomatological Hospital | Kuboyama N.,Nihon University
Bone | Year: 2010

Polyglycolic acid (PGA) and beta-tricalcium phosphate (β-TCP) each have many applications as tissue repair materials. In this study, three-dimensional (3D) porous composite scaffolds of PGA/β-TCP (in 1:1 and 1:3 weight ratios) were fabricated using the solvent casting and particulate leaching method. PGA/β-TCP scaffolds with high porosity, interconnected 3D pores and rough surfaces were obtained and were observed using scanning electron microscopy (SEM) and micro-computed tomography (micro-CT). The PGA/β-TCP scaffolds were investigated during the repair of critical bone defects (3 mm diameter, 2 mm depth) in rat femoral medial-epicondyles, compared with hydroxylapatite (HAP) and no implant as controls. Quantitative imageology analysis (volume and density of new bone) and qualitative histological evaluations (hematoxylin and eosin staining; tartrate-resistant acid phosphatase-hematoxylin counterstaining) were characterized using in vivo micro-CT images and histological sections at 0, 14, 30 and 90 days after surgery. Significant differences of all variables were tested by multivariate analysis (p < 0.05). The results showed that the bone reformation by using the PGA/β-TCP scaffolds began within 14 days of surgery, and were healing well at 30 days after surgery. By 90 days after surgery, the bone replacement was almost completed and presented a healthy bone appearance. The new bone mineral densities (mg/cm 3) with HAP, PGA/β-TCP (1:1) and PGA/β-TCP (1:3) at 90 days after surgery were: 390.4 ± 18.1, 563.8 ± 26.9 and 606.3 ± 26.9, respectively. The new bone mineral density with the PGA/β-TCP scaffold was higher than with HAP (p < 0.001), and with the PGA/β-TCP (1:3) scaffold was higher than with the PGA/β-TCP (1:1) scaffold at each time examined (p < 0.05). The biodegradation percents (%) of HAP, PGA/β-TCP (1:1) and PGA/β-TCP (1:3) at 90 days after surgery were: 35.1 ± 5.5, 99.0 ± 1.0 and 96.2 ± 3.3, respectively. The biodegradation percents of the PGA/β-TCP scaffolds were higher than HAP at each time examined (p < 0.01), and matched the osteogenesis rates. The PGA/β-TCP scaffolds were almost replaced by new growing bone within 90 days after surgery. Thus the PGA/β-TCP composite scaffold, especially weight ratio 1:3, exhibited a strong ability for osteogenesis, mineralization and biodegradation for bone replacement. © 2009 Elsevier Inc. All rights reserved.


Zheng M.,University of Sichuan | Zheng M.,Zhoushan Hospital | Jiang Y.-P.,University of Sichuan | Chen W.,University of Sichuan | And 14 more authors.
Oncotarget | Year: 2015

MicroRNAs(miRNAs) can regulate epithelial-mesenchymal transition (EMT) through transcription factors, however, little is known whether EMT transcription factors can modulate miRNAs and further induce EMT and cancer metastasis. Here we show that overexpression of Snail and Slug leads to a mesenchymal phenotype and morphology and enhances cell invasion along with stem cell properties in squamous cell carcinoma of oral tongue (OTSCC) cells. Repression of miR-101 expression by Snail and Slug is essential for Snail/Slug-induced malignant phenotypes. The suppression of miR-101 subsequently activates EZH2, the sole histone methyltransferase, inducing EMT, migration and invasion of OTSCC cells. Importantly, co-overexpression of Slug and Snail correlates with poor survival and elevated EZH2 expression in two independent patient cohorts of OTSCC specimens. These findings defined a Snail and Slug/miR-101/EZH2 pathway as a novel regulatory axis of EMT-mediated-microRNA signaling.


Zhang J.,Tianjin Stomatological Hospital
Journal of Craniofacial Surgery | Year: 2016

ABSTRACT: Mucocele of the anterior lingual salivary glands is a more common cystic lesion, especially in patients aged less than 20 years. The study is aimed to observe the effect of treatment by injection of absolute ethanol instead of surgery. Fourteen outpatients diagnosed as mucocele of the glands of Blandin–Nuhn were selected. These patients, after blood investigation, were treated by injection of absolute ethanol into a mucous cavity of lesion under superficial anesthesia with 2% lidocaine once a week and followed up from 3 months to 2 years. Mucocele of the glands of Blandin–Nuhn was extirpated in all patients after 1 to 3 injections. There were no other complications except slight distending pain occurred on the same day when the patients were treated. The recurrence was not observed during the follow-up period. In conclusion, the study suggests that injection of absolute ethanol may be an alternative means for treating mucocele of the glands of Blandin–Nuhn, because it is mininvasive, safe, effective, economic, and simply manipulated compared with surgical treatment.This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially. http://creativecommons.org/licenses/by-nc-nd/4.0 © 2016 by Mutaz B. Habal, MD.


Wang H.W.,Tianjin Stomatological Hospital
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology | Year: 2012

To analyze the three-dimensional changes of pharyngeal airway in patients with skeletal Class III malocclusion after the combined orthodontic and orthognathic treatment. Twenty patients (9 males, 11 females) and 40 subjects with normal occlusion were involved in the study. Cone-beam computed tomography (CBCT) were performed on patients one week and six months after treatment. Raw data were reconstructed into three-dimensional model and sagittal and transversal measurements, cross sectional areas, partial and total volumes were computed. After treatment, the sagittal diameters of each section were significantly reduced by (2.7 ± 3.5), (3.0 ± 3.8), (2.7 ± 3.3) mm, respectively (P < 0.05). The transversal diameter of laryngopharyngeal airway constricted significantly by (4.8 ± 4.1) mm (P < 0.05). Cross sectional areas and volumes of each part in patients after surgery were significantly narrower compared with those of the controls. After combined therapy the pharyngeal airway space decreased.


Luo J.,Tianjin Medical University | Li Y.-N.,Tianjin Stomatological Hospital | Wang F.,Tianjin Medical University | Zhang W.-M.,Tianjin Medical University | Geng X.,Tianjin Medical University
International Journal of Biological Sciences | Year: 2010

A global DNA hypomethylation might activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-Adenosylmethionine (SAM) serves as a major methyl donor in biological transmethylation events. The object of this study is to explore the influence of SAM on the status of methylation at the promoter of the oncogenes c-,myc H-ras and tumor-suppressor gene p16 (INK4a), as well as its inhibitory effect on cancer cells. The results indicated that SAM treatment inhibited cell growth in gastric cancer cells and colon cancer cells, and the inhibition efficiency was significantly higher than that in the normal cells. Under standard growth conditions, C-myc and H-ras promoters were hypomethylated in gastric cancer cells and colon cancer cells. SAM treatment resulted in a heavy methylation of these promoters, which consequently downregulated mRNA and protein levels. In contrast, there was no significant difference in mRNA and protein levels of p16 (INK4a) with and without SAM treatment. SAM can effectively inhibit the tumor cells growth by reversing the DNA hypomet hyl ation on promoters of oncogenes, thus down-regulating their expression. With no influence on the expression of the tumor suppressor genes, such as P16, SAM could be used as a potential drug for cancer therapy. © Ivyspring International Publisher.


Liang S.-X.,Tianjin Stomatological Hospital | Yan Y.-B.,Tianjin Stomatological Hospital
Journal of Medical Hypotheses and Ideas | Year: 2014

Trauma is the leading cause of temporomandibular joint (TMJ) bony ankylosis. The treatment of the condition poses a significant challenge because of the high incidence of recurrence. We previously proposed a new view that the development of traumatic TMJ bony ankylosis may be a course similar to hypertrophic nonunion, and the ensuing animal experiments preliminarily verified this view through histological analysis and molecular biology examination. In view of the similarity between bone healing and bony ankylosis, and the importance of recruitment and differentiation of mesenchymal stem cells (MSCs) during the course of bone healing, it is reasonable to select MSCs as the breakthrough point for prevention of bony ankylosis. Recent studies reveal that fibroblast growth factor 21 (FGF21), a key mediator of peroxisome proliferator-activated receptor-γ (PPARγ), can promote adipocyte differentiation, inhibit osteoblast differentiation of MSCs and stimulate osteoclast activity by activation of PPARγ. Therefore, we hypothesize that local FGF21 injection may prohibit the onset of traumatic TMJ bony ankylosis through formation of a fat pad separating the condyle from the glenoid fossa, inhibition of new bone formation and promotion of bone resorption in the joint space, which thus may be a potential novel treatment for TMJ bony ankylosis. © 2013 Tehran University of Medical Sciences.

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