Zhang L.-L.,Tianjin University of Traditional Chinese Medicine |
Chu Q.,Tianjin University of Traditional Chinese Medicine |
Wang S.,Tianjin University of Traditional Chinese Medicine |
Lai H.,Tianjin University of Traditional Chinese Medicine |
Xie B.-B.,Tianjin Ninghe Hospital
Chinese Journal of Integrative Medicine | Year: 2016
Many clinical trials and experimental studies claim that sham acupuncture is as effective as traditional Chinese acupuncture. However, these studies have no standard sham acupuncture control and many other factors can affect the clinical effect. These factors include needle retention time, treatment frequency, and the total number of treatments needed for satisfactory results, and all can change the clinical effect. The majority of existing acupuncture treatment studies do not consider these factors and lack standard dosage criteria. Therefore, it is still too early to conclude that sham acupuncture is as effective as traditional Chinese acupuncture. This article investigates the factors that influence the curative effect of acupuncture as to help set a standard for acupuncture studies in the future. © 2016, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.
Tian X.,Tianjin Medical University |
Sun L.,Tianjin Medical University |
Wang J.,Tianjin Medical University |
Dong Y.,Tianjin Ninghe Hospital |
Zhan Z.,Tianjin Medical University
Chinese Journal of Clinical Oncology | Year: 2013
Objective: This work aimed to establish a lung squamous carcinoma cell line in which the coxsackievirus and adenovirus receptor (CAR) gene expression is stably suppressed by ribonucleic acid (RNA) interference. Colony formation experiments and orthotopic tumor model building were employed to detect the correlation between CAR and tumor formation. Methods: Three small interfering RNAs (siRNA) targeting the CAR gene were transfected into a lung squamous carcinoma NCI-H520 cell line. G418 was used to select a cell line where CAR gene expression was stably suppressed by RNA interference. The expression levels of CAR mRNA and protein were detected by Western blot in three NCI-H520 cell lines. The cell line where CAR gene expression was most suppressed was selected. Experiments on colony formation and tumorigenesis were employed to determine the effects of CAR gene on the proliferation of NCI-H520 cells after the CAR expression was reduced. Results: Three NCI-H520 cell lines wherein CAR gene expression was stably suppressed were obtained. Results of the colony formation assays suggested that the reduced CAR levels were associated with weakened cell proliferation. However, these data did not reach a statistical significance. The CAR-silenced NCI-H520 clones (CAR-) were inhibited in their ability to engraft the virocytes into the nude mice. Conclusions: A cell line NCI-H520 in which CAR gene expression was stably and efficiently suppressed was successfully constructed. The RNAi-mediated gene silencing of CAR can significantly affect the growth of NCI-H520 cells in the tumor-bearing nude mice. CAR is expected to become a new target for treating lung cancers.
Zhao W.,Tianjin Nankai Hospital |
Wang C.,Tianjin University of Traditional Chinese Medicine |
Li Z.,Tianjin University of Traditional Chinese Medicine |
Chen L.,Tianjin Ninghe Hospital |
And 9 more authors.
PLoS ONE | Year: 2015
Objective: This study was aimed at evaluating the clinical efficacy and safety of transcutaneous electrical acupoint stimulation (TEAS) to treat muscle spasticity after brain injury (Chinese Clinical Trial Registry: ChiCTR-TRC-11001310). Methods: A total of 60 patients with muscle spasticity after brain injury were randomized to the following 3 groups: 100, 2, and 0 Hz (sham) TEAS. The acupoints Hegu (LI4)-Yuji (LU10) and Zusanli (ST36)-Chengshan (BL57) on the injured side were stimulated at 0, 2, or 100 Hz, 5 times per week for 4 weeks. The patients were followed up for 1 and 2 months after the treatments. The effects of the treatments on muscle spasticity at the wrist, thumb, the other 4 fingers, elbow, shoulder, knee, and ankle were evaluated by the Modified Ashworth Scale, and the effects on disability were assessed by the Disability Assessment Scale. The walking capability was evaluated by the Holden functional ambulation classification score. The overall performance was assessed by the Global Assessment Scale score and the improved Barthel Index. The safety of the treatments administered was also monitored. Results: The wrist spasticity was significantly reduced from baseline at weeks 2, 3, and 4 of treatment and at the 1- and 2-month follow-up visits in the 100 Hz group (P < 0.01). Compared with 2 Hz or sham TEAS, 100 Hz TEAS decreased wrist spasticity at weeks 2, 3, and 4 of treatment and 1 month after treatment (P < 0.001). The other endpoints were not affected by the treatments. No treatment-emergent adverse events were reported during treatments and follow-up visits. Conclusions: TEAS appears to be a safe and effective therapy to relieve muscle spasticity after brain injury, although large-scale studies are required to further verify the findings. © 2015 Zhao et al.
Zhang F.,Tianjin Ninghe Hospital |
Meng Z.-P.,Tianjin Ninghe Hospital
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2014
This paper aims to compare the effect on early prognosis of post-traumatic early enteral nutrition therapy and central venous catheterization parenteral nutrition therapy in patients with severe traumatic brain injury (sTBI). The Results: showed that on 7 and 14 d, serum total protein [(62.04±2.09) and 66.04±2.27) g/L], albumin [(37.75±2.86) and (43.43±2.37) g/L] and prealbumin [(177.87±13.89) and (199.43±11.01) mg/L] in patients treated with early enteral nutrition were all higher than patients treated with parenteral nutrition (P=0.000, for all). On 7 d the stomach bleeding rate of patients treated with enteral nutrition (14.29%, 4/28) was lower than patients treated with parenteral nutrition (39.29%, 11/28), and the difference was statistically significant (χ2=4.462, P=0.035). On 14 d, no one case of stomach bleeding occured in patients with enteral therapy, while 4 cases of stomach bleeding (14.29%) occured in patients with parenteral therapy, and the difference was not significant (χ2=2.423, P=0.120). The difference of lung infection rate between different treated patients was not statistically significant (χ2=0.287, P=0.592). Early enteral nutrition therapy for severe traumatic brain injury patients can provide adequate nutritional support and reduce the incidence of stomach bleeding.