Xiuju C.,Tianjin Nankai hospital |
Zhen W.,Tianjin Nankai Hospital |
Yanchao S.,Tianjin port hospital
Open Medicine (Poland) | Year: 2016
Several studies have investigated the correla- tion between adiponectin gene rs22411766 T>G polymor- phism and ischemic stroke risk. However, the results were not conclusive with each other. Therefore, to overcome this obstacle, we performed this meta-analysis to further explicate the adiponectin gene rs22411766 T>G polymor- phism and ischemic stroke susceptibility. Case-control or cohort studies focused on adiponectin gene rs22411766 T>G polymorphism and ischemic stroke risk were elec- tronic searched in the databases of Medline, Pubmed, Cochrane library, Excerpta Medica database(EMBASE) and China National Knowledge Infrastructure (CNKI). All the potentially relevant studies were included in this meta-analysis. The association between adiponectin gene rs22411766 T>G polymorphism and ischemic stroke was expressed by odds ratio with its confidence interval. Publication bias has been assessed by begg's funnel plot. All the analyses have been performed by Revman 5.1 sta- tistical software. Finally, a total of six studies with 1,345 cases and 1,421 controls were included in this meta-analy- sis. Our results demonstrated that there was a significant association between adiponectin gene rs22411766 T>G polymorphism and ischemic stroke risk (p<0.05). People with G single nucleotide of adiponectin gene have the increased risk of developing ischemic stroke compared to T single nucleotide. © 2016 Chen Xiuju.
Liu G.,Shanxi Medical University |
Liang B.,Shanxi Medical University |
Song X.,Shanxi Medical University |
Bai R.,Shanxi Medical University |
And 5 more authors.
Molecular Medicine Reports | Year: 2016
Platelet activation is important in hypertension-induced cardiac inflammation and fibrosis. P-selectin expression significantly (P<0.05) increases when platelets are activated during hypertension. Although P-selectin recruits leukocytes to sites of inflammation, the role of P-selectin in cardiac inflammation and fibrosis remains to be elucidated. The present study aimed to investigate whether platelet-derived P-selectin promotes hypertensive cardiac inflammation and fibrosis. P-selectin knockout (P-sel KO) mice and wild-type (WT) C57BL/6 littermates were infused with angiotensin II (Ang II) at 1,500 ng/kg/min for 7 days and then cross-transplanted with platelets originating from either WT or P-sel KO mice. P-selectin expression was increased in the myocardium and plasma of hypertensive mice, and the P-sel KO mice exhibited significantly (P<0.05) reduced cardiac fibrosis. The fibrotic areas were markedly smaller in the hearts of P-sel KO mice compared with WT mice, as assessed by Masson's trichrome staining. In addition, α-smooth muscle actin and transforming growth factor β1 (TGF-β1) expression levels were decreased in the P-sel KO mice, as assessed by immunohistochemistry. Following platelet transplantation into P-sel KO mice, the number of Mac-2 (galectin-3)-and TGF-β1-positive cells was increased in mice that received WT platelets compared with those that received P-sel KO platelets, and the mRNA expression levels of collagen I and TGF-β1 were also increased. The results from the present study suggest that activated platelets secrete P-selectin to promote cardiac inflammation and fibrosis in Ang II-induced hypertension.
PubMed | Shanxi Medical University and Tianjin Port Hospital
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016
Platelet activation is important in hypertensioninduced cardiac inflammation and fibrosis. P-selectin expression significantly (P<0.05) increases when platelets are activated during hypertension. Although Pselectin recruits leukocytes to sites of inflammation, the role of Pselectin in cardiac inflammation and fibrosis remains to be elucidated. The present study aimed to investigate whether plateletderived Pselectin promotes hypertensive cardiac inflammation and fibrosis. Pselectin knockout (Psel KO) mice and wildtype (WT) C57BL/6 littermates were infused with angiotensinII (AngII) at 1,500ng/kg/min for 7days and then crosstransplanted with platelets originating from either WT or Psel KO mice. Pselectin expression was increased in the myocardium and plasma of hypertensive mice, and the Psel KO mice exhibited significantly (P<0.05) reduced cardiac fibrosis. The fibrotic areas were markedly smaller in the hearts of Psel KO mice compared with WT mice, as assessed by Massons trichrome staining. In addition, smooth muscle actin and transforming growth factor1 (TGF1) expression levels were decreased in the Psel KO mice, as assessed by immunohistochemistry. Following platelet transplantation into Psel KO mice, the number of Mac2 (galectin3) and TGF1positive cells was increased in mice that received WT platelets compared with those that received Psel KO platelets, and the mRNA expression levels of collagenI and TGF1 were also increased. The results from the present study suggest that activated platelets secrete Pselectin to promote cardiac inflammation and fibrosis in AngIIinduced hypertension.
Chen X.,Minzu University of China |
Zhuang S.,Minzu University of China |
Hou X.,Tianjin port hospital |
Liu Q.,Minzu University of China
Advanced Materials Research | Year: 2012
Neural stem cells (NSCs), as therapeutic agents, play the key role in the treatment of central nervous system (CNS) disorders. It is a researching tendency for promote proliferation and differentiation of neural stem cells by using tissue engineering. It is discovered that joint use neural stem cells and NSCs-seeded scaffold may increase the cell survival state and better control cellular microenvironment. We find suitable material include natural biological materials, synthetic materials, compound materials, bio-derived materials and 3D-materials of neural stem cells tissue engineering by analyzing and summing up of the research which combine tissue engineering with NSCs transplant in past several years. It hold out a hope of the possibility of utilizing the treatment of neural stem cells transplantation with tissue engineering, and there has been a great quantity of achievements on the research of it to treat CNS disorders by promote proliferation and differentiate. © (2012) Trans Tech Publications, Switzerland.
Liu Q.-S.,Minzu University of China |
Chen X.-Y.,Minzu University of China |
Chen X.-Y.,Tianjin Port Hospital |
Zhuang S.-J.,Minzu University of China |
Li K.-Q.,Minzu University of China
Zhongguo Zhongyao Zazhi | Year: 2013
The neural stem cells (NSCs), play a crucial role in stroke treatment, which can be regulated by a few of traditional Chinese medicines. In this study, the effect of the Mongolian medicine Baimai powder effective compounds group (BMECG) on the proliferation of NSCs has been investigated. The cultured NSCs which were isolated from newborn rat cerebral cortical in vitro were exposed to oxygen glucose deprivation/reoxgenation(OGD/R). The CFSE immunofluorescence staining was employed to identify the proliferation of NSCs by flow cytometry. Furthermore, the bilateral carotid artery occlusion (BCAO) was established on Kunming mice, and all groups were ig for 7 d respectively. The neurobehavioral changes was studied with rota-rod treadmill test, after that, the brain of mice were detected by immunohistochemistry with labeling of Nestin and pathological observation at 7 days after BCAO. It was found that, proliferation of NSCs was increased by BMECG in in vitro and in vivo. And BMECG significantly improved the time of staying in the rota-rod, it can promote the foundction of in cerebral cortex. It is concluded that these results further support the hypothesis that neuroprotective effect of BMECG may relate to the ability of stimulating self-renew of NSCs, which can be provided a new insight and strategy of anti-neuropathy of stroke.
Guo J.,Tianjin Medical University |
Guo J.,Tianjin Port Hospital |
Ye Z.-X.,Tianjin Medical University |
Feng X.-W.,Tianjin Medical University |
Yin Q.-L.,Tianjin Port Hospital
Chinese Journal of Medical Imaging Technology | Year: 2013
Objective: To observe MSCT findings of lung large cell neuroendocrine carcinoma (LCNEC). Methods: MSCT and pathologic findings of 27 patients with LCNEC were reviewed. All patients received plain CT , while 20 of them received enhanced CT scan. Results: LCNEC presented as single lung mass in 23 patients, while as multiple nodules of unilateral lung in 4 patients, which located peripherally in 26, but centrally only 1 patient, with diameters ranged from 1.2 to 14.2 cm (average [5.2±2.1]cm).The tumors were smooth and well defined in 20 cases (20/27, 74.07%), while necrosis was found in 9 cases (9/27, 33.33%)and calcification was detected in 2 cases (2/27, 7.41%). Lobulation of tumors was noticed in 19 cases (19/27, 70.37%), spiculation was observed in 13 cases (13/27, 48.15%), while vacuoles, pleural traction, obstructive atelectasis or pneumonia were found in 3(3/27, 11.11%), 7(7/27, 25.93%)and 4 cases(4/27, 14.81%), respectively. After injection of contrast agent, irregular enhancement of tumors was observed in all 20 cases that underwent enhanced CT scan. Mediastinal or hilar lymphadenectasis were observed in 14 cases (14/27, 51.85%), among which 8 cases were proved as metastasis. Adjacent chest wall invasion, bone metastasis or brain metastasis was detected in 1 case (1/27, 3.70%), respectively. While whole body metastasis was found in 1 case (1/27, 3.70%). Conclusion: The imaging features of LCNEC were very complicated and lack of characteristic. The final diagnosis depends on combining imaging and pathology. Copyright©2013 by the Press of Chinese Journal of Medical Imaging Technology.
Li W.-C.,Tianjin Port Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Antibiotic bone cement is the important method for the prevention and treatment of infection after artificial hip replacement and renovation. Objective: To review the research progress of antibiotic bone cement. Methods: A computer-based online search was performed in PubMed database, CNKI database, Chinese Biomedical Literature database, VIP database and Wanfang database for the literatures from 1978 to 2012. The key words were "bone cement, antibiotic bone cement, infection, joint replacement" in English and Chinese. Resutls and conclusion: A total of 335 literatures were screened out. Finally, 29 literatures were included for in-depth analysis after the primary screen through reading the title, abstract and full-text. Antibiotic bone cement has been widely used in the treatment of infection after artificial joint replacement and renovation as it can reduce the risk of infection after initial joint replacement and renovation. The material properties and mechanical properties will not change after bone cement mixed with appropriate amount of antibiotic. Different antibiotics in the bone cement have different release rates, which were closely related with the porosity of bone cement. Adding the additive that used for increasing the porosity of bone cement can increase the antibiotic release.
Ma W.-Z.,Tianjin Port Hospital |
Liang J.-F.,Tianjin Port Hospital |
Li W.-C.,Tianjin Port Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: The absorbable bone anchor is a new internal fixation method. Objective: To compare the clinical outcomes the metal splint external fixation and absorbable bone anchor system in treatment of closed mallet finger in early stage. Methods: Forty cases of closed mallet fingers were selected, 24 cases were treated with the metal splint external fixation and 16 cases were treated with absorbable bone anchor system. The time from injury to treatment was less than 1 week. The clinical data and surgical outcomes of the patients were retrospectively analyzed, and the excellent and good rate of two groups was compared with statistical analysis. Results and Conclusion: All patients were followed-up for 4-14 months. Reference to the International Institute of Hand Surgery recommended TAM system, the excellent and good rate was 66.7% in the metal splint external fixation group and 93.8% in the absorbable bone anchor system group, exhibiting a significant difference between two groups (P < 0.05). There were two cases of recurrence in the metal splints external fixation group, one at 4 months and the other at 6 months after treatment. There was one case of local swelling and skin redness n the absorbable bone anchor system during functional training and these symptoms were improved after physical therapy. It indicated that the effect of the absorbable bone anchor system is significantly better than that of the metal splint external fixation. Absorbable bone anchor system is the first choice for the treatment of early closed mallet finger with the simple surgical procedure and the certain effect. However, the expensive price limits its wider application. Metal splint is easy to operate, but difficult to manage and the relapse rate is high during treatment.
Chen J.-M.,Tianjin Port Hospital |
Li W.-C.,Tianjin Port Hospital |
Cai Y.,Tianjin Port Hospital |
Ding E.-Q.,Tianjin Port Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Proximal femoral fracture often occurs in elderly population, and selecting the appropriate fixation implants is the key to guarantee the biocompatibility and treatment effect. Objective: To analyze the selection and the biocompatibility of the metal implants for internal fixation for the treatment of proximal femoral fracture. Methods: The relative databases at home and abroad were searched by the first author for the relative articles in December 2012. The Chinese key words were "proximal femoral fracture, dynamic hip screw, less invasive stabilization system, percutaneous compression plate, Gamma nail, intramedullary nail", and the English key words were "proximal femoral fracture, dynamic hip screw, less invasive stabilization system, percutaneous compression plate, Gamma nail". The literatures of the articles were Chinese and English. A total of 142 articles were screened out, and 17 articles were included for the final analysis according to the inclusion criteria. Results and Conclusion: The methods for the treatment of proximal femoral fracture included conservative treatment, metal implants internal fixation, external fixation, locking plate and prosthesis replacement therapy. At present, the domestic and international treatment points have abandoned the conservative treatments such as traction, and try best to perform the metal implants internal fixation. The clinical metal implants internal fixation can be divided into extramedullary fixation device and intramedullary fixation device, and the extramedullary fixation device included dynamic hip screw, inverted distal femoral less invasive stabilization system and percutaneous compression plate, while the intramedullary fixation device included the proximal femoral intramedullary nail, Gamma nail and reconstruction nail. Each treatment method and internal fixation device has their own advantages and limitations. We should propose a personalized treatment program and select the appropriate method according to the patient's condition and treatment requirement during the clinical treatment.
Gao C.,Tianjin Medical University |
Fu X.,Tianjin Port Hospital |
Pan Y.,Tianjin Medical University |
Li Q.,Tianjin Medical University
Digestive Surgery | Year: 2010
Objectives: To review the clinical data of a group of patients with pancreatic neuroendocrine tumors (pNETs) and to investigate the role of surgery in the treatment for pNETs by analyzing clinical manifestations and postoperative course of this rare disease. Methods: A total of 112 patients (aged 21-76 years; 45 males) who underwent treatment between 1980 and 2003 were recruited in this study. Patients' data related to demographics and characteristics, diagnostic studies, surgical and tumor characteristics and survival were retrospectively reviewed. Results: Forty-six patients (41.1%) had a well-differentiated neuroendocrine tumor (WDT), 44 (48.2%) a well-differentiated neuroendocrine carcinoma (WD-Ca) and 12 (10.7%) a poorly differentiated neuroendocrine carcinoma (PD-Ca). Nonfunctional tumors were seen in 65 (58.0%) patients, whereas functional tumors were found in 47 (42.0%) patients, including 26 insulinomas, 17 gastrinomas, 2 VIPomas, 1 glucagonoma, and 1 ACTHoma. The sensitivity of computed tomography was 87.1%. Surgical resection was performed in 99 (88.4%) patients. Thirty-eight (33.9%) patients underwent partial pancreaticoduodenectomy, 32 (28.6%) had distal pancreatectomy and 29 (25.9%) underwent enucleation. No surgery-related death occurred. The common postoperative complications were pancreatic fistula (15.2%), wound infection (13.4%) and delayed gastric emptying (6.3%). Three (5%) patients had reoperation due to intra-abdominal abscess and postoperative hemorrhage. Twenty-six (55.3%) of the 47 functional tumors were malignant, whereas 40 (61.5%) of the 65 nonfunctional tumors were malignant. Survival was significantly related to the type of neuroendocrine tumor (p = 0.001). The overall 5-year actual survival rate of patients with WD-Ca (n = 54) was 56%, significantly less than that of patients with WDT (n = 46, 91%, p = 0.001). All the patients of PD-Ca (n = 12) group died in 5 years. The 5-year survival rate differed significantly between patients with tumor node metastasis (TNM) stage I and II disease and those with stage III and IV tumors (p = 0.011). Patients with stage III had better prognosis than those with stage IV tumors (p = 0.007). Patients' long-term survival was closely correlated with vascular invasion (p = 0.008) and resection margin (p = 0.004). Conclusions: PNETs can be safely resected. Microscopic vascular invasion and positive resection margin are helpful for predicting patient survival. Malignant cases should be treated with aggressive radical surgery to achieve complete tumor resection and potential for long-term survival. Copyright © 2010 S. Karger AG, Basel.